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Purification techniques

Expanding the DNA editing toolbox: Novel lambda integrase variants targeting microalgal and human genome sequences

Jia Wei Siau, Asim Azhar Siddiqui, Sze Yi Lau, Srinivasaraghavan Kannan, Sabrina Peter, Yingying Zeng, Chandra Verma, Peter Droge, John F. Ghadessy

published 13 Feb 2024

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An Open One-Step RT-qPCR for SARS-CoV-2 detection

Ariel Cerda, Maira Rivera, Grace Armijo, Catalina Ibarra-Henriquez, Javiera Reyes, Paula Blázquez-Sánchez, Javiera Avilés, Aníbal Arce, Aldo Seguel, Alexander J. Brown, Yesseny Vásquez, Marcelo Cortez-San Martín, Francisco A. Cubillos, Patricia García, Marcela Ferres, César A. Ramírez-Sarmiento, Fernán Federici, Rodrigo A. Gutiérrez

published 25 Jan 2024

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Methods optimization for the expression and purification of human calcium calmodulin-dependent protein kinase II alpha

Scott C. Bolton, David H. Thompson, Tamara L. Kinzer-Ursem

published 05 Jan 2024

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A universal protocol for high-quality DNA and RNA isolation from diverse plant species

Farhad Masoomi-Aladizgeh, Leila Jabbari, Reza Khayam Nekouei, Ali Aalami, Brian J. Atwell, Paul A. Haynes

published 14 Dec 2023

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Characterization and applications of glutaminase free L-asparaginase from indigenous Bacillus halotolerans ASN9

Ifrah Shafqat, Shaheen Shahzad, Azra Yasmin, Muhammad Tausif Chaudhry, Safia Ahmed, Aneela Javed, Imran Afzal, Monaza Bibi

published 28 Nov 2023

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Purification and characterization of novel isoforms of the polyphenol oxidase from Malus domestica fruit pulp

Naila Sajjad, M. Sheeraz Ahmad, Raja Tahir Mahmood, Muhammad Tariq, Muhammad Javaid Asad, Shamaila Irum, Anisa Andleeb, Abid Riaz, Dawood Ahmed

published 25 Aug 2023

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FAVIS: Fast and versatile protocol for non-destructive metabarcoding of bulk insect samples

Elzbieta Iwaszkiewicz-Eggebrecht, Piotr Łukasik, Mateusz Buczek, Junchen Deng, Emily A. Hartop, Harald Havnås, Monika Prus-Frankowska, Carina R. Ugarph, Paulina Viteri, Anders F. Andersson, Tomas Roslin, Ayco J. M. Tack, Fredrik Ronquist, Andreia Miraldo

published 19 Jul 2023

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Gradient-mixing LEGO robots for purifying DNA origami nanostructures of multiple components by rate-zonal centrifugation

Jason Sentosa, Franky Djutanta, Brian Horne, Dominic Showkeir, Robert Rezvani, Chloe Leff, Swechchha Pradhan, Rizal F. Hariadi

published 19 Jul 2023

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Purification of recombinant bacterial collagens containing structural perturbations

Sonal Gahlawat, Vikas Nanda, David I. Shreiber

published 17 May 2023

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Expression and secretion of glycosylated barley oxalate oxidase in Pichia pastoris

William Donelan, ShiWu Li, Paul R. Dominguez-Gutierrez, Augustus Anderson IV, Li-Jun Yang, Cuong Nguyen, Benjamin K. Canales

published 11 May 2023

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Isolation of salivary cell-free DNA for cancer detection

Patricia J. Brooks, Ethan Z. Malkin, Steven De Michino, Scott V. Bratman

published 02 May 2023

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Molecular and functional characterization of protease from psychrotrophic Bacillus sp. HM49 in North-western Himalaya

Hina Mushtaq, Shabir Ahmad Ganai, Arshid Jehangir, Bashir Ahmad Ganai, Rubiya Dar

published 30 Mar 2023

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Thermo-neutrophilic cellulases and chitinases characterized from a novel putative antifungal biocontrol agent: Bacillus subtilis TD11

Muhammad Saqib Malik, Abdul Rehman, Irfan Ullah Khan, Taj Ali Khan, Muhammad Jamil, Eui Shik Rha, Muhammad Anees

published 27 Jan 2023

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