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Mutagenesis

A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi

Christina S. Nødvig, Jakob B. Nielsen, Martin E. Kogle, Uffe H. Mortensen

published 15 Jul 2015

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Efficient Mutagenesis by Cas9 Protein-Mediated Oligonucleotide Insertion and Large-Scale Assessment of Single-Guide RNAs

James A. Gagnon, Eivind Valen, Summer B. Thyme, Peng Huang, Laila Ahkmetova, Andrea Pauli, Tessa G. Montague, Steven Zimmerman, Constance Richter, Alexander F. Schier

published 29 May 2014

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A Study on the Effect of Surface Lysine to Arginine Mutagenesis on Protein Stability and Structure Using Green Fluorescent Protein

Sriram Sokalingam, Govindan Raghunathan, Nagasundarapandian Soundrarajan, Sun-Gu Lee

published 09 Jul 2012

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Efficient CRISPR/Cas9-Mediated Gene Editing in Arabidopsis thaliana and Inheritance of Modified Genes in the T2 and T3 Generations

WenZhi Jiang, Bing Yang, Donald P. Weeks

published 11 Jun 2014

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C. elegans Mutant Identification with a One-Step Whole-Genome-Sequencing and SNP Mapping Strategy

Maria Doitsidou, Richard J. Poole, Sumeet Sarin, Henry Bigelow, Oliver Hobert

published 08 Nov 2010

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Improved Genome Editing in Human Cell Lines Using the CRISPR Method

Ivan M. Munoz, Piotr Szyniarowski, Rachel Toth, John Rouse, Christophe Lachaud

published 10 Oct 2014

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Golden GATEway Cloning – A Combinatorial Approach to Generate Fusion and Recombination Constructs

Stephan Kirchmaier, Katharina Lust, Joachim Wittbrodt

published 07 Oct 2013

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Improved Somatic Mutagenesis in Zebrafish Using Transcription Activator-Like Effector Nucleases (TALENs)

Finola E. Moore, Deepak Reyon, Jeffry D. Sander, Sarah A. Martinez, Jessica S. Blackburn, Cyd Khayter, Cherie L. Ramirez, J. Keith Joung, David M. Langenau

published 24 May 2012

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PFunkel: Efficient, Expansive, User-Defined Mutagenesis

Elad Firnberg, Marc Ostermeier

published 17 Dec 2012

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OmniChange: The Sequence Independent Method for Simultaneous Site-Saturation of Five Codons

Alexander Dennig, Amol V. Shivange, Jan Marienhagen, Ulrich Schwaneberg

published 19 Oct 2011

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A Simplified Method for Gene Knockout and Direct Screening of Recombinant Clones for Application in Paenibacillus polymyxa

Seong-Bin Kim, Salme Timmusk

published 27 Jun 2013

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BRED: A Simple and Powerful Tool for Constructing Mutant and Recombinant Bacteriophage Genomes

Laura J. Marinelli, Mariana Piuri, Zuzana Swigoňová, Amrita Balachandran, Lauren M. Oldfield, Julia C. van Kessel, Graham F. Hatfull

published 17 Dec 2008

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CRISPR/Cas9-mediated targeted mutagenesis in grape

Ikuko Nakajima, Yusuke Ban, Akifumi Azuma, Noriyuki Onoue, Takaya Moriguchi, Toshiya Yamamoto, Seiichi Toki, Masaki Endo

published 18 May 2017

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