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Enzyme purification

An Open One-Step RT-qPCR for SARS-CoV-2 detection

Ariel Cerda, Maira Rivera, Grace Armijo, Catalina Ibarra-Henriquez, Javiera Reyes, Paula Blázquez-Sánchez, Javiera Avilés, Aníbal Arce, Aldo Seguel, Alexander J. Brown, Yesseny Vásquez, Marcelo Cortez-San Martín, Francisco A. Cubillos, Patricia García, Marcela Ferres, César A. Ramírez-Sarmiento, Fernán Federici, Rodrigo A. Gutiérrez

published 25 Jan 2024

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Characterization and applications of glutaminase free L-asparaginase from indigenous Bacillus halotolerans ASN9

Ifrah Shafqat, Shaheen Shahzad, Azra Yasmin, Muhammad Tausif Chaudhry, Safia Ahmed, Aneela Javed, Imran Afzal, Monaza Bibi

published 28 Nov 2023

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Purification and characterization of novel isoforms of the polyphenol oxidase from Malus domestica fruit pulp

Naila Sajjad, M. Sheeraz Ahmad, Raja Tahir Mahmood, Muhammad Tariq, Muhammad Javaid Asad, Shamaila Irum, Anisa Andleeb, Abid Riaz, Dawood Ahmed

published 25 Aug 2023

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Expression and secretion of glycosylated barley oxalate oxidase in Pichia pastoris

William Donelan, ShiWu Li, Paul R. Dominguez-Gutierrez, Augustus Anderson IV, Li-Jun Yang, Cuong Nguyen, Benjamin K. Canales

published 11 May 2023

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Molecular and functional characterization of protease from psychrotrophic Bacillus sp. HM49 in North-western Himalaya

Hina Mushtaq, Shabir Ahmad Ganai, Arshid Jehangir, Bashir Ahmad Ganai, Rubiya Dar

published 30 Mar 2023

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Thermo-neutrophilic cellulases and chitinases characterized from a novel putative antifungal biocontrol agent: Bacillus subtilis TD11

Muhammad Saqib Malik, Abdul Rehman, Irfan Ullah Khan, Taj Ali Khan, Muhammad Jamil, Eui Shik Rha, Muhammad Anees

published 27 Jan 2023

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Starting from scratch: Step-by-step development of diagnostic tests for SARS-CoV-2 detection by RT-LAMP

Diana Angélica Tapia-Sidas, Brenda Yazmín Vargas-Hernández, José Abrahán Ramírez-Pool, Leandro Alberto Núñez-Muñoz, Berenice Calderón-Pérez, Rogelio González-González, Luis Gabriel Brieba, Rosalía Lira-Carmona, Eduardo Ferat-Osorio, Constantino López-Macías, Roberto Ruiz-Medrano, Beatriz Xoconostle-Cázares

published 26 Jan 2023

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ATP hydrolytic activity of purified Spf1p correlate with micellar lipid fluidity and is dependent on conserved residues in transmembrane helix M1

Johan Ørskov Ipsen, Danny Mollerup Sørensen

published 20 Oct 2022

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Formation of recombinant bifunctional fusion protein: A newer approach to combine the activities of two enzymes in a single protein

Patel Nilpa, Kapadia Chintan, R. Z. Sayyed, Hesham El Enshasy, Hala El Adawi, Alaa Alhazmi, Atiah H. Almalki, Shafiul Haque

published 01 Apr 2022

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Heterologous expression and purification of recombinant human protoporphyrinogen oxidase IX: A comparative study

Zora Novakova, Daria Khuntsaria, Marketa Gresova, Jana Mikesova, Barbora Havlinova, Shivam Shukla, Lucie Kolarova, Katerina Vesela, Pavel Martasek, Cyril Barinka

published 18 Nov 2021

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Extracellular proteolytic activation of Pseudomonas aeruginosa aminopeptidase (PaAP) and insight into the role of its non-catalytic N-terminal domain

Itschak Axelrad, Mary Safrin, Rivka Cahan, Sang-Jin Suh, Dennis E. Ohman, Efrat Kessler

published 16 Jun 2021

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Generation of 4-vinylguaiacol through a novel high-affinity ferulic acid decarboxylase to obtain smoke flavours without carcinogenic contaminants

Thorben Detering, Katharina Mundry, Ralf G. Berger

published 21 Dec 2020

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Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

R. Z. Sayyed, H. M. Bhamare, Sapna, Najat Marraiki, Abdallah M. Elgorban, Asad Syed, Hesham Ali El-Enshasy, Daniel J. Dailin

published 04 Jun 2020

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