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Synthetic biology

Evaluation of Peregrinus maidis transformer-2 as a target for CRISPR-based control

Yu-Hui Wang, Dina Espinoza Rivera, William Klobasa, Marcé D. Lorenzen

An efficient and cost-effective method for disrupting genes in RAW264.7 macrophages using CRISPR-Cas9

Mohammad J. Hossain, Tamara J. O’Connor

Comparative evaluation of bioinformatic tools for virus-host prediction and their application to a highly diverse community in the Cuatro Ciénegas Basin, Mexico

Alejandro Miguel Cisneros-Martínez, Ulises E. Rodriguez-Cruz, [ ... ], Valeria Souza

Predicting CRISPR-Cas12a guide efficiency for targeting using machine learning

Aidan O’Brien, Denis C. Bauer, Gaetan Burgio

Automation protocol for high-efficiency and high-quality genomic DNA extraction from Saccharomyces cerevisiae

Nina Alperovich, Benjamin M. Scott, David Ross

Functional analysis of Collagen 17a1: A genetic modifier of junctional epidermolysis bullosa in mice

Thomas J. Sproule, Robert Y. Wilpan, [ ... ], John P. Sundberg

Performance characteristics and operational feasibility assessment of a CRISPR based tata MD CHECK diagnostic test for SARS-CoV-2 (COVID-19)

Shubhada Shenai, Gita Nataraj, [ ... ], Sarabjit S. Chadha

Wikipedia as a tool for contemporary history of science: A case study on CRISPR

Omer Benjakob, Olha Guley, [ ... ], Rona Aviram

Gene knock-outs in human CD34+ hematopoietic stem and progenitor cells and in the human immune system of mice

Daniel A. Kuppers, Jonathan Linton, [ ... ], Patrick J. Paddison

The structure of a Type III-A CRISPR-Cas effector complex reveals conserved and idiosyncratic contacts to target RNA and crRNA among Type III-A systems

Mohammadreza Paraan, Mohamed Nasef, [ ... ], Jack A. Dunkle

Targeted deletion of grape retrotransposon associated with fruit skin color via CRISPR/Cas9 in Vitis labrascana ‘Shine Muscat’

Ikuko Nakajima, Hiroyuki Kawahigashi, [ ... ], Masaki Endo

TReSR: A PCR-compatible DNA sequence design method for engineering proteins containing tandem repeats

James A. Davey, Natalie K. Goto

Optimization of gene editing in cowpea through protoplast transformation and agroinfiltration by targeting the phytoene desaturase gene

Aya Bridgeland, Sudip Biswas, [ ... ], Endang M. Septiningsih

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