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Transfection

High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice

Jin Hee Kim, Sang-Rok Lee, [ ... ], Seok-Yong Choi

Exon Exchange Approach to Repair Duchenne Dystrophin Transcripts

Stéphanie Lorain, Cécile Peccate, Maëva Le Hir, Luis Garcia

The Miraprep: A Protocol that Uses a Miniprep Kit and Provides Maxiprep Yields

Mira I. Pronobis, Natalie Deuitch, Mark Peifer

A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells

Eric Campeau, Victoria E. Ruhl, [ ... ], Paul D. Kaufman

Construction of an infectious horsepox virus vaccine from chemically synthesized DNA fragments

Ryan S. Noyce, Seth Lederman, David H. Evans

A Comparative Study of Protocols for Mouse Embryonic Stem Cell Culturing

Christoffer Tamm, Sara Pijuan Galitó, Cecilia Annerén

Optimization of Genome Engineering Approaches with the CRISPR/Cas9 System

Kai Li, Gang Wang, [ ... ], William T. Pu

Functional Activity of Plasmid DNA after Entry into the Atmosphere of Earth Investigated by a New Biomarker Stability Assay for Ballistic Spaceflight Experiments

Cora S. Thiel, Svantje Tauber, [ ... ], Oliver Ullrich

An Efficient Low Cost Method for Gene Transfer to T Lymphocytes

Leonardo Chicaybam, Andressa Laino Sodre, Bianca Azevedo Curzio, Martin Hernan Bonamino

A Flow Cytometry-Based FRET Assay to Identify and Analyse Protein-Protein Interactions in Living Cells

Carina Banning, Jörg Votteler, [ ... ], Michael Schindler

An Enhanced Monomeric Blue Fluorescent Protein with the High Chemical Stability of the Chromophore

Oksana M. Subach, Paula J. Cranfill, Michael W. Davidson, Vladislav V. Verkhusha

A New Mesenchymal Stem Cell (MSC) Paradigm: Polarization into a Pro-Inflammatory MSC1 or an Immunosuppressive MSC2 Phenotype

Ruth S. Waterman, Suzanne L. Tomchuck, Sarah L. Henkle, Aline M. Betancourt

A Rapid and Economic In-House DNA Purification Method Using Glass Syringe Filters

Yun-Cheol Kim, Sherie L. Morrison

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