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Nucleic acid thermodynamics

A Comprehensive Evaluation of PCR Primers to Amplify the nifH Gene of Nitrogenase

John Christian Gaby, Daniel H. Buckley

Emulsion PCR: A High Efficient Way of PCR Amplification of Random DNA Libraries in Aptamer Selection

Keke Shao, Weifeng Ding, [ ... ], Huimin Wang

DNA Extraction from Dry Museum Beetles without Conferring External Morphological Damage

M. Thomas P. Gilbert, Wendy Moore, Linea Melchior, Michael Worobey

Monitoring the T-Cell Receptor Repertoire at Single-Clone Resolution

Hendrik P.J. Bonarius, Frank Baas, [ ... ], Niek de Vries

PFunkel: Efficient, Expansive, User-Defined Mutagenesis

Elad Firnberg, Marc Ostermeier

Deconstructing the Polymerase Chain Reaction: Understanding and Correcting Bias Associated with Primer Degeneracies and Primer-Template Mismatches

Stefan J. Green, Raghavee Venkatramanan, Ankur Naqib

A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data

Gregory J. Boggy, Peter J. Woolf

A Rapid and Simple Method for DNA Engineering Using Cycled Ligation Assembly

Theodore L. Roth, Ljiljana Milenkovic, Matthew P. Scott

Amplification of overlapping DNA amplicons in a single-tube multiplex PCR for targeted next-generation sequencing of BRCA1 and BRCA2

Desiree Schenk, Gang Song, Yue Ke, Zhaohui Wang

Seamless Insert-Plasmid Assembly at High Efficiency and Low Cost

Roger M. Benoit, Christian Ostermeier, [ ... ], Manfred Auer

Single-Molecule Three-Color FRET with Both Negligible Spectral Overlap and Long Observation Time

Sanghwa Lee, Jinwoo Lee, Sungchul Hohng

DNA Fragments Assembly Based on Nicking Enzyme System

Rui-Yan Wang, Zhen-Yu Shi, [ ... ], Guo-Qiang Chen

Identification and Differentiation of Verticillium Species and V. longisporum Lineages by Simplex and Multiplex PCR Assays

Patrik Inderbitzin, R. Michael Davis, Richard M. Bostock, Krishna V. Subbarao

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