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DNA construction

High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice

Jin Hee Kim, Sang-Rok Lee, [ ... ], Seok-Yong Choi

A Robust, Simple Genotyping-by-Sequencing (GBS) Approach for High Diversity Species

Robert J. Elshire, Jeffrey C. Glaubitz, [ ... ], Sharon E. Mitchell

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

Carola Engler, Romy Kandzia, Sylvestre Marillonnet

A Modular Cloning System for Standardized Assembly of Multigene Constructs

Ernst Weber, Carola Engler, [ ... ], Sylvestre Marillonnet

Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

Carola Engler, Ramona Gruetzner, Romy Kandzia, Sylvestre Marillonnet

Evaluation of Methods for the Extraction and Purification of DNA from the Human Microbiome

Sanqing Yuan, Dora B. Cohen, [ ... ], Larry J. Forney

A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells

Eric Campeau, Victoria E. Ruhl, [ ... ], Paul D. Kaufman

A One-Step Miniprep for the Isolation of Plasmid DNA and Lambda Phage Particles

George Lezin, Yasuhiro Kosaka, [ ... ], Luca Brunelli

Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways

Jiayuan Quan, Jingdong Tian

GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis

Athanasios Lampropoulos, Zoran Sutikovic, [ ... ], Joachim Forner

Cloning Should Be Simple: Escherichia coli DH5α-Mediated Assembly of Multiple DNA Fragments with Short End Homologies

Maxim Kostylev, Anne E. Otwell, Ruth E. Richardson, Yo Suzuki

Chromatin Immunoprecipitation (ChIP): Revisiting the Efficacy of Sample Preparation, Sonication, Quantification of Sheared DNA, and Analysis via PCR

Pamela D. Schoppee Bortz, Brian R. Wamhoff

Optimal Cloning of PCR Fragments by Homologous Recombination in Escherichia coli

Ana Paula Jacobus, Jeferson Gross

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