A. A fluorescent image stack rendered as volume data. The raw data set contained 159 z-direction optical sections. Each section is a 1024×1024 image. X-Y pixel size: 0.10×0.10 µm; z-step size: 0.20 µm. B. En face view of three virtual sections generated by resampling the stack in A along the Y-axis at positions schematically indicated by white lines in A.