On top of the membrane, a microfabricated PDMS flow chamber is clamped to control the media; b) Top: Sequence of images showing overlay of phase and YFP fluorescence of a colony of cells that carry the bud neck marker CDC10-YFP during exponential growth; Bottom : cell contours and parentage as retrieved by the annotation software; Right images show enlargments of the cell colony (top), bud neck position (middle), and cell contour and parentage (bottom); c) Growth curves of WT cells during a time-lapse experiment. Data using a standard agar setup (blue line) and the flow chamber (red line) were fit using an exponential (dashed line, discarding the first 100 minutes). The doubling time was respectively 77 and 78 minutes.