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Research Article

Robust Detection of Rare Species Using Environmental DNA: The Importance of Primer Specificity

  • Taylor M. Wilcox mail,

    taylor.wilcox@umontana.edu

    Affiliation: United States Department of Agriculture Forest Service, Rocky Mountain Research Station, Missoula, Montana, United States of America

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  • Kevin S. McKelvey,

    Affiliation: United States Department of Agriculture Forest Service, Rocky Mountain Research Station, Missoula, Montana, United States of America

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  • Michael K. Young,

    Affiliation: United States Department of Agriculture Forest Service, Rocky Mountain Research Station, Missoula, Montana, United States of America

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  • Stephen F. Jane,

    Affiliation: Department of Environmental Conservation, University of Massachusetts, Amherst, Massachusetts, United States of America

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  • Winsor H. Lowe,

    Affiliation: Division of Biological Sciences, University of Montana, Missoula, Montana, United States of America

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  • Andrew R. Whiteley,

    Affiliation: Department of Environmental Conservation, University of Massachusetts, Amherst, Massachusetts, United States of America

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  • Michael K. Schwartz

    Affiliation: United States Department of Agriculture Forest Service, Rocky Mountain Research Station, Missoula, Montana, United States of America

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  • Published: March 26, 2013
  • DOI: 10.1371/journal.pone.0059520

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