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Research Article

A Yeast Purification System for Human Translation Initiation Factors eIF2 and eIF2Bε and Their Use in the Diagnosis of CACH/VWM Disease

  • Rogerio A. de Almeida,

    Affiliation: Faculty of Life Sciences, The University of Manchester, Manchester, United Kingdom

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  • Anne Fogli,

    Affiliations: Laboratoire GReD (Génétique, Reproduction et Développement), Faculté de Médecine, INSERM U1103 CNRS 6293, Clermont-Ferrand, France, Université de Clermont, UFR Médecine, Clermont-Ferrand, France, Centre Hospitalier Universitaire de Clermont-Ferrand, Service de Biochimie Médicale et Biologie Moléculaire, Clermont-Ferrand, France

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  • Marina Gaillard,

    Affiliation: Laboratoire GReD (Génétique, Reproduction et Développement), Faculté de Médecine, INSERM U1103 CNRS 6293, Clermont-Ferrand, France

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  • Gert C. Scheper,

    Affiliation: Department of Pediatrics, VU University Medical Centre, Amsterdam, The Netherlands

    Current address: Crucell Holland BV, Leiden, The Netherlands

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  • Odile Boesflug-Tanguy,

    Affiliations: INSERM U676, Hopital Robert Debré, Paris, France, Université Paris Diderot, Sorbonne Cité, UMR U676, Paris, France, APHP, Hopital Robert Debré, Pediatric Neurology and Metabolic Diseases, Reference Center for Leukodystrophies, Paris, France

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  • Graham D. Pavitt mail

    graham.pavitt@manchester.ac.uk

    Affiliation: Faculty of Life Sciences, The University of Manchester, Manchester, United Kingdom

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  • Published: January 15, 2013
  • DOI: 10.1371/journal.pone.0053958

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