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Research Article

Optimized PCR Conditions and Increased shRNA Fold Representation Improve Reproducibility of Pooled shRNA Screens

  • Žaklina Strezoska,

    Affiliation: Molecular Biology, Thermo Fisher Scientific, Lafayette, Colorado, United States of America

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  • Abel Licon,

    Affiliation: Molecular Biology, Thermo Fisher Scientific, Lafayette, Colorado, United States of America

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  • Josh Haimes,

    Affiliation: Molecular Biology, Thermo Fisher Scientific, Lafayette, Colorado, United States of America

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  • Katie Jansen Spayd,

    Affiliation: Molecular Biology, Thermo Fisher Scientific, Lafayette, Colorado, United States of America

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  • Kruti M. Patel,

    Affiliation: Molecular Biology, Thermo Fisher Scientific, Lafayette, Colorado, United States of America

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  • Kevin Sullivan,

    Affiliation: Molecular Biology, Thermo Fisher Scientific, Lafayette, Colorado, United States of America

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  • Katarzyna Jastrzebski,

    Affiliation: Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia

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  • Kaylene J. Simpson,

    Affiliations: Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia, The Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, Victoria, Australia

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  • Devin Leake,

    Affiliation: Molecular Biology, Thermo Fisher Scientific, Lafayette, Colorado, United States of America

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  • Anja van Brabant Smith,

    Affiliation: Molecular Biology, Thermo Fisher Scientific, Lafayette, Colorado, United States of America

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  • Annaleen Vermeulen mail

    annleen.vermeulen@thermofisher.com

    Affiliation: Molecular Biology, Thermo Fisher Scientific, Lafayette, Colorado, United States of America

    X
  • Published: August 01, 2012
  • DOI: 10.1371/journal.pone.0042341

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