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Research Article

Automated Microinjection of Recombinant BCL-X into Mouse Zygotes Enhances Embryo Development

  • Xinyu Liu equal contributor,

    equal contributor Contributed equally to this work with: Xinyu Liu, Roxanne Fernandes

    Affiliation: Department of Mechanical and Industrial Engineering and Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada

    Current address: Department of Mechanical Engineering, McGill University, Montreal, Quebec, Canada

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  • Roxanne Fernandes equal contributor,

    equal contributor Contributed equally to this work with: Xinyu Liu, Roxanne Fernandes

    Affiliation: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada

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  • Marina Gertsenstein,

    Affiliation: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada

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  • Alagammal Perumalsamy,

    Affiliation: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada

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  • Ingrid Lai,

    Affiliation: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada

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  • Maggie Chi,

    Affiliation: Department of Obstetrics and Gynecology, Washington University in St. Louis, St. Louis, Missouri, United States of America

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  • Kelle H. Moley,

    Affiliation: Department of Obstetrics and Gynecology, Washington University in St. Louis, St. Louis, Missouri, United States of America

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  • Ellen Greenblatt,

    Affiliation: Department of Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada

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  • Igor Jurisica,

    Affiliation: Departments of Computer Science and Medical Biophysics, Ontario Cancer Institute and the Campbell Family Institute for Cancer Research, University Health Network, University of Toronto, Toronto, Ontario, Canada

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  • Robert F. Casper,

    Affiliations: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada, Department of Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada

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  • Yu Sun mail,

    sun@mie.utoronto.ca (YS); jurisicova@lunenfeld.ca (AJ)

    Affiliation: Department of Mechanical and Industrial Engineering and Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada

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  • Andrea Jurisicova mail

    sun@mie.utoronto.ca (YS); jurisicova@lunenfeld.ca (AJ)

    Affiliations: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada, Department of Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada

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  • Published: July 20, 2011
  • DOI: 10.1371/journal.pone.0021687

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