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Research Article

Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

  • Carola Engler equal contributor,

    equal contributor Contributed equally to this work with: Carola Engler, Ramona Gruetzner

    Affiliation: Icon Genetics GmbH, Biozentrum Halle, Halle, Germany

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  • Ramona Gruetzner equal contributor,

    equal contributor Contributed equally to this work with: Carola Engler, Ramona Gruetzner

    Affiliation: Icon Genetics GmbH, Biozentrum Halle, Halle, Germany

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  • Romy Kandzia,

    Affiliation: Icon Genetics GmbH, Biozentrum Halle, Halle, Germany

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  • Sylvestre Marillonnet mail

    marillonnet@icongenetics.de

    Affiliation: Icon Genetics GmbH, Biozentrum Halle, Halle, Germany

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  • Published: May 14, 2009
  • DOI: 10.1371/journal.pone.0005553

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