Physiological Normoxia and Absence of EGF Is Required for the Long-Term Propagation of Anterior Neural Precursors from Human Pluripotent Cells

Bilada Bilican; Matthew R. Livesey; Ghazal Haghi; Jing Qiu; Karen Burr; Rick Siller; Giles E. Hardingham; David J. A. Wyllie; Siddharthan Chandran

doi:10.1371/journal.pone.0085932

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Research Article

Physiological Normoxia and Absence of EGF Is Required for the Long-Term Propagation of Anterior Neural Precursors from Human Pluripotent Cells

Bilada Bilican ,

Contributed equally to this work with: Bilada Bilican, Matthew R. Livesey

Affiliations Euan MacDonald Centre for Motor Neurone Disease Research, University of Edinburgh, Edinburgh, United Kingdom, MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom, Centre for Neuroregeneration, University of Edinburgh, Edinburgh, United Kingdom
⨯
Matthew R. Livesey ,

Contributed equally to this work with: Bilada Bilican, Matthew R. Livesey

Affiliations Euan MacDonald Centre for Motor Neurone Disease Research, University of Edinburgh, Edinburgh, United Kingdom, Centre for Neuroregeneration, University of Edinburgh, Edinburgh, United Kingdom, Centre for Integrative Physiology, University of Edinburgh, Edinburgh, United Kingdom
⨯
Ghazal Haghi,

Affiliations Euan MacDonald Centre for Motor Neurone Disease Research, University of Edinburgh, Edinburgh, United Kingdom, Centre for Neuroregeneration, University of Edinburgh, Edinburgh, United Kingdom, Centre for Integrative Physiology, University of Edinburgh, Edinburgh, United Kingdom
⨯
Jing Qiu,

Affiliation Centre for Integrative Physiology, University of Edinburgh, Edinburgh, United Kingdom
⨯
Karen Burr,

Affiliations Euan MacDonald Centre for Motor Neurone Disease Research, University of Edinburgh, Edinburgh, United Kingdom, MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom, Centre for Neuroregeneration, University of Edinburgh, Edinburgh, United Kingdom
⨯
Rick Siller,

Affiliations MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom, Centre for Neuroregeneration, University of Edinburgh, Edinburgh, United Kingdom
⨯
Giles E. Hardingham,

Affiliation Centre for Integrative Physiology, University of Edinburgh, Edinburgh, United Kingdom
⨯
David J. A. Wyllie ,

* E-mail: dwyllie1@staffmail.ed.ac.uk (DJAW); siddharthan.chandran@ed.ac.uk (SC)

Affiliation Centre for Integrative Physiology, University of Edinburgh, Edinburgh, United Kingdom
⨯
Siddharthan Chandran

* E-mail: dwyllie1@staffmail.ed.ac.uk (DJAW); siddharthan.chandran@ed.ac.uk (SC)

Affiliations Euan MacDonald Centre for Motor Neurone Disease Research, University of Edinburgh, Edinburgh, United Kingdom, MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom, Centre for Neuroregeneration, University of Edinburgh, Edinburgh, United Kingdom
⨯

Physiological Normoxia and Absence of EGF Is Required for the Long-Term Propagation of Anterior Neural Precursors from Human Pluripotent Cells

Bilada Bilican,
Matthew R. Livesey,
Ghazal Haghi,
Jing Qiu,
Karen Burr,
Rick Siller,
Giles E. Hardingham,
David J. A. Wyllie,
Siddharthan Chandran

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Published: January 17, 2014
https://doi.org/10.1371/journal.pone.0085932

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Neuronal differentiation
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Developmental neuroscience
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Immunofluorescence
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Motor neurons
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