Ribosomal Binding Site Switching: An Effective Strategy for High-Throughput Cloning Constructions

Open Access

Peer-reviewed

Research Article

Yangbo Hu ,

Contributed equally to this work with: Yangbo Hu, Lipeng Feng

Affiliation Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
⨯
Lipeng Feng ,

Contributed equally to this work with: Yangbo Hu, Lipeng Feng

Affiliations Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China, Graduate University of the Chinese Academy of Sciences, Beijing, China
⨯
Yunlong Li,

Affiliations Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China, Graduate University of the Chinese Academy of Sciences, Beijing, China
⨯
Yong Zhang,

Affiliation Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
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Pei Lu,

Affiliation Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
⨯
Simon Rayner,

Affiliation Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
⨯
Shiyun Chen

* E-mail: sychen@wh.iov.cn

Affiliation Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
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Cloning
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Polymerase chain reaction
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Gene cloning
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Vector cloning
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Blunt-end ligation
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Plasmid construction
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DNA polymerase
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Gene expression
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