Comprehensive Multiplex One-Step Real-Time TaqMan qRT-PCR Assays for Detection and Quantification of Hemorrhagic Fever Viruses

Zheng Pang; Aqian Li; Jiandong Li; Jing Qu; Chengcheng He; Shuo Zhang; Chuan Li; Quanfu Zhang; Mifang Liang; Dexin Li

doi:10.1371/journal.pone.0095635

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Open Access

Peer-reviewed

Research Article

Comprehensive Multiplex One-Step Real-Time TaqMan qRT-PCR Assays for Detection and Quantification of Hemorrhagic Fever Viruses

Zheng Pang ,

Contributed equally to this work with: Zheng Pang, Aqian Li

Affiliation Key Laboratory of Medical Virology, NHFPC, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
⨯
Aqian Li ,

Contributed equally to this work with: Zheng Pang, Aqian Li

Affiliation Key Laboratory of Medical Virology, NHFPC, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
⨯
Jiandong Li,

Affiliation Key Laboratory of Medical Virology, NHFPC, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
⨯
Jing Qu,

Affiliation Key Laboratory of Medical Virology, NHFPC, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
⨯
Chengcheng He,

Affiliation Key Laboratory of Medical Virology, NHFPC, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
⨯
Shuo Zhang,

Affiliation Key Laboratory of Medical Virology, NHFPC, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
⨯
Chuan Li,

Affiliation Key Laboratory of Medical Virology, NHFPC, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
⨯
Quanfu Zhang,

Affiliation Key Laboratory of Medical Virology, NHFPC, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
⨯
Mifang Liang,

Affiliation Key Laboratory of Medical Virology, NHFPC, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
⨯
Dexin Li

* E-mail: lidx@chinacdc.cn

Affiliation Key Laboratory of Medical Virology, NHFPC, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
⨯

Comprehensive Multiplex One-Step Real-Time TaqMan qRT-PCR Assays for Detection and Quantification of Hemorrhagic Fever Viruses

Zheng Pang,
Aqian Li,
Jiandong Li,
Jing Qu,
Chengcheng He,
Shuo Zhang,
Chuan Li,
Quanfu Zhang,
Mifang Liang,
Dexin Li

x

Published: April 21, 2014
https://doi.org/10.1371/journal.pone.0095635

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Dengue virus
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Hemorrhagic fever viruses
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Rift Valley fever virus
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Reverse transcriptase-polymerase chain reaction
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RNA extraction
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Viral hemorrhagic fevers
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