Direct Staining with Major Histocompatibility Complex Class II Dextramers Permits Detection of Antigen-Specific, Autoreactive CD4 T Cells In Situ

Chandirasegaran Massilamany; Arunakumar Gangaplara; Ting Jia; Christian Elowsky; Guobin Kang; Jean-Jack Riethoven; Qingsheng Li; You Zhou; Jay Reddy

doi:10.1371/journal.pone.0087519

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Peer-reviewed

Research Article

Direct Staining with Major Histocompatibility Complex Class II Dextramers Permits Detection of Antigen-Specific, Autoreactive CD4 T Cells In Situ

Chandirasegaran Massilamany,

Affiliation School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America
⨯
Arunakumar Gangaplara ,

Contributed equally to this work with: Arunakumar Gangaplara, Ting Jia

Affiliation School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America
⨯
Ting Jia ,

Contributed equally to this work with: Arunakumar Gangaplara, Ting Jia

Affiliation School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America
⨯
Christian Elowsky,

Affiliation Center for Biotechnology, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America
⨯
Guobin Kang,

Affiliation Nebraska Center for Virology and School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America
⨯
Jean-Jack Riethoven,

Affiliation Center for Biotechnology, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America
⨯
Qingsheng Li,

Affiliation Nebraska Center for Virology and School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America
⨯
You Zhou,

Affiliation Center for Biotechnology, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America
⨯
Jay Reddy

* E-mail: nreddy2@unl.edu

Affiliation School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America
⨯

Direct Staining with Major Histocompatibility Complex Class II Dextramers Permits Detection of Antigen-Specific, Autoreactive CD4 T Cells In Situ

Chandirasegaran Massilamany,
Arunakumar Gangaplara,
Ting Jia,
Christian Elowsky,
Guobin Kang,
Jean-Jack Riethoven,
Qingsheng Li,
You Zhou,
Jay Reddy

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Published: January 27, 2014
https://doi.org/10.1371/journal.pone.0087519

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T cells
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Cell staining
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Major histocompatibility complex
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T helper cells
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Heart
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Myosins
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Confocal laser microscopy
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Inflammation
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