AAscan, PCRdesign and MutantChecker: A Suite of Programs for Primer Design and Sequence Analysis for High-Throughput Scanning Mutagenesis

Dawei Sun; Martin K. Ostermaier; Franziska M. Heydenreich; Daniel Mayer; Rolf Jaussi; Joerg Standfuss; Dmitry B. Veprintsev

doi:10.1371/journal.pone.0078878

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Peer-reviewed

Research Article

AAscan, PCRdesign and MutantChecker: A Suite of Programs for Primer Design and Sequence Analysis for High-Throughput Scanning Mutagenesis

Dawei Sun ,

Contributed equally to this work with: Dawei Sun, Martin K. Ostermaier, Franziska M. Heydenreich

Affiliation Laboratory of Biomolecular Research, Paul Scherrer Institut, Villigen, Switzerland and Department of Biology, ETH Zurich, Zurich, Switzerland
⨯
Martin K. Ostermaier ,

Contributed equally to this work with: Dawei Sun, Martin K. Ostermaier, Franziska M. Heydenreich

Affiliation Laboratory of Biomolecular Research, Paul Scherrer Institut, Villigen, Switzerland and Department of Biology, ETH Zurich, Zurich, Switzerland
⨯
Franziska M. Heydenreich ,

Contributed equally to this work with: Dawei Sun, Martin K. Ostermaier, Franziska M. Heydenreich

Affiliation Laboratory of Biomolecular Research, Paul Scherrer Institut, Villigen, Switzerland and Department of Biology, ETH Zurich, Zurich, Switzerland
⨯
Daniel Mayer,

Affiliation Laboratory of Biomolecular Research, Paul Scherrer Institut, Villigen, Switzerland and Department of Biology, ETH Zurich, Zurich, Switzerland
⨯
Rolf Jaussi,

Affiliation Laboratory of Biomolecular Research, Paul Scherrer Institut, Villigen, Switzerland and Department of Biology, ETH Zurich, Zurich, Switzerland
⨯
Joerg Standfuss,

Affiliation Laboratory of Biomolecular Research, Paul Scherrer Institut, Villigen, Switzerland and Department of Biology, ETH Zurich, Zurich, Switzerland
⨯
Dmitry B. Veprintsev

* E-mail: dmitry.veprintsev@psi.ch

Affiliation Laboratory of Biomolecular Research, Paul Scherrer Institut, Villigen, Switzerland and Department of Biology, ETH Zurich, Zurich, Switzerland
⨯

AAscan, PCRdesign and MutantChecker: A Suite of Programs for Primer Design and Sequence Analysis for High-Throughput Scanning Mutagenesis

Dawei Sun,
Martin K. Ostermaier,
Franziska M. Heydenreich,
Daniel Mayer,
Rolf Jaussi,
Joerg Standfuss,
Dmitry B. Veprintsev

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Published: October 30, 2013
https://doi.org/10.1371/journal.pone.0078878

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Polymerase chain reaction
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Mutagenesis
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Computer software
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Sequence alignment
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Vector cloning
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