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Research Article

The Applicability of TaqMan-Based Quantitative Real-Time PCR Assays for Detecting and Enumerating Cryptosporidium spp. Oocysts in the Environment

  • Sarah E. Staggs,

    Affiliation: National Exposure Research Laboratory, US Environmental Protection Agency, Cincinnati, Ohio, United States of America

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  • Erin M. Beckman,

    Affiliation: Dynamac Corporation, Cincinnati, Ohio, United States of America

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  • Scott P. Keely,

    Affiliation: National Exposure Research Laboratory, US Environmental Protection Agency, Cincinnati, Ohio, United States of America

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  • Reena Mackwan,

    Affiliation: Dynamac Corporation, Cincinnati, Ohio, United States of America

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  • Michael W. Ware,

    Affiliation: National Exposure Research Laboratory, US Environmental Protection Agency, Cincinnati, Ohio, United States of America

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  • Alan P. Moyer,

    Affiliation: Department of Biological Sciences, McMicken School of Arts and Sciences, University of Cincinnati, Cincinnati, Ohio, United States of America

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  • James A. Ferretti,

    Affiliation: Region 2, US Environmental Protection Agency, Edison, New Jersey, United States of America

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  • Abu Sayed,

    Affiliation: Dynamac Corporation, Cincinnati, Ohio, United States of America

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  • Lihua Xiao,

    Affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America

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  • Eric N. Villegas mail

    villegas.eric@epa.gov

    Affiliations: National Exposure Research Laboratory, US Environmental Protection Agency, Cincinnati, Ohio, United States of America, Department of Biological Sciences, McMicken School of Arts and Sciences, University of Cincinnati, Cincinnati, Ohio, United States of America

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  • Published: June 21, 2013
  • DOI: 10.1371/journal.pone.0066562

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