A Versatile Method to Design Stem-Loop Primer-Based Quantitative PCR Assays for Detecting Small Regulatory RNA Molecules

• Zsolt Czimmerer,

  Affiliation Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, University of Debrecen Medical and Health Science Center, Debrecen, Hungary

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• Julianna Hulvely,

  Affiliation Astrid Research Ltd, Debrecen, Hungary

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• Zoltan Simandi,

  Affiliation Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, University of Debrecen Medical and Health Science Center, Debrecen, Hungary

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• Eva Varallyay,

  Affiliation Plant Developmental Biology Group, Agricultural Biotechnology Center, Gödöllő, Hungary

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• Zoltan Havelda,

  Affiliation Plant Developmental Biology Group, Agricultural Biotechnology Center, Gödöllő, Hungary

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• Erzsebet Szabo,

  Affiliation Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, University of Debrecen Medical and Health Science Center, Debrecen, Hungary

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• Attila Varga,

  Affiliation Astrid Research Ltd, Debrecen, Hungary

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• Balazs Dezso,

  Affiliation Institute of Pathology, University of Debrecen Medical and Health Science Center, Debrecen, Hungary

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• Maria Balogh,

  Affiliation Center for Clinical Genomics and Personalized Medicine, Research Center for Molecular Medicine, University of Debrecen Medical and Health Science Center, Debrecen, Hungary

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• Attila Horvath,

  Affiliations Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, University of Debrecen Medical and Health Science Center, Debrecen, Hungary, Astrid Research Ltd, Debrecen, Hungary

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• Balint Domokos,

  Affiliation Astrid Research Ltd, Debrecen, Hungary

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• Zsolt Torok,

  Affiliations Astrid Research Ltd, Debrecen, Hungary, Department of Computer Graphics and Image Processing, University of Debrecen Centre of Arts, Humanities and Sciences, Debrecen, Hungary

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• Laszlo Nagy,

  Affiliations Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, University of Debrecen Medical and Health Science Center, Debrecen, Hungary, MTA-DE “Lendulet” Immunogenomics Research Group, University of Debrecen, Medical and Health Science Center, Debrecen, Hungary

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• Balint L. Balint

  * E-mail: lbalint@med.unideb.hu

  Affiliations Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, University of Debrecen Medical and Health Science Center, Debrecen, Hungary, Center for Clinical Genomics and Personalized Medicine, Research Center for Molecular Medicine, University of Debrecen Medical and Health Science Center, Debrecen, Hungary, UD-Genomed Ltd, Debrecen, Hungary

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