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Quantification of Reverse Transcriptase Activity by Real-Time PCR as a Fast and Accurate Method for Titration of HIV, Lenti- and Retroviral Vectors

  • Jolien Vermeire,

    Affiliation Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium

  • Evelien Naessens,

    Affiliation Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium

  • Hanne Vanderstraeten,

    Affiliation Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium

  • Alessia Landi,

    Affiliation Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium

  • Veronica Iannucci,

    Affiliation Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium

  • Anouk Van Nuffel,

    Affiliation Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium

  • Tom Taghon,

    Affiliation Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium

  • Massimo Pizzato,

    Affiliation Centre for Integrative Biology (CIBIO), University of Trento, Trento, Italy

  • Bruno Verhasselt

    bruno.verhasselt@ugent.be

    Affiliation Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium

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