Evaluating Methods for Isolating Total RNA and Predicting the Success of Sequencing Phylogenetically Diverse Plant Transcriptomes

• Marc T. J. Johnson ,

  Contributed equally to this work with: Marc T. J. Johnson, Eric J. Carpenter, Zhijian Tian

  * E-mail: marc.johnson@utoronto.ca (MJ); gane@ualberta.ca (GKSW)

  Affiliation Department of Biology, University of Toronto at Mississauga, Mississauga, Ontario, Canada

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• Eric J. Carpenter ,

  Contributed equally to this work with: Marc T. J. Johnson, Eric J. Carpenter, Zhijian Tian

  Affiliation Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada

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• Zhijian Tian ,

  Contributed equally to this work with: Marc T. J. Johnson, Eric J. Carpenter, Zhijian Tian

  Affiliation BGI-Shenzhen, Bei Shan Industrial Zone, Yantian District, Shenzhen, China

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• Richard Bruskiewich,

  Current address: Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada

  Affiliation International Rice Research Institute, Metro Manila, Philippines

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• Jason N. Burris,

  Affiliation Department of Plant Sciences, University of Tennessee, Knoxville, Tennessee, United States of America

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• Charlotte T. Carrigan,

  Affiliation Department of Plant Biology, University of Georgia, Athens, Georgia, United States of America

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• Mark W. Chase,

  Affiliation Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey, United Kingdom

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• Neil D. Clarke,

  Affiliation Genome Institute of Singapore, Singapore, Singapore

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• Sarah Covshoff,

  Affiliation Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom

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• Claude W. dePamphilis,

  Affiliation Department of Biology and Intercollege Graduate Program in Plant Biology, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania, United States of America

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• Patrick P. Edger,

  Affiliation Division of Biological Sciences, University of Missouri, Columbia, Missouri, United States of America

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• Falicia Goh,

  Affiliation Genome Institute of Singapore, Singapore, Singapore

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• Sean Graham,

  Affiliation Department of Botany and UBC Botanical Garden, University of British Columbia, Vancouver, British Columbia, Canada

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• Stephan Greiner,

  Affiliation Max Planck Institute for Molecular Plant Physiology, Wissenschaftspark Golm, Am Mühlenberg 1, Potsdam-Golm, Germany

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• Julian M. Hibberd,

  Affiliation Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom

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• Ingrid Jordon-Thaden,

  Affiliations Department of Biology, University of Florida, Gainesville, Florida, United States of America, Florida Museum of Natural History, University of Florida, Gainesville, Florida, United States of America

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• Toni M. Kutchan,

  Affiliation Donald Danforth Plant Science Center, St. Louis, Missouri, United States of America

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• James Leebens-Mack,

  Affiliation Department of Plant Biology, University of Georgia, Athens, Georgia, United States of America

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• Michael Melkonian,

  Affiliation Department of Botany, Cologne Biocenter, University of Cologne, Cologne, Germany

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• Nicholas Miles,

  Affiliations Department of Biology, University of Florida, Gainesville, Florida, United States of America, Florida Museum of Natural History, University of Florida, Gainesville, Florida, United States of America

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• Henrietta Myburg,

  Affiliation Department of Plant Biology, North Carolina State University, Raleigh, North Carolina, United States of America

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• Jordan Patterson,

  Affiliation Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada

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• J. Chris Pires,

  Affiliation Division of Biological Sciences, University of Missouri, Columbia, Missouri, United States of America

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• Paula Ralph,

  Affiliation Department of Biology and Intercollege Graduate Program in Plant Biology, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania, United States of America

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• Megan Rolf,

  Affiliation Donald Danforth Plant Science Center, St. Louis, Missouri, United States of America

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• Rowan F. Sage,

  Affiliation Department of Ecology and Evolutionary Biology, University of Toronto, Toronto, Ontario, Canada

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• Douglas Soltis,

  Affiliation Department of Biology, University of Florida, Gainesville, Florida, United States of America

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• Pamela Soltis,

  Affiliation Florida Museum of Natural History, University of Florida, Gainesville, Florida, United States of America

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• Dennis Stevenson,

  Affiliation New York Botanical Garden, Bronx, New York, United States of America

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• C. Neal Stewart Jr,

  Affiliation Department of Plant Sciences, University of Tennessee, Knoxville, Tennessee, United States of America

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• Barbara Surek,

  Affiliation Department of Botany, Cologne Biocenter, University of Cologne, Cologne, Germany

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• Christina J. M. Thomsen,

  Affiliation Department of Biology, University of Toronto at Mississauga, Mississauga, Ontario, Canada

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• Juan Carlos Villarreal,

  Affiliation Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, Connecticut, United States of America

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• Xiaolei Wu,

  Affiliation BGI-Shenzhen, Bei Shan Industrial Zone, Yantian District, Shenzhen, China

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• Yong Zhang,

  Affiliation BGI-Shenzhen, Bei Shan Industrial Zone, Yantian District, Shenzhen, China

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• Michael K. Deyholos,

  Affiliation Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada

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•  [ ... ],
• Gane Ka-Shu Wong

  * E-mail: marc.johnson@utoronto.ca (MJ); gane@ualberta.ca (GKSW)

  Affiliations Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada, BGI-Shenzhen, Bei Shan Industrial Zone, Yantian District, Shenzhen, China, Department of Medicine, University of Alberta, Edmonton, Alberta, Canada

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