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Improving Lambda Red Genome Engineering in Escherichia coli via Rational Removal of Endogenous Nucleases

  • Joshua A. Mosberg ,

    Contributed equally to this work with: Joshua A. Mosberg, Christopher J. Gregg, Marc J. Lajoie

    Affiliation Program in Chemical Biology, Harvard University, Cambridge, Massachusetts, United States of America

  • Christopher J. Gregg ,

    Contributed equally to this work with: Joshua A. Mosberg, Christopher J. Gregg, Marc J. Lajoie

    Affiliation Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America

  • Marc J. Lajoie ,

    Contributed equally to this work with: Joshua A. Mosberg, Christopher J. Gregg, Marc J. Lajoie

    Affiliation Program in Chemical Biology, Harvard University, Cambridge, Massachusetts, United States of America

  • Harris H. Wang,

    Affiliations Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, United States of America, Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, United States of America

  • George M. Church

    gchurch@genetics.med.harvard.edu

    Affiliation Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America

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