Groundtruthing Next-Gen Sequencing for Microbial Ecology–Biases and Errors in Community Structure Estimates from PCR Amplicon Pyrosequencing

• Charles K. Lee ,

  Contributed equally to this work with: Charles K. Lee, Craig W. Herbold

  Affiliation Department of Biological Sciences, University of Waikato, Hamilton, New Zealand

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• Craig W. Herbold ,

  Contributed equally to this work with: Charles K. Lee, Craig W. Herbold

  Affiliation Department of Biological Sciences, University of Waikato, Hamilton, New Zealand

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• Shawn W. Polson,

  Affiliations Center for Bioinformatics and Computational Biology, Delaware Biotechnology Institute, University of Delaware, Newark, Delaware, United States of America, Department of Computer and Information Sciences, University of Delaware, Newark, Delaware, United States of America, Department of Biological Sciences, University of Delaware, Newark, Delaware, United States of America

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• K. Eric Wommack,

  Affiliations Department of Biological Sciences, University of Delaware, Newark, Delaware, United States of America, Department of Plant and Soil Sciences, University of Delaware, Newark, Delaware, United States of America, College of Earth, Ocean, and Environment, University of Delaware, Lewes, Delaware, United States of America

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• Shannon J. Williamson,

  Affiliation J. Craig Venter Institute, San Diego, California, United States of America

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• Ian R. McDonald,

  Affiliation Department of Biological Sciences, University of Waikato, Hamilton, New Zealand

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• S. Craig Cary

  * E-mail: caryc@waikato.ac.nz

  Affiliations Department of Biological Sciences, University of Waikato, Hamilton, New Zealand, College of Earth, Ocean, and Environment, University of Delaware, Lewes, Delaware, United States of America

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