An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching

• Michele L. Markwardt,

  Affiliation Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America

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• Gert-Jan Kremers,

  Current address: Department of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands

  Affiliation Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, United States of America

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• Catherine A. Kraft,

  Affiliation Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America

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• Krishanu Ray,

  Affiliation Center for Fluorescence Spectroscopy and the Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America

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• Paula J. C. Cranfill,

  Affiliation National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, Tallahassee, Florida, United States of America

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• Korey A. Wilson,

  Affiliation National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, Tallahassee, Florida, United States of America

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• Richard N. Day,

  Affiliation Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, Indiana, United States of America

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• Rebekka M. Wachter,

  Affiliation Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona United States of America

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• Michael W. Davidson,

  Affiliation National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, Tallahassee, Florida, United States of America

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• Megan A. Rizzo

  * E-mail: mrizz001@umaryland.edu

  Affiliation Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America

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