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Research Article

Resurrection of a Bull by Cloning from Organs Frozen without Cryoprotectant in a −80°C Freezer for a Decade

  • Yoichiro Hoshino,

    Affiliations: Gifu Prefectural Livestock Research Institute, Takayama, Gifu, Japan, Wakayama Industry Promotion Foundation, Wakayama, Japan

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  • Noboru Hayashi,

    Affiliations: Gifu Prefectural Livestock Research Institute, Takayama, Gifu, Japan, Department of Genetic Engineering, Kinki University, Kinokawa Wakayama, Japan, Wakayama Industry Promotion Foundation, Wakayama, Japan

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  • Shunji Taniguchi,

    Affiliations: Department of Genetic Engineering, Kinki University, Kinokawa Wakayama, Japan, Wakayama Industry Promotion Foundation, Wakayama, Japan

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  • Naohiko Kobayashi,

    Affiliations: Gifu Prefectural Livestock Research Institute, Takayama, Gifu, Japan, Department of Genetic Engineering, Kinki University, Kinokawa Wakayama, Japan, Wakayama Industry Promotion Foundation, Wakayama, Japan

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  • Kenji Sakai,

    Affiliations: Gifu Prefectural Livestock Research Institute, Takayama, Gifu, Japan, Department of Genetic Engineering, Kinki University, Kinokawa Wakayama, Japan

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  • Tsuyoshi Otani,

    Affiliations: Gifu Prefectural Livestock Research Institute, Takayama, Gifu, Japan, Department of Genetic Engineering, Kinki University, Kinokawa Wakayama, Japan

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  • Akira Iritani,

    Affiliations: Department of Genetic Engineering, Kinki University, Kinokawa Wakayama, Japan, Wakayama Industry Promotion Foundation, Wakayama, Japan

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  • Kazuhiro Saeki mail

    saeki@waka.kindai.ac.jp

    Affiliations: Department of Genetic Engineering, Kinki University, Kinokawa Wakayama, Japan, Wakayama Industry Promotion Foundation, Wakayama, Japan

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  • Published: January 08, 2009
  • DOI: 10.1371/journal.pone.0004142

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