http://orcid.org/0000-0002-0933-5785
Figures
Abstract
The reproductive ground plan hypothesis proposes that gene networks regulating foraging behavior and reproductive female physiology in social insects emerged from ancestral gene and endocrine factor networks. Expression of storage proteins such as vitellogenins and hexamerins is an example of this co-option. Hexamerins, through their role modulating juvenile hormone availability, are involved in caste determination in termites. The genome of the fire ant (Solenopsis invicta) encodes four hexamerin genes, hexamerin-like (LOC105192919, hereafter called hexamerin 1), hexamerin (LOC105204474, hereafter called hexamerin 2), arylphorin subunit alpha-like, and arylphorin subunit beta. In this study, a phylogenetic analysis of the S. invicta hexamerins determined that each predicted protein clustered with one of the orthologous Apis mellifera hexamerins. Gene expression analyses by RT-qPCR revealed differential expression of the hexamerins between queens and workers, and between specific task-allocated workers (nurses and foragers). Queens and nurses had significantly higher expression of all genes when compared to foragers. Hexamerin 1 was expressed at higher levels in queens, while hexamerin 2 and arylphorin subunit beta were expressed at significantly higher levels in nurses. Arylphorin subunit alpha-like showed no significant difference in expression between virgin queens and nurses. Additionally, we analyzed the relationship between the expression of hexamerin genes and S-hydroprene, a juvenile hormone analog. Significant changes in hexamerin expression were recorded in nurses, virgin queens, and foragers 12 h after application of the analog. Hexamerin 1 and arylphorin subunit alpha-like expression were significantly lower after analog application in virgin queens. In foragers, hexamerin 2 and arylphorin subunit beta were significantly lower after analog application, while in nurses expression of all genes were significantly lower after analog application. Our results suggest that in S. invicta hexamerin genes could be associated with reproductive division of labor and task-allocation of workers.
Citation: Hawkings C, Calkins TL, Pietrantonio PV, Tamborindeguy C (2019) Caste-based differential transcriptional expression of hexamerins in response to a juvenile hormone analog in the red imported fire ant (Solenopsis invicta). PLoS ONE 14(5): e0216800. https://doi.org/10.1371/journal.pone.0216800
Editor: Olena Riabinina, Durham University, UNITED KINGDOM
Received: November 28, 2018; Accepted: April 29, 2019; Published: May 20, 2019
Copyright: © 2019 Hawkings et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the manuscript and its Supporting Information files.
Funding: Research was supported by competitive funds to CT and PVP from the Texas Invasive Ant Research and Management program from Texas A&M AgriLife Research and the National Science Foundation Integrative Organismal Systems award number 1257837 (2013–2017) to PVP and CT. This work is/was supported by the USDA National Institute of Food and Agriculture, Hatch project Accession Numbers 1015773 (CT), and 1002279 (PVP). CT and PVP programs are supported by NIFA. The open access publishing fees for this article have been covered by the Texas A&M University Online Access to Knowledge (OAK) Fund, supported by the University Libraries and the Office of the Vice President for Research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Introduction
The reproductive ground plan hypothesis proposes that the gene networks that regulate foraging behavior and reproductive female physiology in social insects emerged from ancestral networks of genes and endocrine factors [1,2,3]. Examples of this co-option of gene networks to regulate division of labor have been shown in several social hymenopteran species [4,5,6,7]. One of the genes recognized for its co-optive role is vitellogenin (Vg), which encodes a soluble yolk protein precursor involved in the production of eggs in oviparous species. In social insects, Vg expression in the queen is linked to its conventional role in reproduction, while in the workers it is linked to social organization, task allocation and age polyethism [8,9,10,11,12]. In Apis mellifera, Vg levels decrease as the workers transition from nursing to foraging tasks [8], and silencing of the Vg gene in nurses resulted in precocious foraging [13]. Similar studies conducted in several ant species have also identified a potential role of Vg in task allocation and colony organization based on the patterns of expression in task allocated insects or in response to changes in social context. For instance, Pogonomyrmex barbatus nurses express high levels of Vg transcript which decrease as the individual transitions into foraging tasks [14]. Similarly, in Ectatomma tuberculatum, production of Vg is related to the worker age: workers produce high levels of Vg at the beginning of their adult life when they conduct tasks associated with the brood inside the nest, while older workers which perform tasks outside the nest do not produce Vg [15].
In insects, juvenile hormone (JH) is a developmental hormone during the immature stages, and it can play a gonadotropic role promoting reproduction in adults [16]. The behavioral transition of workers, and the dominance position and reproductive status of hymenopteran queens have also been linked to JH titers [17,18,19,20,21,22], suggesting that JH may play a role in the expression of proteins that will change the behavior or task of an individual [22]. The role of JH in social insect age polyethism has been extensively studied in A. mellifera [18,23,24]. In this species, JH level is typically low in nurses, and increases as the workers transition into performing foraging tasks. JH is also involved in age polyethism in ants. For example, Pogonomyrmex californicus, Myrmicaria eumenoides and Harpegnathos saltator foragers have higher JH titers than the workers performing in-nest tasks [25,26,27]. Furthermore, in some ant species, a correlation between JH titer and Vg was identified. For example, Vg titer was down-regulated in E. tuberculatum sterile workers following topical application of JH [21].
In addition to Vg, hexamerins, a second family of storage proteins, have been also associated with social organization. Hexamerins, also known as larval storage proteins, are synthesized in the fat body, and are secreted and accumulate in the hemolymph of larvae. Later in development, they are reabsorbed into the fat body, broken down, and incorporated into adult tissues during the pupal molt [28]. In termites, hexamerins are involved in the regulation of caste determination because silencing of hexamerins results in a higher proportion of soldiers [29,30]. This latter function may be through an interaction of hexamerins with JH signaling [31]. In Polistes metricus, a social wasp, insects that will emerge to become gynes have higher Hexamerin 1 protein levels compared to those that will become workers [32]. Furthermore, several studies reported differences in hexamerin expression between castes of social Hymenoptera [33,34,35,36]. Specifically, in A. mellifera, two hexamerin genes are expressed in the adult fat body in a caste- and sex-specific manner, with higher expression in workers than in queens [37]. In ants, these proteins accumulate during the alate virgin period of the adult queen life stage and may serve as amino acid storage [38]. In some species, this storage is critical to the claustral period of colony formation; hexamerins allow queens to produce the first generation of workers without having to leave the nest to forage [38,39].
Investigating the expression and function of storage proteins in Solenopsis invicta is of particular interest because this species displays extreme reproductive division of labor between two basic caste forms. The worker caste is composed of polymorphic sterile females responsible for the tasks that support the growth and maintenance of the colony [40], while the reproductive adults are queen(s) and drones, which are responsible for producing the offspring. In a previous study, we investigated the expression of Vgs in the worker caste of S. invicta and we identified differences in the expression of Vg1 among workers performing different tasks [10]. Further, in contrast to worker bees, none of the four Vg genes were regulated in workers by topical application of the JH-analog S-hydroprene [10]. In a different study, we have shown that when comparing brain transcriptomes of alate virgin and dealate mated queens, the expression of two hexamerins, hexamerin-like (LOC105192919, hereafter called hexamerin 1) and arylphorin subunit alpha-like (LOC105192898), is significantly reduced in brains of mated queens [41]. In the same transcriptome analysis, no differences in expression were observed for the other two hexamerin genes, hexamerin (LOC105204474, hereafter called hexamerin 2) and arylphorin subunit beta (LOC105192897). Moreover, the topical application of the JH analog S-hydroprene resulted in decreased expression of hexamerin 1 in whole bodies of virgin queens [41]. However, the effect of S-hydroprene on the expression of the other hexamerin genes in queens and on the expression of all hexamerins in adult workers was not determined.
Despite the knowledge about the involvement of storage proteins in caste determination in social insects, the expression and roles of hexamerin genes in S. invicta has not yet been investigated. In particular, knowledge gaps exist regarding the relationship between hexamerin expression and JH, a key regulator of social organization. In this species, while JH has retained its gonadotropic role in the queen [42], it is still unknown if JH is involved in worker age polyethism. Similarly, whether hexamerin expression is regulated by JH in workers or the interplay of JH and hexamerins in the regulation of worker tasks in S. invicta has not been investigated. Thus, in this study, we evaluated the expression of the four hexamerin genes in S. invicta workers and queens, and investigated whether those genes were regulated by a JH analog in a caste- or task-specific manner.
Materials and methods
Insect colonies
Polygyne colonies of S. invicta were collected in College Station, Brazos County, Texas, from July to September of 2017 and maintained in the Department of Entomology at Texas A&M University, College Station, Texas, as laboratory colonies in plastic containers (27 x 40 x 9 cm). The inside of these containers was coated with Fluon to prevent ant escape (Insect-a-slip, Bioquip products, CA). The colonies were kept at 27± 2°C in a 12:12 hour dark-light photoperiod. Colonies were provided with half-filled water tubes, capped with cotton as a damp nesting area, and fed daily with both 20% honey solution and crickets (Acheta domestica) as a protein source. Water was provided ad libitum. Colonies contained mated queens (dealate), virgin queens (alate), drones, polymorphic workers and brood (eggs, larvae and pupae). Mated queens for gene expression analyses were collected immediately after a mating flight in College Station, Texas in May 2018. These mated queens were kept as previously described in a container with half-filled water tubes, capped with cotton as a damp nesting area for approximately one week after the first batch of eggs were laid.
Phylogenetic analysis
Apis mellifera, Nasonia vitripennis, Camponotus floridanus, Harpegnathos saltator, Acromyrmex echinatior, Atta colombica, and Lasius niger hexamerin proteins were identified using Blastp searches or mining the respective genomes. The protein sequences were downloaded in fasta format and aligned using MAFFT v.7 [43] using the amino acid alignment default parameters. The alignments were visually assessed using Mesquite v. 3.03 [44] and the sequences were manually trimmed. The phylogenetic tree was reconstructed by Bayesian inference (Mr. Bayes v 3.2.3) [45] on the CIPRES supercomputer [46] with four runs of a mixed amino acid model for 1,000,000 generations, and a 10% burnin. Convergence of the runs were assessed in Tracer 1.6. The consensus tree was visualized in FigTree version 1.4.2 (http://tree.bio.ed.ac.uk/software/figtree/) and rooted using the termite Reticulitermes flavipes hexamerin proteins as the outgroup.
Classification and selection of ants
Virgin queens (alate) were selected based on the presence of wings. Mated queens (dealate) were selected if they had laid eggs. The workers were classified based upon their head width [47], and for this study only medium-size workers were used (head width between 0.73 and 0.92 mm), which are the intermediate size workers within the colony. Foraging workers were individuals in the foraging arena who were actively interacting with food resources outside of the nest. Nurses were individuals inside the nest that were actively interacting with the brood (any immature developmental stage). All individuals were of unknown age.
Expression analysis of hexamerins in workers and queens
Gene expression analyses were conducted using pools of ten individual workers or of five individual queens to obtain sufficient RNA. Individuals in each pool were from the same colony; each pool was obtained from a different colony. Workers were pooled according to the task performed (nursing or foraging). Pooled alate virgin and mated queens were of unknown age. Pools were flash-frozen in liquid nitrogen upon collection and stored at -80°C until gene expression analysis.
Insects were ground in liquid nitrogen to a fine powder using a mortar and pestle. Total RNA extractions were conducted using Trizol reagent (Invitrogen, Carlsbad, CA) following the manufacturer’s protocol. Purification of RNA was performed using the Direct-zol microprep kit (Zymo Research, Irvine, CA) for clean-up of the samples. RNA was resuspended in 20 μl of nuclease-free water. Genomic DNA was eliminated using the Turbo DNAse kit (Ambion, Waltham, MA) following the manufacturer’s protocol. Total RNA was quantified using the Infinite 200 PRO NanoQuant (Tecan, Männedorf, Switzerland), and its integrity was assessed using gel electrophoresis, visualized on a 2% agarose gel stained with ethidium bromide.
For gene expression analyses, RT-qPCR reactions were performed using the SensiFAST SYBR Hi-ROX One Step kit (Bioline, Taunton, MA) following the manufacturer’s protocol. All reactions contained 50 ng of total RNA, 250 nM of forward and 250 nM of reverse primers [41], 2X SensiFAST SYBR Hi-ROX One-Step Mix, reverse transcriptase, and RiboSafe RNAse inhibitor; the volume was adjusted with nuclease-free water to 10 μl. Primers are listed in S1 Table. The thermocycler parameters were set to 45°C for 10 min, then 95°C for 2 min and 40 cycles of 95°C for 5 sec and 60°C for 30 sec. RT-qPCR was performed using the Applied Biosystems QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA) according to manufacturer’s instructions. Each reaction was performed in duplicate, and negative controls for each reaction were included. Primer specificity was monitored with a melting curve analysis using the QuantStudio software V1.3 (Thermo Fisher Scientific). Relative expression of each hexamerin gene was determined using the ΔΔCt method [48] by normalizing the level of each hexamerin transcript to the internal control gene, rp18 which is stable among different S. invicta castes [49]. For each gene expression assay, six independent biological replicates were analyzed; one replicate consisted of one pool of ants.
Juvenile hormone analog study
Alate virgin queens and medium-size workers (nurses and foragers) were collected and treated topically with 1 μl of the JH analog, S-hydroprene (analytical standard catalogue number 46426, Sigma-Aldrich, St. Louis, MO) (25 ng/μl dissolved in acetone) with a pipette on the dorsal surface of the abdomen, or with 1 μl of acetone on the abdomen once during the assay. The chosen S-hydroprene dose was used in previous experiments on ants [10,41]. Fresh solutions of S-hydroprene were prepared for each biological replicate. Also included for each biological replicate was a non-treatment control group, which consisted of ants that were handled similarly to the treatment group but to which no solution was topically applied. Following treatment, ants from each treatment group were caged together in separate containers and placed within their original colony (S1 Fig). Cages (15 ml tube) were enclosed with very fine nylon mesh, which separated each group from the rest of the colony. Six replicates were conducted using six independent colonies. As expected, only the S-hydroprene treatment induced dealation of the alate virgin queens: 100% of the treated queens dealated while none of the non-treatment or acetone-treated virgin queens dealated. Therefore while the fine mesh separated individuals from the rest of the colony, it did not isolate them nor prevented contact with the other insects in the colony.
Ten medium-size foragers, medium-size nurses, or alate virgin queens from each treatment group were collected twelve hours after treatment, pooled by treatment, flash-frozen, and stored at -80°C until gene expression analyses. Total RNA extractions from each pool and RT-qPCR analyses were conducted as described before.
Statistical analysis
Data are reported as the mean ± the standard error of the mean (SEM). Data were log transformed and the normality of the log CT was verified after transformation. Statistical analyses were conducted using the one-way ANOVA test and Tukey-Kramer Post Hoc with JMP Version 13 (SAS Institute Inc., Cary, NC, 1989–2017) and the estimated p-values were considered significant when below the 0.05 threshold.
Results
Phylogenetic analysis
A Bayesian analysis was conducted to evaluate the phylogenetic association of S. invicta hexamerins with other hymenopteran hexamerins (Fig 1). Strong support for nodes was found on the phylogeny. Most nodes had 100% posterior probability value; two nodes did not but achieved 92% and 65% support. The obtained phylogenetic tree has two major hexamerin clades as in [50] which analyzed N. vitripennis hexamerins.
Node value posterior probability was rounded to two significant figures. Scale bar indicates branch length. The two hexamerins of the termite R. flavipes were used as the outgroup. The four clade names represent the four A. mellifera hexamerin proteins. The hexamerins of S. invicta (boxed in red) clustered as follows: Hexamerin 1 (XP_025995150.1) with Hex 70b, Hexamerin 2 (XP_011171862.1) with Hex 110, Arylphorin subunit alpha-like (XP_011155485.1) with Hex 70c, and Arylphorin subunit beta (XP_011155484.1) with Hex 70a. Alignment of the S. invicta hexamerin proteins can be found in supplementary information (S2 Fig).
The smaller hexamerin clade included the largest S. invicta hexamerin, Hexamerin 2, (S. invicta_hex2 in Fig 1) which clustered more closely with hexamerins from four ant species, H. saltator XP_011138808.1, A. colombica XP_018046967.1, A. echinatior XP_011054554.1, and C. floridanus XP_025264718.1 as well as with A. mellifera NP_001094493.1 (Hex 110), N. vitripennis NP_001164356.1 and N. vitripennis XP_001599110.3. Similar to the proteins from these other species, S. invicta Hexamerin 2 is characterized by a high glutamic acid and glutamine (Glx) content (25.4%) (Table 1). S. invicta Hexamerin 2 is located in the contig NW_020522001.1 in the S. invicta genome.
The other three S. invicta hexamerins, which are adjacent in the S. invicta genome on the same contig (NW_020521759.1), show a higher degree of similarity among themselves (Fig 1 and Table 2). S. invicta Hexamerin 1 clustered with A. mellifera NP_001011600.1 (Hex 70b). Interestingly, N. vitripennis XP_001605292.3 and XP_001600430.1, which had previously clustered with A. mellifera Hex 70b [50] were not in this cluster. S. invicta Arylphorin subunit alpha-like clustered with A. mellifera NP_001092187.1 (Hex 70c), and two N. vitripennis proteins, NP_001153417.1 and XP_016839327.1. Finally, S. invicta Arylphorin subunit beta clustered with A. mellifera NP_001104234.1 (Hex 70a) and N. vitripennis NP_001164332.1. Each of these S. invicta proteins were more similar to the ant hexamerins than to the A. mellifera or N. vitripennis hexamerins. Both S. invicta arylphorin proteins have high aromatic amino acid content (Table 1). Hexamerin 1 failed to meet the 15% aromatic amino acid criterion to be considered an arylphorin (Table 1). None of the S. invicta hexamerins are leucine-rich (>10% leucine) or methionine-rich (> 4% methionine) proteins [51].
Identities are shown above, similarities below the diagonal line represented by shaded cells.
Expression of hexamerins in workers and queens
The patterns of expression of hexamerin 1, hexamerin 2, arylphorin subunit alpha-like and arylphorin subunit beta transcripts were obtained for alate virgin queens, dealate mated queens, medium-size foragers, and medium-size nurses. Significant differences were found in the expression of all genes. Foragers had the lowest expression of all tested genes (Fig 2). For hexamerin 1 (Fig 2A), significant differences were found among the four groups (F = 2270.19, df = 3, p < 0.0001). Alate virgin queens had the highest level of expression, significantly higher than the other three groups, while dealate mated queens had significantly higher levels of expression compared to nurses and foragers, and nurses had significantly higher levels than foragers. Similar expression profiles were obtained for hexamerin 2 (Fig 2B) (F = 424.06, df = 3, p < 0.0001) and arylphorin subunit beta (Fig 2D) (F = 713.60, df = 3, p < 0.0001). Nurses had the highest levels of expression. Alate virgin and dealate mated queens had similar levels of expression, which was significantly higher than foragers. For arylphorin subunit alpha-like (Fig 2C), significant differences were found among groups (F = 671.87, df = 3, p < 0.0001). No significant difference in expression was found between nurses and alate virgin queens. Dealate mated queens had significantly lower expression than the nurses and alate virgin queens, but significantly higher than foragers.
Relative expression levels of (A) hexamerin 1, (B) hexamerin 2, (C) arylphorin subunit alpha-like, and (D) arylphorin subunit beta transcripts. Gene expression was quantified using RT-qPCR and analyzed using the ΔΔCt method. For each gene, mRNA expression level was normalized relative to rp18. Bars represents mean ± SEM fold change relative to foragers (n = 6). Different letters indicate significant differences in gene expression as determined by one-way ANOVA with Tukey’s post hoc analyses (p < 0.05). The fold change in the Y-axis is in log-scale.
Expression of hexamerins in medium-size foragers following topical application of S-hydroprene
The expression of the four hexamerin genes was evaluated from pools of ten individual medium-size foragers 12 hours after S-hydroprene application (Fig 3). No significant differences in expression were found for hexamerin 1 (Fig 3A) and arylphorin subunit alpha-like (Fig 3C) among the treatment groups (p > 0.05). However, significant differences in expression were identified for hexamerin 2 (F = 17.48, df = 2, p = 0.003, Fig 3B) and arylphorin subunit beta (F = 77.96, df = 2, p < 0.0001, Fig 3D). In both cases, expression was significantly lower in the S-hydroprene treatment when compared to both control treatments (non-treatment and acetone).
Relative expression of (A) hexamerin 1, (B) hexamerin 2, (C) arylphorin subunit alpha-like, and (D) arylphorin subunit beta transcripts. Gene expression was quantified using RT-qPCR and analyzed using the ΔΔCt method. For each gene, mRNA expression level was normalized relative to rp18. Bars represents mean ± SEM fold change relative to the untreated control (n = 6). Statistical relationships between groups were assessed using one-way ANOVA. Significant differences in gene expression (p < 0.05) were determined for hexamerin 2 and arylphorin subunit beta. For those genes, different letters indicate significant differences in gene expression as determined with Tukey post hoc analyses.
Expression of hexamerins in medium-sized nurses following topical application of S-hydroprene
The pattern of expression of the four hexamerin genes was evaluated from pools of ten individual medium-size nurses 12 hours after S-hydroprene application (Fig 4). In this case, the expression of all genes was significantly reduced in the S-hydroprene treatment group compared to the control treatments: hexamerin 1 (F = 4.79, df = 2, p = 0.025, Fig 4A); hexamerin 2 (F = 17.48, df = 2, p = 0.0001, Fig 4B); arylphorin subunit alpha-like (F = 36.88, df = 2, p < 0.0001, Fig 4C), and arylphorin subunit (F = 77.96, df = 2, p < 0.0001, Fig 4D).
Relative expression of (A) hexamerin 1, (B) hexamerin 2, (C) arylphorin subunit alpha-like, and (D) arylphorin subunit beta transcripts. Gene expression was quantified using RT-qPCR and analyzed using the ΔΔCt method. For each gene, mRNA expression level was normalized relative to rp18. Bars represents mean ± SEM fold change relative to untreated control (n = 6). Different letters indicate significant differences in gene expression as determined by one-way ANOVA with Tukey’s post hoc analyses (p < 0.05).
Expression of hexamerins in alate virgin queens following topical application of S-hydroprene
The pattern of expression of the four hexamerin genes was evaluated from pools of ten virgin queens 12 hours after S-hydroprene application. No differences in the expression of hexamerin 2 (Fig 5B) or arylphorin subunit beta (Fig 5D) were found among the treatments (p > 0.05), while differences were found for hexamerin 1 (F = 40.94, df = 2, p = < 0.0001, Fig 5A), and for arylphorin subunit alpha-like (F = 33.27, df = 2, p = <0.0001, Fig 5C). In both cases, the expression of was significantly lower in the S- hydroprene treatment group than in both control groups.
Relative expression of (A) hexamerin 1, (B) hexamerin 2, (C) arylphorin subunit alpha-like, and (D) arylphorin subunit beta transcripts. Gene expression was quantified using RT-qPCR and analyzed using the ΔΔCt method. For each gene, mRNA expression level was normalized relative to rp18. Bars represents mean ± SEM fold change relative to untreated control (n = 6). Significant differences in gene expression (p < 0.05) were determined for hexamerin 1 and arylphorin subunit alpha-like. For those genes, different letters indicate significant differences in gene expression as determined with Tukey post hoc analyses.
Discussion
In holometabolous insects, hexamerin proteins accumulate during larval development [51] and serve as an amino acid source during the pupal and pharate adult stages. Insects encode several hexamerins, and the copy number of hexamerin genes varies within social insects.. Four hexamerin genes are predicted in the S. invicta genome (LOC105192919, LOC105192898, LOC105204474, and LOC105192897). Based on the phylogenetic analysis, they correspond to each of the four hexamerin types found in A. mellifera (hex 70a, hex 70b, hex 70c, hex 110). This is consistent with previous analyses of hymenopteran genomes which typically harbor at least one copy of each of the four hexamerins commonly identified in A. mellifera [52]. Interestingly, our search of hexamerins among the chosen ant species failed to identify the homolog of hex 70c in the leaf-cutting ants A. colombica and in A. echinatior which could be linked with the mutualism established between these species and fungi [52]. In addition to hex 70c in the aforementioned ants, a homolog of hex 110 was not found in the current annotation of L. niger genome.
In S. invicta the four hexamerin genes were expressed in queens and in workers. The gene expression of all hexamerins was lower in foragers than in nurses or in queens. Hexamerin 2 and arylphorin subunit beta were expressed at higher levels in nurses than in queens, while arylphorin subunit alpha-like was expressed at higher levels in nurses than in mated queens, but not virgin queens. In ants, expression of hexamerin genes in adults, and differences of expression between the worker and queen castes were recorded in Formica exsecta [53]. In this species, all hexamerin transcripts are expressed at higher level in emerging queens than in emerging workers, while the opposite pattern is found in older insects because old workers have higher hexamerin transcript expression than old queens (Supporting information in [53]). Moreover, the accumulation of hexamerin 1 and 2 in virgin queens of Camponotus festinatus has been associated with claustral colony founding [39]. This is in contrast to A. mellifera in which the expression of hex 70a and hex 110 genes in queens is relatively low and occurs in the ovaries of egg-laying queens [37,54,55]. Further, these hexamerin genes are the only hexamerins with detectable expression in the A. mellifera adult worker fat body [37].
It is noteworthy that expression of all four S. invicta hexamerins was down-regulated in foragers when compared to nurses. This finding is in accordance with transcriptomic results comparing gene expression between S. invicta nurses and foragers (Supplementary data in [56]). Similarly, in A. mellifera, the expression of hex 70a and of hex 110 decreased as workers aged, and this decrease corresponded with their transition to foraging [37]. This change in gene expression coincides with the increase in the JH titer as the A. mellifera workers age. Therefore, the expression of hexamerins in social insects could be associated with the specific task being performed by the worker, with the age of the worker, or result from the interaction of both task and age and other factors. Comparison of the hex 110 and hex 70a expression between queenless A. mellifera workers showed activation of the expression of both genes in workers with active ovaries compared to those carrying inactive ovaries [57,58]. S. invicta nurses do not have the potential for reproduction; therefore, the expression of hexamerins in workers of this ant species is likely linked to other functions. As such, nurses could be using hexamerins as reserves to nourish brood and/or in nutritional signaling [29,41]. For instance, Camponotus festinatus workers accumulate Hexamerin 1 and Hexamerin 2 proteins when reared in the absence of brood, but not in the presence of larvae [39].
Previously, we had shown that hexamerin 1 expression in S. invicta virgin queens was down-regulated following topical application of a JH analog [41]. Here, we evaluated the expression of the four S. invicta hexamerin genes following topical application of the JH analog to virgin queens, nurses, and foragers. The four genes were down-regulated in S. invicta nurses after application of the JH analog. However, only hexamerin 2 and arylphorin subunit beta were down-regulated in foragers, while hexamerin 1 and arylphorin subunit alpha-like were down-regulated in virgin queens following the topical application of the JH analog. Therefore, it is possible that JH regulates hexamerins differentially in the reproductive caste than in worker caste. The reduced expression of all hexamerins in foragers compared to nurses, and the reduction of hexamerin expression following the topical application of the JH analog to nurses are consistent with the existence of a low JH titer in nurses and a high titer in foragers as shown in other ant species.
Based on the results presented in this study, we propose the existence of different regulatory modules controlling the expression of hexamerins in S. invicta in a caste-specific manner (Fig 6). In workers, JH regulates the expression of the four hexamerin genes: the four genes were down-regulated in nurses following the JH analog application and in foragers hexamerin 2 and arylphorin subunit beta were also down-regulated by JH. It is possible that RT-qPCR could not detect a reduction in the expression of hexamerin 1 and of arylphorin subunit alpha-like following the application of the JH analog in foragers due to low expression of these two genes in these workers. Importantly, the fact that application of the JH analog further reduced the expression of hexamerin 2 and arylphorin subunit beta in medium size foragers may reflect the molecular mechanism for behavioral plasticity in medium size worker foragers; that is, increase their foraging task in response to higher levels of JH, or perhaps these ants have the ability to revert to in-nest tasks in special circumstances when JH levels may decrease.
In workers, hexamerin expression is regulated by JH. The expression of the four hexamerins decreases as workers transition from nursing to foraging tasks, which supports the existence of changes in JH as the workers age (represented in dashed). In queens, only hexamerin 1 and arylphorin subunit alpha-like are regulated by JH. Both genes are down-regulated in mated queens compared to virgin queens.
Regulation of hexamerins by JH is different in queens. In this caste only hexamerin 1 and arylphorin subunit alpha-like appear to be regulated by JH. This conclusion is based on the down-regulation of these genes but not of hexamerin 2 and arylphorin subunit beta in virgin queens following the JH analog application (Fig 5). The expression analyses performed using mated and virgin queens support this finding (Fig 2). Alate virgin queens are repressed by the queen primer pheromone, and as a result they have low levels of JH, which prevent their ovaries from developing [59]. When virgin queens are released from the influence of the queen primer pheromone, their JH titers increase and as a consequence they shed their wings and their ovaries develop. The same results of wing shedding and ovary development are obtained by topical application of JH or its analogs [41,59,60,61,62]. The changes in hexamerin 1 and arylphorin subunit alpha-like gene expression in alate virgin queens could be linked to the mobilization of reserves for ovary development. Additionally, these changes could also be linked to the potential involvement of hexamerins in signaling nutritional status; the down-regulation of these hexamerins could signal the end of the period in which virgin queens actively accumulate reserves.
Some hexamerins bind JH [31,63], thus it is hypothesized that these proteins might bind JH in social insects to modulate JH titers [29]. Among the S. invicta hexamerins, arylphorin subunit alpha-like contains a region that is the most similar to the sequence of the locust hexamerin, which if deleted, eliminates the JH binding activity [41,63]. Therefore, if the arylphorin subunit alpha-like protein has a similar expression pattern as the transcript, this protein could be involved in modulating JH titers in different S. invicta castes: in queens, it might be involved in maintaining a low JH titer in virgins to prevent precocious reproductive development [64]; while in workers, it might be involved in maintaining a low JH titer in nurses preventing transition to foraging tasks. Indeed, JH titer is low in the A. mellifera nurse and it increases as the insect ages [18,20,23,24]. While JH titers in S. invicta workers have not been measured, the results presented here support a similar role of JH in S. invicta age polyethism such as that found in other social insect species: all hexamerins were expressed at lower levels in foragers compared to nurses (Fig 2). Further, because all hexamerins were down-regulated following the application of a JH analog to nurses (Fig 4), our results also support the existence of changes in JH titers as the S. invicta worker ages.
One pitfall of the present study is that the ants used were of unknown age. The insect age could be a factor affecting the level of hexamerin expression in the alate virgin queens and the workers. The existence of age polyethism in S. invicta workers was established by Mirenda and Vinson [65] who determined that young workers stay in the nest where they perform nest-related tasks; then,they take more peripheral locations as they age, and they become forages as they near their death. While we can speculate that in the present study the foragers were older than the nurses, future studies should address the effect of age in the expression of these genes.
In conclusion, our study found a caste- and task-biased expression of hexamerins in S. invicta. Further, the regulation of the four genes by the JH analog S-hydroprene was also established, however this regulation was caste-specific for two of these genes.. While expression of these genes in A. mellifera workers might be associated with their potential for reproduction, S. invicta workers cannot reproduce. Thus, the expression of hexamerins might indicate the potential role of these genes in other aspects of S. invicta biology. In queens, hexamerin 1 and arylphorin subunit alpha-like may be associated with signaling nutritional status, dominance status and/or reproductive activity. It is tempting to speculate that the lack or low level of regulation of hexamerin 2 and arylphorin subunit beta in queens in response to the JH analog (Fig 5) could be pre-determined during the caste differentiation of reproductives by a mechanism involving DNA changes (methylation/demethylation), for example. Alternatively, in queens, other signaling factors have higher hierarchy to indicate the nutritional status as JH is a gonadotropin. However, it is also possible that the observed lack of regulation results from the analysis of RNA from whole body samples, thus tissue-specific analysis are needed to verify this result. In workers, hexamerin expression might be associated with nutritional signaling, task-allocation and/or age polyethism. Indeed, hexamerins in the worker caste may reflect the nutritional status of workers because foragers have the lowest levels of expression, reflecting the starved phenotype [66], and nurses, which are preferentially fed and have a higher nutritional status, have higher relative levels of all hexamerins (Fig 2). In addition, this is demonstrated in that the level of hexamerin expression in nurses can be downregulated by application of a JH analog, which would mimic the transition to foraging, again, reflecting a starved phenotype. The medium foragers, however, with lower endogenous levels of hexamerins, do not respond equally to the JH analog perhaps because the expression of two hexamerins has already decreased to a minimum level, and only hexamerin 2 and arylphorin subunit beta respond to the treatment. Co-option of hexamerin genes may have given S. invicta colony the ability to respond to cues involved in social organization and queen dominance, and the expression of these storage proteins in adults may be based on colony demands. The gene expression analyses presented here illustrates the potential for hexamerins to play important roles in fire ant social physiology, warranting protein level analysis and reverse genetic manipulation to further elucidate the evolutionarily coopted roles of these genes/proteins in relation to the reproductive ground plan hypothesis.
Supporting information
S1 Fig.
Constructed cages for treatment groups of queens: (A) Materials used for the cage are a 15 mL tube, fine netting cut into a square, parafilm to hold down the netting. (B) Constructed treatment cage. (C) After treatment alate queens were put into tubes and lid fastened. (D) Cages were put back into the colony. (E) 100% of alate queens from the S-hydroprene treatment dealated.
https://doi.org/10.1371/journal.pone.0216800.s001
(TIFF)
S2 Fig. Alignment of the four S. invicta proteins using MAFT.
() indicates non conservative mutations, (*) indicates conserved regions, (:) indicates conservative replacement mutations and (.) indicates semi conservative mutations.
https://doi.org/10.1371/journal.pone.0216800.s002
(DOCX)
Acknowledgments
Dr. Robert Puckett, Assistant Professor, Dept. of Entomology, Texas AgriLife at TAMU provided valuable assistance in locating and collecting colonies. Dr. Carl Hjelmen for his assistance with Mr. Bayes and valuable discussion. Grayson Tung and Dr. Juliana Rangel for revisions. Franchesca Rodriguez and Andrew Renfro both undergraduate students in the Department of Entomology helped maintain fire ant colonies in the laboratory. C. Hawkings is a Ph.D. candidate in the Entomology graduate program at TAMU. The A.W.E.S.O.M.E. faculty group of the College of Agriculture and Life Sciences, TAMU, is acknowledged for editing the manuscript.
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