In both Drosophila and the mouse, the zinc finger transcription factor Snail is required for mesoderm formation; its vertebrate paralog Slug (Snai2) appears to be required for neural crest formation in the chick and the clawed frog Xenopus laevis. Both Slug and Snail act to induce epithelial to mesenchymal transition (EMT) and to suppress apoptosis.
Methodology & Principle Findings
Morpholino-based loss of function studies indicate that Slug is required for the normal expression of both mesodermal and neural crest markers in X. laevis. Both phenotypes are rescued by injection of RNA encoding the anti-apoptotic protein Bcl-xL; Bcl-xL's effects are dependent upon IκB kinase-mediated activation of the bipartite transcription factor NF-κB. NF-κB, in turn, directly up-regulates levels of Slug and Snail RNAs. Slug indirectly up-regulates levels of RNAs encoding the NF-κB subunit proteins RelA, Rel2, and Rel3, and directly down-regulates levels of the pro-apopotic Caspase-9 RNA.
These studies reveal a Slug/Snail–NF-κB regulatory circuit, analogous to that present in the early Drosophila embryo, active during mesodermal formation in Xenopus. This is a regulatory interaction of significance both in development and in the course of inflammatory and metastatic disease.
Citation: Zhang C, Carl TF, Trudeau ED, Simmet T, Klymkowsky MW (2006) An NF-κB and Slug Regulatory Loop Active in Early Vertebrate Mesoderm. PLoS ONE 1(1): e106. doi:10.1371/journal.pone.0000106
Academic Editor: Carl-Philipp Heisenberg, Max Planck Institute of Molecular Cell Biology and Genetics, Germany
Received: October 26, 2006; Accepted: November 23, 2006; Published: December 27, 2006
Copyright: © 2006 Zhang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was initiated with support from the March of Dimes Birth Defects Foundation, Inc., and completed with support from the National Institutes of Health (GM54001) and the American Heart Association. TS was supported by the German Cancer Foundation (Deutsche Krebshilfe).
Competing interests: The authors have declared that no competing interests exist.
The process of transforming relatively immotile epithelial cells into actively migrating mesenchymal cells, known as epithelial to mesenchymal transition (EMT), is central to a wide range of biological processes from mesoderm, mesenchyme, and neural crest formation to pathogenic fibrosis and metastasis –. Important players in the regulation of EMT are the zinc finger transcription factors Snail (Snai1) and its vertebrate paralog Slug (Snai2). In addition to Snail and Slug, a number of other members of the Snail family have been identified. In Drosophila melanogaster there are the Snail-like genes Escargot and Worniu –, and the more divergent Scratch genes . The duplication event that gave rise to Snail-like and Scratch-like genes appears to have occurred before the divergence of proteostomes and deuterostomes , .
The involvement of Snail-like proteins in EMT was first suggested by genetic studies in Drosophila. Mutations in Snail lead to the disruption of mesoderm and embryonic lethality –. As in Drosophila, mice homozygous for a null mutation in the orthologous Snail gene fail to form normal mesoderm and exhibit early embryonic lethality . No mesodermal phenotype was observed in mice homozygous for a null mutation in Slug , . The absence of Slug does lead to defects in melanocyte, hematopoietic stem cell and germ cell development, and epidermal healing –. Slug is expressed in the mesoderm in the chick and exposure of the early embryo to anti-sense oligonucleotides leads to defects in mesoderm emergence . In X. laevis Slug mRNA is expressed zygotically in the dorsal mesendoderm; interference with its function, through the injection of RNAs encoding dominant negative proteins, leads to defects in the expression of organizer (Chordin, Cerberus) and ventrally (Xwnt8, Xvent1) expressed genes . An important concern about such studies involves the specificity of “anti-morphic” reagents, given the known regulatory cross-talk between E-box binding Snail, Slug and basic helix-loop-helix (bHLH) transcription factors (see below).
In both X. laevis and the chick, Slug appears to have an essential role in neural crest formation , –. In contrast, mutation of Slug has no apparent effect on neural crest formation in the mouse . This apparent discrepancy was initially ascribed to a swapping of Slug and Snail expression domains in the mouse , . More recent studies, using a combination of constitutive and conditional knock out mutations, indicate that neither Slug nor Snail are required for neural crest formation in the mouse, at least in the cranial region .
Snail-like proteins are generally thought to act as transcriptional repressors, although Sakai et al  report that Slug positively regulates is own expression. Snail, Slug, and Scratch all bind to E-box sequences (CANNTG) and can antagonize the activity of bHLH proteins , –. In their role as regulators of EMT, Slug and Snail have been found to suppress expression E-cadherin and tight junction components and the forced expression of Slug disrupts adherens junctions, tight junctions, and desmosomes –. Slug and Snail also act as inhibitors of apoptosis –. Slug has been found to negatively regulate the expression of the pro-apoptotic p53  and Puma  genes. Subsequent studies have found that Slug is required for the metastasis of human melanoma cells  and has been implicated in lung adenocarcinoma and breast carcinoma invasiveness –.
Sequence analysis indicate that Slugs are more conserved than vertebrate Snails . Lespinet et al  grouped chick (Gallus gallus) and X. laevis Snails with Slugs rather than with other vertebrate Snails. Slug and Snail have been found to be functionally similar, but not identical. For example, injection of RNA encoding Snail rescues the effects of anti-sense Slug RNA injection in X. laevis  and “Slug and Snail can be can be functionally equivalent when tested in overexpression studies” . Over-expression of Snail leads to expansion of the neural crest domain in the chick, much as observed following over-expression of Slug , . On the other hand, the need for Snail expression in the early Drosophila embryo cannot be replaced by either Escargot or Worniu . Snail and Slug differ in their ability to induce neural crest markers in X. laevis ectodermal explants , even though Slug alone has been found to rescue the effects on neural crest following the blocking of both Slug and Snail activity [see 24]. Slug appears to bind less strongly to regulatory regions in the E-cadherin protein than does Snail , while Slug, but not Snail, has been found to mediate genotoxin resistance in human mesothelioma cells . A microarray-based analysis of MDCK epithelial cells found both common and distinct sets of genes regulated by Slug and Snail . Given that Snail – and Slug  can be post-translationally regulated in terms of both stability and intracellular localization, it remains unclear whether the differences between the two proteins are intrinsic or are due to protein-specific post-translational effects.
Previous studies of Slug's role in X. laevis have used either anti-sense RNA  or dominant-negative proteins , , , ,  to disrupt Slug expression and/or activity. As part of a study to separate the role of Slug in EMT from its role as a regulator of apoptosis, we designed a modified anti-sense DNA oligonucleotide (a morpholino) that blocks Slug expression. In the course of analyzing the ability of the anti-apoptotic protein Bcl-xL to rescue the phenotypic effects of this morpholino, we uncovered an essential role for NF-κB as a regulator of Slug expression in the early embryo, a regulatory interaction analogous to that observed in the early Drosophila embryo, and not apparently described previously in a vertebrate.
Previous studies on the role of Slug in Xenopus have relied on injection of either anti-sense RNA directed against 3′ untranslated region of the SlugA mRNA  or RNAs encoding various dominant-negative proteins , , , , . To complement these studies, we developed a morpholino (Slug MO) directed against the Slug mRNAs. There are two Slug pseudoalleles in X. laevis, SlugA and SlugB . The Slug MO is a perfect match to the SlugA mRNA, has three mismatches to the SlugB mRNA and 12 (out of 25) mismatches with the analogous region of the Snail mRNA (FIG. 1A). The Slug MO blocked the in vitro translation of SlugA RNA that contained its target sequence but had no effect on the translation of mycGFP-Slug RNA, which lacks SlugA's 5′ untranslated region (data not shown).
Figure 1. Slug morpholino effects:
Panel A is a comparison of the Slug morpholino sequence (“MO”) with X. laevis SlugA, SlugB, and Snail RNA sequences; start codons are underlined. B–G: Injection of the Slug MO (10 ng/embryo) blocks the expression of Xbra (B-uninjected, C-Slug MO injected), Xmenf (D-uninjected, E-injected), and Antipodean (Apod)(F-uninjected, G-injected). Arrows (C,E,G) point to region of suppression; vegetal pole (“VP”) is indicated. In G, the red staining is due to a β-galactosidase lineage marker. H: Injection of the Slug MO into one cell of a two cell embryo blocks the expression of Sox9 on the injected side (arrow); “*” marks otic placode domain of Sox9 expression. I: Sox9 expression in Slug MO injected embryos is rescued by co-injection of mycGFP-Slug RNA (650 pg/embryo). In analogous studies, the effects of the Slug MO (10 ng/embryo) on Xbra (J), Apod (K) and Sox9 (L) expression were rescued by injection of Snail RNA (500 pg/embryo). In H, I and L, the line marks midline of the embryo, with anterior (“An”) and posterior (“Ps”) indicated.doi:10.1371/journal.pone.0000106.g001
When injected into one cell of two-cell embryos, the Slug MO (10 ng/embryo) inhibited expression of the mesodermal markers Xbra (FIG. 1B,C), Xmenf (FIG. 1D,E), and Antipodean (Apod)(FIG. 1F,G) in late blastula/early gastrula stage embryos. In neurula stage embryos, the Slug MO inhibited expression of Sox9 (FIG. 1H), a marker of cranial neural crest and otic placodes , . In later stage embryos, the Slug MO led to the loss of craniofacial cartilages and the otic vesicle (data not shown), very much as observed in embryos injected with Slug anti-sense RNA . Injection of a control MO had no apparent effect on any of the markers examined (Table 1). As a control for the specificity of the Slug MO, embryos injected with Slug MO were injected with RNA encoding mycGFP-tagged Slug; both normal Sox9 expression (FIG. 1I) and craniofacial morphology (data not shown) were rescued; injection of RNA encoding myc-GFP did not rescue either phenotype (Table 1 and data not shown). Previously, we found that injection of Snail RNA rescued the phenotypic effects of anti-sense Slug RNA injection . This is also the case with the Slug morpholino; injection of 500 pg/embryo Snail RNA rescued expression of both mesodermal and neural crest markers in Slug MO injected embryos (FIG. 1J–L; Table 1).
Table 1. Slug Morpholino and Rescue experimentsdoi:10.1371/journal.pone.0000106.t001
Maternal Slug RNA can be detected by RT-PCR [22 and data not shown] and is expressed zygotically in mesoderm . The loss of Slug function during late bastula/early gastrula stages would be expected to influence both neural crest and placodal development, which depend upon signals from the mesoderm –. We generated plasmids that encode chimeric proteins consisting of glucocorticoid-binding regulatory domain ,  and either Slug alone (GR-Slug) or Slug linked to a C-terminal GFP moiety (GR-Slug-GFP). In the absence of dexamethasone, both GR-Slug proteins are inactive and had no apparent effect on the Slug MO's ability to block Sox9 expression (Table 2). When Slug MO and GR-Slug RNA injected embryos were exposed to dexamethasone beginning at stage 11, Sox9 expression was efficiently recovered (Table 2).
Table 2. Timing of Slug Rescuedoi:10.1371/journal.pone.0000106.t002
To examine the timing of Slug's role in neural crest formation, we compared the effects of activating the GR-Slug-GFP protein in mid-blastula (stage 8), early gastrula (stage 11), and late gastrula/early neurula (stage 13) embryos (FIG. 2; Table 2). Activation of Slug at stage 8 lead to a complete rescue of both mesodermal (Xbra) and neural crest/placodal (Sox9) marker expression. Efficient rescue of neural crest/placodal marker expression was also observed when Slug was activated at stage 11, but rescue was much less efficient when Slug was activated at stage 13 (FIG. 2; Table 2).
Figure 2. Timing of Slug rescue of Slug MO phenotypes:
To analyze the timing of Slug activity in the early embryo, we injected one cell of two cell embryos with Slug MO (10 ng/embryo) together with RNA (650 pg/embryo) encoding the chimeric GR-Slug-GFP protein. A: In the absence of the activating drug dexamethasone, the Slug MO phenotype, i.e. suppression of Xbra expression in stage 11 embryos (A)(arrow) and suppression of Sox9 expression in stage 16 embryos (D), was unaltered. When embryos were treated with dexamethasone (20 µM) beginning at stage 8, there as an essentially complete rescue of Xbra (B,C) and Sox9 expression (E,H). Treatment of embryos with dexamethasone at stage 11 (early gastrulation) was also effective at rescuing Sox9 expression (F,H), while addition of dexamethasone at stage 13 (late gastrulation/early neurulation) produced at most a partial and inefficient rescue of Sox9 expression (G,H).doi:10.1371/journal.pone.0000106.g002
Bcl-2/Bcl-xL suppression of the Slug MO phenotype
Inhibition of Slug activity by injection of Slug MO (FIG. 3A), antisense RNA (data not shown) or RNA encoding a dominant negative version of Slug (ZnfSlug) leads to increased numbers of apoptotic cells as visualized by TUNEL staining, while injection of Slug RNA suppresses apoptosis . In our studies, carried out at stage 16/17, the increase in TUNEL positive cells was most prominent outside of the neural crest stage Slug expression domain, and so presumably represent effects on earlier developmental events.
Figure 3. Rescue of Slug MO effects by Bcl-xL
A: Injection of the Slug MO leads to an increase in TUNEL staining. B: This increase is blocked by the injection of Bcl-xL RNA (600 pg/embryo and co-injected with LacZ RNA). Injected sides of embryos are marked by an “*” and red staining; line marks midline of the embryo, with anterior (“An”) and posterior (“Ps”) indicated. Injection of Bcl-xL RNA (600 pg/embryo) rescues Apod (C- Slug MO injected, D-Slug MO+Bcl-xl RNA injected), Xbra (Ε- Slug MO injected, F-Slug MO+Bcl-xl RNA injected), and Sox9 expression (G- Slug MO injected, H-Slug MO+Bcl-xL RNA injected). I: Injection of Bcl-xL RNA into one cell of a two-cell embryo led to a dramatic increase in the intensity and extent of Slug expression at stage 16; the region of Slug expression on the uninjected (control) side of the embryo is indicated by the dashed circle. J: Injection of Bcl-xL RNA produced an increase in Slug RNA levels in animal caps prepared at stage 8/9 and analyzed by QRT-PCR when uninjected embryos reached stage 11. Ornithine decarboxylase (ODC) was used as normalization control. RNA levels in the control case were set to 100%.doi:10.1371/journal.pone.0000106.g003
To distinguish Slug's pro-EMT and anti-apoptotic activities, we injected embryos with RNAs encoding the anti-apoptotic proteins Bcl-2 (human) and Bcl-xL (X. laevis); both had similar effects and observations using Bcl-xL are show here. As expected, injection of Bcl-xL RNA blocked the Slug MO induced increase in TUNEL staining (FIG. 3B). Bcl-xL also rescued the expression of the early mesodermal markers Apod (FIG. 3C,D) and Xbra (FIG. 3E,F), the neural crest/otic placode marker Sox9 (FIG. 3G,H; Table 1), and craniofacial morphology (data not shown). In stage 16/17 embryos, injection of Bcl-xL RNA led to a dramatic increase in the level and spatial extent of Slug expression (FIG. 3I). In ectodermal explants (animal caps), prepared at stage 8/9 from embryos injected with Bcl-xL RNA, and analyzed at stage 11 (~3 hours later), there was a small (~2×) but reproducible increase in the level of Slug mRNA, as determined by quantitative RT-PCR (FIG. 3J).
Bcl-xL and Slug regulate NF-κB activity
Bcl-2 and Bcl-xL are structurally similar cytoplasmic proteins. Bcl-2 can influence gene expression through effects on IκB kinase activity (IκK)–. To examine Bcl-xL's effects on NF-κB activity in Xenopus, we used the NF-κB responsive p3XκB-Luc reporter plasmid together a mutated form of human IκBα (IκBsa) and acetyl-11-keto-β-boswellic acid (AKBA). In IκBsa serines 32 and 36, normally phosphorylated by IκK, are mutated to alanines. The stable IκBsa polypeptide acts as a dominant repressor of NF-κB activity . AKBA inhibits IκK activation –. Treating animal caps with 50 µM AKBA stabilized an epitope-tagged form of Xenopus IκBα (FIG. 4A). Fertilized eggs were injected with p3XκB-Luc plasmid DNA, pTK-Renilla luciferase plasmid DNA (as a normalization control), and Bcl-xL RNA alone or together with IκBsa RNA; animal caps were prepared and analyzed for luciferase activity at stage 11. Alternatively, fertilized eggs were injected with p3XκB-Luc and pTK-Renilla DNAs and Bcl-xL RNA, animal caps were prepared and then incubated in control media or in media containing AKBA. Bcl-xL increased NF-κB reporter activity and this increase was blocked by both IκBsa and AKBA (FIG. 4B). On its own, AKBA had little effect on reporter activity.
Figure 4. Characterization of Bcl-xL effects on NF-κB activity.
A: Fertilized eggs were injected with RNA (650 pg/embryo) encoding Xenopus IκBα-V5. Beginning at stage 8, experimental embryos were treated with 50 µM AKBA and analyzed at stage 11 by SDS-PAGE/immunoblot using an anti-V5 antibody and the antiSOX3c antibody to visualize endogenous Sox3 protein as a loading control. AKBA treatment stabilized the IκBα-V5 polypeptide. B: Fertilized eggs were injected with p3XκB-firefly luciferase (“3κB-Luc”) and pTK-Renilla luciferase (“RL-TK”) DNAs (10 pg/embryo each) either alone (“Con”) or together with Bcl-xL (500 pg/embryo) RNA, or Bcl-xL and IκBsa (600 pg/embryo) RNAs. Alternatively, animal caps prepared from Bcl-xL RNA injected embryos were cultured in either control buffer (0.1% DMSO), 20 µM or 50 µM AKBA. At stage 11, caps were analyzed for luciferase activity. Bcl-xL induced an increase in 3XκB-Luc activity that was blocked by either IκBsa or AKBA. Error bars in B reflect standard deviation from the mean of multiple experiments.doi:10.1371/journal.pone.0000106.g004
Bcl-xL and Slug regulation of Rel expression
Five NF-κB subunit genes have been characterized in X. laevis: RelA/Rel1 , , Rel2 , Rel3 , RelB  and Xp100 . All five RNAs are supplied maternally and their levels drop at the onset of zygotic transcription (stage 8/9)(FIG. 5A). As development proceeds Rel2, Rel3 and Xp100 RNAs are again detectable by RT-PCR (28 cycles), while RelB RNA does not reappear until after stage 35 . Bcl-xL RNA levels appear constant throughout this period (FIG. 5A). In stage 16/17 embryos, RelA, Rel2, Rel3, and Xp100 RNAs can be readily detected by RT-PCR in the anterior-dorsal quadrant of the embryo; the same region where Slug and Sox9 are normally expressed (FIG. 5B). RelA appears concentrated in the anterior dorsal sector, while Rel2 and Rel3, and to a lesser extend Xp100 appear to be present at similar levels throughout the embryo.
Figure 5. Bcl-xL regulation of NF-κB RNAs:
A: RNA was extracted from eggs and embryos at various stages and analyzed by RT-PCR (28 cycles); levels of RelA, Rel2, Rel3, RelB and Xp100 RNAs drop between stage 7 and 9 and, except for RelB, increase following gastrulation (stage 12/13). Levels of Bcl-xL RNA appear relatively constant throughout this period of development. B: At stage 16, embryos were dissected into anterior dorsal (AD), posterior dorsal (PD), anterior ventral (AV), and posterior ventral (PV) quadrants and RNA was analyzed by RT-PCR; RelB was not expressed at this stage; expression of RelA, Slug and Sox9 are restricted to the anterior dorsal quadrant, while Bcl-xL, Rel2, Rel3, and Xp100 RNAs can be detected throughout the embryo. C, D: Animal caps were prepared from embryos injected with GR-Bcl-xL-GFP RNA (“GRBclxL”)(600 pg/embryo) and either left untreated (0.1%DMSO)(“Con”), treated with 20 µM dexamethasone (“+Dex”), treated first with 100 µg/ml emetine and then dexamethasone (“+Dex+Eme”), or treated with emetine alone (“+Eme”), and analyzed at stage 11 for Slug, RelA (C), Rel2 and Rel3 (D) RNA levels. Treatment with emetine blocked the increase in Slug and Rel2, but not RelA and Rel3 RNAs; emetine treatment alone produced control or slightly reduced levels of Slug and Rel RNAs. E: Activation of the GR-Bcl-xL-GFP protein in embryos injected with the Slug MO produces an increase in the level of Snail RNA, analyzed at stage 11. F: In animal caps derived from GR-BclxL-GFP RNA injected embryos, AKBA (50 µM) inhibited the dexamethasone-induced increases in Slug, Snail, and RelA RNA levels; while treatment with AKBA alone lead to a decrease in Slug, Snail and RelA RNA levels. G: In animal caps, injection of RelAΔSP RNA (600 pg/embryo) blocked Rel3 and Xp52 RNA induced activation of the 3XκB reporter. H: The ability of Bcl-xL RNA to increase levels of RelA and Slug RNAs in animal caps was blocked by the co-injection of RelAΔSP RNA. Error bars in C–H reflect standard deviation from the mean of multiple experiments.doi:10.1371/journal.pone.0000106.g005
To explore the mechanism of Bcl-xL regulation of Slug and NF-κB, we generated a plasmid encoding a glucocorticoid-binding domain-Bcl-xL-GFP (GR-BclxL-GFP) chimera. In the absence of dexamethasone, GR-BclxL-GFP does not alter Slug, RelA, Rel2 or Rel3 RNA levels, while addition of dexamethasone leads to their increase (FIG. 5C,D), similar to that seen using the non-hormone regulated form of Bcl-xL (FIG. 3J). No effect was observed on Xp100 RNA levels (data not shown). While these effects are small, i.e., 2–3 fold, they are highly reproducible. Adding the protein synthesis inhibitor emetine blocks the dexamethasone-dependent increase in Slug and Rel2 RNA levels, but not the increase in RelA or Rel3 RNA levels; emetine alone had no reproducible effect on RNA levels (FIG. 5C,D). These results suggest that Bcl-xL acts directly to regulate RelA and Rel3, and indirectly to regulate Slug and Rel2 RNA levels. By “direct” we mean a regulatory interaction that does not require on-going protein synthesis (see Discussion). Bcl-xL activation also leads to an increase in the level of Snail RNA (FIG. 5E); this increase does not appear to involve effects on Slug RNA, since it occurs in the presence of the Slug morpholino.
AKBA treatment blocked Bcl-xL's ability to increase levels of Slug, Snail and RelA RNAs (FIG. 5F), suggesting that Bcl-xL acts on these RNAs, as it does on the NF-κB responsive reporter, by increasing IκB kinase activity. RelAΔSP is a dominant negative form of RelA ; it dimerizes with other NF-κB subunit proteins and blocks their activity. When co-injected with RNAs encoding Rel3 or Xp52, the active form of the NF-κB subunit protein Xp100 , RelAΔSP inhibited their ability to activate of the 3XκB-Luc reporter (FIG. 5G) and inhibited Bcl-xL's ability to increase RelA and Slug RNA levels (FIG. 5H), indicating that active NF-κB is required for Bcl-xL to induce increases in Slug and RelA RNA levels.
Assuming that Bcl-xL acts to rescue the effects of the Slug MO through its ability to regulate NF-κB activity, injection of RelA RNA should be able to rescue the Slug MO phenotype. In embryos injected unilaterally with the Slug MO, injection of RelA RNA lead to re-appearance of both Xbra (FIG. 6A,B) and Apod (FIG. 6C,D) RNAs. In later stage, Slug MO-injected embryos, injection of RelA RNA lead to the reappearance of Sox9 expression in both the neural crest and otic placode regions, (FIG. 6E,F). A similar rescue of Sox9 expression in Slug MO injected embryos was observed upon injection of Rel3, or Xp52 RNAs (600 pg/embryo)(Table 1). We have not examined RelA's effects on Slug RNA in Slug MO injected embryos because morpholinos typically stabilize, rather than induce the degradation of, their target RNAs (unpubl. obs.); RelA does induce an increase in Slug RNA levels, as monitored by in situ hybridization, when injected on its own (data not shown – see below).
Figure 6. NF-κB regulation of mesodermal and neural markers:
The Slug MO induced loss of Xbra (A,B), Apod (C,D) and Sox9 (E,F) expression was rescued by injection of RelA RNA (600 pg/embryo)(A, C, E-Slug MO alone, B, D, F-Slug MO+RelA RNA). G: Treatment of early embryos with AKBA (50 µM from the 4-cell stage on) lead to a decrease in Xbra staining (control and AKBA-treated embryos marked). Injection of RNA encoding IκBsa (H,I) or RelAΔSP (J–L) had effects similar to that seen in Slug MO injected embryos; that is, both induced the reduction of Xbra (H,J), Apod (K) and Sox9 (I,L) RNA staining. AKBA treatment had no reproducible effect on Sox9 expression (data not shown). Arrows mark affected regions.doi:10.1371/journal.pone.0000106.g006
To complement these studies, we examined the effects of treating embryos with AKBA or injecting one cell of two cell embryos with either IκBsa or RelAΔSP RNAs. AKBA treatment, beginning at the 4-cell stage, led to a noticeable decrease in the intensity of Xbra RNA staining in early gastrula stage embryos (FIG. 6G), but had little reproducible effect on Sox9 RNA levels in neurula stage embryos (data not shown). Injection of IκBsa RNA lead to the suppression of Xbra (FIG. 6H; Table 1) and the reduction of Sox9 (FIG. 6I) expression. Injection of RelAΔSP RNA inhibited expression of Xbra (FIG. 6J), Apod (FIG. 6K), and Sox9 (FIG. 6L).
NF-κB regulation of Slug
To examine whether of NF-κB directly regulates Slug and Snail RNA levels, we first characterized the effects of RelA and RelAΔSP in animal caps; injection of RelA RNA lead to an increase, while RelAΔSP RNA lead to a decrease in Slug RNA levels (FIG. 7A). Using a dexamethasone-regulated form of RelA, GR-RelA, we found a similar effect – in the presence of dexamethasone GR-RelA produced an emetine-insensitive increase in Bcl-xL (FIG. 7B), Slug, and Snail RNAs (FIG. 7C). Given RelA's ability to induce Sox9 expression in Slug MO injected embryos (see above), we examined RelA's effect on Sox9 RNA levels; activation of RelA led to an increase Sox9 RNA levels even in the presence of emetine (FIG. 7C).
Figure 7. NF-κB's regulatory targets:
A: In animal caps, RelA lead to an increase in Slug RNA levels, while RelAΔSP produced a decrease. When activated by dexamethasone (+Dex), the hormone-regulated form of RelA, GR-RelA (600 pg RNA/embryo), induced a similar increase in the levels of Slug RNA, as well as Snail, Sox9 (B), and Bcl-xL (C) RNAs compared to animal caps from GR-RelA injected embryos not exposed to dexamethasone. Similar effects were seen in the presence of emetine (+Dex+Eme,), while emetine alone (+Eme) had little effect on any of measured RNA levels. Error bars in reflect standard deviation from the mean of multiple experiments.doi:10.1371/journal.pone.0000106.g007
Targets of Slug regulation
In Drosophila Dorsal, which encodes a RelA homolog, regulates Snail expression, and Snail in turn regulates Dorsal expression [see 90], . In animal caps, the Slug MO decreased and mycGFP-Slug increased RelA RNA levels (FIG. 8A), suggesting an analogous regulatory circuit. To characterize Slug's interactions with regulatory targets, we used the GR-Slug construct; its activity in the presence of dexamethasone is similar to that of mycGFP-tagged and untagged versions of Slug. In animal caps, both mycGFP-Slug and dexamethasone-activated GR-Slug lead to increased levels of the neural crest marker Zic5 , (data not shown) and Sox9 (FIG. 8B). In contrast Aybar et al.,  reported that Slug did not induce neural crest markers in animal caps analyzed at stage 20, ~22 hours after fertilization. To reconcile these observations, we analyzed animal caps derived from embryos injected with Slug RNA at stage 11 (our standard analysis time point) and stage 16; Sox9 RNA levels were increased at stage 11 but had returned to control levels by stage 16 (FIG. 8C), indicating that factors in addition to Slug are required to maintain Sox9 expression. In independent studies we have found that levels of Sox3 and SoxD RNAs, whose expression is associated with early germ layer and neural differentiation, change dramatically in the period between stage 11 and 14 (C. Zhang, T. Grammer & M.W. Klymkowsky, unpubl. obs). GR-Slug positively but indirectly increased levels of Bcl-xL (FIG. 8D), Sox9 (FIG. 8E), RelA, Rel2, and Rel3 RNAs (FIG. 8F), but had no effect on Xp100 RNA levels (data not shown).
Figure 8. Slug's regulatory targets:
A: In animal caps analyzed at stage 11, the Slug MO (10 ng/embryo) produced a decrease in RelA RNA levels that was rescued by co-injection of mycGFP-Slug RNA (1 ng/embryo). B: Animal caps were prepared from embryos injected with either untagged or mycGFP-Slug RNAs; both produced a similar increase in Sox9 RNA levels. C: Animal caps, from embryos injected with either mt-GFP or mycGFP-Slug RNAs, were analyzed when control embryos reached stage 11 or stage 16; at stage 11 mycGFP-Slug induced an increase in Sox9 RNA levels, which returned to baseline by stage 16. No change in Sox9 RNA levels were observed at either stage in animal caps expressing mt-GFP. In GR-Slug injected caps, levels of Bcl-xL (D), Sox9 (E), RelA, Rel2 and Rel3 (F), and caspase-9, -3 and -6 (G) RNAs were increased in response to dexamethasone; with the sole exception of caspase-9, these increases were blocked by emetine. In all panels, error bars reflect standard deviation from the mean of multiple experiments.doi:10.1371/journal.pone.0000106.g008
Caspase-9 encodes an initiator caspase involved in the maternal/early embryonic apoptotic program in X. laevis, while the effector caspases-3 and -6 act downstream . Caspase-9 appears to be a direct and negatively regulated target of Slug, while caspases-3 and -6 appear to be indirect targets (FIG. 8G). In embryos, Slug MO induced an increase in caspase activity as indicated by staining with the anti-activated caspase antibody CM1 and increased cleavage of a caspase-3 target peptide (data not shown). These results extend those of Tribulo et al.,  and establish, apparently for the first time, a direct regulatory interaction between Slug and caspase-9.
In analogy with polymerization reactions, scientific studies often involve distinct initiation and catalytic events. In this work, the initiator was the observation that anti-apoptotic proteins rescue the effects of blocking Slug expression on mesodermal and neural crest markers. Bcl-xL produced an increase in both Slug and Snail RNAs and Snail itself is sufficient to suppress the Slug morpholino phenotype (FIG. 1J–L). The role of catalyst was played by the observations of Kirshenbaum and colleagues [XPATH ERROR: unknown variable "start".]–, who found that Bcl-2 regulates NF-κB activity by activating IκB kinase (IκK). Activation of IκK induces the degradation of inhibitory IκB proteins, leading to increased NF-κB activity. Our studies using the dominant negative IκBsa protein, the IκK inhibitor AKBA, and the dominant negative form of RelA, RelAΔSP, indicate that Bcl-xL regulates Slug RNA expression via NF-κB in the early X. laevis embryo (FIG. 9).
Figure 9. Bcl-xL-Slug-NF-κB network:
This diagram focuses on the regulatory interactions uncovered in the course of our studies (see text for caveats associated with the identification of direct interactions). Protein names are underlined, gene names are in italics. Bcl-xL appears to activate NF-κB through effects on IκK activity and IκB stability. NF-κB acts directly to regulate Slug, Snail, RelA, and Rel3 levels; NF-κB regulation of the expression of its inhibitor IκB is based on data from mammalian systems. Caspase-9 was the only direct target of Slug identified in our studies; indirect interactions are indicated by dotted lines.doi:10.1371/journal.pone.0000106.g009
Mapping regulatory interactions
In mammalian epithelial cells over-expression of Bcl-2 has been found to promote EMT  and suppress cadherin expression . Neither study, however, examined the effects of Bcl-2 expression on the levels of Slug or Snail RNAs. NF-κB itself has been implicated in EMT , and has been found to regulate Snail stability and activity through effects on glycogen synthase kinase 3 . In X. laevis, few promoters have been rigorously defined. It is possible, however, to tentatively classify regulatory interactions as direct or indirect based on the ability of hormone-regulated proteins to influence target RNAs in the presence of protein synthesis inhibitors. If a regulatory interaction requires or depends upon on-going protein synthesis, it is classified as “indirect”; “direct” interaction are not blocked by protein synthesis inhibitors.
Regulatory interactions are often complex and multifaceted; it is important to remember that conclusions based on hormone-regulated proteins need to be characterized further. For example, if a transcription factor regulates the expression of a gene encoding a microRNA, which in turn regulates the stability of a target RNA, its effects will appear as direct even though they are mechanistically indirect. As another example, NF-κB acts as both a transcriptional regulator  and has been reported to destabilize certain RNAs . This latter activity would appear as direct in our system. In this light it is interesting to note that activation of GR-RelA leads to a protein-synthesis independent decrease in levels of p53 RNA (unpubl. obs); whether this reflects transcriptional, post-transcriptional, or microRNA-mediated regulation is not yet resolved.
Neither Bcl-2 or Bcl-xL are thought to regulate gene expression through direct interactions with DNA or transcription factors, but rather through effects on various kinases [see 74]–. Using a hormone-regulated form of Bcl-xL, RelA and Rel3 appear to be direct, while Rel2 and Slug appear to be indirect targets of Bcl-xL regulation. The ability of Bcl-xL to induce changes in RelA, Slug and Snail RNA levels is inhibited by both the dominant negative form of RelA, RelAΔSP and the IκK inhibitor AKBA, suggesting that Bcl-xL activates IκK, which initiates the destruction of IκB polypeptides, leading to the activation of pre-existing NF-κB, which in turn regulates target genes (FIG. 9). The presence of RelAΔSP or AKBA blocks these NF-κB-dependent processes and so blocks the effects of Bcl-xL on both “direct” and “indirect” targets.
NF-κB regulation of Slug
NF-κB subunit proteins are expressed ubiquitously and play a key role in cellular inflammation and tumor progression –. NF-κB is known to have a number of regulatory targets, including IκBα , , which acts to repress, and so limit, NF-κB activity. In mammalian systems, NF-κB regulates the expression of a range of anti-apoptotic proteins, including the anti-apoptotic caspase inhibitor proteins (IAPs), Bcl-2, and Bcl-xL – and decreases the activity of the pro-apoptotic p53 protein in renal cell carcinoma cells . In addition, NF-κB and p53 can inhibit each other's activities by competing for the limited pool of CBP/p300 within the cell . In X. laevis RelA/NF-κB is a positive regulator of Bcl-xL, Slug and Snail. Slug's ability to down-regulate the pro-apoptotic genes Caspase-9  and Puma  would be expected to generate an over-all anti-apoptotic state.
In this light, the decrease in NF-κB RNA levels at the midblastula transition (FIG. 5A) may be permissive in the regulation apoptotic processes in later stage embryos –. A number of drugs, e.g. AKBA and curcumin , , –, inhibit NF-κB activity and increase apoptosis, perhaps by reducing levels of Slug and/or Snail expression, which may explain at least part of their anti-tumor effects.
Slug regulation of NF-κB
In X. laevis Slug activates an NF-κB responsive reporter and acts indirectly to increase levels of RelA, Rel2, and Rel3 RNAs. In Drosophila Snail also acts indirectly to regulate Dorsal (RelA) by inhibiting expression of WntD, which acts to inhibit activation of Dorsal , . How Slug regulates RelA/Rel3 expression in Xenopus remains unclear, but preliminary studies indicate that Drosophila WntD, as well as a number of Xenopus Wnts, inhibit Bcl-xL-mediated activation of the 3XκB-Luc reporter and reduce RelA RNA levels (Zhang & Klymkowsky, unpubl. obs.). Whether Slug acts through the regulation of a Wnt or some other intermediate, it is apparent that Slug can increase NF-κB activity and RNA levels in the early Xenopus embryo. Our studies indicate that NF-κB regulates both Slug and Snail RNA levels and plays an essential role in mesoderm formation. The presence of a heretofore unrecognized NF-κB–Slug/Snail regulatory loop in a vertebrates should have important consequences for our understanding the conserved and divergent evolutionary mechanisms involved in germ layer specification, as well as practical implications for therapeutic interventions that target NF-κB and Slug/Snail-mediated EMT and anti-apoptotic processes.
Materials and Methods
Embryos and animal caps
X. laevis embryos were obtained following standard protocols ,  from adult animals purchased from Xenopus I, Inc. (Dexter, MI). Embryos were staged according to Nieuwkoop and Faber . Fertilized eggs or one-cell of two-cell embryos were injected with 10–20 nL of solution; ectodermal explants (animal caps) were prepared from stage 8/9 embryos using a Gastromaster™ (Xenotech) and cultured until control embryos reached either stage 11 or stage 16/17 , . In experiments involving hormone activation of chimeric polypeptides, whole embryos or animal caps were treated with 20 µM dexamethasone (Sigma) alone, or were pretreated for 30 minutes with 100 µg/mL of the protein synthesis inhibitor emetine (Sigma), prior to dexamethasone and emetine treatment. In contrast to cycloheximide , emetine does not induce nodal gene expression under these conditions , . RNA was isolated and subjected to either standard or quantitative RT-PCR (QRT-PCR) analysis as described previously . Primers for PCR analyses were:
caspase-3 [F5′AAGTCTGGAACATCGCAGG3′; R5′TAAATGAGCCCCTCATCACC3′];
caspase-6 [F5′TGGACATCAAGGACTGTGGA3′; R5′CTGAACATCAAACCCCAGGT 3′];
caspase-9 [F5′CCGATGGAGTTTCAAGCAAA3′; R5′GACTGGGCAGAAGGATTCAG3′];
Bcl-xL/Xr11 [F5′GTCGGCCTGTATGGAAAGAA3′; R5′CATGATAGGCGACCCAGTG3′] ;
Slug [F5′CAATGCAAGAACTGTTCC3′; R5′TCTAGGCAAGAATTGCTC3′];
Snail [F5′AAGCACAATGGACTCCTT3′; R5′CCAATAGTGATACACACC3′];
Rel2 [F5′GCAGTTCCATCACAGCTAAAC3′; R5′GGTGTCTGGTAGCCTTTGGTC3′];
Rel3 [F5′ATCATGGAAAGTTTGAGGGCA3′; R5′GGGTGGTAACTAAATGGTGTA3′];
RelB [F5′CCTCAGTACTGTAACTGTCGC3′; R5′GCAGTCTTTACCTACAAGGCC3′];
Xp100 [F5′CTGATAAACATGCCAGATTAC3′; R5′GCACATCAGAGTCACTCTCAG3′](this study.
Plasmids, morpholinos, and reporters
pCS2mycGFP-Slug and pCS2mt-GFP have been described previously . Plasmids encoding dexamethasone-regulated versions of Slug, RelA and Bcl-xL were generated by subcloning into the pCS2GR-Sox7-GFP plasmid . A plasmid encoding an epitope-tagged form of Xenopus Bcl-xL (Xr11) was supplied by James Maller (UCHSC, Denver, CO); the pCS2-LacZ plasmid was supplied by Jing Yang (Columbus Children's Research Institute); a plasmid encoding human Bcl-2 was supplied by Jean Gautier (Columbia University); plasmids encoding Xenopus RelA(Rel1), myc-tagged Rel3, Xp52 (the active form of Xp100), and a dominant-negative form of RelA, RelAΔSP, were supplied by Hugh Woodland (U. Warwick)Beck et al 1998), Ken Kao (U. Newfoundland), , , and Jun-ichiro Inoue (U. Tokyo), . The Xp52 sequence was subcloned into pCS2 to form pCS2-Xp52. The p3XκB-Luc plasmid, which contains three κB binding sites driving expression of firefly luciferase  and a plasmid encoding a form of human IκBα (IκBsa) in which serines 32 and 36 have been mutated to alanines  were supplied by Lorrie Kirshenbaum (U. Manitoba). IκBsa is resistant to IκB kinase phosphorylation and subsequent proteolytic degradation, and so acts as a dominant-negative regulator of NF-κB activity , . The coding sequence for X. laevis IκBα (GENBANK Accession AAH77876) was isolated by RT-PCR and subcloned into a pCS2-V5 plasmid to form pCS2-XIκBα-V5. Acetyl-11-keto-β-boswellic acid (AKBA), a pentacyclic triterpene, inhibits IκBα phosphorylation and degradation in mammalian systems –; it has also been reported to inhibit topoisomerases ,  and 5-lipoxygenase . The effects of AKBA on IκBα-V5 stability in Xenopus were analyzed using SDS-PAGE/immunoblot with an monoclonal anti-V5 epitope antibody (Invitrogen) and the antiSOX3c antibody . Reporter assays were carried out using the dual luciferase system . Capped RNAs were generated using Ambion mMessage mMachine kits. Both fluorescein-conjugated and unconjugated forms of a morpholino directed against the 5′ UTR and coding sequence of the SlugA and SlugB genes (FIG. 1A) [5′CGTGGCATTTTCACTGCGGGCGGGA3′] were used with identical results; these and a control morpholino were purchased from Gene Tools, Inc.
TUNEL, anti-caspase staining and caspase cleavage assays
Fixed and sectioned embryos  were stained by TdT-mediated dUTP-biotin nick end-labeling (TUNEL) using a peroxidase-based kit purchased from Molecular Probes, following the manufacturer's instructions. Whole-mount TUNEL  was carried out using the protocol on the Harland Lab website1. The rabbit anti-activated caspase 3 antibody CM1 (BD Bioscience Pharmingen) was used in whole-mount immunocytochemistry at a dilution of 1:1000 following standard immunocytochemical techniques , . For caspase cleavage assays, embryo lysates were prepared and reactions were carried out in duplicate using one embryo equivalent of lysate (20 µL) and 80 µL lysis buffer. Reactions were incubated at 37°C for 1 hour with 5 µM of the caspase-3 fluorogenic substrate Ac-DEVD-AMC (BioMol), after which 990 µL of water was added and fluorescence was measured using a Hitachi F2000 Fluorescence Spectrophotometer. Results were analyzed for statistical significance using Student's t-test of the means.
In situ hybridization and Alcian Blue Staining
Plasmids containing the Sox9 coding sequence, isolated by RT-PCR from neural stage embryos (Fawcett & Klymkowsky, unpublished). Digoxigenin-labeled antisense probes were generated against Sox9, Slug , Sox2 , Sox3 , epidermal keratin , Xbra , Antipodean (Apod) , and Xmenf  RNAs and in situ hybridization was performed following standard protocols , . Alcian Blue staining was carried out as described previously . Digital images were captured using a Nikon CoolPix 995 Camera on an Inverted Leica M400 Photomicroskop. Images were manipulated with Fireworks 8 software (Macromedia now Adobe) using the “auto levels” and “curves” functions only.
We thank all previous reviewers of this manuscript for their insights, which have improved the work; we also thank Lorrie Kirshenbaum, Tim Grammer, Hugh Woodland, Ken Kao, James Maller, Jean Gautier, Jan Christian, Randy Moon, Tom Sargent, Mary Lou King, Janet Heasman, Aaron Zorn, Michael Gordon, Roel Nusse, Anne-Hélène Monsoro-Burq, Carol LaBonne, and Jean-Pierre Saint-Jeannett for plasmids and helpful discussions; Tamara Basta for in vitro translation experiments; Jonathon Keeney for help in isolating Xenopus IκB; Leslie Leinwand, Norm Pace and William Eberle for use of equipment.
This manuscript is dedicated to Martin Raff, a constant inspiration and a friend to many.
Conceived and designed the experiments: MK CZ TC. Performed the experiments: MK CZ TC ET. Analyzed the data: MK CZ TC ET. Contributed reagents/materials/analysis tools: TS. Wrote the paper: MK CZ.
- 1. Thiery JP, Sleeman JP (2006) Complex networks orchestrate epithelial-mesenchymal transitions. Nat Rev Mol Cell Biol 7: 131–42. doi: 10.1038/nrm1835
- 2. Fritzenwanker JH, Saina M, Technau U (2004) Analysis of forkhead and snail expression reveals epithelial-mesenchymal transitions during embryonic and larval development of Nematostella vectensis. Dev Biol 275: 389–402. doi: 10.1016/j.ydbio.2004.08.014
- 3. Technau U, Scholz CB (2003) Origin and evolution of endoderm and mesoderm. Int J Dev Biol 47: 531–9.
- 4. Vickaryous MK, Hall BK (2006) Human cell type diversity, evolution, development, and classification with special reference to cells derived from the neural crest. Biol Rev Camb Philos Soc 81: 425–55. doi: 10.1017/S1464793106007068
- 5. Whiteley M, Noguchi PD, Sensabaugh SM, Odenwald WF, Kassis JA (1992) The Drosophila gene escargot encodes a zinc finger motif found in snail-related genes. Mech Dev 36: 117–27. doi: 10.1016/0925-4773(92)90063-P
- 6. Ashraf SI, Hu X, Roote J, Ip YT (1999) The mesoderm determinant snail collaborates with related zinc-finger proteins to control Drosophila neurogenesis. EMBO J 18: 6426–38. doi: 10.1093/emboj/18.22.6426
- 7. Cai Y, Chia W, Yang X (2001) A family of snail-related zinc finger proteins regulates two distinct and parallel mechanisms that mediate Drosophila neuroblast asymmetric divisions. EMBO J 20: 1704–14. doi: 10.1093/emboj/20.7.1704
- 8. Roark M, Sturtevant MA, Emery J, Vaessin H, Grell E, Bier E (1995) scratch, a pan-neural gene encoding a zinc finger protein related to snail, promotes neuronal development. Genes Dev 9: 2384–98. doi: 10.1101/gad.9.19.2384
- 9. Manzanares M, Locascio A, Nieto MA (2001) The increasing complexity of the Snail gene superfamily in metazoan evolution. Trends Genet 17: 178–81. doi: 10.1016/S0168-9525(01)02232-6
- 10. Lespinet O, Nederbragt AJ, Cassan M, Dictus WJ, van Loon AE, et al. (2002) Characterisation of two snail genes in the gastropod mollusc Patella vulgata. Implications for understanding the ancestral function of the snail-related genes in Bilateria. Dev Genes Evol 212: 186–95. doi: 10.1007/s00427-002-0228-1
- 11. Grau Y, Carteret C, Simpson P (1984) Mutations and chromosomal rearrangements affecting the expression of Snail, a gene involved in embryonic patterning in Drosophila melanogaster. Genetics 108: 347–360.
- 12. Simpson P (1983) Maternal-zygotic gene interactions during formation of the dorsoventral patternin in Drosophila embryos. Genetics 105: 615–632.
- 13. Nusslein-Volhard C, Wieschaus E, Kluding H (1984) Mutations afffecting the pattern of the larval cuticle in Drosophila melanogaster. I. Zygotic loci on the second chromosome. Roux's Arch Dev Biol 193: 267–282. doi: 10.1007/bf00848156
- 14. Carver EA, Jiang R, Lan Y, Oram KF, Gridley T (2001) The mouse snail gene encodes a key regulator of the epithelial-mesenchymal transition. Mol Cell Biol 21: 8184–8. doi: 10.1128/MCB.21.23.8184-8188.2001
- 15. Jiang R, Lan Y, Norton CR, Sundberg JP, Gridley T (1998) The Slug gene is not essential for mesoderm or neural crest development in mice. Dev Biol 198: 277–85. doi: 10.1006/dbio.1998.8909
- 16. Murray SA, Gridley T (2006) Snail family genes are required for left-right asymmetry determination, but not neural crest formation, in mice. Proc Natl Acad Sci U S A 103: 10300–4. doi: 10.1073/pnas.0602234103
- 17. Sanchez-Martin M, Perez-Losada J, Rodriguez-Garcia A, Gonzalez-Sanchez B, Korf BR, et al. (2003) Deletion of the SLUG (SNAI2) gene results in human piebaldism. Am J Med Genet A 122: 125–32. doi: 10.1002/ajmg.a.20345
- 18. Perez-Losada J, Sanchez-Martin M, Rodriguez-Garcia A, Sanchez ML, Orfao A, et al. (2002) Zinc-finger transcription factor Slug contributes to the function of the stem cell factor c-kit signaling pathway. Blood 100: 1274–86.
- 19. Savagner P, Kusewitt DF, Carver EA, Magnino F, Choi C, et al. (2005) Developmental transcription factor slug is required for effective re-epithelialization by adult keratinocytes. J Cell Physiol 202: 858–66. doi: 10.1002/jcp.20188
- 20. Nieto MA, Sargent MG, Wilkinson DG, Cooke J (1994) Control of cell behavior during vertebrate development by Slug, a zinc finger gene. Science 264: 835–9. doi: 10.1126/science.7513443
- 21. Mayor R, Guerrero N, Young RM, Gomez-Skarmeta JL, Cuellar C (2000) A novel function for the Xslug gene: control of dorsal mesendoderm development by repressing BMP-4. Mech Dev 97: 47–56. doi: 10.1016/S0925-4773(00)00412-3
- 22. Carl TF, Dufton C, Hanken J, Klymkowsky MW (1999) Inhibition of Neural Crest Migration in Xenopus Using Antisense Slug RNA. Dev Biol 213: 101–115. doi: 10.1006/dbio.1999.9320
- 23. Aybar MJ, Nieto MA, Mayor R (2003) Snail precedes slug in the genetic cascade required for the specification and migration of the Xenopus neural crest. Development 130: 483–94. doi: 10.1242/dev.00238
- 24. LaBonne C, Bronner-Fraser M (2000) Snail-related transcriptional repressors are required in Xenopus for both the induction of the neural crest and its subsequent migration. Dev Biol 221: 195–205. doi: 10.1006/dbio.2000.9609
- 25. Sefton M, Sanchez S, Nieto MA (1998) Conserved and divergent roles for members of the Snail family of transcription factors in the chick and mouse embryo. Development 125: 3111–21.
- 26. Locascio A, Manzanares M, Blanco MJ, Nieto MA (2002) Modularity and reshuffling of Snail and Slug expression during vertebrate evolution. Proc Natl Acad Sci USA 99: 16841–6. doi: 10.1073/pnas.262525399
- 27. Sakai D, Suzuki T, Osumi N, Wakamatsu Y (2006) Cooperative action of Sox9, Snail2 and PKA signaling in early neural crest development. Development 133: 1323–33. doi: 10.1242/dev.02297
- 28. Hemavathy K, Ashraf SI, Ip YT (2000) Snail/Slug family of repressors: slowly going into the fast lane of development and cancer. Gene 257: 1–12. doi: 10.1016/S0378-1119(00)00371-1
- 29. Jiang J, Levine M (1993) Binding affinities and cooperative interactions with bHLH activators delimit threshold responses to the dorsal gradient morphogen. Cell 72: 741–52. doi: 10.1016/0092-8674(93)90402-C
- 30. Thellmann M, Hatzold J, Conradt B (2003) The Snail-like CES-1 protein of C. elegans can block the expression of the BH3-only cell-death activator gene egl-1 by antagonizing the function of bHLH proteins. Development 130: 4057–71. doi: 10.1242/dev.00597
- 31. Nakakura EK, Watkins DN, Schuebel KE, Sriuranpong V, Borges MW, et al. (2001) Mammalian Scratch: a neural-specific Snail family transcriptional repressor. Proc Natl Acad Sci USA 98: 4010–5. doi: 10.1073/pnas.051014098
- 32. Savagner P, Yamada KM, Thiery JP (1997) The zinc-finger protein slug causes desmosome dissociation, an initial and necessary step for growth factor-induced epithelial-mesenchymal transition. J Cell Biol 137: 1403–19. doi: 10.1083/jcb.137.6.1403
- 33. Kurrey NK, Amit K, Bapat SA (2005) Snail and Slug are major determinants of ovarian cancer invasiveness at the transcription level. Gynecol Oncol 97: 155–65. doi: 10.1016/j.ygyno.2004.12.043
- 34. Cano A, Perez MM, Rodrigo I, Locascio A, Blanco MJ, et al. (2000) The transcription factor Snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression. Nat Cell Biol 2: 76–83. doi: 10.1038/35000025
- 35. Batlle E, Sancho E, Franci C, Dominguez D, Monfar M, Baulida J, Garcia De Herreros A (2000) The transcription factor Snail is a repressor of E-cadherin gene expression in epithelial tumour cells. Nat Cell Biol 2: 84–89. doi: 10.1038/35000034
- 36. Poser I, Dominguez D, de Herreros AG, Varnai A, Buettner R, et al. (2001) Loss of E-cadherin expression in melanoma cells involves up-regulation of the transcriptional repressor Snail. J Biol Chem 276: 24661–6. doi: 10.1074/jbc.M011224200
- 37. Ikenouchi J, Matsuda M, Furuse M, Tsukita S (2003) Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail. J Cell Sci 116: 1959–67. doi: 10.1242/jcs.00389
- 38. Bolos V, Peinado H, Perez-Moreno MA, Fraga MF, Esteller M, et al. (2003) The transcription factor Slug represses E-cadherin expression and induces epithelial to mesenchymal transitions: a comparison with Snail and E47 repressors. J Cell Sci 116: 499–511. doi: 10.1242/jcs.00224
- 39. Hajra KM, Chen DY, Fearon ER (2002) The SLUG zinc-finger protein represses E-cadherin in breast cancer. Cancer Res 62: 1613–8.
- 40. Metzstein MM, Horvitz HR (1999) The C. elegans cell death specification gene ces-1 encodes a snail family zinc finger protein. Mol Cell 4: 309–19. doi: 10.1016/S1097-2765(00)80333-0
- 41. Inukai T, Inoue A, Kurosawa H, Goi K, Shinjyo T, et al. (1999) SLUG, a ces-1-related zinc finger transcription factor gene with antiapoptotic activity, is a downstream target of the E2A-HLF oncoprotein. Mol Cell 4: 343–52. doi: 10.1016/S1097-2765(00)80336-6
- 42. Inoue A, Seidel MG, Wu W, Kamizono S, Ferrando AA, et al. (2002) Slug, a highly conserved zinc finger transcriptional repressor, protects hematopoietic progenitor cells from radiation-induced apoptosis in vivo. Cancer Cell 2: 279–88. doi: 10.1016/S1535-6108(02)00155-1
- 43. Kajita M, McClinic KN, Wade PA (2004) Aberrant expression of the transcription factors snail and slug alters the response to genotoxic stress. Mol Cell Biol 24: 7559–66. doi: 10.1128/MCB.24.17.7559-7566.2004
- 44. Vega S, Morales AV, Ocana OH, Valdes F, Fabregat I, Nieto MA (2004) Snail blocks the cell cycle and confers resistance to cell death. Genes Dev 18: 1131–43. doi: 10.1101/gad.294104
- 45. Wu WS, Heinrichs S, Xu D, Garrison SP, Zambetti GP, et al. (2005) Slug Antagonizes p53-Mediated Apoptosis of Hematopoietic Progenitors by Repressing puma. Cell 123: 641–53. doi: 10.1016/j.cell.2005.09.029
- 46. Gupta PB, Kuperwasser C, Brunet JP, Ramaswamy S, Kuo WL, et al. (2005) The melanocyte differentiation program predisposes to metastasis after neoplastic transformation. Nat Genet 37: 1047–54. doi: 10.1038/ng1634
- 47. Shih JY, Tsai MF, Chang TH, Chang YL, Yuan A, et al. (2005) Transcription repressor slug promotes carcinoma invasion and predicts outcome of patients with lung adenocarcinoma. Clin Cancer Res 11: 8070–8. doi: 10.1158/1078-0432.CCR-05-0687
- 48. Come C, Arnoux V, Bibeau F, Savagner P (2004) Roles of the transcription factors snail and slug during mammary morphogenesis and breast carcinoma progression. J Mammary Gland Biol Neoplasia 9: 183–93. doi: 10.1023/B:JOMG.0000037161.91969.de
- 49. Perez-Mancera PA, Gonzalez-Herrero I, Perez-Caro M, Gutierrez-Cianca N, Flores T, et al. (2005) SLUG in cancer development. Oncogene 24: 3073–82. doi: 10.1038/sj.onc.1208505
- 50. Barrallo-Gimeno A, Nieto MA (2005) The Snail genes as inducers of cell movement and survival: implications in development and cancer. Development 132: 3151–61. doi: 10.1242/dev.01907
- 51. del Barrio MG, Nieto MA (2002) Overexpression of Snail family members highlights their ability to promote chick neural crest formation. Development 129: 1583–93.
- 52. Cheung M, Chaboissier MC, Mynett A, Hirst E, Schedl A, et al. (2005) The transcriptional control of trunk neural crest induction, survival, and delamination. Dev Cell 8: 179–92. doi: 10.1016/j.devcel.2004.12.010
- 53. Hemavathy K, Hu X, Ashraf SI, Small SJ, Ip YT (2004) The repressor function of snail is required for Drosophila gastrulation and is not replaceable by Escargot or Worniu. Dev Biol 269: 411–20. doi: 10.1016/j.ydbio.2004.01.029
- 54. Catalano A, Rodilossi S, Rippo MR, Caprari P, Procopio A (2004) Induction of stem cell factor/c-Kit/slug signal transduction in multidrug-resistant malignant mesothelioma cells. J Biol Chem 279: 46706–14. doi: 10.1074/jbc.M406696200
- 55. Moreno-Bueno G, Cubillo E, Sarrio D, Peinado H, Rodriguez-Pinilla SM, et al. (2006) Genetic profiling of epithelial cells expressing e-cadherin repressors reveals a distinct role for snail, slug, and e47 factors in epithelial-mesenchymal transition. Cancer Res 66: 9543–56. doi: 10.1158/0008-5472.CAN-06-0479
- 56. Zhou BP, Deng J, Xia W, Xu J, Li YM, et al. (2004) Dual regulation of Snail by GSK-3beta-mediated phosphorylation in control of epithelial-mesenchymal transition. Nat Cell Biol 6: 931–40. doi: 10.1038/ncb1173
- 57. Yook JI, Li XY, Ota I, Fearon ER, Weiss SJ (2005) Wnt-dependent regulation of the E-cadherin repressor snail. J Biol Chem 280: 11740–8. doi: 10.1074/jbc.M413878200
- 58. Yang Z, Rayala S, Nguyen D, Vadlamudi RK, Chen S, et al. (2005) Pak1 phosphorylation of snail, a master regulator of epithelial-to-mesenchyme transition, modulates snail's subcellular localization and functions. Cancer Res 65: 3179–84. doi: 10.1158/0008-5472.CAN-04-3480
- 59. Dominguez D, Montserrat-Sentis B, Virgos-Soler A, Guaita S, Grueso J, et al. (2003) Phosphorylation regulates the subcellular location and activity of the snail transcriptional repressor. Mol Cell Biol 23: 5078–89. doi: 10.1128/MCB.23.14.5078-5089.2003
- 60. Vernon AE, LaBonne C (2006) Slug stability is dynamically regulated during neural crest development by the F-box protein Ppa. Development 133: 3359–70. doi: 10.1242/dev.02504
- 61. Tribulo C, Aybar MJ, Sanchez SS, Mayor R (2004) A balance between the anti-apoptotic activity of Slug and the apoptotic activity of msx1 is required for the proper development of the neural crest. Dev Biol 275: 325–42. doi: 10.1016/j.ydbio.2004.07.041
- 62. Mayor R, Young R, Vargas A (1999) Development of neural crest in Xenopus. Curr Top Dev Biol 43: 85–113.
- 63. Vallin J, Thuret R, Giacomello E, Faraldo MM, Thiery JP, et al. (2001) Cloning and characterization of three Xenopus Slug promoters reveal direct regulation by Lef/beta-catenin signaling. J Biol Chem 287: 30350–8. doi: 10.1074/jbc.M103167200
- 64. Spokony RF, Aoki Y, Saint-Germain N, Magner-Fink E, Saint-Jeannet JP (2002) The transcription factor Sox9 is required for cranial neural crest development in Xenopus. Development 129: 421–32.
- 65. Saint-Germain N, Lee YH, Zhang Y, Sargent TD, Saint-Jeannet JP (2004) Specification of the otic placode depends on Sox9 function in Xenopus. Development 131: 1755–63. doi: 10.1242/dev.01066
- 66. Kengaku M, Okamoto H (1993) Basic fibroblast growth factor induces differentiation of neural tube and neural crest lineages of cultured ectoderm cells from Xenopus gastrula. Development 119: 1067–78.
- 67. Baker CV, Bronner-Fraser M (1997) The origins of the neural crest. Part I: embryonic induction. Mech Dev 69: 3–11. doi: 10.1016/S0925-4773(97)00132-9
- 68. Bonstein L, Elias S, Frank D (1998) Paraxial-fated mesoderm is required for neural crest induction in Xenopus embryos. Dev Biol 193: 156–68. doi: 10.1006/dbio.1997.8795
- 69. Monsoro-Burq AH, Fletcher RB, Harland RM (2003) Neural crest induction by paraxial mesoderm in Xenopus embryos requires FGF signals. Development 130: 3111–24. doi: 10.1242/dev.00531
- 70. Mayor R, Morgan R, Sargent MG (1995) Induction of the prospective neural crest of Xenopus. Development 121: 767–77.
- 71. Kolm PJ, Sive HL (1995) Efficient hormone-inducible protein function in Xenopus laevis. Dev Biol 171: 267–72. doi: 10.1006/dbio.1995.1279
- 72. Sive HL, Grainger RM, Harland RM (2000) Early development of Xenopus laevis: a laboratory manual. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
- 73. de Moissac D, Mustapha S, Greenberg AH, Kirshenbaum LA (1998) Bcl-2 activates the transcription factor NFkappaB through the degradation of the cytoplasmic inhibitor IkappaBalpha. J Biol Chem 273: 23946–51. doi: 10.1074/jbc.273.37.23946
- 74. Kirshenbaum LA (2000) Bcl-2 intersects the NFkappaB signalling pathway and suppresses apoptosis in ventricular myocytes. Clin Invest Med 23: 322–30.
- 75. Regula KM, Ens K, Kirshenbaum LA (2002) IKK beta is required for Bcl-2-mediated NF-kappa B activation in ventricular myocytes. J Biol Chem 277: 38676–82. doi: 10.1074/jbc.M206175200
- 76. Feng H, Xiang H, Mao YW, Wang J, Liu JP, et al. (2004) Human Bcl-2 activates ERK signaling pathway to regulate activating protein-1, lens epithelium-derived growth factor and downstream genes. Oncogene 23: 7310–21. doi: 10.1038/sj.onc.1208041
- 77. Trisciuoglio D, Iervolino A, Candiloro A, Fibbi G, Fanciulli M, et al. (2004) bcl-2 induction of urokinase plasminogen activator receptor expression in human cancer cells through Sp1 activation: involvement of ERK1/ERK2 activity. J Biol Chem 279: 6737–45. doi: 10.1074/jbc.M308938200
- 78. Brockman JA, Scherer DC, McKinsey TA, Hall SM, Qi X, et al. (1995) Coupling of a signal response domain in I kappa B alpha to multiple pathways for NF-kappa B activation. Mol Cell Biol 15: 2809–18.
- 79. Syrovets T, Gschwend JE, Buchele B, Laumonnier Y, Zugmaier W, et al. (2005) Inhibition of IkappaB kinase activity by acetyl-boswellic acids promotes apoptosis in androgen-independent PC-3 prostate cancer cells in vitro and in vivo. J Biol Chem 280: 6170–80. doi: 10.1074/jbc.M409477200
- 80. Syrovets T, Buchele B, Krauss C, Laumonnier Y, Simmet T (2005) Acetyl-boswellic acids inhibit lipopolysaccharide-mediated TNF-alpha induction in monocytes by direct interaction with IkappaB kinases. J Immunol 174: 498–506.
- 81. Takada Y, Ichikawa H, Badmaev V, Aggarwal BB (2006) Acetyl-11-keto-beta-boswellic acid potentiates apoptosis, inhibits invasion, and abolishes osteoclastogenesis by suppressing NF-kappa B and NF-kappa B-regulated gene expression. J Immunol 176: 3127–40.
- 82. Kao KR, Hopwood ND (1991) Expression of a mRNA related to c-rel and dorsal in early Xenopus laevis embryos. Proc Natl Acad Sci USA 88: 2697–701. doi: 10.1073/pnas.88.7.2697
- 83. Richardson JC, Garcia EA, Woodland HR (1994) XrelA, a Xenopus maternal and zygotic homologue of the p65 subunit of NF-kappa B. Characterisation of transcriptional properties in the developing embryo and identification of a negative interference mutant. Mech Dev 45: 173–89. doi: 10.1016/0925-4773(94)90031-0
- 84. Tannahill D, Wardle FC (1995) Control of axis formation in Xenopus by the NF-kappa B-I kappa B system. Int J Dev Biol 39: 549–58.
- 85. Lake BB, Ford R, Kao KR (2001) Xrel3 is required for head development in Xenopus laevis. Development 128: 263–73.
- 86. Suzuki K, Yamamoto T, Inoue J (1995) Molecular cloning of cDNA encoding the Xenopus homolog of mammalian RelB. Nucleic Acids Res 23: 4664–9. doi: 10.1093/nar/23.22.4664
- 87. Suzuki K, Tsuchida J, Yamamoto T, Inoue J (1998) Identification and expression of the Xenopus homolog of mammalian p100-NFkappaB2. Gene 206: 1–9. doi: 10.1016/S0378-1119(97)00561-1
- 88. Cruz-Reyes J, Tata JR (1995) Cloning, characterization and expression of two Xenopus bcl-2-like cell-survival genes. Gene 158: 171–9. doi: 10.1016/0378-1119(95)00159-4
- 89. Beck CW, Sutherland DJ, Woodland HR (1998) Involvement of NF-kappaB associated proteins in FGF-mediated mesoderm induction. Int J Dev Biol 42: 67–77.
- 90. Ganguly A, Jiang J, Ip YT (2005) Drosophila WntD is a target and an inhibitor of the Dorsal/Twist/Snail network in the gastrulating embryo. Development 132: 3419–29. doi: 10.1242/dev.01903
- 91. Gordon MD, Dionne MS, Schneider DS, Nusse R (2005) WntD is a feedback inhibitor of Dorsal/NF-kappaB in Drosophila development and immunity. Nature 437: 746–9. doi: 10.1038/nature04073
- 92. Nakata K, Koyabu Y, Aruga J, Mikoshiba K (2000) A novel member of the Xenopus Zic family, Zic5, mediates neural crest development. Mech Dev 99: 83–91. doi: 10.1016/S0925-4773(00)00480-9
- 93. Meulemans D, Bronner-Fraser M (2004) Gene-regulatory interactions in neural crest evolution and development. Dev Cell 7: 291–9. doi: 10.1016/j.devcel.2004.08.007
- 94. Takayama E, Higo T, Kai M, Fukasawa M, Nakajima K, et al. (2004) Involvement of caspase-9 in execution of the maternal program of apoptosis in Xenopus late blastulae overexpressed with S-adenosylmethionine decarboxylase. Biochem Biophys Res Commun 325: 1367–75. doi: 10.1016/j.bbrc.2004.10.179
- 95. de Moissac D, Zheng H, Kirshenbaum LA (1999) Linkage of the BH4 domain of Bcl-2 and the nuclear factor kappaB signaling pathway for suppression of apoptosis. J Biol Chem 274: 29505–9. doi: 10.1074/jbc.274.41.29505
- 96. Lu PJ, Lu QL, Rughetti A, Taylor-Papadimitriou J (1995) bcl-2 overexpression inhibits cell death and promotes the morphogenesis, but not tumorigenesis of human mammary epithelial cells. J Cell Biol 129: 1363–78. doi: 10.1083/jcb.129.5.1363
- 97. Li L, Backer J, Wong AS, Schwanke EL, Stewart BG, et al. (2003) Bcl-2 expression decreases cadherin-mediated cell-cell adhesion. J Cell Sci 116: 3687–700. doi: 10.1242/jcs.00644
- 98. Huber MA, Azoitei N, Baumann B, Grunert S, Sommer A, et al. (2004) NF-kappaB is essential for epithelial-mesenchymal transition and metastasis in a model of breast cancer progression. J Clin Invest 114: 569–81. doi: 10.1172/JCI21358
- 99. Bachelder RE, Yoon SO, Franci C, de Herreros AG, Mercurio AM (2005) Glycogen synthase kinase-3 is an endogenous inhibitor of Snail transcription: implications for the epithelial-mesenchymal transition. J Cell Biol 168: 29–33. doi: 10.1083/jcb.200409067
- 100. Jiang J, Rushlow CA, Zhou Q, Small S, Levine M (1992) Individual dorsal morphogen binding sites mediate activation and repression in the Drosophila embryo. EMBO J 11: 3147–54.
- 101. Sitcheran R, Cogswell PC, Baldwin AS Jr (2003) NF-kappaB mediates inhibition of mesenchymal cell differentiation through a posttranscriptional gene silencing mechanism. Genes Dev 17: 2368–73. doi: 10.1101/gad.1114503
- 102. Gilmore T, Gapuzan ME, Kalaitzidis D, Starczynowski D (2002) Rel/NF-kappa B/I kappa B signal transduction in the generation and treatment of human cancer. Cancer Lett 181: 1–9. doi: 10.1016/S0304-3835(01)00795-9
- 103. Campbell KJ, Perkins ND (2006) Regulation of NF-kappaB function. Biochem Soc Symp 73: 165–80.
- 104. Dobrovolskaia MA, Kozlov SV (2005) Inflammation and cancer: when NF-kappaB amalgamates the perilous partnership. Curr Cancer Drug Targets 5: 325–44. doi: 10.2174/1568009054629645
- 105. Karin M (2006) Nuclear factor-kappaB in cancer development and progression. Nature 441: 431–6. doi: 10.1038/nature04870
- 106. Pikarsky E, Porat RM, Stein I, Abramovitch R, Amit S, et al. (2004) NF-kappaB functions as a tumour promoter in inflammation-associated cancer. Nature 431: 461–6. doi: 10.1038/nature02924
- 107. Read MA, Whitley MZ, Williams AJ, Collins T (1994) NF-kappa B and I kappa B alpha: an inducible regulatory system in endothelial activation. J Exp Med 179: 503–12. doi: 10.1084/jem.179.2.503
- 108. Ito CY, Kazantsev AG, Baldwin AS Jr (1994) Three NF-kappa B sites in the I kappa B-alpha promoter are required for induction of gene expression by TNF alpha. Nucleic Acids Res 22: 3787–92. doi: 10.1093/nar/22.18.3787
- 109. Tsukahara T, Kannagi M, Ohashi T, Kato H, Arai M, Nunez G, et al. (1999) Induction of Bcl-x(L) expression by human T-cell leukemia virus type 1 Tax through NF-kappaB in apoptosis-resistant T-cell transfectants with Tax. J Virol 73: 7981–7.
- 110. Khoshnan A, Tindell C, Laux I, Bae D, Bennett B, et al. (2000) The NF-kappa B cascade is important in Bcl-xL expression and for the anti-apoptotic effects of the CD28 receptor in primary human CD4+ lymphocytes. J Immunol 165: 1743–54.
- 111. Pise-Masison CA, Mahieux R, Jiang H, Ashcroft M, Radonovich M, et al. (2000) Inactivation of p53 by human T-cell lymphotropic virus type 1 Tax requires activation of the NF-kappaB pathway and is dependent on p53 phosphorylation. Mol Cell Biol 20: 3377–86. doi: 10.1128/MCB.20.10.3377-3386.2000
- 112. Kucharczak J, Simmons MJ, Fan Y, Gelinas C (2003) To be, or not to be: NF-kappaB is the answer–role of Rel/NF-kappaB in the regulation of apoptosis. Oncogene 22: 8961–82. doi: 10.1038/sj.onc.1207230
- 113. Li Q, Verma IM (2002) NF-kappaB regulation in the immune system. Nat Rev Immunol 2: 725–34. doi: 10.1038/nri910
- 114. Gurova KV, Hill JE, Guo C, Prokvolit A, Burdelya LG, et al. (2005) Small molecules that reactivate p53 in renal cell carcinoma reveal a NF-kappaB-dependent mechanism of p53 suppression in tumors. Proc Natl Acad Sci USA 102: 17448–53. doi: 10.1073/pnas.0508888102
- 115. Webster GA, Perkins ND (1999) Transcriptional cross talk between NF-kappaB and p53. Mol Cell Biol 19: 3485–95.
- 116. Finkielstein CV, Lewellyn AL, Maller JL (2001) The midblastula transition in Xenopus embryos activates multiple pathways to prevent apoptosis in response to DNA damage. Proc Natl Acad Sci USA 98: 1006–11. doi: 10.1073/pnas.98.3.1006
- 117. Stack JH, Newport JW (1997) Developmentally regulated activation of apoptosis early in Xenopus gastrulation results in cyclin A degradation during interphase of the cell cycle. Development 124: 3185–95.
- 118. Hensey C, Gautier J (1997) A developmental timer that regulates apoptosis at the onset of gastrulation. Mech Dev 69: 183–95. doi: 10.1016/S0925-4773(97)00191-3
- 119. Li L, Aggarwal BB, Shishodia S, Abbruzzese J, Kurzrock R (2004) Nuclear factor-kappaB and IkappaB kinase are constitutively active in human pancreatic cells, and their down-regulation by curcumin (diferuloylmethane) is associated with the suppression of proliferation and the induction of apoptosis. Cancer 101: 2351–62. doi: 10.1002/cncr.20605
- 120. Su CC, Chen GW, Lin JG, Wu LT, Chung JG (2006) Curcumin inhibits cell migration of human colon cancer colo 205 cells through the inhibition of nuclear factor kappa B /p65 and down-regulates cyclooxygenase-2 and matrix metalloproteinase-2 expressions. Anticancer Res 26: 1281–8.
- 121. Sandur SK, Ichikawa H, Sethi G, Ahn KS, Aggarwal BB (2006) Plumbagin (5-Hydroxy-2-methyl-1,4-naphthoquinone) Suppresses NF-kappaB Activation and NF-kappaB-regulated Gene Products Through Modulation of p65 and IkappaBalpha Kinase Activation, Leading to Potentiation of Apoptosis Induced by Cytokine and Chemotherapeutic Agents. J Biol Chem 281: 17023–33. doi: 10.1074/jbc.M601595200
- 122. Nieuwkoop PD, Faber J (1967) Normal Table of Xenopus laevis (Daudin). Amsterdam: North-Holland Publishing Company.
- 123. Zhang C, Basta T, Jensen ED, Klymkowsky MW (2003) The beta-catenin/VegT-regulated early zygotic gene Xnr5 is a direct target of SOX3 regulation. Development 130: 5609–24. doi: 10.1242/dev.00798
- 124. Entner N, Grollman AP (1973) Inhibition of protein synthesis: a mechanism of amebicide action of emetine and other structurally related compounds. J Protozool 20: 160–3. doi: 10.1111/j.1550-7408.1973.tb06025.x
- 125. Sinner D, Rankin S, Lee M, Zorn AM (2004) Sox17 and beta-catenin cooperate to regulate the transcription of endodermal genes. Development 131: 3069–3080. doi: 10.1242/dev.01176
- 126. Zhang C, Basta T, Klymkowsky MW (2005) SOX7 and SOX18 are essential for cardiogenesis in Xenopus. Dev Dyn 234: 878–891. doi: 10.1002/dvdy.20565
- 127. Zhang C, Basta T, Fawcett SR, Klymkowsky MW (2005) SOX7 is an immediate-early target of VegT and regulates Nodal expression in Xenopus. Dev Biol 278: 526–41. doi: 10.1016/j.ydbio.2004.11.008
- 128. Kao KR, Lockwood A (1996) Negative regulation of dorsal patterning in early embryos by overexpression of XrelA, a Xenopus homologue of NF-kappa B. Mech Dev 58: 129–39. doi: 10.1016/S0925-4773(96)00565-5
- 129. Yang S, Lockwood A, Hollett P, Ford R, Kao K (1998) Overexpression of a novel Xenopus rel mRNA gene induces tumors in early embryos. J Biol Chem 273: 13746–52. doi: 10.1074/jbc.273.22.13746
- 130. Wahl C, Liptay S, Adler G, Schmid RM (1998) Sulfasalazine: a potent and specific inhibitor of nuclear factor kappa B. J Clin Invest 101: 1163–74. doi: 10.1172/JCI992
- 131. Syrovets T, Buchele B, Gedig E, Slupsky JR, Simmet T (2000) Acetyl-boswellic acids are novel catalytic inhibitors of human topoisomerases I and IIalpha. Mol Pharmacol 58: 71–81.
- 132. Hoernlein RF, Orlikowsky T, Zehrer C, Niethammer D, Sailer ER, et al. (1999) Acetyl-11-keto-beta-boswellic acid induces apoptosis in HL-60 and CCRF-CEM cells and inhibits topoisomerase I. J Pharmacol Exp Ther 288: 613–9.
- 133. Safayhi H, Sailer ER, Ammon HP (1995) Mechanism of 5-lipoxygenase inhibition by acetyl-11-keto-beta-boswellic acid. Mol Pharmacol 47: 1212–6.
- 134. Carl T, Klymkowsky MW (1999) Whole-mount visualization of endogenous and exogenous proteins in Xenopus and other organisms. In: Ritcher J., editor. A comparative methods approach to the study of oocytes and embryos. New York: Oxford University Press. pp. 291–315.
- 135. Hensey C, Gautier J (1998) Programmed cell death during Xenopus development: a spatio-temporal analysis. Dev Biol 203: 36–48. doi: 10.1006/dbio.1998.9028
- 136. Yeo W, Gautier J (2004) Early neural cell death: dying to become neurons. Dev Biol 274: 233–44. doi: 10.1016/j.ydbio.2004.07.026
- 137. Dent JA, Polson AG, Klymkowsky MW (1989) A whole-mount immunocytochemical analysis of the expression of the intermediate filament protein vimentin in Xenopus. Development 105: 61–74.
- 138. Kishi M, Mizuseki K, Sasai N, Yamazaki H, Shiota K, et al. (2000) Requirement of Sox2-mediated signaling for differentiation of early Xenopus neuroectoderm. Development 127: 791–800.
- 139. Penzel R, Oschwald R, Chen Y, Tacke L, Grunz H (1997) Characterization and early embryonic expression of a neural specific transcription factor xSOX3 in Xenopus laevis. Int J Dev Biol 41: 667–77.
- 140. Jonas E, Sargent TD, Dawid IB (1985) Epidermal keratin gene expressed in embryos of Xenopus laevis. Proc Natl Acad Sci U S A 82(16): 5413–7. doi: 10.1073/pnas.82.16.5413
- 141. Smith JC, Price BM, Green JB, Weigel D, Herrmann BG (1991) Expression of a Xenopus homolog of Brachyury (T) is an immediate-early response to mesoderm induction. Cell 67: 79–87. doi: 10.1016/0092-8674(91)90573-H
- 142. Stennard F, Zorn AM, Ryan K, Garrett N, Gurdon JB (1999) Differential expression of VegT and Antipodean protein isoforms in Xenopus. Mech Dev 86: 87–98. doi: 10.1016/S0925-4773(99)00119-7
- 143. Kumano G, Smith WC (2002) The nodal target gene Xmenf is a component of an FGF-independent pathway of ventral mesoderm induction in Xenopus. Mech Dev 118: 45–56. doi: 10.1016/S0925-4773(02)00186-7