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Correction: Visualization of Glutamine Transporter Activities in Living Cells Using Genetically Encoded Glutamine Sensors

  • Katrin Gruenwald,
  • John Todd Holland,
  • Verlyn Stromberg,
  • Altaf Ahmad,
  • Daisy Watcharakichkorn,
  • Sakiko Okumoto
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The seventh sentence of the first paragraph under the subheading “Affinity and Substrate Specificities of FLIPQ-TV3.0 Sensors” of the Results and Discussion section is incorrect. The correct sentence is: The resulting clones, FLIPQ-TV3.0_R75K, R75M, D157N, R75MW220A, and R75MY86A had Kd of 1.5×10−6 M, 5.3×10−5 M, 1.3×10−4 M, and 1.6×10−3, and 7.6×10−3 M, respectively (Fig 4A, Table 1).

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Fig 4. Affinities and substrate specificities of FLIPQ-TV3.0 sensors.

(A) Saturation curves of FLIPQ-TV3.0 sensors with altered affinities. (B) Substrate specificities of FLIPQ-TV3.0_1.5 μ (black), 50 μ (hatched), 100 μ (white), 2 m (horizontal stripes), and 8 m (gray) sensors to Gln, Glu, Asn and Asp.

https://doi.org/10.1371/journal.pone.0140088.g001

In Table 1, the fifth and six row labels under the column heading Mutations in glnH are swapped. Please see the corrected Table 1 here.

In Fig 4A, the in-figure legends for R75MW220A and R75AY86A are swapped. Please view the correct Fig 4 here.

Reference

  1. 1. Gruenwald K, Holland JT, Stromberg V, Ahmad A, Watcharakichkorn D, Okumoto S (2012) Visualization of Glutamine Transporter Activities in Living Cells Using Genetically Encoded Glutamine Sensors. PLoS ONE 7(6): e38591. pmid:22723868