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Correction: The Crystal Structure and Small-Angle X-Ray Analysis of CsdL/TcdA Reveal a New tRNA Binding Motif in the MoeB/E1 Superfamily

  • Miguel López-Estepa,
  • Ana Ardá,
  • Martin Savko,
  • Adam Round,
  • William E. Shepard,
  • Marta Bruix,
  • Miquel Coll,
  • Francisco J. Fernández,
  • Jesús Jiménez-Barbero,
  • M. Cristina Vega
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There are multiple errors in Table 1. Please see the corrected Table 1 here.

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Table 1. Crystallographic data processing and refinement statistics.

https://doi.org/10.1371/journal.pone.0134070.t001

There is an error in the last sentence of the “TcdA crystallization, structure determination and refinement” subsection of the Materials and Methods. The correct sentence is: A data set for the AMP complex was collected to 1.80-Å resolution at the PROXIMA 2A beamline (Synchrotron SOLEIL, Paris, France). All the data sets were integrated with XDS [26] and scaled with Aimless [27] from the CCP4 suite of programs [28] (Table 1).

There are errors in the fifth and sixth sentences of the “Crystal structure of TcdA” subsection of the Results and Discussion. The correct sentences are: To shed light onto the structural basis for the tRNA binding and ct6A synthetic properties of TcdA-ATP, we determined the crystal structure of E. coli TcdA (Table 1 and Fig 2) loaded with ATP to 1.77 Å resolution (R/Rfree values of 0.141/0.183) (Fig 3A) and AMP to 1.80 Å resolution (R/Rfreevalues of 0.139/0.176) (Fig 3B). The asymmetric unit contained four TcdA chains arranged in two independent dimers, with a solvent content of 39%.

Reference

  1. 1. López-Estepa M, Ardá A, Savko M, Round A, Shepard WE, Bruix M, et al. (2015) The Crystal Structure and Small-Angle X-Ray Analysis of CsdL/TcdA Reveal a New tRNA Binding Motif in the MoeB/E1 Superfamily. PLoS ONE 10(4): e0118606. pmid:25897750