Can Newts Cope with the Heat? Disparate Thermoregulatory Strategies of Two Sympatric Species in Water

Monika Balogová; Lumír Gvoždík

doi:10.1371/journal.pone.0128155

Abstract

Many ectotherms effectively reduce their exposure to low or high environmental temperatures using behavioral thermoregulation. In terrestrial ectotherms, thermoregulatory strategies range from accurate thermoregulation to thermoconformity according to the costs and limits of thermoregulation, while in aquatic taxa the quantification of behavioral thermoregulation have received limited attention. We examined thermoregulation in two sympatric newt species, Ichthyosaura alpestris and Lissotriton vulgaris, exposed to elevated water temperatures under semi-natural conditions. According to a recent theory, we predicted that species for which elevated water temperatures pose a lower thermal quality habitat, would thermoregulate more effectively than species in thermally benign conditions. In the laboratory thermal gradient, L. vulgaris maintained higher body temperatures than I. alpestris. Semi-natural thermal conditions provided better thermal quality of habitat for L. vulgaris than for I. alpestris. Thermoregulatory indices indicated that I. alpestris actively thermoregulated its body temperature, whereas L. vulgaris remained passive to the thermal heterogeneity of aquatic environment. In the face of elevated water temperatures, sympatric newt species employed disparate thermoregulatory strategies according to the species-specific quality of the thermal habitat. Both strategies reduced newt exposure to suboptimal water temperatures with the same accuracy but with or without the costs of thermoregulation. The quantification of behavioral thermoregulation proves to be an important conceptual and methodological tool for thermal ecology studies not only in terrestrial but also in aquatic ectotherms.

Citation: Balogová M, Gvoždík L (2015) Can Newts Cope with the Heat? Disparate Thermoregulatory Strategies of Two Sympatric Species in Water. PLoS ONE 10(5): e0128155. https://doi.org/10.1371/journal.pone.0128155

Academic Editor: Carlos A. Navas, University of Sao Paulo, BRAZIL

Received: January 18, 2015; Accepted: April 22, 2015; Published: May 20, 2015

Copyright: © 2015 Balogová, Gvoždík. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Data Availability: All data files are available from the DRYAD database (doi:10.5061/dryad.tn32c).

Funding: Funding provided by: LG: Czech Science Foundation (15-07140S) and institutional support (RVO: 68081766); MB: European Operational Programme ‘KVARK-The quality of education and development of skills of PhD students and postdocs’ (26110230084). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

Introduction

Fast climate change poses a serious challenge to the current biota. The ability of organisms to withstand the impact of climate change primarily depends on their actual exposure to changed climatic conditions [1]. Many ectotherms are not passive to the variation of the thermal environment and reduce the exposure to environmental temperatures using behavioral thermoregulation [2]. Recent analysis confirmed the key role of this behavior in coping with climate change in terrestrial insects, amphibians, and squamate reptiles [3]. They also showed its limitation depending on the availability of cool refugia in ectotherm habitats, which suggests that lowland tropical and desert species are more vulnerable to a warming climate than temperate taxa [4–5]. However, the scarcity of information about the effectiveness of behavioral thermoregulation in aquatic organisms complicates understanding of the general trend in ectotherm exposure to climate change.

Although the physical characteristics of water, high heat capacity and fast thermal conductivity prevent small-bodied ectotherms from maintaining their T_b above or below temperatures of the surrounding medium, many aquatic organisms are capable of behavioral thermoregulation by moving across a thermally-stratified water column [6–7]. The ongoing rapid climate change increases the temperature in freshwater ecosystems providing, among other things, a new challenge for aquatic thermoregulators. Although the aquatic environment is more buffered against extreme temperatures than land, preferred body temperatures are generally lower in aquatic taxa than in their terrestrial counterparts [6, 8–10]. Accordingly, relatively small increases in temperature in shallow waters may markedly increase the proportion of available water temperatures above an ectotherm’s target thermoregulatory range. A recently published model [11] predicts an ecthotherm’s thermoregulation in a predominantly warm environment. The model assumes that the impact of suboptimally high body temperatures on organism performance is more severe than on the colder (ascending) part of the thermal performance curve [9]. If environmental temperatures frequently exceed an ectotherm’s target range, its thermoregulatory effectiveness should increase as the thermal quality of its habitat (proportion of operative temperatures outside the target range) decreases. Unfortunately, whether this prediction holds also for aquatic thermoregulators remains unknown.

In this study, we examined aquatic behavioral thermoregulation in two species of newts, Ichthyosaura alpestris and Lissotriton vulgaris, exposed to elevated environmental temperatures. Newts are suitable for this task for three reasons. First, adult newts use thermoregulatory behavior in water [12]. Second, thermal conditions in newt aquatic habitats, i.e. shallow pools and ponds, provide various opportunities for behavioral thermoregulation in the field [13]. Finally, although both species sometimes co-occur in water bodies, the Czech populations of I. alpestris largely occupy forested areas at altitudes above 400 m, while L. vulgaris commonly occurs in open landscapes well below and above this altitudinal level [14–16]. Hence, from a thermal ecology view, the variation in habitat use suggests diverse thermal requirements or thermoregulatory effectiveness and accordingly, the exposure to climate change in both newt species.

We predicted that elevated water temperatures would induce a higher thermoregulatory effort in newt species for which the thermal conditions pose a lower thermal quality environment than for the species with thermal requirements largely matching available water temperatures. To test this prediction, we subjected both species to the same thermal conditions, which were slightly warmer for L. vulgaris and substantially warmer for I. alpestris than water temperatures in their native habitats (Fig 1). Local climate warming trend [17] indicate that populations of the later species would be exposed to these temperatures in the near future.

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Fig 1. Water temperatures experienced by newts, Ichthyosaura alpestris and Lissotriton vulgaris, in native and semi-natural aquatic habitats between 6^th and 19^th June 2014.

(a) Water temperatures at the maximum depth, (b) temperatures on water surface. Values (hourly means±extremes) are shifted horizontally to reduce overlap.

https://doi.org/10.1371/journal.pone.0128155.g001

Materials and Methods

Study Species and Maintenance

Lissotriton vulgaris and I. alpestris are small to medium-sized newts (total length up to 90 and 120 mm, respectively), which are widely distributed across Europe [14–15]. Both species share a similar lifestyle. Newts usually spend part of the year in water (April–June) and the rest of the year on land. During their aquatic phase, they occupy a variety of still waters, such as pools, ponds, and lakes. Although newts are largely crepuscular and nocturnal, they are also active during daytime. Their aquatic diet consists of diverse invertebrates, ranging from planktonic crustaceans to oligochaetes and chironomid larvae. Preferred body temperatures provide the best compromise for various thermal reaction norms and performance curves of fitness-related traits in newts [18–19].

We captured adult newts (I. alpestris: n = 30 [1:1 sex ratio], snout-vent length [SVL] = 45.7± 3.7 mm [mean±SD]; L. vulgaris: n = 30 [1:1 sex ratio], SVL = 38.9± 2.8 mm) from two water bodies, located 4 km apart, near Jihlava, Czech Republic. The pool occupied by I. alpestris was located in a forest at 600 m altitude (49°23'18"N, 15°30'48"E), whereas the pool with L. vulgaris was situated in open habitat at an altitude of 560 m (49°22'56"N, 15°33'10"E). Both populations were chosen according to their high abundance to provide sufficient sample size and a minimum distance from each other.

Newts (one male and one female) were placed in plastic aquaria filled with 18 l of non-chlorinated well water. Each aquarium was equipped with aquatic weeds (Egeria densa) and a piece of Styrofoam allowing newts to leave water. Aquaria were placed in a room with a natural light:dark cycle and diel thermal fluctuations 12–22°C. Temperatures were selected according to the most frequent temperatures in their natural habitat [17]. Newts were fed with live Chironomus larvae, Tubifex worms, and Eisenia eartworms once or twice per week. Water (50% of total volume) was changed on weekly basis.

Preferred Body Temperatures

Preferred body temperatures, i.e., body temperatures maintained by an ectotherm under the absence of thermoregulatory constraints, were measured in a stainless steel tank (240×60×60 cm high). The tank was divided into three longitudinal lanes. Each lane consists of 12 partially separated compartments (20×20 cm) with different water temperatures. The bottom of each compartment was equipped with Peltier modules that were connected together with a heat recuperation system to a programmable control unit, which allowed for the creation of a horizontal thermal gradient in water. We used thermal gradients between 8 and 30°C in 2±0.5°C steps. The tank was filled with non-chlorinated well water up to 3 cm. Mild aeration promoted thermal homogenization of water in each compartment. The tank was placed in a room at 18±0.5°C. Series of fluorescent daylight tubes above the tanks provided light intensity of 300 lx on the water surface.

Since reproduction and satiation level influence T_p in newts [20–21], we used non-reproductive and fully fed individuals for its measurements. In the field, most newts contain food in their stomachs [22], and so we considered the T_p of fully fed individuals more ecologically realistic than the T_p of hungry newts. Three randomly chosen newts were placed individually into the tank lane 15 hrs before the beginning of trials. Their behavior was recorded using a digital camera (12 fps; MTV-63S80H-A-ICR-R, Mintron, Taipei, Taiwan) connected to a PC surveillance system (V-Guard RT4, Chateau, Taipei, Taiwan) between 10:00 and 17:00. Water was changed after each trial. Six thermistor probes connected to dataloggers (resolution 0.1°C; HOBO, Onset Computer, Bourne, MA, USA) recorded the minimum and maximum temperatures in each lane to monitor the stability of the thermal gradient during trials.

Later, video recordings were checked and newt horizontal positions in the thermal gradient (compartment) were recorded at 10 min intervals. Since the T_b of small ectotherms quickly equilibrate with the temperature of the surrounding medium [23], we estimated newt T_b indirectly from the known water temperatures in a given compartment. Data from newts that were inactive or left the water for a prolonged amount of time (>90%) were discarded from further analyses. From individual T_b distribution (n = 216), we calculated the lower and upper T_p boundaries as the 10th and 90th percentiles, respectively. We assumed that these values delimit the target range of T_b that newts aim to achieve by behavioral thermoregulation.

Operative and Body Temperatures

To compare behavioral thermoregulation of both species under elevated temperatures and without the confounding influence of biotic factors, we performed thermoregulatory trials in tanks under semi-natural conditions. We used 14 fiberglass tanks (90×63×47 cm high) that were half-buried in soil in two rows in an east-west direction. The bottom of each tank was profiled (15 cm flat, 60 cm inclined, 15 cm flat) allowing bottom-walking newts to move across the water columns. Tanks were filled with non-chlorinated well water up to 45 cm. Each 5 cm of water column was marked on the bottom to record the newt’s vertical position. Two marginal and one middle tank contained series of nine waterproofed dataloggers (resolution 0.5°C; DS1921G-F5, Maxim Integrated Products, Sunnyvale, CA, USA) that recorded water temperatures at each 5 cm of the water column at 30 min intervals. The difference between cages was always ≤1°C, which indicates that the dataloggers sufficiently mapped thermal conditions in the experimental containers. Since the T_b of small ectotherms quickly equilibrate with water temperatures, the physical properties of newt bodies have a minor influence on their equilibrium T_b. Accordingly, we considered water temperatures as the reliable T_e estimates, i.e., equilibrium temperatures of an inanimate object with the same physical properties as the studied ectotherm [24], in both species. The vertically stratified T_e profile represents a “null-distribution” of T_bs that non-thermoregulating newts would achieve.

To evaluate newt thermoregulatory behavior, we placed randomly chosen newts individually into experimental tanks two hours before the beginning of each trial. One person (MB) walked slowly along tanks and recorded newt vertical positions (resolution 5 cm) at hourly intervals between 10:00 and 17:00. Our previous observations confirmed that the presence of an observer had no influence on newt behavior. Similarly to T_p, we estimated newt T_b indirectly from their vertical position on the profiled bottom with known water temperature. All experiments were performed between 3^rd and 19^th June 2014.

Thermoregulatory Indices

Using information from the T_p range, T_e and T_b, three thermoregulatory indices were calculated [24, 25]. 1. Thermal quality of habitat: The deviation of mean T_e from the T_p range. If T_e falls within the range, d_e equals zero. The low index value indicates high thermal quality of habitat for focal species and vice versa. Since T_e varied substantially in time, the index was calculated for each individual at hourly intervals. 2. Accuracy of thermoregulation (d_b): Calculated in the same way as d_e with T_b instead of T_e. 3. Effectiveness of thermoregulation (E): The index was originally calculated as E = d_b/d_e. Since the ratio may provide poor quantification of thermoregulation, we used an alternative calculation as E = d_e–d_b [25]. Values around zero indicate thermoconformity, positive values active thermoregulation, whereas negative values suggest the active avoidance of target temperatures.

Statistical Analyses

Prior to model testing, we performed exploratory analyses involving the detection of possible outliers, non-normality, heterogeneity, non-independence, and the covariation of explanatory variables. Since we obtained repeated measurements of T_e and T_b during trials, their variation was analyzed using a general linear mixed model for short time series [26]. The full model consists of three fixed factors and their interactions, species, time of day, and date of measurement. The model variance structure was improved using date of measurement (T_e analysis) or species (T_b analysis) as the variance covariate. Individual identity (intercept) and individual identity in time (intercept and slope) were added as random factors. The optimal model structure was selected using the likelihood approach, i.e. we compared the model maximum likelihood for fixed factors and the restricted maximum likelihood for random factors. Since T_p boundaries and thermoregulatory indices did not meet some of the assumptions (outliers, non-normality) mentioned above, we applied a robust randomization (permutation) approach for statistical inference. The full model contained the same factors as above. The optimal model structure was selected according to factor variance components, i.e. factors that explained no variance in the examined trait was dropped from the model. In all randomization tests, we used 9999 permutations to calculate P values. Confidence intervals for sample means were obtained using a non-parametric bootstrap procedure (9999 replications). Statistical analyses were performed using ‘perm’ [27], ‘nlme’ [28] and ‘boot’ [29] libraries in R [30] and ‘PERMANOVA’ package for PRIMER (version 6; Primer E, Lutton, UK).

Results

Preferred body temperatures were measured in 15 individuals of each species. Three I. alpestris and one L. vulgaris remained motionless during the whole trial, and thus their data were removed from further analyses. Since factors ‘sex’ and ‘sex×species’ explained only minor variations in the T_p characteristic (mean, lower boundary, and upper boundary), they were dropped from the minimum adequate model. Ichthyosaura alpestris maintained a lower mean T_p and upper T_p boundary than L. vulgaris (mean: I. alpestris: 17.9°C, 95% CI [16.7, 18.7]; L. vulgaris: 21.7°C, 95% CI [20.5, 22.5]; pseudo-F_1,30 = 12.42, P < 0.001; upper boundary: pseudo-F_1,30 = 17.28, P < 0.001; Fig 2). Between-species variation in lower T_p boundary was statistically non-significant (pseudo-F_1,30 = 3.49, P = 0.07; Fig 2).

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Fig 2. Lower and upper boundaries (mean ± 95%CI) of preferred body temperatures in two newt species, Ichthyosaura alpestris and Lissotriton vulgaris.

Boundaries were calculated from 10^th and 90^th percentiles of individual distributions. Datapoints are jittered horizontally to reduce overlap.

https://doi.org/10.1371/journal.pone.0128155.g002

Operative temperatures increased during daytime in a curvilinear fashion (Fig 3a and 3b; Table 1). Both species experienced a similar T_e (χ² = 0.10, P = 0.75). The daily course of T_b showed the same trend as T_e (Fig 3c and 3d; Table 1). Mean T_b were consistently lower in I. alpestris than in L. vulgaris (χ² = 13.13, P < 0.001; Table 2).

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Fig 3. Operative and body temperatures as a function of the time of day in adult Ichthyosaura alpestris and Lissotriton vulgaris in semi-natural settings during June.

Datapoints (mean operative temperatures across the water columns and individual body temperatures) are jittered horizontally to reduce overlap. Data are fitted with a general linear mixed model (±95% CI). See Table 2 for model parameters. Horizontal dashed lines denote the lower and upper T_p boundaries.

https://doi.org/10.1371/journal.pone.0128155.g003

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Table 1. Fixed factor parameters of the minimum adequate models explaining the effect of daytime and species on operative and body temperatures of adult newts, Ichthyosaura alpestris and Lissotriton vulgaris, during June.

https://doi.org/10.1371/journal.pone.0128155.t001

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Table 2. Summary statistics of thermoregulatory characteristics of adult newts, Ichthyosaura alpestris and Lissotriton vulgaris.

https://doi.org/10.1371/journal.pone.0128155.t002

Given the variation in d_e, semi-natural conditions provided lower thermal quality for I. alpestris than for L. vulgaris (pseudo-F_1,64 = 24.22, P < 0.001; Table 2). Newts maintained their T_b within the T_p range with the same accuracy in both species (pseudo-F_1,61 = 0.01, P = 0.92). Accordingly, the E index markedly differed between species (pseudo-F_{1, 64} = 31.17, P < 0.001). The index values indicate active thermoregulation in I. alpestris and thermoconformity in L. vulgaris (Table 2). Thermoregulatory effectiveness was consistent during the day in both species (Hour: pseudo-F_6,267 = 1.29, P = 0.27; Hour × Species: pseudo-F_6,267 = 1.51, P = 0.17). Newt individual identity explained 23% of total variance in E (pseudo-F_54,267 = 2.81, P < 0.001).

Discussion

Unlike the long research tradition in terrestrial ectotherms, the quantification of behavioral thermoregulation in aquatic taxa has received surprisingly little attention. We demonstrated that two sympatric newt species adopted disparate thermoregulatory strategies, active thermoregulation and thermoconformity, to cope with elevated temperatures in water. The species exposed to the lower quality thermal habitat thermoregulated with a higher effort than species in thermally more benign conditions, which corroborates the prediction of the current theory [11]. Thermoconforming L. vulgaris maintained a higher T_p and within a wider range than the thermoregulating I. alpestris, which suggests that the variation in thermoregulatory strategies largely reflects the variation in their T_p ranges. Both strategies allowed newts to keep their T_b within the target range with the same accuracy but with or without the costs of thermoregulation.

Although the costs of thermoregulation were not quantified in this study, the available information provides at least a qualitative view on this issue. Our results showed that the behavioral thermoregulation forced I. alpestris to cooler and deeper waters than L. vulgaris. Accordingly, the reduced activity space may restrict the opportunity to find food and increase the time and energy costs of aerial breathing because of prolonged traveling distances to the water surface [18]. Contrary to thermoregulatory costs in terrestrial taxa, which include time and energy spent thermoregulating, predation risk, or missed opportunity [31], the presumed costs in thermoregulating newts are indirect, i.e., restricted foraging space and the increased time and energy demands of aerial breathing. Further research will show how the indirect costs of thermoregulation affect the ultimate currency, fitness or its components, in newts.

Why did sympatric species achieve the same accuracy of thermoregulation by adopting disparate thermoregulatory strategies? As mentioned above, these strategies largely reflect between species variation in T_p ranges. The exact source of T_p variation is unknown. Among species, T_p may be affected by their independent phylogenetic history, though the evolutionary rates (the change in an evolutionary lineage over time) of T_p are rather low in newts [32]. Within-species, thermally-induced developmental plasticity [33] may also contribute to this result. This suggests that newt thermoregulatory strategies in an aquatic environment is primarily determined by factors limiting the variation in target temperatures, which newts aim to achieve.

The E values indicate that under elevated water temperatures I. alpestris was able to maintain T_b that were on average 1.3°C below the mean T_e. In comparison with E values in terrestrial ectotherms [34], the value is fairly low suggesting that newts thermoregulated with much lower effectiveness in water. Unfortunately, we are not aware of any study reporting estimates of thermoregulatory effectiveness in other aquatic taxa. Hence, further research will show whether newt thermoregulation is less effective than in terrestrial taxa because of the physical characteristics of water, phylogenetic distance, or other factors.

While both species effectively coped with elevated water temperatures, further warming would affect thermoregulating I. alpestris more than thermoconforming L. vulgaris, not only because of the costs of thermoregulation, but also of prolonged time, when thermal conditions prevent behavioral thermoregulation. The negative effect of thermal constraints could be avoided in three ways. First, newts could simply prefer deeper water bodies, which provide sufficiently cold refugees. However, this depends on the availability of suitable water bodies, which should be not only deep enough, but provide other suitable biotic and abiotic conditions [16]. Second, newts could leave the aquatic habitat before it became too warm. However, newts from southern populations respond to hot and dry weather by staying in water year-round rather than by reducing their aquatic period [35]. Finally, newts could shift their T_p range. Given their long generation time and evolutionary rates of T_p, the only viable alternative is a plastic response (see above). However, whether the plastic shift induced during embryonic development [33] persists for the rest of life is unknown. In addition, the magnitude of a seasonal plastic response is relatively low [13] and information about its costs and limits [36] are lacking. Taking into account also the negative influence of elevated water temperatures on larvae [37], I. alpestris would likely respond to climate warming by restricting its occurrence to cooler habitats [3, 5].

Our study was performed in seminatural settings, which eliminated many unwanted factors at the expense of lower ecological realism. Alpine newts were intentionally exposed to much higher water temperatures than in their native habitat, and thus it can be argued that our results were affected by ecologically unrealistic thermal conditions. However, the maximum water temperatures were not higher than temperatures that newts experienced in the sun-exposed pool located close to the population studied [17]. Hence, we considered the thermal profile used a reasonable estimate of conditions that newts would face as a result of deforestation, heat waves [38], or local climate change in the near future.

Conclusions

Our study provides a rare attempt to quantify thermoregulatory strategies in aquatic ectotherms. Adopting the same approach as terrestrial ecologists [24], our results revealed two attributes that aquatic temperate newts share surprisingly not with temperate, but with tropical terrestrial ectotherms. First, while temperate ectotherms thermoregulate effectively despite thermally challenging conditions [25, 39–40], tropical species frequently become thermoconformers in thermoregulatory costly habitats [24, 41]. Hence, the variation in thermoregulatory strategies of aquatic newts is more similar to tropical rather than temperate species on land. Second, the major task for temperate thermoregulators is to heat the body above the average environmental temperature, whereas tropical thermoregulators have to solve the opposite issue, i.e. how to cool their T_b [2, 11]. We showed that under elevated water temperatures, one newt species was faced with the same task as tropical ectotherms. Hence, our study challenged the tropical-temperate patterns in behavioral thermoregulation. This proves that the quantification of thermoregulatory strategies in at least some aquatic ectotherms provides a similarly important tool for understanding their thermal ecology and responses to climate change as in terrestrial taxa.

Acknowledgments

We thank U. Sinch and an anonymous reviewer for their comments on the previous version of this paper; P. Kristín, V. Kršáková, and R. Smolinský for their help with capturing newts and preparing experiments.

Author Contributions

Conceived and designed the experiments: LG. Performed the experiments: MB. Analyzed the data: LG. Contributed reagents/materials/analysis tools: LG. Wrote the paper: LG MB.

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Can Newts Cope with the Heat? Disparate Thermoregulatory Strategies of Two Sympatric Species in Water

Can Newts Cope with the Heat? Disparate Thermoregulatory Strategies of Two Sympatric Species in Water

Correction

Figures

Abstract

Introduction

Materials and Methods

Study Species and Maintenance

Preferred Body Temperatures

Operative and Body Temperatures

Thermoregulatory Indices

Statistical Analyses

Results

Discussion

Conclusions

Acknowledgments

Author Contributions

References