Cooling Reduces cAMP-Stimulated Exocytosis and Adiponectin Secretion at a Ca2+-Dependent Step in 3T3-L1 Adipocytes

Mickaël F. El Hachmane; Ali M. Komai; Charlotta S. Olofsson

doi:10.1371/journal.pone.0119530

Abstract

We investigated the effects of temperature on white adipocyte exocytosis (measured as increase in membrane capacitance) and short-term adiponectin secretion with the aim to elucidate mechanisms important in regulation of white adipocyte stimulus-secretion coupling. Exocytosis stimulated by cAMP (included in the pipette solution together with 3 mM ATP) in the absence of Ca²⁺ (10 mM intracellular EGTA) was equal at all investigated temperatures (23°C, 27°C, 32°C and 37°C). However, the augmentation of exocytosis induced by an elevation of the free cytosolic [Ca²⁺] to ~1.5 μM (9 mM Ca²⁺ + 10 mM EGTA) was potent at 32°C or 37°C but less distinct at 27°C and abolished at 23°C. Adiponectin secretion stimulated by 30 min incubations with the membrane permeable cAMP analogue 8-Br-cAMP (1 mM) or a combination of 10 μM forskolin and 200 μM IBMX was unaffected by a reduction of temperature from 32°C to 23°C. At 32°C, cAMP-stimulated secretion was 2-fold amplified by inclusion of the Ca²⁺ ionophore ionomycin (1μM), an effect that was not observed at 23°C. We suggest that cooling affects adipocyte exocytosis/adiponectin secretion at a Ca²⁺-dependent step, likely involving ATP-dependent processes, important for augmentation of cAMP-stimulated adiponectin release.

Citation: El Hachmane MF, Komai AM, Olofsson CS (2015) Cooling Reduces cAMP-Stimulated Exocytosis and Adiponectin Secretion at a Ca²⁺-Dependent Step in 3T3-L1 Adipocytes. PLoS ONE 10(3): e0119530. https://doi.org/10.1371/journal.pone.0119530

Academic Editor: Makoto Kanzaki, Tohoku University, JAPAN

Received: October 10, 2014; Accepted: January 30, 2015; Published: March 20, 2015

Copyright: © 2015 El Hachmane et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Data Availability: All relevant data are within the paper.

Funding: Funding provided by Jeanssons Stiftelse, http://www.jeanssonsstiftelser.se/, Åke Wibergs Stiftelse, http://ake-wiberg.se/, Magnus Bergvalls Stiftelse, http://www.magnbergvallsstiftelse.nu/, Diabetesfonden (DIA-2011-073, DIA2012-050 and DIA2013-070), http://diabetesfonden.se/, Novonordiskfonden, http://www.novonordiskfonden.dk/en, Knut och Alice Wallenbergs Stiftelse (Dnr KAW2012.0263), https://www.wallenberg.com/kaw/, IngaBritt och Arne Lundbergs Stiftelse (Grant ID: 401), http://www.lundbergsstiftelsen.se/ and Swedish Medical Research Council (Grant IDs: 521-2012-2994 and 522-2010-2656), http://vr.se/. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

Introduction

White adipose tissue is an endocrine organ that secretes a variety of hormone-like protein factors, commonly referred to as adipokines. Adiponectin is a protein hormone secreted exclusively from white adipose cells and involved in modulation of glucose and lipid metabolism [1].

Regulated exocytosis has been investigated in several neuroendocrine cell types and is known to involve Ca²⁺, ATP and temperature-dependent steps [2]. Adiponectin has been suggested to be secreted via regulated exocytosis of a pre-stored pool of secretory vesicles [3,4] but very little is known about stimulus-secretion coupling in the white adipocyte. Our recent work has shown that adipocyte exocytosis/adiponectin secretion is stimulated via PKA-independent cAMP-stimulation in a Ca²⁺-independent manner and is amply augmented by a combination of Ca²⁺ and ATP [5]. Thus, unlike archetypal endocrine cell types, Ca²⁺ amplifies but does not trigger exocytosis in adipocytes.

Characterisation of the steps of stimulus-secretion coupling, such as vesicle replenishment and fusion [2], is crucial in order to understand exocytosis. Several studies have shown that Ca²⁺-dependent exocytosis exhibits temperature-dependent components in both neuronal [6] and endocrine [7–9] cell types. Temperature-dependent exocytotic mechanisms were first described in catecholamine secreting chromaffin cells and the temperature-sensitive step was suggested to represent a rate-limiting late exocytotic component [7]. A similar temperature-dependent enhancement of exocytosis was reported in pancreatic beta-cells [8] and melanotrophs [9] and was suggested to reflect an increased supply of vesicles ready for release. Moreover, insulin-stimulated translocation of glucose transporter 4 (Glut4) containing vesicles in white adipocytes is inhibited by temperature reduction [10].

Here we have investigated the temperature dependence of exocytosis and short-term adiponectin release in 3T3-L1 adipocytes, a widely accepted and commonly used model of primary adipocytes. The aim of the study was to elucidate mechanisms important in regulation of white adipocyte stimulus-secretion coupling. Electrophysiological recordings of exocytosis (increase in membrane capacitance) were compared to measurements of secreted adiponectin under similar conditions. We report that a temperature drop is without effect on cAMP-stimulated exocytosis/adiponectin secretion but that cooling completely abolishes the augmentation of exocytosis/secretion induced by Ca²⁺.

Material and Methods

Cell culture and differentiation

3T3-L1 adipocytes (mouse origin) were maintained and differentiated according to established protocols [11] and as previously described [5]. Experimental studies were carried out in differentiated 3T3-L1 adipocytes between day 7 and 9 from start of differentiation. The level of differentiation was visually determined (degree of lipid filling) and only cells regarded to be fully mature (as determined by the intracellular presence of large lipid droplets) were chosen for experiments (Fig. 1A). In adiponectin secretion studies, only wells with a visually assessed differentiation level of ≥95% were used.

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Fig 1. Undifferentiated and mature 3T3-L1 adipocytes as well as demonstration of analysis performance.

A Example of 3T3-L1 adipocytes in the fibroblast-like state before differentiation (left) as well as when differentiated into mature adipocytes (right). B Representative capacitance recording with ΔC_m (delta membrane capacitance) plotted against time showing how analyses were carried out. ΔC/Δt was measured at the time intervals indicated by the dotted lines by fitting straight functions to the data points (red lines superimposed on the black capacitance trace). Scale bar = 50μm.

https://doi.org/10.1371/journal.pone.0119530.g001

Electrophysiology

Exocytosis was measured as increase in membrane capacitance [12] using the whole-cell configuration of the patch-clamp technique and an EPC-9 patch-clamp amplifier (HEKA Electronics, Lambrecht/Pfalz, Germany and PatchMaster software). Patch pipettes were pulled from borosilicate glass capillaries and coated with Sylgard (Dow Corning Corporation). Pipettes were heat-polished prior to use. The pipette resistance was typically 3-5 MΩ when filled with pipette-filling solution and access resistance after establishment of the whole-cell mode was ≤10 MΩ. Cells were voltage-clamped at -70 mV during the recordings.

3T3-L1 adipocytes, grown in Petri culture dishes (Nunc, Denmark), were continuously superfused with an extracellular solution (EC) containing (in mM) 140 NaCl, 3.6 KCl, 2 NaHCO₃, 0.5 NaH₂PO₄, 0.5 MgSO₄, 5 HEPES (pH 7.4 with NaOH), 2.6 CaCl₂ and 5 mM glucose (EC). The pipette filling solutions consisted of (in mM) 125 potassium glutamate, 10 KCl, 10 NaCl, 1 MgCl₂, 10 EGTA, 3 Mg-ATP, 0.1 cAMP and 5 HEPES (pH 7.15 with KOH). The solution was in some experimental series supplemented with 9 mM CaCl₂ (yielding a free Ca²⁺ concentration of ~1.5 μM calculated using http://www.stanford.edu/~cpatton/maxc.html.). Experiments were conducted at 23°C, 27°C, 32°C or 37°C as specified. The temperature in the cell culture dish was regulated by an in-house feed-back controller.

Measurements of intracellular Ca²⁺ concentrations

The intracellular Ca²⁺ concentration ([Ca²⁺]_i) was recorded with dual-wavelength ratio imaging as previously described [13]. Briefly, cells were cultured in glass bottom dishes (MatTek Corporation, USA) and loaded with the membrane-permeable acetoxymethyl ester of fura-2 (fura-2 AM; Life Technologies) during 1 hour in EC at room temperature with gentle shaking. A Lambda DG-4 illumination system was employed (Sutter Instrument Company, USA) and images were captured using a QuantEM 512SC CCD camera (Photometrics, USA) and MetaFluor software (Meta imaging series 7.5, Molecular Devices, USA). Cells were alternately excited at 340 and 380 nm and emitted light was collected at 510 nm. Ratios were converted into [Ca²⁺]_i using equation (5) of [14] and a K_d of 224 nM. Values of maximum (R_max) and minimum (R_min) ratios as well as S_f2/S_b2 were determined using solutions with a high Ca²⁺ concentration (12 mM CaCl₂ + 10 mM EGTA) or lacking Ca²⁺ (10 mM EGTA alone). Measurements were carried out at 23°C or 32°C as indicated.

Studies of secreted adiponectin

3T3-L1 adipocytes were grown and differentiated in 6- or 12-well plates (Sarstedt or Nunc, respectively) and secretion studies were carried out as previously described [5]. Briefly, cells were pre-incubated for 30 minutes in EC lacking glucose and in the presence or absence of BAPTA-AM (Life Technologies) as specified. EC, supplemented with test substances as indicated, was added and cells were incubated on gentle shaking for 30 min at 23°C or 32°C in parallel. At the end of incubation the EC was removed, centrifuged, aliquoted and samples were stored at -80°C awaiting analysis. Secreted adiponectin was measured using a mouse Adiponectin ELISA kit (R&D Systems Europe, UK) and expressed relative to total cellular protein (measured with Pierce BCA protein, Thermo Scientific, USA).

Data analysis

The rate of capacitance increase (ΔC/Δt) was measured by applying a linear fit to the curve at specified time points as shown in Fig. 1B. The statistical significance of variance between two means was calculated with one-way ANOVA and Student’s t-test, paired or unpaired as appropriate using OriginPro (OriginLab Corporation, USA). All data are presented as mean values ± S.E.M. for the designated number of experiments.

Results

The effect of temperature on 3T3-L1 adipocyte exocytosis

In order to investigate the temperature dependence of exocytosis in adipocytes, we compared the exocytotic rate at 23°C, 27°C, 32°C and 37°C in cells dialysed with a Ca²⁺-free pipette solution containing 0.1 mM cAMP (10 mM EGTA and 3 mM ATP included) known to trigger adipocyte exocytosis [5]. The rate of exocytosis (ΔC/Δt) was measured at the indicated time intervals as shown in Fig. 1B. Exocytosis triggered by intracellular cAMP proceeded at a rate comparable to that reported previously [5] and was not affected by varying the bath temperature (Fig. 2A and B). We next investigated effects of temperature on exocytosis in cells infused with the same solution supplemented with 9 mM CaCl₂ (free [Ca²⁺] ~1.5 μM). In agreement with what we have previously reported [5], exocytosis was potentiated by inclusion of Ca²⁺ and the rate at 32°C was ≥2-fold amplified compared to 0 Ca²⁺ at all investigated time points (Fig. 2C and D). As shown in Fig. 2C and D, exocytosis was reduced by cooling and the rate at 2.5 min (ΔC/Δt_{2.5 min}) after establishment of the whole-cell configuration amounted to only ~30% of that recorded at 32°C or 37°C.

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Fig 2. cAMP-stimulated exocytosis is unaffected by cooling while Ca²⁺ potentiation is diminished by temperature reduction.

A Representative traces of membrane capacitance increases (ΔC_m) evoked by infusion of 0.1 mM cAMP in the absence of intracellular Ca²⁺. B Average ΔC/Δt at indicated time points using the Ca²⁺ free intracellular solution. Note that ΔC/Δt is equal at all times, regardless of the temperature in the cell dish. C, D As in (A) and (B) respectively, but with 1.5 μM free Ca²⁺ added to the pipette solution. Observe the Ca²⁺-dependent augmentation of exocytosis as well as the temperature-dependence in the presence of Ca²⁺. n = 7–9; ***P<0.001; **P<0.01; *P<0.05 vs. 23°C. (P = 0.07 for 27°C vs. 23°C at t = 5 min. P = 0.1 at t = 10 for 37°C vs. 23°C)

https://doi.org/10.1371/journal.pone.0119530.g002

Q10 values of exocytosis

The temperature coefficient (Q₁₀) can be calculated for the rate of a process as a consequence of raising the temperature by 10°C. While diffusion of ions through their respective channels are modestly affected by a lowering of temperature, biological processes involving enzymatic reactions or protein conformational changes usually display greater temperature dependence (Q₁₀ value ≥2) [15]. We estimated Q₁₀ values of 1.4 and 3.3 in the absence and presence of 1.5 μM free Ca²⁺ respectively. To further explore the temperature-dependence of adipocyte exocytosis, we constructed Arrhenius plots of exocytotic rates at the temperatures investigated (Fig. 3A and B). The energy of activation (E_A; slope of fitted line) amounted to 5.7 kJ mol^-1 in the absence of Ca²⁺ and 53 kJ mol^-1 in the presence of Ca²⁺. The Q₁₀ and E_A values indicate that exocytosis in the absence of Ca²⁺ is independent of temperature and requires little energy, whereas Ca²⁺-dependent exocytosis is a much more temperature-dependent and energy demanding process.

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Fig 3. Arrhenius plots presenting the effect of Ca²⁺ on the rate of cAMP-triggered exocytosis.

Absolute values of ΔC/Δt at t = 2 min plotted against the inverse of temperature (T) in the absence (A) and presence (B) of cytosolic Ca²⁺. The curve represents a linear least-squares fit to the equation lnk = lnA—E_A/RT. E_A is the energy of activation, k is the change in ΔC/Δt upon a temperature change, A is the pre-exponential factor. R represents the gas constant and T the absolute temperature as usual. The negative slope of the curve gives activation energies of 5.7 kJ mol^-1 (A) and 53 kJ mol^-1 (B).

https://doi.org/10.1371/journal.pone.0119530.g003

Effects of cooling on 3T3-L1 adipocyte adiponectin secretion

3T3-L1 adipocytes secrete substantial quantities of adiponectin [3,16] and we have previously documented a strong correlation between membrane capacitance increases and secretion of this adipokine [5]. In order to compare effects of temperature on exocytosis and adiponectin release, 3T3-L1 adipocytes were incubated in the presence of the membrane permeable cAMP agonist 8-Br-cAMP alone or in combination with the Ca²⁺-ionophore ionomycin (to maximally elevate [Ca²⁺]_i). Incubations were carried out for 30 minutes at 23°C or 32°C. Effects of ionomycin and 8-Br-cAMP on intracellular Ca²⁺ levels were validated by use of Ca²⁺ imaging. As shown in Fig. 4A, external application of ionomycin (1 μM at 23°C or 32°C) elevated [Ca²⁺]_i resulting in a peak at t = ~3 min, after which [Ca²⁺]_i declined towards baseline before stabilising at a plateau at 10 min (Fig. 4B). The magnitudes of the responses were comparable although the peak value was slightly (20 nM) but significantly higher at 32°C (P<0.001). However, the measured minute difference is likely of negligible physiological importance. An ionomycin-induced peak [Ca²⁺]_i of ~200 nM may appear too small to affect exocytosis but it has to be kept in mind that the measurements show the average [Ca²⁺]_i in the cytoplasm and may be several-fold higher at the release sites [17]. Inclusion of 8-Br-cAMP (1 mM) was without effect on [Ca²⁺]_i (Fig. 4C; [Ca²⁺]_i averaged 108±2 nM before and 119±3 nM after agonist application).

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Fig 4. Ionomycin, but not 8-Br-cAMP, elevates adipocyte [Ca²⁺]_i.

Example traces of [Ca²⁺]_i responses upon extracellular application of 1 μM ionomycin (A) or 1 mM 8-Br-cAMP (C). B Average responses to ionomycin at indicated time points between 0 and 15 min. Ionomycin or 8-Br-cAMP was added extracellularly to the dish of cells and remained present throughout the recording as indicated. Note that the peak response to ionomycin shown in (B) was slightly shifted at 23°C (peak at 3.6 min; 5 separate experiments and 122 cells) compared to 32°C (peak at 3.1 min; 4 experiments and 107 cells). The trace in (C) is representative for 101 analysed cells in 4 separate experiments.

https://doi.org/10.1371/journal.pone.0119530.g004

As can be seen in Fig. 5A, 8-Br-cAMP stimulated adiponectin secretion ~1.5-fold over control regardless of the temperature. Ionomycin was unable to augment release further at 23°C, whilst robustly potentiating secretion at 32°C. We further investigated the effect of temperature on adiponectin secretion stimulated by 10 μM forskolin in combination with 200 μM IBMX (forsk/IBMX). We have previously shown that forsk/IBMX elevates 3T3-L1 intracellular cAMP-levels >6-fold [5]. As shown in Fig. 5B, adiponectin secretion was stimulated >3-fold under those conditions, equally at 23°C and 32°C. Inclusion of ionomycin (1 μM) potentiated forsk/IBMX-stimulated secretion ~2-fold at 32°C but was without effect on release stimulated at 23°C. Our secretion data confirm the existence of a temperature-sensitive Ca²⁺-dependent step involved in 3T3-L1 adipocyte stimulus-secretion coupling and further underscore the strong association between 3T3-L1 adipocyte membrane capacitance increases and release of adiponectin.

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Fig 5. Ca²⁺-augmented adiponectin secretion is abolished by cooling while secretion stimulated by cAMP alone is unaffected.

A Adiponectin secretion as fold-increase compared to control stimulated by 8-Br-cAMP (1 mM) alone or in combination with ionomycin (1 μM) during 30 min incubations at 23°C (blue) or 32°C (red). B As in (A) but using forskolin (10 μM) and IBMX (200 μM; forsk/IBMX) as a stimulator. Data are mean values ± S.E.M. of 11 experiments at each temperature in (A) and 7 (23°C) and 8 (32°C) in (B). *P<0.05; **P<0.01; **P<0.001 vs. control at corresponding temperature. †P<0.01 vs. 8-Br-cAMP or forsk/IBMX alone at 32°C; ǂ P<0.05 vs. 8-Br-cAMP + ionomycin or forsk/IBMX + ionomycin at room temperature. The data in (B) at 32°C are the same as in Fig. 7B in [5].

https://doi.org/10.1371/journal.pone.0119530.g005

Discussion

In this study we have combined electrophysiological recordings of exocytosis with biochemical measurements of adiponectin release in order to elucidate mechanisms involved in regulation of white adipocyte stimulus-secretion coupling. Exocytosis is in archetypal neuroendocrine cells temperature sensitive [6–8]. Our study shows that exocytosis/adiponectin secretion in white adipocytes, in addition to temperature-independent processes, comprises temperature-dependent components involving Ca²⁺. We have recently shown that an elevation of cytosolic Ca²⁺ is necessary in order to achieve robust cAMP-stimulated adiponectin secretion as well as to maintain secretion over longer time-periods (recruitment of new releasable vesicles; [5]). Thus, the temperature-dependence of white adipocyte exocytosis described in this study elucidates controlling mechanisms involved in vesicle recruitment needed for augmentation and long-term maintenance of adiponectin release.

Our findings are summarised schematically in Fig. 6. We propose that vesicles ready for release may still undergo exocytosis in response to an elevation of cAMP even after cooling, whereas a drop in temperature abrogates more upstream events that are reliant on Ca²⁺. ATP-dependent processes are involved in maintenance of exocytosis in a magnitude of secreting cell types (reviewed in [2]) and this concept can be extended to adipocytes. We have shown that cAMP-triggered release of a readily releasable vesicle pool is unaffected by ATP-deprivation, whereas the Ca²⁺-dependent augmentation of secretion requires the intracellular presence of MgATP [5]. It is conceivable that the effects of cooling presented here in part reflects the necessity of ATP-hydrolysis in order to provide energy for recruitment of new releasable vesicles as well as for phosphorylation of proteins involved in control of exocytosis [2]. However, the rate of enzymatic reactions usually decreases by ~7% for every degree the temperature drops. Thus, the Q₁₀ >3 indicate the existence of additional temperature-dependent processes [15].

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Fig 6. Proposed model of cooling effects on white adipocyte exocytosis.

White adipocytes release adiponectin containing vesicles belonging to two functionally distinct populations. cAMP stimulates release of vesicles residing in a readily releasable pool in a temperature-independent manner. The Ca²⁺-dependent augmentation of secretion is reduced/abolished by cooling. Intracellular ATP is necessary for the Ca²⁺ effect. See text for more details.

https://doi.org/10.1371/journal.pone.0119530.g006

Our observations in adipocytes are analogous to findings in pancreatic beta-cells [8] and chromaffin cells [7] where secretion of release competent vesicles are unaffected by temperature but vesicle recruitment is reduced by cooling. Also in pituitary melanotrophs, cooling is without effect on a first exocytotic burst corresponding to a pool of releasable vesicles while an ensuing slow phase show great temperature sensitivity [9]. It is interesting that the early and late steps of exocytosis are similarly (un)affected by temperature in adipocytes and other endocrine cell types considering that the pathway of stimulus-secretion coupling differs.

The finding that Ca²⁺ acts as a regulator and not a trigger of adipocyte exocytosis is similar to how mast cell release of inflammatory mediators is controlled. An elevation of cytoplasmic Ca²⁺ is insufficient to trigger exocytosis in this cell type but Ca²⁺ can potentiate secretion triggered by other stimuli, such as the non-hydrolysable GTP analogue GTPgammaS [18]. The shown participation of Epac in white adipocyte exocytosis [5] suggests an involvement of GTP-dependent exocytotic steps. It is thus an exciting possibility that white adipocyte exocytosis, in analogy to what has been shown in mast cells [18] and in some neuroendocrine cell types [19,20], is triggered by GTPgammaS. Interestingly, the magnitude of small G-protein activation has been shown to depend on Ca²⁺ (reviewed in [21]) and hydrolysis of ATP has been suggested to be essential for robust GTPgammaS induced stimulation of exocytosis [19,20].

Our calculated Q₁₀ value of 3.3 and the energy of activation (53 kJ mol^-1) derived from the capacitance recordings are lower than that reported in pancreatic beta-cells (5 and 145 kJ mol^-1 respectively) [8]. However, insulin secretion is known to be a highly temperature sensitive process, in part due to the rather small size of the readily releasable vesicle pool [8]. Subcutaneous adipose tissue has been suggested to be more important than visceral depots for circulating levels of adiponectin [22,23]. In a physiological context, it seems rational that secretion in subcutaneous adipocytes is less sensitive to cooling since it is situated just beneath the dermis and may accordingly be exposed to low ambient temperatures.

Taken together, the results of this study show that white adipocyte exocytosis/adiponectin secretion encompasses energy-dependent processes. It is conceivable that control of vesicle recruitment signifies an essential mechanism by which the white adipocyte adjusts secretion of adiponectin in response to increased physiological requirements. The fact that adiponectin levels are reduced in obese individuals combined with observations that elevated levels provide protection towards development of type-2 diabetes in normal-weight individuals [24] emphasises the importance of understanding mechanisms controlling release of this adipokine.

Acknowledgments

We thank Prof Patrik Rorsman for reviewing of the manuscript.

Author Contributions

Conceived and designed the experiments: AMK MFE CSO. Performed the experiments: AMK MFE. Analyzed the data: AMK MFE CSO. Contributed reagents/materials/analysis tools: CSO. Wrote the paper: CSO.

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[4] Google Scholar

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View Article
PubMed/NCBI
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[6] View Article

[7] PubMed/NCBI

[8] Google Scholar

[ref3] 3. Bogan JS, Lodish HF. Two compartments for insulin-stimulated exocytosis in 3T3-L1 adipocytes defined by endogenous ACRP30 and GLUT4. J Cell Biol 1999; 146: 609–620. pmid:10444069
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PubMed/NCBI
Google Scholar

[10] View Article

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[ref4] 4. Bose A, Guilherme A, Huang S, Hubbard AC, Lane CR, Soriano NA, et al. The v-SNARE Vti1a regulates insulin-stimulated glucose transport and Acrp30 secretion in 3T3-L1 adipocytes. J Biol Chem 2005; 280: 36946–36951. pmid:16131485
View Article
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Figures

Abstract

Introduction

Material and Methods

Cell culture and differentiation

Electrophysiology

Measurements of intracellular Ca2+ concentrations

Studies of secreted adiponectin

Data analysis

Results

The effect of temperature on 3T3-L1 adipocyte exocytosis

Q10 values of exocytosis

Effects of cooling on 3T3-L1 adipocyte adiponectin secretion

Discussion

Acknowledgments

Author Contributions

References

Measurements of intracellular Ca²⁺ concentrations