The oscillatory behavior of the system

We used the deterministic version of the model proposed by Gonze and coworkers [9] as the starting point for our study. First, we characterized the effect of the R-dependent translation on the system dynamics, using similar conditions (i.e.: k_s = 2 h⁻¹) to those previously reported [9, 29]. In order to do this, we studied the dynamical behavior of the system when the R-dependent translation has a small contribution to the overall PER translation, that is, when the V_r and K_r parameters are very small compared to the k_s value (i.e.: k_s = 2 h⁻¹, K_r = 10⁻² nM and V_r = 10⁻² nM h⁻¹).

Fig. 2, A and B, shows the time evolution of M, P_n and P_t for nH = 4 and nH = 1, respectively. The system displayed sustained circadian oscillations in all the variables, regardless of the assumption of a cooperative (n = 4) or a non-cooperative (n = 1) repression of per transcription. The amplitudes of such oscillations were dramatically lower in the non-cooperative model and power spectrum analysis (S1 Fig. A and B in S1 File) revealed major differences in the oscillation period (τ between the cooperative τ ≈ 22.3 h) and the non-cooperative (τ ≈ 27.7 h) cases. S1 Fig. C and D in S1 File shows a parametric plot of the concentrations of M vs P_t, revealing a limit cycle trajectory, which was obtained regardless of the initial conditions for both cooperative and non-cooperative models. These results indicate that for a small contribution of the R-dependent translation to the overall translation, the system ruled by Equations 1 to 5 reaches a regime of self-sustained oscillations, regardless of whether the model assumes cooperativity or not. Thus, assuming a cooperative repression of per transcription favors the periodic behavior of the system, increasing the amplitude of the oscillations but shortening the period length, in agreement with the results of previous reports [6]. For the cooperative case (nH = 4), we have not found major differences in the period (τ ≈ 22.3 h) or the amplitude (a) of the oscillations between the model under the conditions presented in Fig. 2 A (k_s = 2 h⁻¹, K_r = 10⁻² nM and V_r = 10⁻² nM h⁻¹) and the model under the conditions corresponding to the original model (i.e.: k_s = 2 h⁻¹, K_r = 0 nM and V_r = 0 nM h⁻¹; [9], S2 Fig. A in S1 File). For the non-cooperative case (nH = 1), on the other hand, we found that the oscillation amplitude significantly decreased when the R-dependent translation was considered (S2 Fig. B in S1 File) while the period is practically unaffected (τ ≈ 27.7 h). Together, these results revealed that a small contribution of the R-dependent translation to the overall PER translation produced minor changes in the period of the molecular clock as compared to those obtained with the original model [9]. These conclusions are further supported by the evidence emerging from the comparison between the mRNA concentration ([M]) and the K_r value (0.01 nM). Fig. 2 A shows that the [M] value at the peak is much bigger than the K_r value ([M] ≈ 1.6 nM >> K_r) and therefore the Michaelian term can be reduced to a constant term, approximately equal to V_r. The effective PER translational kinetics (that is, the sum of the R-dependent and the R-independent terms) can be written as (k_s + Z)[M], with $Z=\frac{V_r}{[M]}$ << k_s, which leads to an effective translational kinetics very close to that considered in the original paper [9]. On the other hand, when [M] becomes small and gets close to the K_r value (∼ 0.01 nM at the trough), the Michaelian term can be reduced to $\frac{V_r[M]}{2K_r}~\frac{[M]}{2}$ and the effective kinetics can be written as $(ks+\frac{1}{2})[M]$, which is equivalent to changing the k_s value from 2 h⁻¹ to 2.5 h⁻¹ in the original model. The impact of such a change on the period length will certainly depend on the sensitivity of the model regarding the k_s variation; our numerical results revealed that the inclusion of the Michaelis-Menten term (R-dependent mechanism), with those specific K_r and V_r values, only perturbs slightly the dynamics of the system, generating a first order effective PER translation kinetics very similar to that of the original model. This explains why for nH = 4 neither the period nor the amplitude changed drastically in the model considered in Fig. 2 A, as compared with the original [9]. For nH = 1, (Fig. 2 B and S2 Fig. B in S1 File), the [M] value (∼ 0.8 nM at the peak and ∼ 0.5 nM at the trough) is, at least, fifty times bigger than the K_r value. In this case we could also consider that [M ] >> K_r and infer that the effective translational kinetics is ultimately described by a first order law, very similar to the one presented in the original model. Therefore we would not expect any significant changes in the dynamical properties of the model considered in Fig. 2 B as compared with the original model. Although this seems to be true for the period length, our numerical results revealed that the oscillation amplitude changed significantly (∼ 17—19%) in our model as compared with the original one. This observation highlights, on one hand, that it is not always possible to intuitively predict the dynamical behavior of a system like the one presented here. On the other hand, this observation reveals that the changes in amplitude produced by the contribution of the R-dependent translation were indeed dependent on the Hill coefficient, with the differences being more evident for nH = 1 than for nH = 4. It is noteworthy that the Michaelis-Menten term, under the conditions of Fig. 2 B, impacts more on the peak-to-trough amplitude than on the period length.

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Figure 2. Sustained oscillations generated by the PER model.

Temporal evolution of the variables M, P_n, and P_t, which represent the Per mRNA, nuclear PER and total PER, respectively. Repression of per transcription was assumed as a cooperative (A) or a non-cooperative (B) process. Parameter values are those listed in S1 Table in S1 File, except for the translation-related parameters, which, for this figure, acquired the following values: k_s = 2 h⁻¹, K_r = 10⁻² nM and V_r = 10⁻² nM h⁻¹.

https://doi.org/10.1371/journal.pone.0115067.g002

To further study the changes in period and amplitude when the R-dependent mechanism has a bigger contribution to the overall translational process, in the next section we show a detailed numerical characterization of the dynamics of our model for different combinations of k_s, V_r and K_r parameter values.

Numeric characterization of oscillatory behavior as a function of the parameters related with PER translation

In order to study the dependence of the system dynamics on the translation-related parameters (k_s, V_r and K_r), we numerically characterized the period (τ) and the amplitud (a) of the per mRNA (M) and total PER (P_t) oscillations as functions of these parameters (Fig. 3, Fig. 4 and S5–S6 Fig. in S1 File).

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Figure 3. Dependence of the period (τ) with k_s, V_r and K_r, for nH = 4.

(A) The τvalues are shown in pseudocolor within the K_r-V_r-parameter space for different values of the k_s parameter from 0 (left) to 2 h⁻¹ (right). Black areas indicate zones in which there are damped oscillations. A white dotted line was added in order to emphasize the transition between the two dynamical behaviors observed in our model. Note the changes in the oscillatory domain and the τ length with k_s (the color key for τ is at the bottom of the plots). (B) An inset of the K_r-V_r-space shown in panel A for k_s = 2 h⁻¹ (right) shows the subtle changes in τ. Note that the key color was rescaled in order to display the differences in more detail. (C) Dependence of τ (red line) with V_r for a fixed value of K_r = 0.01 nM). Black line shows the pseudo-period value for damped oscillations. See the text for further details.

https://doi.org/10.1371/journal.pone.0115067.g003

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Figure 4. Dependence of the period (τ) with k_s, K_r and V_r for nH = 1.

(A) The τ values are shown in pseudocolor within the K_r-V_r-subspace for different values of the k_s parameter, from 0 (left) to 2 h⁻¹ (right). Infradian (τ ≥ 28 h) are predominant for k_s values < 2 h⁻¹. For each k_s value, the range of τ values is narrower than those observed for nH = 4 (the color key for τ is at the bottom of the plot). Black areas indicate zones in which there are damped oscillations. A white dotted line was added in order to emphasize the transition between the two dynamical behaviors observed in our model. (B) An inset of the K_r-V_r-space shown in panel A for k_s = 2 h⁻¹ (right) shows how τ changes within the circadian range; nevertheless the extent of τ change is smaller than that observed for nH = 4. Note that the key color was rescaled in order to display the differences in more detail (C) Dependence of τ (red line) with V_r for a fixed value of K_r = 0.01 nM. The black line shows the pseudo-period value for damped oscillations.

https://doi.org/10.1371/journal.pone.0115067.g004

For the cooperative model (nH = 4), Fig. 3 A shows the phase diagrams in the K_r-V_r subspace, in which the τ values are shown in pseudocolors and each diagram corresponds to a different k_s value. The k_s variations were introduced in order to analyze the relative importance of the R-independent translation versus the R-dependent mechanism. We have systematically varied the k_s values ranging from 0 to 2 h⁻¹ and representative diagrams for specific k_s values are presented in Fig. 3 A. For all the k_s values considered, two major dynamic behaviors were observed depending on the V_r and K_r values: a region where the system oscillated in a sustained way (colored regions) and another region where the system decayed to a fixed point in an oscillatory manner (damped oscillations, black regions).

When the only mechanism involved in the PER translation was the R-dependent one (k_s = 0 h⁻¹), we found a small domain within the V_r-K_r subspace in which the system oscillated self-sustainedly with different τlengths, depending on the V_r-K_r parameter values. Circadian and close-to-circadian oscillations (green) emerged only in a region surrounded by the domains of ultradian (blue) and infradian (yellow to red) oscillations. In order to quantify the strength of saturation for the PER translation process taking place via the R-dependent mechanism, we numerically calculated the saturation index (I) values for each K_r-V_r combination within the oscillatory domain ([38], see details in the S1 File). S3 Fig. A in S1 File shows that a high proportion of the oscillatory domain presents small I values (between 0 and 0.5) and a small region exhibits large I values (between 0.5 and 0.8). However, the I values were never bigger than 0.8. We did not observe any correlation between the I values and the oscillation period. For K_r >>> [M], the I values were small (i.e.: right-top corner of S3 Fig. A in S1 File) and the system with an R-dependent translation (k_s = 0 h⁻¹) could trivially have the same behaviour than the original model introduced by Gonze ([9], k_s = 2 h⁻¹; V_r = 0 nM h⁻¹), as long as V_r = 2K_r (green straight line plotted in S3 Fig. A in S1 File). Taken together, these results revealed that a PER translation process taking place exclusively via an R-dependent mechanism (Michaelis-Menten kinetics; k_s = 0 h⁻¹) was sufficient to obtain self-sustained oscillations within a specific V_r-K_r domain; within that domain it was possible to obtain different degrees of saturation by tuning the K_r and V_r parameter values.

When k_s > 0 h⁻¹, self-sustained oscillations emerged in V_r-K_r domains that increased with k_s and spread out from the infradian oscillation region. Between k_s = 0.15 h⁻¹ and 0.16 h⁻¹ the system exhibited a transition from a regime dominated by damped behavior to a regime dominated by self-sustained oscillations. In the latter regime, the shape of the oscillatory domain remained practically unchanged as k_s increased, with only slight variations in the periodicity. We observed that as k_s increased, the domains of infradian oscillations decreased in size. In particular for k_s = 2 h⁻¹ the infradian region disappeared and the subspace was dominated by circadian and close-to-circadian behavior. Fig. 3 B is an inset of the diagram shown in Fig. 3 A for k_s = 2 h⁻¹, which does not maintain the original color ranges exhibited in the corresponding full diagram because we wanted to highlight the subtle τ differences in more detail. This figure reveals that, for a fixed value of K_r, an increase in the V_r value produced a shortening of the τ length. This dependence of τ on the V_r value was observed regardless of the k_s values examined, which was clearly represented by the pseudocolor variation in each map of Fig. 3 A, going from red (long τ to blue (short τ). We did not observe, for any of the specific k_s and K_r values considered, any alteration of the pseudocolor gradient as V_r grew (that is, τ codified in red > τ codified in orange > τ codified in yellow > τ codified in green > τ codified in blue). This information can be also observed in Fig. 3 C (red line), in which we have plotted the τ values as a function of the V_r for a fixed value K_r = 0.01 nM. This figure also includes the pseudo-period values of the damped oscillations (black line) present in the black region of the diagram shown in Fig. 3 A for k_s = 2 h⁻¹ (see below). S3 Figs. B–D in S1 File show the I values within the K_r-V_r-diagrams for the other k_s values shown in Fig. 3 A. We observed that the proportion of the oscillatory domain presenting large I values (i.e.: I ∼ 1) increased as we increased the k_s value from 0.15 h⁻¹ (S3 Fig. B in S1 File) to 0.16 h⁻¹ (S3 Fig. C in S1 File), which means that the R-dependent translation becomes highly saturated in that region. This is likely to be related to the transition observed in the K_r-V_r diagrams, which changed from a regime dominated by damped behavior to a regime dominated by self-sustained oscillations (including the region of small K_r and V_r values). Nevertheless, when we further increased the k_s value from 0.16 h⁻¹ (S3 Fig. C in S1 File) to 2 h⁻¹ (S3 Fig. D in S1 File) we observed that the region with small I values (between 0 and 0.5) becomes increasingly bigger. This is consistent with the fact that an increase of k_s leads to an increase of the effective kinetics of PER translation, which reduces the overall mRNA concentration available to be translated by the R-dependent mechanism.

Fig. 4 shows a similar analysis but for the non-cooperative model (nH = 1). In this case (Fig. 4 A), for all the k_s values studied, the domains where self-sustained oscillations emerged were very small compared with those obtained with the cooperative model (Fig. 3 A). Furthermore, in these domains only long circadian or infradian periods were observed. For k_s = 2 h⁻¹, the whole oscillatory domain presented circadian oscillations with τ values ≈ 27—28 h: there was a decrease in the period length as V_r increased, but for an identical increase in the V_r parameter, the τ length decreased less when nH = 1 than when nH = 4 (compare Fig. 3 B and Fig. 4 B). Indeed, no ultradian periods were observed. Fig. 4 C also shows the changes in τ as V_r increases, for a fixed value of K_r = 0.01 nM (red line). Although the period appeared to be constant, it actually decreased slowly. The pseudo-period values (black line) corresponding to the damped oscillations (black region of the diagram shown in Fig. 4 A for k_s = 2 h⁻¹) have also been included. The analysis of the I values for nH = 1 (S3 Fig. E–F in S1 File) is similar to that for nH = 4. The only difference is that, for k_s = 0 h⁻¹ and k_s = 0.9 h⁻¹, the system was not saturated regardless of the K_r-V_r combination tested. This means that the Michaelis-Menten kinetics can be approximated by a first order kinetics, and the effective translational kinetics (sum of R-dependent and R-independent) could be written as $k_s^{\prime }[M]$, with $k_s^{\prime }=(k_s+V_r/K_r)$, which would give trivially the same result than the original model with a change in k_s.

The pseudo-period values shown in Fig. 3 C and Fig. 4 C were included in order to show that beyond the self-sustained oscillatory domain the system displays transient damped oscillations before reaching the steady state. Using linear stability analysis we have confirmed the existence of Hopf bifurcations along the transition lines between the non-oscillatory (dampened) and oscillatory domains (white dotted lines in Fig. 3 A and Fig. 4 A; see the Supporting Information and S4 Fig. in S1 File). From the damped oscillations it was possible to measure pseudo-period values by numerically computing the average duration (time) between two consecutive peaks of the damped oscillations observed in PER concentration [39]. Near to the Hopf bifurcation point, these damped oscillations can exhibit pseudo-period values within the same range than the τ values corresponding to self-sustained oscillations on the other side of the transition lines.

The peak-to-trough amplitude (a) values for M and P_t were also calculated, codified in a pseudocolor key, and represented in phase diagrams in the V_r-K_r parameter subspace, both for cooperative and non-cooperative models (S5 Fig. and S6 Fig. in S1 File, respectively). In these figures we show the a diagrams for M and P_t at two different k_s values, k_s = 0 h⁻¹ and k_s = 2 h⁻¹. We observed that, regardless of the value of the Hill coefficient considered, the amplitude range of M was larger for k_s = 0 h⁻¹ than for k_s = 2 h⁻¹, even when the latter produced a wider oscillatory domain. On the other hand, the amplitude range of P_t for nH = 4 remained the same for both values of k_s, whereas for the non-cooperative model (nH = 1) the amplitude ranges of P_t changed slightly between the two k_s values, being greater for k_s = 0 h⁻¹. In addition, we also observed that a cooperative repression of per transcription always enlarged the oscillation amplitude, regardless of the values of k_s explored, which is in agreement with the results shown previously in Fig. 2. As a final remark it is important to note that the results and conclusions showed in this section hold as long as the values for the rest of the parameters of our model are the same as those listed in S1 Table in S1 File.

Characterization of the time lag between the per mRNA and PER protein expression profiles

A still open question about the molecular circadian clock is how the delay (denoted by δ) between the expression of per mRNA and protein is achieved. Long time lags between the mRNA and protein expression profiles of PER have been observed experimentally: the phase of PER expression is delayed about 6–8 h relative to the mRNA [40–42]. This time lag was mainly associated with the stability of mRNA and proteins, post-translational modifications, dimerization processes, nuclear translocation, etc [42–45]. However, it was also suggested that the translational regulation may also be contributing to the observed delays [40, 46]. To test this hypothesis, we have computed the time delay (δ) between the maximum expressions of per mRNA and nuclear PER (denoted by δ_n) and between the maximum expressions of per mRNA and total PER (denoted by δ_t) obtained with our model, for different k_s, K_r and V_r values. These δ values are represented in pseudocolor within the K_r-V_r subspace for the cooperative and non-cooperative cases (Fig. 5 and S8, S14 and S16 Figs. in S1 File).

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Figure 5. Analysis of the delays when PER translation is achieved by a single kinetic mechanism.

Total delay (A) and nuclear delay (B) diagrams within the V_r-K_r parameter subspace, for a cooperative model in which k_s = 0 h⁻¹. Total delay profile (C) and nuclear delay profile (D) diagrams as functions of the period τ. Each color line represents a fixed value of K_r, as follows: red = 100 nM; green = 10 nM; blue = 1 nM and magenta = 0.14 nM. For a better comparison we have included the profile δ_t versus τ for the model with a translational process achieved only by the R-independent mechanism (V_r = K_r = 0), which follows a first-order kinetics (black line). All these curves increase monotonically as τ increases. Figs. 5 E and F represent, respectively, the normalized total delay and nuclear delay diagrams within the K_r-V_r-subspace. The color key for each K_r-V_r-diagram is shown at the bottom of each plot. In those diagrams a white dotted line was added in order to emphasize the transition between the two dynamical behaviors observed in our model.

https://doi.org/10.1371/journal.pone.0115067.g005

We started by examining the delays obtained with the original model, which assumed a first-order kinetics for the translational process [9]. This condition was achieved in our model by setting V_r = 0 nM h⁻¹, k_s = 2 h⁻¹ and nH = 4. Under these conditions, the δ_n value was about 8.39 h and the δ_t value was about 5.11 h (S7 Fig. A in S1 File, arrows). S7 Fig. A in S1 File also shows a further characterization of the delays dependence on the k_s parameter when the translational kinetics followed a first-order rate and nH = 4: a decrease of k_s led to an increase of both δ_n and δ_t values. A similar profile was found for the variation of the oscillation period (τ) with k_s (S7 Fig. C in S1 File). Furthermore, a monotonic relationship between delays and period was the prevailing trend observed for nH = 4 (S7 Fig. E in S1 File), in which the longer the period, the greater the delay. Minor exceptions to this behavior were found at each end of the δ_t profile (i.e.: for very short or very long periods), where the δ_t values have changed little from one period to the other. We also observed a slowdown of the δ_n profile for very long periods. For nH = 1, the period monotonically decreased with k_s, just as for nH = 4 (S7 Fig. D in S1 File). Nevertheless, the relationship between delays and k_s was not monotonic (S7 Fig. B in S1 File), which clearly impacted on the delays-versus-period profiles: delays passed through a maximum as a function of the period length (S7 Fig. F in S1 File).

When PER translation was achieved only through an R-dependent mechanism (i.e.: k_s = 0 h⁻¹) and nH = 4, the delay values depended on the V_r and K_r values (Fig. 5 A and B). For a fixed value of K_r, delays tended to decrease as V_r grew (S9 Fig. A and B in S1 File, rhombs), which qualitatively resembled the dependence of delays with k_s previously observed in Fig. 6 A. However, a change in the V_r parameter produced smaller changes in the delays than those arising from similar changes in k_s for the original model because the presence of the K_r parameter in the Michaelis-Menten mechanism produced opposing effects on the delays, which tend to increase as K_r grows (S9 Fig. C and D in S1 File, rhombs). Conversely, when nH = 1 the dependence of delays with V_r or K_r was not monotonic (S8 Fig. A and B in S1 File; see also S9 Fig. in S1 File, circles).

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Figure 6. Profiles of the period (τ) as a function of several non-translation related parameters of the model.

Dependence of the period (τ) on: the maximum rate of per mRNA synthesis, v_s (A); the Michaelis constant for per mRNA degradation, K_m (B); the maximum rate of PER degradation, v_d (C) and the Michaelis constant for PER degradation, K_d (D). We compared a model in which PER translation take place by an R-independent mechanism (first-order kinetics; k_s = 1.48 h⁻¹; K_r = 0 nM and V_r = 0 nM h⁻¹; black curves) and a model in which PER translation take place by an R-dependent mechanism (Michaelis-Menten kinetics; k_s = 0 h⁻¹; K_r = 0.3 nM and V_r = 2.09 nM h⁻¹; gray curves). Arrows indicate the values of the control parameters listed in S1 Table in S1 File.

https://doi.org/10.1371/journal.pone.0115067.g006

Figs. 5 C and D (see also S11 and S12 Figs., B and C, in S1 File) show the relationships between delays and period for k_s = 0 h⁻¹ and nH = 4, which were qualitatively similar to those shown in S7 Fig. E in S1 File: the prevailing behavior observed was a monotonic relationship between delays and period, with slight deviations from this trend at each end of the δ_t profile and in the region corresponding to very long τ of the δ_n profile.

These results led us to ask how the delays arising from each translational mechanism considered here (i.e.: R-dependent vs R-independent) behave for a given (fixed) period of the molecular clock. In order to study this, we searched into the K_r-V_r parameter space shown in 5 Fig. (panels A and B) for a set producing oscillations with τ = 22.3 h, which is the period value reported for the original model [9]. For nH = 4 we found several combinations of the V_r and K_r parameter values that gave rise to oscillations with τ = 22.3 h, most of which yielded δ_n and δ_t values equal or longer than those obtained for the original model (S10 Fig. in S1 File). Indeed, for δ_t this result was independent of the τ fixed for comparison (5 Fig. C; see also S11 Fig. B and C in S1 File). Notice that δ_t becomes longer as the PER translation becomes more saturated (compare the K_r isolines shown in 5 Fig. C with their I values shown in S3 Fig. A in S1 File).

Conversely, the δ_n versus τ profile exhibited two different behaviors: for oscillations with periods longer than 20 h the δ_n values obtained with an R-dependent translational mechanism were equal or longer than those obtained with an R-independent mechanism. On the other hand, for periods shorter than 20 h the R-dependent mechanism produced equal or shorter δ_n’s than those obtained with an R-independent mechanism (5 Fig. D; S12 Fig. B and C in S1 File). In contrast, for nH = 1 the relationship between delays and period was not monotonic (S8 Fig. C and D in S1 File see also S13 Fig. A and B in S1 File) and delays obtained from a model with an R-independent mechanism of translation (i.e.: V_r = 0 nM h⁻¹) were equal or longer than those obtained with an R-dependent mechanism (i.e.: k_s = 0 h⁻¹), irrespectively of the τ fixed for comparison.

To further clarify the relationship between delays (δ_t and δ_n) and period (τ), we have normalized the delay values by the period length and characterized the normalized delay sensitivity regarding the V_r and K_r values (5 Fig. E and F). For nH = 4 this analysis revealed changes in the delays that were independent of the period length, or, in other words, exclusively dependent on the parameters V_r and K_r. This effect seemed to be enhanced by the cooperative repression of per transcription (i.e.: nH = 4), because for nH = 1 the normalized delay values were more homogeneous than for nH = 4 (compare 5 Fig. E and F with S8 Fig. E and F in S1 File).

From these observations we can conclude that a translational regulation process which generates a PER translation rate of Michaelis-Menten type (R-dependent) will contribute with equal or longer delays (δ_t and δ_n) to the molecular clock than a mechanism in which the PER synthesis rate grows linearly with the per mRNA concentration (R-independent), as long as nH = 4 and the K_r-V_r parameter combination generates oscillations with periods longer than 20 h. We also observed that a saturated kinetics of PER translation enlarges the δ_t values. These conclusions hold as long as the values for the rest of the parameters of our model are the same as those listed in S1 Table in S1 File.

Regarding the effect of the Hill coefficient (nH), we conclude that a non-cooperative transcriptional repression of per (nH = 1) tended to enlarge the delay values, irrespectively of the functional form of the translation kinetics considered here. For instance, for the original model (K_r = 0 nM, V_r = 0 nM h⁻¹, k_s = 2 h⁻¹), a non-cooperative repression of per transcription resulted in longer delays (δ_n = 8.77 h and δ_t = 6.66 h) than those obtained with the cooperative model (nH = 4) (δ_n = 8.39 h and δ_t = 5.11 h) (S7 Fig. in S1 File, arrows) and the same qualitative result was obtained when the PER synthesis was achieved only by the R-dependent mechanism (S9 Fig. in S1 File). Furthermore, a non-cooperative transcriptional repression of per (nH = 1) tended to confine the diversity of delays observed within the K_r-V_r domain of self-sustained oscillations.

In addition, when we considered that PER translation was achieved by a combination of the two mechanisms proposed in our model (R-dependent plus R-independent), we observed that, by increasing k_s, the delay distributions (both, the absolute and the normalized delays) became narrower than those observed for k_s = 0 h⁻¹ (S14 and S15 Figs. in S1 File). This result was independent of the nH value considered here (S16 and S17 Figs. in S1 File). As a final remark we note that the observed nontrivial phase shifts (delays) require of the coupling of the various components of the circadian clock. If, for instance, a sinusoidal per mRNA were used as an input signal, the introduction of a Michaelian term would not per se lead to a change of the delays. This can be seen by considering the uncoupled Eq. (2) and noticing that the maxima and minima of P₀ coincide with the zeroes of the expression on the right-hand side, whose location is not changed by the presence of the Michaelian term.

Since assuming nH = 4 favored the periodic behavior of the system, facilitated the saturation of the R-dependent translational mechanism, increased the amplitude of mRNA oscillations and also increased the delays sensitivity to changes in the V_r and K_r values, we have continued our study considering that the repression of per transcription is a cooperative process.

Influence of other parameters on the period of oscillations

Previous studies have reported that the phosphorylations, protein stability and nuclear translocations are major period determinants in the molecular circadian clock [42, 44]. For instance, Goldbeter [6] has shown that the maximum rate of PER degradation is a key control parameter of the model that he proposed. On the basis of these antecedents, we studied the sensitivity of the oscillatory behavior of our model to changes in their parameter values. In particular, we were interested in determining whether or not the kinetics of PER translation modifies the sensitivity of the oscillatory behavior to changes in parameter values. We compared, therefore, the influence of the parameter variation on τ when the PER translation is assumed to occur by a single kinetic pathway, either with Michaelis-Menten kinetics (R-dependent mechanism; k_s = 0 h⁻¹) or with first-order kinetics (R-independent mechanism; V_r = 0 nM h⁻¹ and K_r = 0 nM ). In order to conduct the comparison between these two conditions we established two similar systems, that is, systems exhibiting sustained oscillations with the same τ value, whose only difference was the kinetics of PER translation. Starting from our ‘master’ model, we looked for these two similar systems by exploring and specifying either the K_r-V_r subspace in one case, or the k_s subspace in the other, while the rest of the parameter values were maintained as presented in S1 Table in S1 File. In constant darkness, the τ observed in the wild type (WT) Drosophila is close to 24 h (i.e.: τ = 23.8 h, [25]). Consequently, we decided to set the K_r and V_r values in one case, and the k_s in the other case, in such a way that the τ value, in each system, was 23.8 h.

Therefore, we compared a model in which PER translation takes place by an R-independent mechanism (first-order kinetics; k_s = 1.48 h⁻¹; K_r = 0 nM and V_r = 0 nM h⁻¹); and a model in which PER translation takes place by an R-dependent mechanism (Michaelis-Menten kinetics; k_s = 0 h⁻¹; K_r = 0.3 nM and V_r = 2.09 nM h⁻¹, white dot within the K_r-V_r diagram shown in S3 Fig. A in S1 File, I ∼ 0.72). After establishing either the K_r and V_r or the k_s values, we maintained them fixed. Then, we proceeded to determine how τ is altered when each parameter (other than k_s, V_r, K_r and nH) is varied (one at a time) by ± 100% of their original value (S1 Table in S1 File). Results are shown in Fig. 6 and S18 Fig. in S1 File.

The black curves in Fig. 6 and S18 Fig. in S1 File correspond to the model in which the PER translation occurred by an R-independent mechanism, while the gray curves correspond to the model in which the PER translation occurred by an R-dependent mechanism. Arrows in 6 Fig. indicate the initial values of the control parameters (S1 Table in S1 File). For all the cases shown in Fig. 6 we observed that the τ profiles changed significantly as a consequence of the functional form of the translation kinetics. On the other hand, the cases shown in S18 Fig. in S1 File did not exhibit significant changes between the two conditions tested here.

Fig. 6 A shows the dependence of τon the Michaelis constant for per mRNA synthesis (v_s). The model with an R-independent mechanism (black curve) exhibited a τ profile that initially decreased, v_s value increased. On the other hand, in the model with Michaelis-Menten kinetics (gray curve), the τ length increased monotonically as v_s increased. Leloup and Goldbeter [38] defined two different types of sensitivity, one related to the extent of τ variation derived from changes in the control parameter (i.e.: influence of each parameter on the period), and the other related to the size of the oscillatory domain (i.e.: higher sensitivity when the window for oscillations is relatively narrow). For simplicity, here we named these two sensitivities as type I and type II, respectively. On the basis of this information we observed that an R-dependent translation decreased the type I sensitivity (i.e.: the slope of the black curve was steeper than the slope of the gray curve), but slightly increased the type II sensitivity (notice that the v_s range in which oscillations emerged was quite narrow when the model assumed an R-dependent translation).

Fig. 6 B shows the dependence of τ on the Michaelis constant for per mRNA degradation (K_m). When PER translation was achieved by an R-independent mechanism (black curve), the τ initially decreased, then passed through a minimum and finally increased as the K_m value increased. When PER translation was achieved by an R-dependent mechanism (gray curve), the τ profile monotonically decreased as K_m increased. In this case, an R-dependent mechanism decreased the type I sensitivity (the absolute value of the gray-curve slope is smaller than the black curve slope) and increased the type II sensitivity (notice that the black curve continues to grow for K_m > 0.45 nM).

Thus, for the v_s and K_m parameters, which are important to establish the steady levels of the per mRNA, the two types of sensitivity changed similarly. Interestingly, when v_s and K_m are control parameters and the PER translation follows an R-dependent mechanism, the decrease of the type I sensitivity can be interpreted as if the synthesis and degradation of per mRNA would have partially lost power to influence the oscillation period.

Fig. 6 C shows the dependence of τ on the maximum rate of PER degradation (v_d). The τ profile derived from a model with an R-independent mechanism of PER translation (first-order kinetics; black curve), monotonically increased as v_d increased. This was in agreement with the profile reported by Goldbeter [6]. The τ values also rose as v_d increased for a model with an R-dependent mechanism (gray curve), but the range of v_d values for which sustained oscillations emerged was smaller and the slope of the curve was steeper than those observed with an R-independent translational mechanism. Therefore, a Michaelis-Menten based mechanism of PER translation (R-dependent) increased both type I and type II sensitivities.

Fig. 6D shows the dependence of τ on the Michaelis constant for PER degradation (K_d). The shape of the τ profiles did not change significantly between the two cases compared here: both profiles showed that τ monotonically decreased as K_d increased. Nevertheless, the τ profile derived from the model with an R-dependent translation (gray curve) exhibited a steeper slope than that obtained with the model where translation was achieved by an R-independent mechanism (black curve). In addition, the K_d range for which sustained oscillations emerged was shorter in the model with an R-dependent translation than in the model with an R-independent translation. Thus, in this case, an R-dependent mechanism increased the two types of sensitivity considered here. The results of Fig. 6 C and D revealed that an R-dependent translation increased the sensitivity of the oscillation period to changes in the PER degradation process.

Overall, these results suggest that an R-dependent translation leads to a change in the global sensitivity of the system, in which the synthesis and degradation of per mRNA becomes less decisive, but the PER degradation becomes more significant on the determination of the oscillation period. It is important to notice that these conclusions hold as long as the rest of the parameters (that is, those parameters not considered as control parameters for each sensitivity analysis) maintain the values listed in S1 Table in S1 File.

Testing our model with experimental results

After the exhaustive characterization of our model introduced in the previous sections, we proceeded to validate it by comparing its results with experimental data. In flies, Lim and coworkers [25] reported a Drosophila strain which has mutated a gene named twenty four (tyf). This gene codifies for a protein (TYF) involved in the regulation of per mRNA translation. It seems that TYF forms a complex with PABP (a RNA-binding protein which binds to the poly(A) tail of mRNAs) and ATAXIN-2 (ATX2), which ultimately interacts with the per transcript and activates PER translation in pacemaker neurons to sustain robust rhythms [25, 47, 48]. These results highlight the role of TYF-PABP-ATX2 complexes for the proper PER translation in the Drosophila neural clockwork. Importantly, tyf mutants expressed oscillating PER levels in lateral clock neurons but the peak-to-trough amplitude was lower than in WT flies. Furthermore, the period of behavioral rhythms in tyf mutants is longer than in WT flies. Since the TYF-PABP-ATX2 complex can associate with per mRNA and enhance the PER translation, we posit that TYF-PABP-ATX2 effects on PER translation kinetics could be well represented by a translational process occurring with a Michaelis-Menten kinetics. To test this hypothesis, we used a version of our model that considers that PER translation is only achieved by an R-dependent mechanism (Michaelis-Menten kinetics; k_s = 0 h⁻¹). Here it is worth noting that, although in some conditions our model produces oscillations with τ = 22.3 h (Fig. 2 A and S1 A and S2 A Figs. in S1 File), this τ value does not correspond to the period observed in the WT Drosophila [25]. As shown in previous sections, we used this τ value only as a benchmark to conduct the characterization of our new version of the core clock model. Nevertheless, in order to see whether our simple model can account, at least qualitatively, for the experimental facts described above, it is important to show that it leads to circadian oscillations with τ = 23.8 h, which is the τ reported by Lim and coworkers for the (WT) Drosophila maintained in constant darkness [25].

Setting the translation-related parameters values at k_s = 0 h⁻¹, K_r = 0.3 nM and V_r = 2.09 nM h⁻¹, (white dot within the K_r-V_r diagram shown in the S3 Fig. A in S1 File; I ∼ 0.72) and keeping the rest of the parameter values as listed in Table 1 in S1 File, our model exhibited sustained oscillations with τ = 23.8 h (Fig. 7 and Fig. 8 A). The tyf hypomorphic mutants can be modeled as a system with smaller values of the V_r than WT flies because this parameter is related to the concentration of the TYF regulator (see discussion). Under these conditions, our model mimics the τ observed in the two tyf hypomorphic mutants reported by Lim and coworkers [25] (Fig. 7). Notice that τ became longer as the V_r parameter decreased, which is qualitatively similar to the experimental results stated above. Another qualitative similarity between the theoretical and the experimental results was the decrease in the peak-to-trough amplitude of PER levels (Fig. 8 A) for the mutant flies. In addition, it is worth noting that our model predicted that the peak-to-trough amplitude a of the per mRNA for the mutants flies was lower than for WT flies, but the maximum levels of the per transcript were slightly higher (Fig. 8 A). By comparing the temporal evolution of the per mRNA and total PER obtained with our model for the parameter values used in Fig. 7, we could roughly predict the time delays δ_t for WT and mutant flies (Fig. 8 B). We observed that the δ_t (approximately 7.4 h) for the WT flies was shorter than those corresponding to the tyf^Δ and the tyf^e mutants (around 7.9 and 8.3 h, respectively, Fig. 8 B). These general and qualitative observations regarding the delay could be tested in future experiments.

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Figure 7. Comparison between theoretical and experimental results.

The dashed gray line shows the dependence of τ with the V_r parameter obtained with our model, for k_s = 0 h⁻¹, K_r = 0.3 nM h⁻¹ and nH = 4. The periods of behavioral rhythms in wild type (WT) flies and tyf mutants (tyf^e and tyf^Δ) are also indicated with black points and arrows, according to [25]. See the text for further details

https://doi.org/10.1371/journal.pone.0115067.g007

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Figure 8. Circadian oscillations and delays in WT and tyf mutant flies.

(A) Time evolution of the per transcript (solid lines) and total PER protein (dashed lines) in WT (black), tyf^Δ (dark gray) and tyf^e (light gray) flies. (B) Delay values (δ_t) of each fly strain. Results obtained with our model for k_s = 0 h⁻¹, K_r = 0.3 nM h⁻¹ and nH = 4. Dotted line was plotted only for clarity purposes. See the text for further details.

https://doi.org/10.1371/journal.pone.0115067.g008

PER translation by a single mechanism with Michaelis-Menten kinetics

Translational regulation is an important control point for shaping proteomes since it allows for more rapid changes in cellular protein concentrations than those achieved by transcriptional regulation [21, 49]. In mammalian cells, protein expression levels are primarily determined by regulation of translation and protein degradation [35]. In Drosophila, at least a 40% of the protein concentration is determined by these processes [50]. The kinetics of such biochemical processes are generally unknown and therefore, the choice of the kinetics laws and parameters to model these processes remains a challenge [44]. It is now recognized that the translation kinetics is a function of mRNA concentration as well as of other factors, such as the concentration of protein, tRNAs, ribosomes and translational regulators, among others. Similarly, protein degradation is also highly specific and tightly regulated. The involvement of multiple factors in the effective kinetics of both protein synthesis and degradation, suggests that these are nonlinear processes [34, 35]. In this regard, most of the previous models of the circadian clock assumed that the stationary concentration of total PER is mainly determined by its synthesis (considered as a first-order rate equation, dependent on the per mRNA levels) and degradation (modeled as a process that follows Michaelis-Menten kinetics) [6, 9, 29]. This description considers the nonlinearities related to the degradation process but not those related to translation. Although the assumption that the kinetics of PER synthesis follows a linear relationship with the amount of per mRNA has been satisfactory to model the molecular clock, we sought to determine how the clock dynamics is affected when nonlinearities in the translational process are included. Additionally, in recent years a growing number of reports have stressed the role of translational control mechanisms in the dynamics of circadian clocks [3, 20, 22, 23, 46]. In particular, several studies have pointed out that specific proteins act as enhancers of the Drosophila PER translation [25, 26, 47, 48]. According to these evidences, we considered that one simple way to take into account a nonlinear translational kinetics is by representing the PER translation as a process following a Michaelis-Menten kinetics. In addition to enhancing the global nonlinearity of the system without over-complicating the clock model, the saturable kinetics of Michaelis-Menten confers robustness to the biochemical oscillations by preventing the rates from increasing without limits [51]. Furthermore, a nonlinear saturable kinetics, such as the Michaelis-Menten, can roughly represent the kinetic consequence of a translational process regulated by one or multiple regulators acting either in a global and/or a specific fashion, which ultimately enhances the PER translation. Indeed, most models for the molecular clock have introduced nonlinearities (through a Michaelis-Menten kinetics) in other biochemical processes such as transcription, protein degradation and phosphorylation [43]. However the introduction of nonlinearities in the translational process has been rare. The only exception we know was the work of Kurosawa and Iwasa [52], which analytically proved (in circadian clock models with three or four variables) that the degree of saturation of enzyme kinetics affects the possibility of observing sustainable oscillations. They noticed that this effect varies systematically with the location of the reaction in the gene-protein network, and showed that large saturation indexes (I) for some reactions called in-loop (i.e.: PER translation, modification in the cytosol and transport to the nucleus) suppress the oscillation. Moreover, they also calculated the I values for each reaction step included in previous circadian clock models, and they showed that in those models the I values for in-loop reactions (like the PER translation) are small (between 0 and 0.5, see Fig. 5 within ref. [52]). We have numerically computed the saturation index for the PER translation reaction occurring through an R-dependent mechanism (see the Supporting Information and S3 Fig., A and E, in S1 File) and we also found that self-sustained oscillations emerged when the I values are small (between 0 and 0.5), which is consistent with the results of Kurosawa and Iwasa. Nevertheless, when nH = 4 our five-variable model showed that there is a small region within the K_r-V_r subspace in which self-sustained oscillations emerge even when the I values are larger than 0.5 (i.e.: I values between 0.5 and 0.8; S3 Fig. A in S1 File). This may suggest that in our five-variable model the saturation of PER translation does not necessarily imply an inhibition of the self-sustained oscillations.

Recently, the effect of Michaelis-Menten kinetics has been also discussed in the context of other cellular rhythms such as the activity of phosphofructokinase in glycolysis and cyclin-dependent kinases in the cell cycle [51], where it is shown and discussed how the Michaelis-Menten equation can be used to study the dynamical behavior of enzyme systems. Here we have shown that a PER translation process taking place exclusively via an R-dependent mechanism (Michaelis-Menten kinetics; k_s = 0 h⁻¹) was sufficient to obtain self-sustained oscillations within a specific V_r-K_r domain (Fig. 3 A). Ultradian, circadian and infradian rhythms were observed, where the specific period, amplitude and delays depended on the specific values of the V_r and K_r parameters (Fig. 3 A, Fig. 6 and S4 Fig., A and B, in S1 File). These oscillatory properties also depended on the nH value (Fig. 4 A and S6, S8, S9 and S13 Figs. in S1 File) and other parameters of the model, therefore, the conclusions stated here hold as long as the values for the rest of the parameters of our model are the same as those listed in S1 Table in S1 File.

Extended clock models that incorporated the participation of tim and the formation of complexes between PER and TIM proteins revealed that the formation of the PER:TIM complexes favors sustained oscillations by introducing time delays in the negative feedback loop [7]. Qualitatively, this behavior is similar to that observed by a decrease of the Hill coefficient (nH) in core clock models, like the one presented here (S9 Fig. in S1 File). Recent work has highlighted the importance of studying how and to what extent the regulation of PER translation could contribute to those time delays observed experimentally [3]. Our study contributes to this point by showing that the specific kinetic function chosen to represent the translational kinetics would also affect significantly the time delays (Fig. 5 and S7–S17 Figs. in S1 File). Therefore, if the PER translational regulation involves a change in the translation kinetics, then it would also affect the time lags between the per transcript and protein expression profiles. Specifically, we showed that a PER translation taking place with a saturated Michaelis-Menten kinetics can contribute to the pace of the oscillator with longer delays than those produced by a first-order kinetics (Fig. 5), as long as nH = 4, the and the K_r-V_r parameter combination generates oscillations with periods longer than 20 h.

We remark that an increase in the delay follows a decrease in the Hill coefficient, independently of translation kinetics studied in this work (see S7 and S9 Figs. in S1 File). On the other hand, the presence of a Michaelian term in the translation could lead to an increase in the delays only if nH = 4, but not if nH = 1 (see Fig. 5 and S8 Fig. in S1 File). This suggest that the influence of the Hill coefficient on the delay goes beyond that of the translation kinetics. In addition, our results also indicate that the delay-shortening effect derived from an increase in the Hill coefficient can be compensated, at least in part, by changing the function describing the translation kinetics from first order to saturated Michaelis-Menten.

Previous studies showed that the dependence of τ on the PER degradation rate (v_d) can adopt very different profiles depending on the kinetics of other processes, such as the per mRNA and protein degradation, PER phosphorylation-dephosphorylation and nuclear translocation [7, 53]. These results highlight the relevance of the nature of the kinetic functions describing the different biochemical processes on the clock properties. The results shown in Fig. 6 and S18 Fig. in S1 File revealed that the kinetic function describing the PER translational process also affected the dependence of τ on some of the non-translation related parameters of the model. Specifically, we observed that a saturable kinetics of PER translation changed the global sensitivity of the system, decreasing the sensitivity of the period to the synthesis and degradation of per mRNA, but increasing its sensitivity to PER degradation. Therefore, our results provide new information about how the kinetic laws of PER translation can modify the sensitivity of the oscillatory behavior of a core clock model to variations in their parameter values. These results have important implications if we assume that a regulatory process can act on the PER translation by changing its kinetic law. If that were the case, our results revealed that translational regulation may have strong implications for other biochemical process of the clock beyond translation itself. Another possibility would be to assume that the PER translational regulation proceeds without altering the kinetic laws but, instead, by changing the value of the translation-related parameters V_r and K_r. In this case, any alteration of the ‘normal’ regulation of PER translation must be reflected in such parameters. In this respect, the Drosophila TYF protein is a specific factor involved in the translational regulation of PER [25]. Hypomorphic mutations on the tyf gene result in a decreased expression of TYF, reducing the efficiency of PER translation. We have interpreted the effects of the tyf hypomorphic mutation as a decrease in the V_r parameter because such parameter is related to (but not solely determined by) the concentration of specific translational regulators, like TYF. By making this qualitative interpretation of our model, we observed that the period of tyf hypomorphic mutants is shorter than the one observed for WT flies (Fig. 7), which qualitatively agrees with the experimental observations [25]. A similar interpretation of our model can be applied to other recent studies, which reported new proteins involved in the PER translational regulation, such as NAT1 and ATAXIN-2 [26, 48]. Moreover, by interpreting that the tyf hypomorphic mutations will result in a decrease of the TYF levels and, therefore, in a decrease of the V_r parameter value of our model, we have made a qualitative prediction about the time delays expected for the flies carrying such mutations (Fig. 8).

PER translation by two independent mechanisms

Two main mechanisms of translation initiation have been described in Eukaryotes: the canonical cap-dependent and the apparently less frequent cap-independent mechanisms [54]. Transcripts can hypothetically use both mechanisms, but it is unclear to what extent each of these pathways contributes to the total protein expression. Indeed, it is likely that the relative efficiency of one translational mechanism as compared to the other depends on each specific mRNA and the cellular conditions being studied, among other factors [54, 55]. Additionally, recent experimental studies have suggested that PER in Drosophila and PER1 in mammals are translated not only by a cap-dependent mechanism but also by a cap-independent pathway [26, 36, 56]. Because some of the regulators involved in these two translation initiation mechanisms may be different, it is likely that they present different kinetic laws and, therefore, can be modeled as two independent pathways. In this work we studied whether a circadian clock model including a PER translation process consisting of two kinetically different pathways, led to self-sustained oscillations, and how the oscillatory properties of this system were modulated by the relative contribution of those mechanisms. It is worth to emphasize here that our work is the first to systematically study the dynamics of a circadian clock model with an overall translation process derived from two translational mechanisms with different kinetics. Our results showed that it is possible to obtain self-sustained oscillations, with a plethora of periodicities (including the circadian one) and amplitudes that depend on the specific values of the K_r, V_r and k_s parameters. For nH = 4, the V_r-K_r domains, in which self-sustained oscillations exist, became larger as k_s increased, which reflected an increase of the robustness of the system when the R-independent mechanism became more important. A similar behavior was observed when nH = 1, except for k_s = 2, which exhibited a slight decrease in the self-sustained oscillatory domain. Nevertheless, the diversity of observed periods and delays decreased as k_s grew, for both nH = 4 and nH = 1. In addition, for a fixed set of V_r and K_r parameter values, an increase in k_s produced a shortening of the oscillation period, which was revealed by the progressive disappearance of infradian periods in the K_r-V_r diagrams as k_s increased (compare the K_r-V_r diagrams for k_s = 0.16 h⁻¹ and k_s = 2 h⁻¹ in Figs. 3 A).

It is well known that some drugs, like torin or rapamycin, cause inhibition of the canonical cap-dependent translation and shift translation toward a cap-independent activity [26, 28]. However, it is not clear yet whether and to what extent the cap-dependent and cap-independent initiation mechanisms of PER translation are relevant to the maintenance of the circadian period [26, 28]. In this context, mathematical models, like the one proposed here, may be useful to address these issues and to make reasonable predictions. For instance, it was shown that some translational regulators may be involved in the cap-independent translation of PER [28]. Therefore, it could be possible to draw a parallelism between the cap-independent/cap-dependent mechanisms and the R-dependent/R-independent mechanisms in our model, respectively.

This implies that the effect of torin or rapamycin on the cap-dependent translation may be represented in our model by abolishing or reducing the contribution of the R-independent mechanism to the overall translation, achieved by decreasing the value of the k_s parameter. Under these conditions, at least one possible prediction emerging from our work is that treatment with those inhibitors will lengthen the period of the molecular clock (compare the K_r-V_r diagrams for k_s = 0.16 h⁻¹ and k_s = 2 h⁻¹ in Fig. 3 A) and it is expected to be experimentally tested in future works.

Future directions and Concluding Remarks

More refined models of protein production could be also assumed in the future. For instance, an interesting approach could be to consider an oscillatory (circadian) regulation of PER translation, since recent studies in mammals revealed that translation is regulated by the circadian clock both globally (by translation initiation factors) and specifically (by RNA-Binding Proteins, micro RNAs and/or poly(A) tail length regulation) [20, 22, 27, 28].

It is worth noting that deterministic models of the circadian clock (as the one presented here) have been useful for the study of diverse aspects of circadian rhythmicity as long as the number of molecules involved in the oscillatory mechanism exceeds a few tens or hundreds of molecules. However, in some cellular conditions the number of molecules involved in the oscillatory mechanism may be small and deterministic models may reach their limits. In this situation the effect of the intrinsic molecular noise becomes important and it becomes necessary to resort to stochastic models. Previous stochastic models show that circadian oscillations are robust even at low numbers of molecules, although there is a threshold below which the noise obliterates the circadian rhythmicity [9, 57]. In addition, a variety of factors (such as the degree of cooperativity of repression, distance from the bifurcation point, the magnitude of the rate constants characterizing binding of the repressor to the gene, etc.) affects the robustness of the circadian oscillations in the presence of molecular noise. Thus, it would be interesting to explore the implications of assuming a nonlinear saturable kinetics for PER translation in stochastic models of the circadian clock, a problem that will be addressed in future works.

Our results have significant biological relevance because they characterized, for the first time, how different PER translational kinetics affects the generation and maintenance of circadian rhythms at molecular level. We showed that it is possible to modify significantly the period, amplitude, and delays of the self-sustained oscillations by changing the functional form of the PER translation kinetics, by changing the translation-related parameter values, or by considering the PER translation derived from two kinetically different pathways. We have interpreted the effective translational kinetics as the result of the regulation of PER synthesis, which might modify the translational functional term(s) and/or its kinetic parameters. Besides, our model provides results that are in qualitative agreement with experimental findings related to the PER translational regulation of the Drosophila molecular clock and supplies helpful information for interpreting and reassessing the significance of translational regulation in the molecular circadian clock.