Figures
Abstract
Background
We aimed to study whether pulmonary arterial distensibility (PAD) correlates with hemodynamic parameters in chronic thromboembolic pulmonary hypertension (CTEPH) using electrocardiogram (ECG)-gated 320-slice multidetector computed tomography (MDCT).
Methods and Findings
ECG-gated 320-slice MDCT and right heart catheterization (RHC) was performed in 53 subjects (60.6±11.4 years old; 37 females) with CTEPH. We retrospectively measured the minimum and maximum values of the cross sectional area (CSA) of the main pulmonary artery (mainPA), right pulmonary artery (rtPA), and left pulmonary artery (ltPA) during one heartbeat. PAD was calculated using the following formula: PAD = [(CSAmaximum−CSAminimum)/CSAmaximum]×100(%). The correlation between hemodynamic parameters and PAD was assessed. Mean pulmonary arterial pressure (mPAP) and pulmonary vascular resistance (PVR) were 40.8±8.7 mmHg and 8.3±3.0 wood units, respectively. PAD values were as follows: mainPA (14.0±5.0%), rtPA (12.8±5.6%), and ltPA (9.7±4.6%). Good correlations existed between mainPAD, with mPAP (r = −0.594, p<0.001) and PVR (r = −0.659, p<0.001). The correlation coefficients between rtPAD and ltPAD with pulmonary hemodynamics were all lower or equal than for mainPAD.
Citation: Kasai H, Sugiura T, Tanabe N, Sakurai Y, Yahaba M, Matsuura Y, et al. (2014) Electrocardiogram-Gated 320-Slice Multidetector Computed Tomography for the Measurement of Pulmonary Arterial Distensibility in Chronic Thromboembolic Pulmonary Hypertension. PLoS ONE 9(11): e111563. https://doi.org/10.1371/journal.pone.0111563
Editor: Chunxue Bai, Pulmonary Medicine, China
Received: July 3, 2014; Accepted: September 23, 2014; Published: November 3, 2014
Copyright: © 2014 Kasai et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: The authors confirm that all data underlying the findings are fully available without restriction. All relevant data are within the paper.
Funding: The authors have no support or funding to report.
Competing interests: Dr. Tatsumi was supported by a grant to the Respiratory Failure Research Group from the Ministry of Health, Labour and Welfare, Japan (No 21590962). Dr. Tanabe was supported by a research grant from the Ministry of Education, Culture, Sports, Science and Technology of Japan (No 22590849) and belongs to the endowed department sponsored by Actelion Pharmaceuticals, received a grant from the Japanese Respiratory Society, and honoraria from Pfizer. Dr. Shigeta belongs to the endowed department sponsored by Actelion Pharmaceuticals, received a grant from the Japanese Respiratory Society, and honoraria from Pfizer. These do not alter the authors' adherence to PLOS ONE policies on sharing data and materials. Additionally, all data and materials are fully available without restriction.
Introduction
Chronic thromboembolic pulmonary hypertension (CTEPH) can result from either single or recurrent pulmonary embolism arising from deep vein thrombosis [1]. When resistance increases in the pulmonary circulation, the pulmonary artery becomes more distended and less distensible [2]. A study of pulmonary arterial compliance (Cp) suggested that pulmonary arterial distensibility (PAD) decreases with rising pulmonary arterial pressure [3]. PAD is customarily measured using magnetic resonance imaging (MRI) or transthoracic echocardiography, and more recently, computed tomography (CT) [2], [4]–[6]. In addition, although PAD has been correlated with prognosis, exercise tolerance, and pulmonary hemodynamic data among patients with pulmonary hypertension (PH) [6]–[10], the etiology of PH varies, and PAD varies between the main pulmonary artery (mainPA) and the right pulmonary artery (rtPA).
State-of-the-art multidetector CT (MDCT) scanners are now widely available and permit rapid acquisition of a thin-slice dataset, even in breathless patients [11]. A 320-slice MDCT affords 16 cm craniocaudal coverage and volumetric imaging of the entire heart with only a single gantry rotation [12]. In addition, a series of two gantry rotations (double volume scan) in combination with an electrocardiogram (ECG), i.e. ECG-gated 320-slice MDCT, can acquire simultaneous images of the pulmonary arteries and entire heart. Furthermore, because of advances in CT technology, ECG-gated MDCT has been used to image the heart with high spatial and temporal resolution [13].
We hypothesized that ECG-gated 320-slice MDCT could be used for less invasive and simultaneous evaluation of the pulmonary artery and hemodynamic data in the diagnostic assessment of patients with CTEPH. This study therefore aimed to evaluate the relationship between PAD of mainPA, rtPA, and the left pulmonary artery (ltPA) on ECG-gated 320-slice MDCT and hemodynamic data measured by right heart catheterization (RHC).
Materials and Methods
Study population
The study group consisted of consecutive patients with a high clinical suspicion of CTEPH who underwent ECG-gated 320-slice MDCT and RHC from September 2009 to December 2013. The study was approved by the ethics committee of Chiba University (approval number 826). Written informed consent to use their examination results in the future study was obtained from each patient before CT and RHC. Patients with complications including left heart disease, connective tissue disease, severe chronic obstructive pulmonary disease (GOLD Stage III–IV), and severe interstitial pneumonia were excluded.
Enhanced ECG-gated 320-slice CT
All CT scan results were obtained retrospectively from enhanced ECG-gated volume scanning using 320-slice MDCT (Aquilion One, Toshiba Medical, Tochigi, Japan) with a slice thickness of 0.5 mm and 0.35 sec/rotation. To acquire simultaneous images of the pulmonary arteries and the entire heart, an axial series of two gantry rotations in a craniocaudal direction was performed (double volume scan). The resulting dual volume data sets were automatically stitched. Because the most cranial and caudal parts of each volume data set (both 1.6 cm) were not used to create images, the effective scan length was 25.6 cm. The tube voltage and current were set at 120 kV and 580 mA, respectively, with tube current dose modulation. Using a mechanical injector (Dual Shot, Nemoto Tokyo, Japan), 100 mL of contrast media (Iomeron 350 mg/ml, Eisai, Tokyo, Japan) was injected at 3.5 mL/s. Then a saline–contrast mixture was injected: 40 mL of contrast media at 2.0 mL/s, and 30 mL saline at 1.5 mL/s. Time-resolved (per second) single-section CT scans were acquired at the level of the bifurcation of the pulmonary artery without a breath hold. Ascending aortic time-resolved attenuation was then measured using the time-attenuation evaluation program accessible on the scanner. When the CT values in the ascending aorta had increased to 200 Hounsfield units (HU), we began the scan, and the subject was asked to hold the breath.
PAD on ECG-gated 320-slice CT
The CT images were reconstructed at 5% intervals from 0% to 95% of the R–R interval. They were sent to a workstation (Ziostation2; Ziosoft Incorporated) that created cine images of the pulmonary arteries. The cross-sectional area (CSA) of the mainPA (mainPA-CSA) was measured at the one-third position of the pulmonary valve side between the pulmonary valve and the bifurcation of pulmonary artery (Fig. 1A). The CSA of the rtPA (rtPA-CSA) was measured at the central position between the bifurcation of the pulmonary artery and the first branch (Fig. 1B). The CSA of the ltPA (ltPA-CSA) was measured just before the left A3 branch (Fig. 1C). Each CSA position was determined after 30% of the cardiac cycle and adjusted to be perpendicular to the long axis on both axial and coronal views. The minimum and maximum values of each CSA during the cardiac cycle were measured at mediastinal window settings (level, 0 HU; width, 300 HU). When the vessel contained a mural thrombus, we measured the CSA of the blood vessel lumen by using the inner side of the vessel wall as the boundary, ignoring the thrombus. All CSA measurements were performed by two independent readers blinded to the patient’s identity.
(A) CSA of the main pulmonary artery was measured at the one-third position on the pulmonary valve side between the pulmonary valve and the bifurcation of the pulmonary artery. (B) CSA of the right pulmonary artery was measured at the central position between the bifurcation of the pulmonary artery and the first branch. (C) CSA of the left pulmonary artery was measured just before the left A3 branch.
PAD of the mainPA, rtPA, and ltPA (mainPAD, rtPAD, and ltPAD, respectively) were calculated using the following equation, and expressed as percentage variation: PAD = [(CSAmaximum−CSAminimum)/CSAmaximum]×100 (Fig. 2) [14].
(A) 78-year-old woman (mean pulmonary arterial pressure [mPAP], 34 mmHg). The maximum main pulmonary artery (mainPA)-cross sectional area (CSA) was 10.1 cm2, and the minimum was 8.2 cm2. The mainPAD was 18.7%. (B) 61-year-old man (mPAP, 44 mmHg). The maximum mainPA-CSA was 10.6 cm2, and the minimum was 9.6 cm2. The mainPAD was 8.7%.
RHC
A 7.5-French gauge Swan–Ganz thermodilution catheter (Edwards Lifescience, USA) was used via a jugular approach. Pressure measurements were taken from the superior vena cava, right atrium, right ventricle, and mainPA at end-expiration. The zero point was defined as mid-thoracic. Cardiac output (CO) was determined using the thermodilution method by averaging a minimum of three measurements. Left-to-right shunting was ruled out by oximetry. Pulmonary vascular resistance (PVR) in Wood units (WU) was calculated using the equation: PVR = [mean pulmonary artery pressure (mPAP)−pulmonary artery wedge pressure (PAWP)]/CO. Cp was calculated by the ratio between stroke volume (SV) and pulmonary arterial pulse pressure (PAPP).
The PAD measured by enhanced ECG-gated 320-slice MDCT were assessed against the following values obtained by RHC: mPAP, systolic pulmonary arterial pressure (sPAP), diastolic pulmonary arterial pressure (dPAP), PAPP, PVR, CO, cardiac output index (CI), SV, and Cp.
Clinical worsening analysis after sildenafil
The 13 patients who were administered sildenafil were followed for a mean of 24.8±11.1 months (range, 5–39 months). Time to clinical worsening, indicated by death or hospitalization due to right heart failure, between two groups stratified by median value of main PAD (14%) was compared.
Statistical analysis
The Bland–Altman analysis was used to determine the inter- and intra-observer variation in PAD measurements. Pearson’s correlation was used to assess the correlation between hemodynamic data from RHC with PAD obtained using ECG-gated 320-slice MDCT. Correlation analysis of parameters showing a non-normal distribution was carried out using the Kendall tau rank correlation. All results are expressed as the mean ± standard deviation, unless otherwise indicated. A P value<0.05 was considered statistically significant. All statistical analyses were performed using JMP 9.0 software (Cary, North Carolina, USA).
Survival curves were estimated using the Kaplan-Meier method, and the log-rank test was used to compare survival curves according to clinical characteristics.
Results
The study group comprised 53 consecutive patients (age, 60.6±11.4 [SD] years; females, 37) with CTEPH diagnosed by RHC and CT and/or pulmonary angiography. Among them, 24 patients underwent these examinations at the time of the diagnosis, and 29 patients underwent the examinations during follow-up after the diagnosis. Tables 1 and 2 show the baseline characteristics of the patients. The interval range between CT and RHC was two to sixteen days. All patients had perfusion defects (all were larger than segmental), with normal ventilation scans. CTEPH was confirmed by pulmonary angiography in all cases.
PAD
PAD of the mainPA, rtPA, and ltPA showed a trend toward gradual reduction (Table 3); thus, PAD was largest in the mainPA, followed by the rtPA, and then the ltPA, as follows: mainPA (14.0±5.0%), rtPA (12.8±5.6%), and ltPA (9.7±4.6%). The mainPAD and rtPAD correlated with mPAP, sPAP, dPAP, PAPP, PVR, CO, CI, SV; and Cp. The ltPAD correlated with mPAP, sPAP, dPAP, PVR, SV, and Cp. Of these, good correlations existed between mainPAD and both mPAP (r = −0.594) and PVR (r = −0.659) (P<0.001) (Table 4) (Figure 3).
mPAP, mean pulmonary arterial pressure; PVR, pulmonary vascular resistance; MainPAD, main pulmonary arterial distensibility.
Clinical worsening analysis after sildenafil
Time to clinical worsening was slightly longer in the higher main PAD group (≥14%) than in lower mainPAD group (<14%) (log-rank, p = 0.210) (Figure 4). When the rtPAD and ltPAD were used, similar results were observed.
MainPAD, main pulmonary arterial distensibility.
Reproducibility
Absolute intra-observer agreement for mainPAD, rtPAD, and ltPAD was a mean difference of −0.25±0.30%, −0.06±0.20%, and −0.45±0.25%, respectively; the agreement range was 0.36 to −0.84%, 0.34 to −0.46%, and 0.05 to −0.95%, respectively. Absolute inter-observer agreement for mainPAD, rtPAD, and ltPAD was a mean difference of −0.09±0.47%, −0.37±0.48%, and 0.48±0.51%, respectively; the agreement range was 0.86 to −1.05%, 0.60 to −1.32%, and 1.52 to −0.55%, respectively.
Discussion
To the best of our knowledge, this is the first study to assess the ability of 320-slice MDCT to evaluate PAD of each pulmonary artery in subjects with CTEPH. There are two main findings. First, PAD assessed via ECG-gated 320-slice MDCT correlated significantly with mPAP, sPAP, dPAP, PAPP, PVR, CO, CI, SV, and Cp in subjects with CTEPH. Second, the mainPA is suitable for PAD measurements.
Distensibility
The pathophysiological process of CTEPH includes both pulmonary arterial obstruction and the incomplete resolution and organization of pulmonary thromboemboli. However, it also includes vessel remodeling, the release of vasoconstrictive substances, and reflex vasoconstriction over time. This process results in vasculopathy of precapillary vessels similar to that seen in patients with idiopathic pulmonary arterial hypertension [15], [16]. The distensibility of the major arteries is determined by the elastic properties of elastic and collagen fibers, which are the major constituents of the arterial wall [5], [7], [17]–[19]. When the vessel wall is stretched by higher pressure, the less distensible collagen fibers cause increased stiffness [9]. PAD was primarily measured by CT or MRI, and reflected the degree of blood vessel remodeling. In the normally compliant vessel, the range is between 20% and 25% of the minimal diameter, without significant age variation [20]. Previous reports showed that PAD was reduced in subjects with PH compared to normal cases and correlates with prognosis, exercise tolerance, and pulmonary hemodynamic data in subjects with PH [8], [20]. According to a report by Patz et al., normal MRI-based PAD values in 9 healthy individuals were as follows: mainPAD 25.6±10.7%, rtPAD 21.4±10.7%, and ltPAD 24.5±7.8% [21]. The PAD of each pulmonary artery in this study was lower than found in previous reports. Further, PAD decreased in the following order: mainPA, rtPA, and ltPA; the correlation with mPAP and PVR deteriorated in the same order. We also evaluated the correlation of PAD with pulmonary hemodynamics in 42 cases after excluding 11 cases in which the thrombus was deposited at the measurement site (both sides, 3 cases, right side, 8 cases). Some of the correlation coefficients improved for both rtPAD and ltPAD with pulmonary hemodynamics. However, the correlation between the mainPAD and pulmonary hemodynamics remained superior. Thrombi are less likely to adhere to the mainPA; therefore, the mainPA can evaluate the true PAD. Further, the laterality of blood flow and remodeling of blood vessels together with the difference in thrombotic obstruction between rtPA and ltPA could exist in CTEPH. Therefore, the mainPA is suitable for PAD mesurements.
Toshner et al. reported that a relative area change (RAC) in the rtPA which is calculated using the following equation, and expressed as percentage variation: RAC = [(CSAmaximum−CSAminimum)/CSAminimum]×100, correlates with the functional response to sildenafil, the 6-minites walk test (6MWT), N-terminal pro-brain natriuretic peptide (NT pro-BNP), and WHO functional class [22]. In this study, 13 patients were administered sildenafil after measuring the PAD; the PAD was not associated with improvement in either the 6MWT or brain natriuretic peptide (BNP) at a mean of 4.6±1.9 months (range, 2–9 months) after the induction of sildenafil. However, time to clinical worsening, indicated by death or hospitalization due to right heart failure, was slightly longer in the higher mainPAD group than in lower mainPAD group. Further studies are needed to clarify the association of distensibility with response to sildenafil or other drugs. In addition, Mahapatra et al. reported that Cp is a strong predictor of mortality in PAH [23], and Lankhaar et al. reported that Cp strongly contributes to right ventricular afterload [24]. PAD was significantly correlated with Cp in this study. Thus, PAD may be a prognostic indicator.
MDCT has advantages over MRI for PAD evaluation. Using ECG-gated 320-slice MDCT, all cross-sections in the pulmonary artery cine images can be observed, even after imaging. Using MRI, it is necessary to select a cross-section to view before imaging; this requirement presents a limitation, as the pulmonary artery deforms easily and moves during the cardiac cycle. In addition, the breath-hold time was no more than 10 s with MDCT, which would be beneficial in patients with CTEPH who may be unable to perform the extended breath hold required for MRI [13]. Thus, CT could be superior to MRI for pulmonary artery evaluation in patients with CTEPH. However, caution must be exercised in choosing CT due to radiation exposure, even though the radiation burden with 320-slice volume CT is lower than that with helical CT, as it avoids overlapping rotations [13]. In the present study, the total radiation dose was approximately 10−20 millisieverts. In addition, CT also allowed us to evaluate coronary artery disease and thus assess the necessity of simultaneous coronary artery graft bypass surgery during pulmonary endarterectomy as a substitute for conventional invasive coronary angiography [12], [25]. Such benefits must be weighed against the potential risk from radiation exposure.
Study limitations
This study had some limitations. First, this single-center retrospective study included a small number of subjects. However, because the disease is rare, the present report could be noteworthy within this field. Second, the correlation between enhanced ECG-gated 320-slice MDCT parameters and RHC pulmonary hemodynamic data should ideally be studied by performing the two examinations on the same day; in our study, most patients underwent the two examinations within 16 days of each other.
Author Contributions
Conceived and designed the experiments: HK TS NT. Performed the experiments: HK TS NT SS YK. Analyzed the data: HK TS NT YS YM MY AS NK. Contributed reagents/materials/analysis tools: HK TS. Contributed to the writing of the manuscript: HK TS NT KT.
References
- 1. Fedullo P, Kerr KM, Kim NH, Auger WR (2011) Chronic thromboembolic pulmonary hypertension. Am J Respir Crit Care Med 183: 1605–1613.
- 2. Bogren HG, Klipstein RH, Mohiaddin RH, Firmin DN, Underwood SR, et al. (1989) Pulmonary artery distensibility and blood flow patterns: a magnetic resonance study of normal subjects and of patients with pulmonary arterial hypertension. Am Heart J 118: 990–999.
- 3. Reuben SR (1971) Compliance of the human pulmonary arterial system in disease. Circ Res 29: 40–50.
- 4. Bouchard A, Higgins CB, Byrd BF 3rd, Amparo EG, Osaki L, et al. (1985) Magnetic resonance imaging in pulmonary arterial hypertension. Am J Cardiol 56: 938–942.
- 5. Pasierski TJ, Starling RC, Binkley PF, Pearson AC (1993) Echocardiographic evaluation of pulmonary artery distensibility. Chest 103: 1080–1083.
- 6. Revel MP, Faivre JB, Remy-Jardin M, Delannoy-Deken V, Duhamel A, et al. (2009) Pulmonary hypertension: ECG-gated 64-section CT angiographic evaluation of new functional parameters as diagnostic criteria. Radiology 250: 558–566.
- 7. Harris P, Heath D, Apostolopoulos A (1965) Extensibility of the pulmonary trunk in heart disease. Br Heart J 27: 660–666.
- 8.
Gan CT, Lankhaar JW, Westerhof N, Marcus JT, Becker A, et al.. (2007) Noninvasively assessed pulmonary artery stiffness predicts mortality in pulmonary arterial hypertension. Chest. 1906–1912.
- 9. Wu DK, Hsiao SH, Lin SK, Lee CY, Yang SH, et al. (2008) Main pulmonary arterial distensibility: different presentation between chronic pulmonary hypertension and acute pulmonary embolism. Circ J 72: 1454–1459.
- 10. Kang KW, Chang HJ, Kim YJ, Choi BW, Lee HS, et al. (2011) Cardiac magnetic resonance imaging-derived pulmonary artery distensibility index correlates with pulmonary artery stiffness and predicts functional capacity in patients with pulmonary arterial hypertension. Circ J 75: 2244–2251.
- 11. Jenkins D, Mayer E, Screaton N, Madani M (2012) State-of-the-art chronic thromboembolic pulmonary hypertension diagnosis and management. Eur Respir Rev 21: 32–39.
- 12. Dewey M, Zimmermann E, Deissenrieder F, Laule M, Dubel HP, et al. (2009) Noninvasive coronary angiography by 320-row computed tomography with lower radiation exposure and maintained diagnostic accuracy: comparison of results with cardiac catheterization in a head-to-head pilot investigation. Circulation 120: 867–875.
- 13. Sugiura T, Tanabe N, Matsuura Y, Shigeta A, Kawata N, et al. (2013) Role of 320-slice ct imaging in the diagnostic workup of patients with chronic thromboembolic pulmonary hypertension. Chest 143: 1070–1077.
- 14. Jardim C, Rochitte CE, Humbert M, Rubenfeld G, Jasinowodolinski D, et al. (2007) Pulmonary artery distensibility in pulmonary arterial hypertension: an MRI pilot study. Eur Respir J 29: 476–481.
- 15. Dartevelle P, Fadel E, Mussot S, Chapelier A, Herve P, et al. (2004) Chronic thromboembolic pulmonary hypertension. Eur Respir J 23: 637–648.
- 16. Sacks RS, Remillard CV, Agange N, Auger WR, Thistlethwaite PA, et al. (2006) Molecular biology of chronic thromboembolic pulmonary hypertension. Semin Thorac Cardiovasc Surg 18: 265–276.
- 17. Roach MR, Burton AC (1957) The reason for the shape of the distensibility curves of arteries. Can J Biochem Physiol 35: 681–690.
- 18. Slife DM, Latham RD, Sipkema P, Westerhof N (1990) Pulmonary arterial compliance at rest and exercise in normal humans. Am J Physiol 258: H1823–1828.
- 19. Gorgulu S, Eren M, Yildirim A, Ozer O, Uslu N, et al. (2003) A new echocardiographic approach in assessing pulmonary vascular bed in patients with congenital heart disease: pulmonary artery stiffness. Anadolu Kardiyol Derg 3: 92–97.
- 20. Botney MD (1999) Role of hemodynamics in pulmonary vascular remodeling: implications for primary pulmonary hypertension. Am J Respir Crit Care Med 159: 361–364.
- 21. Paz R, Mohiaddin RH, Longmore DB (1993) Magnetic resonance assessment of the pulmonary arterial trunk anatomy, flow, pulsatility and distensibility. Eur Heart J 14: 1524–1530.
- 22. Toshner MR, Gopalan D, Suntharalingam J, Treacy C, Soon E, et al. (2010) Pulmonary arterial size and response to sildenafil in chronic thromboembolic pulmonary hypertension. J Heart Lung Transplant 29: 610–615.
- 23. Mahapatra S, Nishimura RA, Sorajja P, Cha S, McGoon MD (2006) Relationship of pulmonary arterial capacitance and mortality in idiopathic pulmonary arterial hypertension. J Am Coll Cardiol 47: 799–803.
- 24. Lankhaar JW, Westerhof N, Faes TJ, Gan CT, Marques KM, et al. (2008) Pulmonary vascular resistance and compliance stay inversely related during treatment of pulmonary hypertension. Eur Heart J 29: 1688–1695.
- 25. Kim NH (2006) Assessment of operability in chronic thromboembolic pulmonary hypertension. Proc Am Thorac Soc 3: 584–588.