Correction
20 Nov 2014: The PLOS ONE Staff (2014) Correction: Dietary Protein Intake and Coronary Heart Disease in a Large Community Based Cohort: Results from the Atherosclerosis Risk in Communities (ARIC) Study. PLOS ONE 9(11): e114114. https://doi.org/10.1371/journal.pone.0114114 View correction
Figures
Abstract
Background
Prospective data examining the relationship between dietary protein intake and incident coronary heart disease (CHD) are inconclusive. Most evidence is derived from homogenous populations such as health professionals. Large community-based analyses in more diverse samples are lacking.
Methods
We studied the association of protein type and major dietary protein sources and risk for incident CHD in 12,066 middle-aged adults (aged 45–64 at baseline, 1987–1989) from four U.S. communities enrolled in the Atherosclerosis Risk in Communities (ARIC) Study who were free of diabetes mellitus and cardiovascular disease at baseline. Dietary protein intake was assessed at baseline and after 6 years of follow-up by food frequency questionnaire. Our primary outcome was adjudicated coronary heart disease events or deaths with following up through December 31, 2010. Cox proportional hazard models with multivariable adjustment were used for statistical analyses.
Results
During a median follow-up of 22 years, there were 1,147 CHD events. In multivariable analyses total, animal and vegetable protein were not associated with an increased risk for CHD before or after adjustment. In food group analyses of major dietary protein sources, protein intake from red and processed meat, dairy products, fish, nuts, eggs, and legumes were not significantly associated with CHD risk. The hazard ratios [with 95% confidence intervals] for risk of CHD across quintiles of protein from poultry were 1.00 [ref], 0.83 [0.70–0.99], 0.93 [0.75–1.15], 0.88 [0.73–1.06], 0.79 [0.64–0.98], P for trend = 0.16). Replacement analyses evaluating the association of substituting one source of dietary protein for another or of decreasing protein intake at the expense of carbohydrates or total fats did not show any statistically significant association with CHD risk.
Citation: Haring B, Gronroos N, Nettleton JA, Wyler von Ballmoos MC, Selvin E, Alonso A (2014) Dietary Protein Intake and Coronary Heart Disease in a Large Community Based Cohort: Results from the Atherosclerosis Risk in Communities (ARIC) Study. PLoS ONE 9(10): e109552. https://doi.org/10.1371/journal.pone.0109552
Editor: Antony Bayer, Cardiff University, United Kingdom
Received: May 7, 2014; Accepted: September 4, 2014; Published: October 10, 2014
Copyright: © 2014 Haring et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: The authors confirm that all data underlying the findings are fully available without restriction. All data underlying the findings in our study are freely available in a public repository governed by the ‘Biologic Specimen and Data Repository Information Coordinating Center (BioLINCC)’ under the auspices of the National Heart, Lung and Blood Institute (NHLBI). The repository can be accessed online (https://biolincc.nhlbi.nih.gov/studies/aric/?q=aric).
Funding: The Atherosclerosis Risk in Communities Study is carried out as a collaborative study supported by National Heart, Lung, and Blood Institute contracts (HHSN268201100005C, HHSN268201100006C, HHSN268201100007C, HHSN268201100008C, HHSN268201100009C, HHSN268201100010C, HHSN268201100011C, and HHSN268201100012C). There are no relationships with industry to declare. Open Access publication was funded by the German Research Foundation (DFG) and the University of Würzburg in the funding program Open Access Publishing. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Introduction
The relationship of dietary protein distinguished by animal versus vegetable origin with risk of coronary heart disease (CHD) has shown conflicting results [1], [2], [3], [4], [5], [6]. This is surprising since the type of protein has been shown to influence cardiovascular risk factors such as hypertension [7], [8], [9], [10], [11], [12]. Various observational studies [7], [8], [10], [13] and feeding trials [9], [11], [12] have associated dietary protein of vegetable type inversely with blood pressure. To elucidate this apparent paradox, Bernstein et al. have focused on the effect of various food groups as major sources of dietary protein rather than on protein type in the Nurses' Health Study [2]. Greater consumption of red meat or processed meat products was associated with a higher risk of CHD, while higher intakes of poultry, fish and nuts were associated with lower risk [2]. These findings are again in contrast with other results that showed no association of red meat with CHD [14] or that showed beneficial effects of animal protein on vascular health [15], [16]. The discordance in findings may be explained by several factors including research design and study populations. Large randomized controlled feeding trials on this topic are sparse and current evidence is mostly derived from observational studies which used nurses or health professionals as study populations [1], [2], [4], [5]. Community based analyses are mostly missing [6], [17]. Thus, conclusions regarding the relation of various sources of protein intake with cardiovascular health are difficult to draw. Analyses conducted in large general communities are warranted as these may provide greater exposure variability with more generalizable results.
In this study, we aimed to investigate the associations between total, animal, and plant-based dietary protein, as well as individual protein-rich food groups, and the risk for CHD in a large, community-based cohort of middle-aged adults. We hypothesized that intake of animal protein and proteins from processed meats would be associated with a higher risk of CHD and vegetable proteins and corresponding food groups with a lower CHD risk.
Methods
Study Population
The Atherosclerosis Risk in Communities Study (ARIC) is a community-based prospective cohort study of 15,792 middle-aged adults (aged 45–64 years at baseline) from four U.S. communities (Washington County, Md; Forsyth County, NC; Jackson, Miss; and suburbs of Minneapolis, Minn.) [18]. The first examination (visit 1) of participants occurred during 1987–1989, with three follow-up visits taking place each approximately every 3 years; response rates were 93%, 86%, and 81% at visits 2 (1990 to 1992), 3 (1993 to 1995), and 4 (1996 to 1998), respectively. A fifth exam (visit 5) took place in 2011–2013 among surviving participants. At all visits, participants received an extensive examination, including collection of medical, social, and demographic data [18]. For this analysis, only white and black adults were included; blacks from the Minneapolis and Washington County field centers were excluded due to small numbers. Individuals with self-reported diabetes, fasting blood glucose ≥126 mg/dL, non-fasting blood glucose ≥200 mg/dL or use of diabetes medication, a history of myocardial infarction, stroke, heart failure, coronary bypass surgery, angioplasty or with missing data on covariates of interest were excluded. Our final sample size included 12,066 persons.
The ARIC study was approved by the Institutional Review Boards (IRB) of all participating institutions, including the IRBs of the University of Minnesota, Johns Hopkins University, University of North Carolina, University of Mississippi Medical Center, and Wake Forest University. Written informed consent at each clinical site was obtained from all participants.
Assessment of protein intake
Protein intake was assessed using an interviewer-administered, 66-item food frequency questionnaire (FFQ) adapted from the 61-item FFQ developed by Willett et al. [19]. The FFQ was administered to all subjects at visit 1 at baseline (1987–1989) and at visit 3 (1993–1995). The usual frequency of food consumption was reported in 9 categories, from never or less than once a month to>6 times per day. The major contributors to protein intake included: unprocessed red meat, processed red meat, poultry, high-fat dairy, low-fat dairy, fish & seafood, eggs, nuts, and legumes. Average daily intake of nutrients was calculated by multiplying the frequency of consumption of each food item by its nutrient content and adding up the nutrient intake for all of the items. Vegetable protein intake was defined as the difference of total and animal protein intake. The residual method was used to adjust for total energy intake [20]. For assessing dietary behaviour, participants were divided into quintiles of cumulative average intake of various protein sources. Cumulative updating of the FFQ (i.e. visit 1 FFQ for follow-up between visit 1 and visit 3 and the average of visits 1 and 3 FFQ afterwards for those who attended both examinations, or visit 1 FFQ for those who did not attend visit 3) was used to reduce within-person variation and best represent long-term dietary behavior [2]. Participants with incomplete dietary information or with extreme calorie intake (<600 kcal or>4200 kcal per day for men, <500 kcal or>3600 kcal per day for women) were excluded from further analysis. We stopped updating a participant's cumulative average intake when the participant of our study was diagnosed with an intermediate variable on the causal pathway between diet and CHD such as hypercholesterolemia, hypertension, stroke and diabetes. This was done to avoid exposure misclassification due to short-term changes in dietary patterns.
Assessment of coronary heart disease
The primary end point for this study was CHD occurring after the completion of the first FFQ (between 1987 and 1989). CHD was defined as a definite or probable myocardial infarction or a death from coronary heart disease. CHD events were identified and adjudicated using information from study visits, yearly telephone follow-up calls, review of hospital discharge lists and medical charts, death certificates, next-of-kin interviews, and physician-completed questionnaires [18], [21]. Follow-up for CHD was available until December 31, 2010.
Covariates
Height, weight, and waist circumference were measured following a standardized protocol [18], [21]. ARIC participants underwent fasting venipuncture at each examination [18]. Diabetes was defined as current use of glucose-lowering medications, fasting blood glucose ≥126 mg/dL, non-fasting blood glucose ≥200 mg/dL or self-reported history of diabetes. Hypertension was defined as the average of the last two of three blood-pressure readings at the first visit (using 140 mmHg or higher for systolic and 90 mmHg or higher for diastolic as cut-off points). Current smoking, ethanol intake, education, intake of antihypertensive or lipid lowering medication were derived from standardized questionnaires [18]. Sports-related physical activity and leisure related physical activity were assessed with the use of Baecke's questionnaire and scoring systems [22].
Statistical Analysis
To assess the association of CHD and average cumulative intake of protein by quintiles, we calculated incidence rates (IR) of CHD events per 1000 person-years as the number of diagnosed cases of CHD occurring during the entire follow-up period divided by person-years of follow-up. Person-years of follow up were defined as time from the baseline examination to the date of the first coronary event, death, lost to follow-up, or December 31, 2010, whichever occurred earlier. Thereafter, corresponding rate ratios were calculated by dividing the rate among participants in each specific intake quintile by the rate among participants in the lowest quintile of intake (reference). Cox proportional hazards regression models were used to account for potential confounding. An initial model adjusted for age, race, sex, ARIC study center, and total energy intake (minimally adjusted model). A second model additionally adjusted for smoking (current, former, never), pack years of smoking, education (less than high school, high school, more than high school), systolic blood pressure (mmHg), use of antihypertensive medication, HDLc (mmol/l), total cholesterol (mmol/l), use of lipid lowering medication, body mass index (kg/m2), waist-to-hip ratio, alcohol intake (g/week), Baecke's physical activity score, leisure-related physical activity, carbohydrate intake (quintiles), fiber intake (quintiles), and magnesium intake (quintiles) (fully adjusted model). Median protein intake of each quintile (g/d) modeled as a continuous variable was used to test for linear trend.
We further conducted food substitution analyses based on the fully adjusted model. Hazard ratios of CHD associated with increasing 1 serving/day in the consumption of protein sources at the expense of decreasing 1 serving/day in a different protein source were calculated. Similarly, we conducted nutrient substitution analyses by examining the risk for CHD when increasing 10% energy from carbohydrates or fat while decreasing 10% of energy from protein. Tests of the proportional hazards assumption were evaluated. All p-values were 2-tailed. Data were analyzed with SAS 9.3 (SAS Corp, Cary, NC).
Results
Baseline characteristics of the study participants according to quintiles of total protein intake at baseline are shown in Table 1. Compared with participants with low protein consumption, individuals with high protein consumption were less likely to be current smoker, to drink less alcohol per week, and more likely to conduct physical activity and to have graduated from high school. Furthermore, participants with high protein intake had higher BMI levels, higher intakes of fiber, magnesium and fat whereas decreased intake of carbohydrates.
During a median follow-up of 22 years, there were 1,147 CAD events among the 12,066 participants at baseline. In age, sex, race, study center and total energy adjusted analyses (minimally adjusted model) animal protein intake was not associated with an increased risk for CHD (Table 2). These results did not change significantly after full adjustment. In the minimally adjusted model, total and vegetable protein were associated with a significantly lower risk of CHD (Table 2, Model 1). This relationship was considerably attenuated and became non-significant after full adjustment (Table 2, Model 2).
In food-group analyses of major dietary protein sources using our minimally adjusted model, higher intake of red or processed meat was significantly associated with increased risk for CHD, whereas low-fat dairy, poultry and nuts consumption were significantly associated with decreased risk for CHD (Table 3, Model 1). After adjustment for potential confounders, only higher poultry intake remained associated with a lower risk of CHD (Table 3, Model 2).
Last, we conducted replacement analysis evaluating the association of substituting one source of dietary protein for another and of decreasing protein intake at the expense of carbohydrates or total fats (Table 4). Overall, these results did not show any statistically significant association with CHD risk, although they suggested that decreasing red meat and increasing intake of any other protein source was associated with non-significant lower risk of CHD (10–20% reduction per 1 serving/day change).
Discussion
In this prospective community based study with 22 years of follow-up, neither total, animal or vegetable protein intake was associated with risk of CHD. In food group analyses of major dietary protein sources, we found no significant trend between various sizes of intake of meat products, poultry, dairy, eggs, nuts, fish, or legumes and risk for CHD. Our results are contrary to our initial hypothesis as we expected food groups based on animal protein such as red or processed meat products to be significantly associated with an increased risk for CHD.
Thus far, the largest cohort analyses to examine an association between protein intake and coronary heart disease were undertaken using data from the Nurses' Health Study with 14, 16 and 26 years of follow up [2], [5], [23]. Interestingly, similar to our results it was found that after 14 to 16 years of follow-up, neither animal protein nor vegetable protein were associated with CHD [5], [24]. Results from the Health Professional Follow-up Study also indicate no association between dietary protein and risk of coronary heart disease after 18 years of follow-up [4]. In a later analysis of the Nurses' Health Study spanning 26 years of follow-up higher intakes of red meat, red meat excluding processed meat, and high-fat dairy were indeed found to be significantly associated with an elevated risk of CHD while higher intakes of poultry, fish, and nuts were significantly associated with lower risk [2]. Other prospective studies using California Seventh Day Adventists or the NIH-AARP Diet and Health cohort as study base also report a positive association between (red) meat consumption and CHD risk [6], [25]. Nonetheless, generalizability of the existing data is limited as the respective cohorts are characterized by well-educated, ethnically homogenous study populations. A recent meta-analysis summarizing 9 studies on red and processed meat consumption and risk for CHD found processed meats (RR 1.42, 95%CI 1.07, 1.89), but not red meats (RR 1.00, 95% CI 0.81,1.23) to increase incident coronary events [14]. The effects of other dietary protein sources or type of protein were not addressed in this analysis. Moreover all included studies were observational and among included studies only one was based in a general community setting in the UK [17]. Interventional studies such as the Bold Study suggest that dietary protein, also of animal origin, can exert positive effects on biomarkers of CHD [15], [16]. Lean beef in an optimal lean diet has been shown to exhibit beneficial effects on systolic blood pressure and vascular elasticity [15], [16].
In spite of the lack of strong epidemiologic evidence for an association between animal derived protein sources (in particular meat products) and risk for CHD, several arguments mainly based on contents of sodium and saturated fat have been previously made to potentially explain a harmful effect of animal derived protein products on the risk of CHD. Processed meats are known to have high sodium contents. High sodium intake is strongly correlated with the development of hypertension and CHD [26], [27]. On the other hand, high animal protein as provided by dark meat intake often accompanies large intakes of saturated fat intake which has been linked to increased cardiovascular risk [23], [28], [29]. In contrast to animal protein diets consisting of vegetable protein have been associated with cardiovascular and overall health benefits because of their high content of mono- and polyunsaturated fats, fiber, vitamins and minerals and low content of sodium [30]. These lines of argumentation are supported by current dietary recommendations [31], however, the role of saturated fat on the risk of CHD has been subject to controversial debates most recently [32], [33]. Further research is warranted to elucidate the mechanisms of action of protein on the risk of CHD.
The absence of an association between major dietary protein sources and risk for CHD in our population may be explained in part by limited variation in consumptions of these food groups. Our study participants reported low meat intake whereas consumption of eggs, nuts, fish and dairy consumption were similar to other study populations [2]. Therefore, our observation suggesting a protective association of high poultry intake with lower CHD risk has to be interpreted with caution as this singular result may be spurious.
Other reasons that explain inconsistent findings and reports between dietary protein sources, food groups and CHD can be found in differences in study design, follow-up and assessment of outcomes and covariates. Similar to previous reports of the Nurses' Health Study and the Health Professional Study a follow-up period of 22 years may not have been long enough to detect significant differences between various dietary intake levels and risk for CHD events [2], [4], [5]. Second, our dietary data assessment was imperfect and incomplete. Although repeated dietary data measurements may take changing dietary patterns into account and thus serve to reduce intra-individual error, exposure variability of our study population was limited as protein intake was only assessed at two time-points. Changing dietary habits may not have been covered adequately by our FFQs with time-points only 6 years apart. On the other hand, it is well known that behavioral dietary changes are very challenging to accomplish and to maintain on the individual level and thus great changes in the overall population are unlikely to occur [34], [35]. Last, dietary substitution effects as well as different characteristics of particular food group (e.g fat content, micronutrient content) may limit our analyses. Strengths of our study include the sample size, a large community based cohort with two different races in the setting of the general US population and a prospective design with long follow-up. CHD and several confounding factors were assessed using standardized protocols whereas other studies were based on self-report data [2], [5].
In conclusion, using a large community based cohort study we found neither total nor animal or vegetable protein to be associated with CHD. In detailed food group analyses of major protein sources, no statistically significant trends between animal or vegetable-based food groups and risk for CHD were observed. Individuals should continue to make appropriate dietary modifications following current guidelines and recommendations for cardiovascular disease risk reduction [16], [36].
Acknowledgments
The authors thank the staff and participants of the ARIC study for their important contributions. AA, BH and NG had full access to all of the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis.
Author Contributions
Conceived and designed the experiments: BH NG JN MWvB ES AA. Performed the experiments: ES JN BH AA. Analyzed the data: AA NG BH. Contributed reagents/materials/analysis tools: JN NG ES BH MWvB AA. Wrote the paper: BH NG JN MWvB ES AA.
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