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Correction

Correction: Establishment of a Novel Fluorescence-Based Method to Evaluate Chaperone-Mediated Autophagy in a Single Neuron

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Figure S4 is incorrect. The second image in the first column of Figure S4C is from the incorrect cell. Please view the correct Figure S4 below.

Supporting Information

Figure S4.

Immunostaining of LC3 and LAMP2A in HeLa cells displaying GAPDH-HT dots in the presence or absence of CMA activators. (A) Representative GAPDH-HT fluorescence (upper panels), LC3 immunostaining (center panels) and merged (lower panels) images of HeLa cells treated with vehicle (0.1% DMSO, 0.1% methanol), serum free medium (0.1% DMSO, 0.1% methanol), H2O2 (100 µM) or MPA (10 µM) 21 h after labeling with TMR-HT ligand. While LC3-positive dots that represent autophagosomes were distributed diffusely in the cytoplasm, GAPDH-HT dots accumulated in the perinuclear region in the absence or presence of CMA activators. Bar  =  5 µm. (B) Higher magnification images of squares in merged images of serum (−) (upper) and H2O2 (lower) treatments. Although serum deprivation and H2O2 increased the number of LC3-positive dots, these dots rarely colocalized with GAPDH-HT dots, suggesting that GAPDH-HT dots do not result from macroautophagy. (C) Representative GAPDH-HT fluorescence (upper panels), LC3 immunostaining (center panels) and merged (lower panels) images of HeLa cells treated with vehicle, serum free medium, H2O2 or MPA 21 h after labeling with TMR-HT ligand. Bar  =  5 µm. (D) Higher magnification images of squares in merged images of serum (−) (upper) and H2O2 (lower) treatments. GAPDH-HT dots colocalized with or were surrounded by LAMP2A-positive dots in the absence or presence of CMA activators, indicating that lysosomal translocation of GAPDH-HT is mediated by CMA.(TIF)

https://doi.org/10.1371/journal.pone.0109006.s001

(TIF)

Reference

  1. 1. Seki T, Yoshino K-i, Tanaka S, Dohi E, Onji T, et al. (2012) Establishment of a Novel Fluorescence-Based Method to Evaluate Chaperone-Mediated Autophagy in a Single Neuron. PLoS ONE 7(2): e31232

Citation: The PLOS ONE Staff (2014) Correction: Establishment of a Novel Fluorescence-Based Method to Evaluate Chaperone-Mediated Autophagy in a Single Neuron. PLoS ONE 9(9): e109006. https://doi.org/10.1371/journal.pone.0109006

Published: September 17, 2014

Copyright: © 2014 The PLOS ONE Staff. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.