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Correction

Correction: Co-Expression of Bacterial Aspartate Kinase and Adenylylsulfate Reductase Genes Substantially Increases Sulfur Amino Acid Levels in Transgenic Alfalfa (Medicago sativa L.)

  • The PLOS ONE Staff
  • Published: August 01, 2014
  • DOI: 10.1371/journal.pone.0105182

Figure S1A is incorrect. Please see the corrected Figure S1 below.

Supporting Information

Figure S1.

Molecular analysis of T1 wild-type and transgenic plants. A. PCR analysis of AK and APR genes in T1 transgenic alfalfa plants. Lane WT: wild-type line; Lane1-8: T1-BD1-8 transgenic alfalfa lines. +: positive control(vector). B. AK and APR relative expression levels of T1 transgenic alfalfa plants in RT-qPCR analysis. Lane WT: wild-type line; Lane T1-BD1,5,8: T1 transgenic alfalfa lines. Each bar represents the mean of three biological replicates±SE. ** represents statistically significant differences (P<0.01). C. Western blot assay of expression of APR protein in T1 transgenic alfalfa lines. Lane WT: wild-type line; Lane T1-BD1,5,8: T1 transgenic alfalfa lines; +: 6×His-APR fused protein; Lane M: PageRular™ prestained protein ladder (Thermo scientific,USA). 26 kDa and 34 kDa indicate the standard marker bands.

doi:10.1371/journal.pone.0105182.s001

(TIF)

Reference

  1. 1. Tong Z, Xie C, Ma L, Liu L, Jin Y, et al. (2014) Co-Expression of Bacterial Aspartate Kinase and Adenylylsulfate Reductase Genes Substantially Increases Sulfur Amino Acid Levels in Transgenic Alfalfa (Medicago sativa L.). PLoS ONE 9(2): e88310 doi:10.1371/journal.pone.0088310.