Figures
There is an error in Figure 2. The arrow representing activation term IV is incorrectly directed towards the PGK enzyme; it should be directed towards the PK enzyme. Please see the correct Figure 2 here.
Symbol “↓” indicates activation and “⊥” inhibition. Glycolytic enzymes are either inhibited or activated
by an effector “α”. The corresponding activation/inhibition terms are labeled as I, II, III, IV, and V. The bottom diagram represents model simulations for parental, induced low- and induced high-producer cell lines with no regulation (solid black line), with the addition of term I (solid red line), with the addition of terms I and II (solid blue line), and the addition of all terms (solid green line). Experimental data are represented by triangles (parental culture), squares (induced low-producing culture), and circles (induced high-producing culture) for cell density (A), glucose (B), ATP-to-ADP ratio (C), and NADH-to-NAD ratio (D). Error bars are standard deviations from duplicate flasks. Error bars are standard deviations for duplicate cultures.
Reference
Citation: The PLOS ONE Staff (2014) Correction: Analyzing Clonal Variation of Monoclonal Antibody-Producing CHO Cell Lines Using an In Silico Metabolomic Platform. PLoS ONE 9(8): e104725. https://doi.org/10.1371/journal.pone.0104725
Published: August 1, 2014
Copyright: © 2014 The PLOS ONE Staff. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.