Figure 4. Heterogeneity of hbl expression in planktonic cultures and in biofilms.
A: Flow cytometry analysis of bacteria expressing Phbl'-yfp in planktonic cultures or in biofilms, shown as histogram plot. The blue-filled curve shows biofilm data, the yellow-filled curve shows planktonic cultures data and the unfilled dashed curve shows data from bacteria lacking yfp. B: Flow cytometry analysis of bacteria expressing Papha3'-yfp in biofilms (blue-filled curve) compared to bacteria lacking yfp (unfilled dashed curve), shown as histogram plot. C: Expression from the hbl promoter was monitored in planktonic cultures and in biofilms by epifluorescence microscopy through a transcriptional fusion to yfp. Cell limits are shown by the membrane stain FM4-64 (red).doi:10.1371/journal.pone.0087532.g004
Figure 5. Expression of hbl and of sinI in biofilms.
A: Flow cytometry analysis of bacteria expressing Phbl'-yfp and PsinI'-mcherry in 48 h-old biofilms, shown as dot-plot. While 72% of the bacteria do not express hbl nor sinI (quadrant d), 15% of the cells which express hbl also express sinI (quadrant b), and 12% of the bacteria express sinI but nothbl (quadrant a). B: Observation by epifluorescence microscopy of bacteria expressing Phbl'-yfp (left, in green) and PsinI'-mcherry (center, in red) in 48 h-old biofilms. An overlay of YFP fluorescence (hbl expression), mCherry fluorescence (sinI expression) and phase contrast microscopy is shown on the right.doi:10.1371/journal.pone.0087532.g005
Citation: The PLOS ONE Staff (2014) Correction: SinR Controls Enterotoxin Expression in Bacillus thuringiensis Biofilms. PLoS ONE 9(4): e96707. doi:10.1371/journal.pone.0096707
Published: April 25, 2014
Copyright: © 2014 The PLOS ONE Staff. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.