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Variable Virulence Factors in Burkholderia pseudomallei (Melioidosis) Associated with Human Disease

  • Derek S. Sarovich ,

    Contributed equally to this work with: Derek S. Sarovich, Erin P. Price

    derek.sarovich@menzies.edu.au

    Affiliation Global and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia

  • Erin P. Price ,

    Contributed equally to this work with: Derek S. Sarovich, Erin P. Price

    Affiliation Global and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia

  • Jessica R. Webb,

    Affiliation Global and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia

  • Linda M. Ward,

    Affiliation Global and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia

  • Marcos Y. Voutsinos,

    Affiliation Global and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia

  • Apichai Tuanyok,

    Affiliation Department of Tropical Medicine, Medical Microbiology and Pharmacology, University of Hawai’i, Honolulu, Hawai’i, United States of America

  • Mark Mayo,

    Affiliation Global and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia

  • Mirjam Kaestli,

    Affiliation Global and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia

  • Bart J. Currie

    Affiliations Global and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia, Infectious Diseases Department, Royal Darwin Hospital, Darwin, Northern Territory, Australia

Abstract

Burkholderia pseudomallei is a Gram-negative environmental bacterium that causes melioidosis, a potentially life-threatening infectious disease affecting mammals, including humans. Melioidosis symptoms are both protean and diverse, ranging from mild, localized skin infections to more severe and often fatal presentations including pneumonia, septic shock with multiple internal abscesses and occasionally neurological involvement. Several ubiquitous virulence determinants in B. pseudomallei have already been discovered. However, the molecular basis for differential pathogenesis has, until now, remained elusive. Using clinical data from 556 Australian melioidosis cases spanning more than 20 years, we identified a Burkholderia mallei-like actin polymerization bimABm gene that is strongly associated with neurological disease. We also report that a filamentous hemagglutinin gene, fhaB3, is associated with positive blood cultures but is negatively correlated with localized skin lesions without sepsis. We show, for the first time, that variably present virulence factors play an important role in the pathogenesis of melioidosis. Collectively, our study provides a framework for assessing other non-ubiquitous bacterial virulence factors and their association with disease, such as candidate loci identified from large-scale microbial genome-wide association studies.

Introduction

Burkholderia pseudomallei and its equine-adapted clonal derivative Burkholderia mallei (the causal agent of glanders) are formidable pathogens. In 2012, B. pseudomallei and B. mallei were upgraded to Tier 1 Select Agent status in the United States, a ranking reserved for only those infectious agents that pose the greatest risk to human and animal health and which also includes Bacillus anthracis (anthrax) and Yersinia pestis (plague) [1]. Our knowledge of the global distribution of B. pseudomallei is expanding as regions of endemicity continue to be unveiled, with this bacterium now considered endemic in tropical northern Australia, many countries in Asia, selected locations in the Americas and Africa, and several islands in the Indian and Pacific Ocean islands [2]. Northeast Thailand and the tropical Top End of the Northern Territory in Australia are hyperendemic for this bacterium, with melioidosis being a major cause of community-acquired pneumonia and sepsis in these regions [3], [4].

The fulminating septicemic form of melioidosis has a mortality rate up to 90%, and overall mortality in northeastern Thailand remains at 40% despite over two decades of research and improved therapy [4]. In contrast, the mortality rate of B. pseudomallei infection in northern Australia is now comparatively low at 10–15% [3]. In addition, up to 5% of Australian melioidosis cases have a neurological presentation with an encephalomyelitis syndrome that rarely occurs in Thailand or elsewhere in Southeast Asia [3], [5], [6]. Despite the clear importance of B. pseudomallei and the disease it causes, the role of genetic variation in the B. pseudomallei genome and its association with disease severity has remained elusive.

Several ubiquitous virulence factors in B. pseudomallei have previously been identified, including the cytotoxin Burkholderia lethal factor 1 [7], capsular polysaccharide I [8], the cluster I type VI secretion system [9], and the Bsa type III secretion system cluster 3 [10]. However, the wide spectrum of melioidosis presentations has led to speculation that variably present virulence factors may influence disease severity. To examine this potential, we identified two variably present virulence candidates, determined their prevalence among 556 Australian B. pseudomallei strains of clinical origin collected over a 24-year period, and finally, compared their association with disease presentation and severity.

Results and Discussion

The first variable virulence candidate we examined was Burkholderia intracellular motility factor A (BimA), a putative type V effector protein [11] required for actin-based motility. BimA subverts host actin dynamics, enabling bacterial movement between eukaryotic cells and evasion of immune surveillance [11], [12]. All B. pseudomallei strains encode for BimA; however, approximately 12% of northern Australian strains possess a bimA variant that shares 95% homology with Burkholderia mallei (bimABm) [13], [14]. B. mallei is an equine-adapted clone of B. pseudomallei that causes glanders, a highly infectious disease primarily of horses but which is often fatal in humans if untreated [15]. BimABm shares only 54% homology with BimABp, although both variants impart actin polymerization capabilities [11]. The BimABp and BimABm carboxy-terminal ends share homology with the Yersinia autosecreted adhesion YadA [16], a critical virulence factor in Y. enterocolitica that enables host cell invasion while resisting immune attack [17].

Using multivariate logistic regression models to control for all potential confounders, we found a significant association between bimABm and neurological melioidosis (p<0.001), with patients infected by the bimABm variant being 14 times more likely to present with neurological involvement compared with patients infected with the bimABp variant (95% CI = 4.7–44.6). No covariates were found to be associated with neurological disease, indicating that bimABm was the only variable predictive of neurological presentation. In contrast, patients infected with B. pseudomallei possessing the bimABp variant were twice as likely to develop pneumonia compared with those infected with the bimABm variant (p = 0.028; 95% CI = 1.1–3.5), although several covariates existed for this presentation; specifically, being indigenous Australian Aboriginal, having chronic lung or heart disease, hazardous alcohol use, being older, not having a malignancy, and no history of kava use. Pneumonia is the most common melioidosis presentation in Australia, occurring in 51% of cases [3]; thus, the identification of multiple covariates for pneumonia is not unexpected. The bimABm variant has not yet been observed in clinical and environmental isolates from Thailand or in other endemic regions of Southeast Asia including Cambodia, Laos and Vietnam [14], although the bimABm variant has been found in two isolates originating from India [18]. Thus, the apparent absence of the bimABm variant in Southeast Asia could explain the reduced incidence of encephalomyelitis observed in this region.

Previous studies in a murine model have demonstrated that intranasal delivery of B. pseudomallei or B. mallei can result in invasive entry into the central nervous system, probably via the olfactory nerve, enabling the bacteria to directly infect the brain without hematogenous spread [19], [20]. In addition, neurological degeneration has been reported in a study on experimental glanders in horses [21]. Based on these studies, the prevailing hypothesis is that an intranasal route of infection substantially increases the risk of bacterial migration to the brain. We demonstrate that B. pseudomallei strains possessing the bimABm variant have a substantially heightened ability to cause central nervous system disease, possibly due to enhanced bacterial motility along the olfactory and other nerve pathways [20]. Although it remains unclear to what extent an inhalational/intranasal route of infection contributes to neurological melioidosis in humans, our findings suggest that inhalation or nasal aspiration of a bimABm variant substantially increases the likelihood of developing a brain infection compared with a bimABp variant.

The second variable virulence candidate we tested encodes for a filamentous hemagglutinin (FhaB). B. pseudomallei contains one ubiquitous FhaB (encoded by BPSS1727) [22] and three variably present FhaB loci. Of the latter, B. pseudomallei strains can contain one, two or all three fhaB genes [23]. These variably present genes are located on genomic islands (GIs), and previous reports have suggested a role in B. pseudomallei virulence [23], [24]. Of note, the variably present FhaB3 (encoded by BPSS2053 in B. pseudomallei K96243) is an anti-macrophage factor [25] involved in host epithelial cell attachment [24], the formation of multinucleated giant cells and actin-associated membrane protrusions [26]. BPSS2053 shares 47% homology with Bordetella pertussis FhaB, an adhesin that facilitates attachment to the host cell during infection, the secretion of which is dependent upon FhaC [27]. Due to its immunogenicity, FhaB from B. pertussis is as an essential component of the acellular pertussis (whooping cough) vaccine [28].

The majority (83%) of B. pseudomallei isolates examined in our study possessed fhaB3. Using multivariate logistic regression models to control for all potential confounders, we found that patients harboring fhaB3-positive B. pseudomallei were twice as likely to be blood culture-positive (p = 0.028; 95% CI = 1.1–3.4), although there was no significant association with septic shock or mortality. Several covariates existed for blood culture-positivity; namely, being indigenous Australian Aboriginal, being older, having diabetes, immunosuppression or malignancy, and hazardous alcohol use. In contrast, we observed a strong correlation between fhaB3 absence and cutaneous melioidosis without sepsis, with fhaB3-negative B. pseudomallei being four times more likely to be the infecting organism in these patients (p = 0.001; 95% CI = 1.8–8.1). Cutaneous melioidosis without sepsis occurs as the primary presentation in 13% of Australian cases [3], mostly in those with no identified risk factors and commonly in children; furthermore, fatalities have yet to be documented in this cohort [29].

It is intriguing that fhaB3 has, to date, been found in 100% of Thai B. pseudomallei strains [23] compared with only 83% of Australian strains, and as such it is tempting to speculate that fhaB3 may contribute to increased mortality in Thai melioidosis patients. However, our study did not find an association between fhaB3 and mortality. In addition, the potential contribution of fhaB3 to differential mortality rates between Thailand and Australia requires evaluation in the context of differences in access to diagnostic and therapeutic resources, and in particular, to state-of-the-art critical care management of severe sepsis [30]. Although further work is needed to identify other virulence determinants associated with mortality, it is striking that B. pseudomallei strains lacking fhaB3 are significantly less likely to cause severe disease. Indeed, it is possible that fhaB3, in concert with another virulence factor, contributes to adverse melioidosis outcomes.

In addition to the fhaB3 and bimA genes, we screened our isolate collection for the Burkholderia thailandensis-like flagellum and chemotaxis (BTFC) and the Yersinia-like fimbrial (YLF) gene clusters, which are mutually exclusive in the B. pseudomallei genome [31]. The BTFC and YLF gene clusters are putative virulence determinants, with the YLF cluster previously shown to be over-represented in clinical isolates [31]. Like fhaB3, the YLF cluster is more common in Thailand than Australia and as expected, we found that the majority of isolates in our study possessed the BTFC cluster (79%). We performed BTFC/YLF analysis across 543 strains from our clinical collection with the same parameters used for bimA and fhaB3 to assess correlation with disease presentation and severity metrics. Interestingly, no statistically significant correlations for clinical presentations or disease severity metrics were identified for BTFC or YLF (Table S3). There was, however, an overrepresentation of the YLF gene cluster in strains from remote regions of Australia. The lack of correlation of any clinical presentation or disease severity metric with BTFC/YLF in our Australian isolates is itself significant as it highlights the nonrandom nature of our bimA and fhaB3 findings.

Associations between variable virulence factors and adverse disease outcome are still poorly understood, although salient examples have been documented in well-studied human pathogens, most notably nontyphoid Salmonella [32] and Shiga-toxin producing Escherichia coli [33]. Our study has conclusively identified, for the first time, variably present B. pseudomallei virulence factors that are associated with specific melioidosis presentations. The bimABm variant was shown to be highly predictive of neurological melioidosis, and most likely explains why this disease presentation is more frequently observed in Australia than Thailand. Further, we have demonstrated that B. pseudomallei strains lacking fhaB3 are significantly associated with non-severe cutaneous disease compared with strains containing fhaB3, a finding which potentially contributes to the lower mortality rate of melioidosis in Australia. In contrast, strains containing fhaB3 were associated with positive blood cultures, but not septic shock or mortality, suggesting that additional virulence factors remain to be identified. In contrast, we did not find any association between BTFC/YLF and disease presentation or disease severity. Collectively, our findings represent a fundamental step towards understanding the molecular basis for variable disease presentation caused by this high-threat biological agent, and ongoing microbial genome-wide association studies are likely to uncover additional virulence signatures.

Materials and Methods

Ethics Statement

Our study examined primary B. pseudomallei isolates obtained from 556 melioidosis patients from the Darwin Prospective Melioidosis Study, which commenced at Royal Darwin Hospital, Northern Territory, Australia in October 1989. Ethics approval for this study was obtained through the Human Research Ethics Committee of the Northern Territory Department of Health and Menzies School of Health Research, approval number HREC 02/38 (Clinical and Epidemiological Features of Melioidosis) with written informed consent obtained from patients. All patient data were de-identified prior to analysis.

PCR Assay Design and PCR Conditions

The presence of fhaB3, bimABm, bimABp, BTFC and YLF among the B. pseudomallei isolates was determined using real-time PCR. For bimABm, 0.9 µM of each primer (AGCGCTTCGCGCATCTAC, CGCGTTAAACGCCGTACTTTC) (Invitrogen, Mulgrave, VIC, Australia) and 0.25 µM TaqMan minor groove-binding probe (6FAM-ATGCGCTCGCTATC) (Applied Biosystems, Foster City, CA, USA) was used to generate a 104 bp amplicon within this 579 bp gene (corresponding to BURPS668_A2118 in B. pseudomallei MSHR668). Identical primer (GGAAGCTTTGGCGTGCATAT, CCCATGCCTTCCTCGACTAAT) and probe (6FAM-ACGTCACTGCCAATC) concentrations were used to detect a unique 60 bp fragment within the 1.5 kb bimABp locus (encoded by BPSS1492 in B. pseudomallei K96243 [34]). For fhaB3 detection, 0.3 µM each primer (GACGCGGCACGTCTGATC, CGCGGATAAAACTCGGATTG) and 0.2 µM probe (6FAM-AACCAGGTCAACAGCA) was used to generate a conserved 58 bp product within the 9.3 kb fhaB3 gene (BPSS2053 in K96243). For BTFC and YLF detection, primers and probes were designed targeting lafU or BPSS0124 (corresponding to BTFC and YLF loci, respectively). BTFC and YLF detection was multiplexed into a single assay containing 0.3 µM of each primer (TGTTTCGCAGCGAGGATGTC, CCCACCGTCAAGCCGATT) and 0.2 µM of probe (6FAM-CGTCCGACCGAGTGC) targeting BTFC and identical concentrations of primers (GTGCCTGCAACGCTAATCG, CGCACTGATAGCCGGAATAGAG) and probe (VIC-ACTGGATTTACTCAGCGCAT) targeting YLF. Positive controls for bimABp, bimABm, BTFC, YLF or fhaB3 were included on each PCR plate to ensure accurate assay performance. All PCRs used TaqMan Universal Mastermix (Applied Biosystems) and were carried out to a final volume of 5 µL, in duplicate. PCR instrumentation and conditions were as previously described [35].

Clinical and Epidemiological Features of Melioidosis Cases

We categorized the 556 patients into six primary (and mutually exclusive) diagnoses: pneumonia, genitourinary involvement, blood culture positivity but no identifiable focus, localized skin infection without sepsis, neurological melioidosis and internal soft tissue abscess. Disease severity metrics were also included, where relevant: blood culture-positive growth of B. pseudomallei, septic shock, and mortality. Melioidosis risk factors were scored as either present or absent for the following, as previously defined for the Darwin Prospective Melioidosis Study [3]: chronic lung disease, chronic renal disease, diabetes, hazardous alcohol use, heart disease (defined as either rheumatic heart disease or congestive cardiac failure), immunosuppression, kava use and malignancy. Criteria for defining hazardous alcohol use, chronic lung disease, chronic renal disease and septic shock have previously been detailed [3]. Last, four epidemiological factors were included to rule out potential confounding influences: gender, ethnicity (either indigenous Australian Aboriginal or not), age, and probable acquisition of infection locality. Locality was divided into three regions: urban Darwin, rural Darwin, or elsewhere in Australia, including the remote Top End of the Northern Territory.

Statistical Analysis

All statistical analyses were carried out using Stata version 13.0 (StataCorp LP, College Station, TX, USA). Bivariate analysis was performed using the Pearson’s χ2 test where expected frequencies were >5 in all cells, or the two-sided Fisher’s exact test where expected frequencies were <5 in one cell (Tables S1 and S2). The Wilcoxon rank-sum test was used to compare age of diagnosis with bimA or fhaB3 status. To rule out confounding factors (e.g. age, geographic location, or risk factors) associated with bimA or fhaB3 status and clinical outcome, multivariate logistic regression analyses were performed using a stepwise backwards-elimination procedure as previously described [3]. We used a threshold of p = 0.1 for inclusion of covariates in the logistic regression model, followed by a p = 0.01 for primary associations and a p = 0.05 for secondary associations.

Acknowledgments

We are grateful to our microbiology laboratory and clinical colleagues at Royal Darwin Hospital for their support and expertise in identification of B. pseudomallei and in management of patients with melioidosis. We wish to thank our Menzies School of Health Research colleagues Leisha Richardson and Vanessa Theobald for laboratory assistance, and Mark Chatfield for statistical support.

Author Contributions

Conceived and designed the experiments: DSS EPP MK BJC. Performed the experiments: JRW MYV. Analyzed the data: DSS EPP JRW LMW MYV MK BJC. Contributed reagents/materials/analysis tools: DSS EPP AT MM MK BJC. Wrote the paper: DSS EPP BJC. Managed the isolate inventory: MM. Documented and collated all clinical data: BJC.

References

  1. 1. Butler D (2012) Viral research faces clampdown. Nature 490: 456.
  2. 2. Limmathurotsakul D, Dance DA, Wuthiekanun V, Kaestli M, Mayo M, et al. (2013) Systematic review and consensus guidelines for environmental sampling of Burkholderia pseudomallei. PLoS Negl Trop Dis 7: e2105.
  3. 3. Currie BJ, Ward L, Cheng AC (2010) The epidemiology and clinical spectrum of melioidosis: 540 cases from the 20 year Darwin prospective study. PLoS Negl Trop Dis 4: e900.
  4. 4. Limmathurotsakul D, Wongratanacheewin S, Teerawattanasook N, Wongsuvan G, Chaisuksant S, et al. (2010) Increasing incidence of human melioidosis in Northeast Thailand. Am J Trop Med Hyg 82: 1113–1117.
  5. 5. Limmathurotsakul D, Chaowagul W, Wongsrikaew P, Narmwong A, Day NP, et al. (2007) Variable presentation of neurological melioidosis in Northeast Thailand. Am J Trop Med Hyg 77: 118–120.
  6. 6. Currie BJ, Fisher DA, Howard DM, Burrow JN (2000) Neurological melioidosis. Acta Trop 74: 145–151.
  7. 7. Cruz-Migoni A, Hautbergue GM, Artymiuk PJ, Baker PJ, Bokori-Brown M, et al. (2011) A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A. Science 334: 821–824.
  8. 8. Reckseidler SL, DeShazer D, Sokol PA, Woods DE (2001) Detection of bacterial virulence genes by subtractive hybridization: identification of capsular polysaccharide of Burkholderia pseudomallei as a major virulence determinant. Infect Immun 69: 34–44.
  9. 9. Burtnick MN, Brett PJ, Harding SV, Ngugi SA, Ribot WJ, et al. (2011) The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei. Infect Immun 79: 1512–1525.
  10. 10. Wiersinga WJ, van der Poll T, White NJ, Day NP, Peacock SJ (2006) Melioidosis: insights into the pathogenicity of Burkholderia pseudomallei. Nat Rev Microbiol 4: 272–282.
  11. 11. Stevens MP, Stevens JM, Jeng RL, Taylor LA, Wood MW, et al. (2005) Identification of a bacterial factor required for actin-based motility of Burkholderia pseudomallei. Mol Microbiol 56: 40–53.
  12. 12. French CT, Toesca IJ, Wu TH, Teslaa T, Beaty SM, et al. (2011) Dissection of the Burkholderia intracellular life cycle using a photothermal nanoblade. Proc Natl Acad Sci U S A 108: 12095–12100.
  13. 13. Sitthidet C, Korbsrisate S, Layton AN, Field TR, Stevens MP, et al. (2011) Identification of motifs of Burkholderia pseudomallei BimA required for intracellular motility, actin binding, and actin polymerization. J Bacteriol 193: 1901–1910.
  14. 14. Sitthidet C, Stevens JM, Chantratita N, Currie BJ, Peacock SJ, et al. (2008) Prevalence and sequence diversity of a factor required for actin-based motility in natural populations of Burkholderia species. J Clin Microbiol 46: 2418–2422.
  15. 15. Srinivasan A, Kraus CN, DeShazer D, Becker PM, Dick JD, et al. (2001) Glanders in a military research microbiologist. N Engl J Med 345: 256–258.
  16. 16. Sitthidet C, Stevens JM, Field TR, Layton AN, Korbsrisate S, et al. (2010) Actin-based motility of Burkholderia thailandensis requires a central acidic domain of BimA that recruits and activates the cellular Arp2/3 complex. J Bacteriol 192: 5249–5252.
  17. 17. El Tahir Y, Skurnik M (2001) YadA, the multifaceted Yersinia adhesin. Int J Med Microbiol 291: 209–218.
  18. 18. Mukhopadhyay C, Kaestli M, Vandana KE, Sushma K, Mayo M, et al. (2011) Molecular characterization of clinical Burkholderia pseudomallei isolates from India. Am J Trop Med Hyg 85: 121–123.
  19. 19. Massey S, Johnston K, Mott TM, Judy BM, Kvitko BH, et al. (2011) In vivo bioluminescence imaging of Burkholderia mallei respiratory infection and treatment in the mouse model. Front Microbiol 2: 174.
  20. 20. Owen SJ, Batzloff M, Chehrehasa F, Meedeniya A, Casart Y, et al. (2009) Nasal-associated lymphoid tissue and olfactory epithelium as portals of entry for Burkholderia pseudomallei in murine melioidosis. J Infect Dis 199: 1761–1770.
  21. 21. Lopez J, Copps J, Wilhelmsen C, Moore R, Kubay J, et al. (2003) Characterization of experimental equine glanders. Microbes Infect 5: 1125–1131.
  22. 22. Chieng S, Carreto L, Nathan S (2012) Burkholderia pseudomallei transcriptional adaptation in macrophages. BMC Genomics 13: 328.
  23. 23. Tuanyok A, Leadem BR, Auerbach RK, Beckstrom-Sternberg SM, Beckstrom-Sternberg JS, et al. (2008) Genomic islands from five strains of Burkholderia pseudomallei. BMC Genomics 9: 566.
  24. 24. Sim SH, Yu Y, Lin CH, Karuturi RK, Wuthiekanun V, et al. (2008) The core and accessory genomes of Burkholderia pseudomallei: implications for human melioidosis. PLoS Pathog 4: e1000178.
  25. 25. Dowling AJ, Wilkinson PA, Holden MT, Quail MA, Bentley SD, et al. (2010) Genome-wide analysis reveals loci encoding anti-macrophage factors in the human pathogen Burkholderia pseudomallei K96243. PLoS One 5: e15693.
  26. 26. Kespichayawattana W, Rattanachetkul S, Wanun T, Utaisincharoen P, Sirisinha S (2000) Burkholderia pseudomallei induces cell fusion and actin-associated membrane protrusion: a possible mechanism for cell-to-cell spreading. Infect Immun 68: 5377–5384.
  27. 27. Clantin B, Delattre AS, Rucktooa P, Saint N, Meli AC, et al. (2007) Structure of the membrane protein FhaC: a member of the Omp85-TpsB transporter superfamily. Science 317: 957–961.
  28. 28. Poolman JT, Hallander HO (2007) Acellular pertussis vaccines and the role of pertactin and fimbriae. Expert Rev Vaccines 6: 47–56.
  29. 29. Gibney KB, Cheng AC, Currie BJ (2008) Cutaneous melioidosis in the tropical Top End of Australia: a prospective study and review of the literature. Clin Infect Dis 47: 603–609.
  30. 30. Cheng AC, West TE, Limmathurotsakul D, Peacock SJ (2008) Strategies to reduce mortality from bacterial sepsis in adults in developing countries. PLoS Med 5: e175.
  31. 31. Tuanyok A, Auerbach RK, Brettin TS, Bruce DC, Munk AC, et al. (2007) A horizontal gene transfer event defines two distinct groups within Burkholderia pseudomallei that have dissimilar geographic distributions. J Bacteriol 189: 9044–9049.
  32. 32. Fierer J, Guiney DG (2001) Diverse virulence traits underlying different clinical outcomes of Salmonella infection. J Clin Invest 107: 775–780.
  33. 33. Boerlin P, McEwen SA, Boerlin-Petzold F, Wilson JB, Johnson RP, et al. (1999) Associations between virulence factors of Shiga toxin-producing Escherichia coli and disease in humans. J Clin Microbiol 37: 497–503.
  34. 34. Holden MT, Titball RW, Peacock SJ, Cerdeno-Tarraga AM, Atkins T, et al. (2004) Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei. Proc Natl Acad Sci U S A 101: 14240–14245.
  35. 35. Kaestli M, Richardson LJ, Colman RE, Tuanyok A, Price EP, et al. (2012) Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens. J Clin Microbiol 50: 2059–2062.