A recent analysis suggested that historical climate forcing on the oceanic habitat of neonate sea turtles explained two-thirds of interannual variability in contemporary loggerhead (Caretta caretta) sea turtle nest counts in Florida, where nearly 90% of all nesting by this species in the Northwest Atlantic Ocean occurs. Here, we show that associations between annual nest counts and climate conditions decades prior to nest counts and those conditions one year prior to nest counts were not significantly different. Examination of annual nest count and climate data revealed that statistical artifacts influenced the reported 31-year lag association with nest counts. The projected importance of age 31 neophytes to annual nest counts between 2020 and 2043 was modeled using observed nest counts between 1989 and 2012. Assuming consistent survival rates among cohorts for a 5% population growth trajectory and that one third of the mature female population nests annually, the 41% decline in annual nest counts observed during 1998–2007 was not projected for 2029–2038. This finding suggests that annual nest count trends are more influenced by remigrants than neophytes. Projections under the 5% population growth scenario also suggest that the Peninsular Recovery Unit could attain the demographic recovery criteria of 106,100 annual nests by 2027 if nest counts in 2019 are at least comparable to 2012. Because the first year of life represents only 4% of the time elapsed through age 31, cumulative survival at sea across decades explains most cohort variability, and thus, remigrant population size. Pursuant to the U.S. Endangered Species Act, staggered implementation of protection measures for all loggerhead life stages has taken place since the 1970s. We suggest that the 1998–2007 nesting decline represented a lagged perturbation response to historical anthropogenic impacts, and that subsequent nest count increases since 2008 reflect a potential recovery response.
Citation: Arendt MD, Schwenter JA, Witherington BE, Meylan AB, Saba VS (2013) Historical versus Contemporary Climate Forcing on the Annual Nesting Variability of Loggerhead Sea Turtles in the Northwest Atlantic Ocean. PLoS ONE 8(12): e81097. doi:10.1371/journal.pone.0081097
Editor: Richard Reina, Monash University, Australia
Received: March 29, 2013; Accepted: October 16, 2013; Published: December 5, 2013
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Funding: These authors have no support or funding to report.
Competing interests: The authors have declared that no competing interests exist.
Across numerous disciplines, data are collected for the purpose of monitoring change and projecting trends. The value of these projections is contingent upon the quality of both the input data and the analytical model. Because inaccuracies in short-term model fits are compounded over time, the accuracy of long-term projections reflects the reliability of short-term predictors . As such, the number of temporal iterations upon which a projection is based is also of paramount importance. For demographic research, this caveat implies that population projections for long-lived, late maturing species may be less robust than for short-lived, early maturing species simply due to the number of years required to reach maturity.
Sea turtles are long-lived, late maturing species that are globally distributed but not adequately globally protected . Conant et al.  modeled life history parameters and annual survival rates for nine populations of loggerhead sea turtles (Caretta caretta) under the assumption of population growth, and concluded that this species was especially vulnerable to perturbations. Extrapolation of these life history parameters and annual survival rates for loggerhead sea turtles in the Northwest (NW) Atlantic Ocean suggests that only 0.2% of a given cohort remains by age 30 assuming a 5% (λ = 1.05) population growth rate. Because survivorship and reproductive values are highest for sea turtles that have reached or are approaching maturity , , protecting these life stages from anthropogenic threats such as commercial fishing is especially important .
Van Houtan and Halley  recently proposed an alternative to the long-held paradigm that the survivorship of large juvenile and adult sea turtles is more predictive of population change than juvenile recruitment. Although they did not exonerate fisheries mortality as an important threat, Van Houtan and Halley  suggested that cohort effects stemming from survival in the first year of life had a greater effect on population growth, concluding that this factor explained more than two-thirds of future interannual variability in loggerhead sea turtle nest counts in Florida. Their premise was that environmental conditions, based on examination of a 31-year lag in the Atlantic Multi-Decadal Oscillation (AMO), drive circulation patterns that in turn affect food availability and hatchling dispersal, and thus, hatchling survival . Because 80–90% of all nesting by this species in the NW Atlantic occurs in Florida , this conclusion holds substantial implications for subsequent management of this population .
The mechanism proposed by Van Houtan and Halley  was based on well-documented environmental influences on larval fish dispersal and survival ; however, hatchling sea turtles may be better equipped than larval fish to survive harsh conditions between ages 0 and 1 year. Many fish species have r-selected traits in that they mature early, have a high fecundity, and are short-lived, whereas all sea turtle species have mostly k-selected traits which include late maturity, lower fecundity (at least relative to fishes), and long lifetimes . Therefore, survival of young-of-the-year would affect adult recruitment states in fishes more so than in sea turtles, simply due to earlier maturity in most fishes. Although neonate survival within the nest environment (i.e., hatching success, emergence rate, poaching, predation, etc.) is likely to affect adult recruitment in sea turtles , , sea turtle adult recruitment is less sensitive to variable survival at the in-water neonate stage relative to fishes. Furthermore, because loggerhead sea turtle hatchlings are “low-energy float and wait foragers” , sea turtle hatchlings should be able to better withstand longer bouts of reduced food availability than larval fish.
In contrast to hatchlings, adult female sea turtles may be greatly influenced by environmental conditions, at least in regards to annual reproductive activity , , , , , . Not all adult female sea turtles in a population nest each year, which Hays  attributed to rates of energy consumption on the foraging grounds in the years prior to nesting. Accordingly, the 31-year lag effect reported by Van Houtan and Halley  may not actually capture age 0 survival, but rather, it may reflect the influence of winter and spring climate conditions on adult female energy uptake and thus, the number of annual nests laid. If true, contemporary environmental conditions may exert greater influence on annual nest counts than suggested by Van Houtan and Halley . With this hypothesis in mind, the first objective of the present study was to statistically evaluate the relative influence of the AMO, as well as two other important and inter-related climate indices in the northern hemisphere (i.e., the North Atlantic Oscillation (NAO) and the El Niño Southern Oscillation (ENSO) ), at both contemporary and historical intervals using the models considered by Van Houtan and Halley . A second objective was to test for differences in model fits using temporally partitioned annual nest count data to assess variability in model performance with respect to environmental metrics. A third objective was to assess the relative contribution of first time nesters (neophytes) to projected annual nest counts through the year 2043, which was suggested by Van Houtan and Halley  to be a mechanism for generational replication of cohort effects. Lastly, a survival matrix was created in order to quantitatively compare the relative importance of survival in the first year of life relative to the next 30 years before reaching maturity.
Data Selection and Manipulation
Annual loggerhead sea turtle nest count data at the 15 Florida index beaches analyzed by Van Houtan and Halley  were obtained directly from the Florida Fish and Wildlife Conservation Commission. All 15 beaches were located on the Atlantic coast of Florida, U S A between 28.7°N (Canaveral National Seashore) and 26.5°N (Boca Raton). Between 1989 and 2012, the period of record for the present study, these 15 beaches comprised an average of 67.6% of total annual nest counts reported from all beaches in Florida via the Statewide Nesting Beach Survey program (ABM, pers. obs). Because annual nest count data between 1989 and 2012 were normally distributed, actual values, rather than ln-transformed values , were analyzed. Given similar temporal trends across beaches , pooled annual nest count data were analyzed.
Unsmoothed (standard) monthly AMO data between 1856 and 2012 (long format) were uploaded from the Earth Systems Research Laboratory (ESRL) of the National Oceanographic and Atmospheric Administration (NOAA); http://www.esrl.noaa.gov/psd/data/timeseries/AMO/. Because the AMO index is based on sea surface temperature anomalies, reported values are dynamic rather than static. This practice results in high temporal variability for reported values; however, correspondence between data set versions also remains high. For example, annualized (by calendar year) AMO values analyzed in the present study differed from data analyzed by Van Houtan and Halley  by 1.9±35.6% (mean ± standard deviation [SD]); however, the Pearson’s correlation co-efficient (r) between the normalized versions of these two data sets (1856–2010) was 1.0. The AMO data were normalized as in Van Houtan and Halley  and confirmed through personal communication (K. Van Houtan, email, 2 January 2013): a single annual value was created as the mean of monthly values between January and June plus the preceding December, from which the grand mean was subtracted and the resulting value divided by the grand SD, in order to generate a normalized series with a mean of 0 and a SD of 1.
Gridded (1°×1°) monthly mean sea surface temperature (SST) data from the Optimal Interpolation (OI) version 2 satellite were obtained from the ESRL for the same spatial range (22–38°N, 72–84°W; http://www.esrl.noaa.gov/psd/data/gridded/data.noaa.oisst.v2.html ) specified by Van Houtan and Halley . This temporal range covered the majority of the geographic distribution of dispersing hatchlings, neritic juveniles, and adult loggerheads from the stock nesting principally on Florida beaches. We reproduced analyses of Van Houtan and Halley  by including only data for December in the year prior to nesting for grid cells identified by the ESRL as not containing land coverage (once data were averaged to a single yearly value).
Monthly values for the NAO and running tri-monthly values for the ENSO between 1950 and 2012 were uploaded from the Climate Prediction Center of the NOAA National Weather Service (http://www.cpc.ncep.noaa.gov/products/precip/CWlink/pna/nao.shtml; http://www.cpc.ncep.noaa.gov/products/analysis_monitoring/ensostuff/ensoyears.shtml). Despite both of these data sets being indices, values remained static over time; thus, unlike the AMO, original data, rather than normalized data, were used for analyses.
For objective 1, a series of hierarchical cluster analyses (Euclidean distance) were performed in Minitab 15® (Minitab, Inc., State College, Pennsylvania, U S A) to evaluate changes in fit between annual loggerhead sea turtle nest counts and AMO, NAO, and ENSO in the same year of nesting and lagged at annual intervals up to 39 years. Analysis of Variance (ANOVA; Minitab 15®) was used to test for differences in the distribution of percent similarity values among these three environmental indices. For all statistical tests, significance level (α) was 0.05. For each index, optimal lag intervals were selected based on maximum percent similarity at both a contemporary scale (<4 years) to encompass remigration rates  and at >10 years prior to nest count year to evaluate cohort effects. The later criteria was selected given few reports of age at maturity for loggerhead sea turtles in the NW Atlantic at <10 years .
Optimal contemporary and historical lag period influences on annual loggerhead sea turtle nest counts were evaluated using the four general linear models (Minitab 15®) of Van Houtan and Halley : Model 1 = linear fit, single parameter; Model 2 = curvilinear fit, single parameter; Model 3 = model 1+SST; Model 4 = model 2+SST. Parameter entry order was also evaluated as part of the process for selecting a best contemporary lag and best historical lag parameter for subsequent model testing. A paired t-test (Minitab 15®) was used to compare predicted and actual nest counts (1989–2012) based on the best contemporary and historical lag parameters.
General linear models (Minitab 15®) were also used to evaluate the relative influence of the optimal contemporary lag parameter, optimal historical lag parameter, and both parameters combined. These evaluations were conducted for the full 24-year data set, as well as with the data set portioned into two groups denoting the first and last 12 years to compare model fits before and during a precipitous nest count decline . Parameter fits were evaluated using significance (P-value) and model fits were described by model-adjusted coefficient of determination (r2); because Van Houtan and Halley (2011) reported the correlation coefficient (r) for actual vs. predicted nest counts in lieu of r2, we also provide this metric in our tables.
For objective 2, annual nest count data were filtered as two, three, and four year series to evaluate the role of these remigration intervals on interannual variability in nest counts, and in turn, model performance. For each remigration interval, nest counts recorded in 1989 were assigned to the first data series, with the second data series beginning in 1990 and so forth. In order to further evaluate whether optimal contemporary and historical lag parameters were consistently significant throughout the data set, annual nest count data for each series for each remigration interval were described using the best fit equation selected in objective 1.
For objective 3, we applied the life history parameters and annual survival rates reported by Conant et al.  for a 5% (λ = 1.05) population growth rate to annual nest counts between 1989 and 2012, in order to calculate the age 31 neophyte nesting population during 2020–2043. The neophyte nesting population at age 31 was computed as follows: nest count in the year of cohort origin ×57.5 female eggs per nest ×54% hatchling emergence ×70% hatchling frenzy survival (initial dispersal) ×40% age 0 survival ×79.4% annual survival during the next 10 years ×89.3% annual survival during the next 19 years ×95% survival during the 31st year, with continued 95% annual survival thereafter. Conant et al.  did not incorporate survival for the hatchling frenzy ; however, we conservatively estimated it as 70% for this brief but important gauntlet based loosely on published observations . We acknowledge that inclusion of this additional mortality gauntlet reduces cohort first year survival by seven percent and in turn alters modeled population growth rates  which are beyond the scope of this study. Also, because the purpose of this analysis was to focus exclusively on changes in future abundance under the assumption of consistency among developing cohorts, we disregarded potential interannual differences in cohort sex ratios  and assumed a 1:1 female to male ratio .
In order to evaluate the relative importance of the future neophyte population, we also quantified the size of the remigrant population using the metrics of Conant et al. . Assuming a clutch frequency of five nests per season, we estimated that 11,367 mature female loggerheads nested in 2012. Given a remigration interval of three-years, we multiplied the estimated number of female nesters in 2012 by three to generate a total remigrant female loggerhead population of 34,102 individuals in 2012. Because of the highly variable interannual trends in loggerhead sea turtle nest counts in the past decade, we selected three different scenarios for calculating the remigrant population at the start of the 2020 nesting season: (A) unchanged from 2012, (B) following a 20% annual increase between 2013 and 2019 consistent with interannual trends between 2007 and 2012, and (C) following a 20% annual decrease between 2013 and 2019 for further context. The relative composition of neophytes in 2020 was computed as the number of neophytes surviving to age 31 divided by the sum of these neophytes plus one-third of the total remigrant population alive at the start of the 2020 nesting season, which was 5% less than in 2019. In subsequent years, neophyte nesters from the previous year were included in the remigrant population size calculation after applying the 95% annual survival rate. Reproductive longevity for this species is not well documented, but limited tag-recapture data suggest it may span >25 years , which is longer than the duration of the annual nest count data set considered herein.
For objective 4, a survival matrix was created to compare the relative effects of survival in the first year of life (3 stages) vs. the next 30 years using 12 mean survival rates: 1%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, and 99%. Cell values in this matrix denoted the proportion (to 0.00%) of the cohort that remained after 31 years. Maximum annual nest count discrepancies in the 24-year data set for the 15 Florida beaches were extrapolated to evaluate the maximum net increase in annual survival needed to temper cohort size disparity by age 31.
Objective 1: Contemporary vs. Historical Model Fits
The distribution of percent similarity values between annual loggerhead sea turtle nest counts (1989–2012) and environmental indices in the same year or lagged annually by up to 39 years were significantly different (F2 = 8.31, P<0.001) among environmental indices (Figure 1). The NAO was associated with a higher overall percent similarity (mean ±SD = 64±7%) than either the AMO (56±15%) or ENSO (57±8%). Optimal contemporary and historical lag associations for the NAO occurred in March of the year prior to nesting (79% similarity) and in September 20 years prior to nesting (77% similarity), respectively. Lag associations for the AMO and the ENSO always occurred in the last step of each cluster analysis; thus, the original normalized value (AMO) or twelve month annualized (ENSO) values were used. Optimal contemporary AMO (53% similarity) and ENSO (65% similarity) lags occurred the year of nesting and three years prior to nesting, respectively. Optimal historical AMO (85% similarity) and ENSO (77% similarity) lags occurred 32 years and 33 years prior to nesting, respectively.
Figure 1. Associations between climate indices and loggerhead (Caretta caretta) sea turtle nesting on Florida index beaches.
Percent similarity (y-axis) between the NAO (blue line) and annual nest counts at 15 Florida index beaches (1989 to 2012) peaked a year prior to nesting; however, percent similarity for the AMO (red line) and the ENSO (green line) and annual nest counts peaked at 32 and 33 years prior to nesting, respectively.doi:10.1371/journal.pone.0081097.g001
At contemporary time-scales, the NAO in March of the year prior to the year of nesting was the only significant (P = 0.003) parameter in model 1, where it accounted for 34% of the sums of squares (Table S1). The SST term was only significant in models 3 and 4 when the NAO was excluded; thus, the NAO in March of the year prior to nesting was substituted for SST as the contemporary model parameter of choice.
At historical lag scales, the normalized AMO 32 years prior to the year of nesting was the only significant (P<0.001) parameter in model 1, where it accounted for 44% of the sums of squares (Table S1). The AMO was a non-significant (P>0.05) term in models 2 and 4, but accounted for a high (and variable depending on entry order) proportion of the sums of squares (Table S1). Because the square of negative and positive values disproportionately relate to their original values, which greatly affected the AMO residuals (), we only analyzed the AMO using the linear model instead of AMO+AMO2 as in the curvilinear.
Contemporary NAO was a significant model term overall (P = 0.006), but not when the data set was partitioned into 12-year spans (Table 1); however, model fits for contemporary NAO were similar during both periods (Figure 2a). Historical AMO was a significant (P<0.001) model term overall and after 2001 (P = 0.009), but was non-significant (P = 0.744) during 1989–2000 (Figure 2b). Annual nest counts predicted by the contemporary NAO only vs. the historical AMO only during 1989–2012 were not significantly different (T = 0.0, P = 1.000; Figure 2c). The greatest co-efficient of determination (adjusted r2 = 0.53) and thus the best model fit was observed when both the contemporary NAO and the historical AMO were included (Figure 2c).
Figure 2. Fitted (A,B) and predicted (C) loggerhead (Caretta caretta) sea turtle nests on Florida index beaches.
Annual nest counts predicted by contemporary NAO only (blue line), historical AMO only (red line), or both parameters combined (green line) were not statistically different. In all panels, observed annual nest counts for loggerhead (Caretta caretta) sea turtle at 15 Florida index beaches during 1989–2012 are displayed as black lines.doi:10.1371/journal.pone.0081097.g002
Table 1. Descriptive statistics for predictions of loggerhead sea turtle (Caretta caretta) nest counts at 15 Florida index beaches during 1989 to 2012 based on historical (AMO) and contemporary (NAO) environmental influences.doi:10.1371/journal.pone.0081097.t001
Objective 2: Remigration Interval Influence
The historical AMO 32 years prior to nest counts was only a significant model term for one data series for each of the three remigration intervals evaluated (Table 1, Figure 3). Equation term entry order resulted in high variability in the percent of sums of squares accounted for by the historical AMO (Table 1). With the exception of the four year remigration interval for the series beginning in 1991, for which the historical AMO accounted for 82–84% of the sums of squares and the greatest co-efficient of determination value was observed (adjusted r2 = 0.87), comparable percentages of sums of squares were accounted for by the historical AMO regardless of term significance (Table 1). The NAO in March of the year prior to nesting was never a significant model term for the filtered data set; however, for at least one series in each remigration interval, the NAO accounted for between 40–73% of the sums of squares (Table 1).
Figure 3. Remigration interval effects on loggerhead sea turtle (Caretta caretta) nest counts on Florida index beaches.
Observed nest counts (A) were filtered into two (B), three (C), and four (D) year data series to evaluate remigration interval effects on interannual variability in nest counts and model performance. Data series orders for panels B–D were assigned as follows: first (red), second (dark blue), third (green), fourth (light blue). The multi-decadal shift between high (1998) and low (2007) annual nest counts was a common feature to the original and the filtered data series; however, inconsistencies in the interannual signal among the filtered data series within each remigration interval demonstrate the importance of contemporary environmental conditions on annual sea turtle nest counts.doi:10.1371/journal.pone.0081097.g003
Objective 3: Neophyte Importance and Nesting Projections
Under the assumption of consistent stage-based and age-based survival across cohorts hatched on 15 Florida index beaches during 1998–2012, between 2,689 and 5,740 neophyte females were projected to recruit to the nesting population annually during 2020–2043 (Table S2). The greatest (62%, Figure 4a) projected proportion of annual nests associated with neophyte nesters occurred in 2020 under the assumption of a 79% decline in the remigrant population in 2019 (scenario C). Conversely, if nest counts in 2019 were unchanged from 2012 (scenario A) or 258% greater than 2012 (scenario B), neophyte nesters were only projected to account for 26% to 9% of nest counts in 2020, respectively. Systematic decline in the importance of neophyte nesters was projected through 2034 for scenarios A and B, at which point neophyte nester stabilized and accounted for 15–19% of annual nest counts (Figure 4a) concomitant with stable remigrant nesting by 15,000 to 20,000 females annually (Figure 4b). Because 5% annual loss of remigrant females under scenario B removed more females annually than were replaced by neophyte recruits, a slight but systematic increase in the importance of neophyte recruits was projected under scenario B (Figure 4a). Reduced neophyte recruitment during 2029–2038 in turn exacerbated the rate of decline in the remigrant population during the same period (Figure 4b).
Figure 4. The relative importance of neophyte (A) and remigrant (B) nesters.
Neophyte recruitment at 15 Florida index beaches during 2020–2043 was computed using observed nest counts at these beaches during 1989–2012 and assuming consistent stage- and age-based survival through age 31 across cohorts. Remigrant population size for the same period was evaluated under a range of scenarios contingent upon nesting in 2019. In scenario A (blue lines), nesting in 2019 was assumed to be the same as in 2012. In scenario B (green lines), nesting in 2019 was 258% greater than in 2012 following 20% annual increases after 2012. In scenario C (red lines), nesting in 2019 was 79% less than in 2012 following 20% annual decreases beginning in 2013.doi:10.1371/journal.pone.0081097.g004
Annual nest counts projected for loggerhead sea turtles at these 15 Florida index beaches during 2020–2043 reflect the aforementioned temporal trends in remigrant population size (Figure 5). If nesting in 2019 was equal to that reported in 2012 (scenario A), annual nest counts were projected to steadily increase from 72,713 in 2020 to a high of 123,021 in 2031, decrease by 6.3% to 115,230 nests in 2038, then increase again to 131,711 nests in 2043 (Figure 5). If nesting in 2019 was 79% less than reported in 2012 (scenario C), annual nest counts were projected to steadily increase from 30,044 in 2020 to 98,751 in 2031, remain generally steady through 2038, and then systematically increase to 118,597 nests in 2043 (Figure 5). Only under the assumption of 258% greater nesting in 2019 relative to 2012 (scenario B) did annual nest counts systematically decline through 2043 (Figure 5), with the greatest decline (16%) noted between 2029 (204,342 nests) and 2038 (170,365 nests), corresponding to reduced recruitment from neophytes hatched between 1998 and 2007.
Figure 5. Projected (2020–2043) loggerhead (Caretta caretta) sea turtle nest counts on Florida index beaches.
Age 31 neophyte nest counts during 2020–2043 were extrapolated from observed nest counts (grey bars) at 15 Florida index beaches during 1989–2012 under the assumption of consistent stage-based and age-based survival across all cohorts. Remigrant nest counts were evaluated for three scenarios in which nest counts in 2019 were unchanged from 2012 (scenario A, blue line), 258% greater than 2012 (scenario B, green line), and 79% less than 2012 (scenario C, red line). Under these three scenarios, which are also based on 5% annual population growth, projected annual nest counts for these 15 beaches would exceed the demographic recovery criteria for the entire Peninsula Recovery Unit of 106,100 nests per year (black line) by 2020, 2026, and 2041, respectively.doi:10.1371/journal.pone.0081097.g005
Objective 4: Survival Matrix Evaluation
A maximum difference of 114% existed between loggerhead sea turtle nest counts recorded at 15 Florida index beaches between 1998 (58,880) and 2007 (27,513). However, if net survival for the 2007 cohort was 3.8% greater annually relative to the 1998 cohort during the next 30 years, it would be possible for these two cohorts to have equal abundance by age 31. Described by the age 0 vs. age 1 to 31 survival matrix (Table 2), high mortality experienced during three survival gauntlets (hatching emergence, crawl frenzy, aquatic hatchling stage) in the first year of life may be offset by improvements in annual survival during the next 30 years. For example, 95% cohort survival through the first year of life coupled with 80% annual survival for the next 30 years preserves the same proportion of a cohort at age 31 (0.11%) as would exist for a cohort that experienced 30% survival through the first year of life but 90% annual survival for the next 30 years (Table 2).
Table 2. An overview of cohort survival to age 31, whereby columns denote a range of survival probabilities during three gauntlets in the first year of life (i.e., hatchling emergence, hatchling frenzy, and survival at sea) and rows denote a range of annual at-sea survival probabilities during the next 30 years.doi:10.1371/journal.pone.0081097.t002
In the past decade, considerable emphasis has been placed on assessing relationships between climate and population regulation of long-lived sea turtle species. Most climate-related studies have focused on changes in sex ratios as they would be affected by incubation temperature . The role of temperature on sea turtle nesting has also been investigated with respect to reconstruction of historical incubation temperatures  and the potential future variation in spatio-temporal nest distribution and/or hatchling fitness , , , , . Associations between temperature and food availability for adult females have also been suggested to be linked to interannual variability in nesting activity for most sea turtle species , , , , , . Alternatively, Van Houtan and Halley  suggested that most of the interannual variability in loggerhead sea turtle nest counts in Florida between 1989 and 2010 could be explained by climate forcing on hatchling survival. In the present study, we reached greatly different conclusions than those reached by Van Houtan and Halley , and herein highlight important analytical considerations that influenced the results of both studies.
Interannual variability in nest counts explained by the NAO index in March of the year prior to nesting was not statistically different from the variability explained by the winter and spring AMO 32 years earlier. This observation was not in agreement with the 31-year AMO lag influence reported by Van Houtan and Halley . We attribute this disparity to the following factors: (1) in the present study the number of historical and contemporary lag terms was equal whereas the curvilinear model of Van Houtan and Halley  included twice as many historical as contemporary terms; (2) only significant model terms were included in our models; and (3) the contemporary term selected in the present study (i.e., March NAO) had a superior overall model fit than the contemporary term (i.e., December SST) used by Van Houtan and Halley . Although the NAO was a significant model term overall, the relationship between NAO and annual nest counts was strongest prior to the 41% decline in annual nest counts which occurred between 1998 and 2007 . Reduced performance of the March NAO after 2001 likely stemmed from reduced variability in nest counts concomitant with increased variability in the March NAO during 2004–2009. This assertion is supported by enhanced performance of the lagged AMO index during 2004–2009, when interannual variability in the lagged AMO was at its absolute minimum during the 24 years of data evaluated.
We hypothesize that improved performance of the lagged AMO metric vs. the contemporary NAO metric with respect to remigration intervals may also be attributed to data variance. Unlike the NAO, the AMO was a significant model term for at least one subset of data analyzed for each remigration interval, with the best fit observed for a four-year remigration interval series which began in 1991. However, closer inspection of this data set (Figure 2d) revealed inconsistencies in the relationship between variation in annual nest counts and variation in the lagged AMO. For example, between 1995 and 1999 the lagged AMO varied by 0.70, but corresponded to a less than 3% decline in annual nest counts. Between 1999 and 2002 the AMO index was predominantly associated with values approaching zero; however, nest counts declined by 32%. Conversely, the greatest interannual change (i.e., a decline of 2.62) in the AMO occurred between 2002 and 2003 when nest counts increased slightly (6%). Similarly, only a modest change (0.11) in the lagged AMO was observed between 2003 and 2007 when there was a 30% decline in annual nest counts. Lastly, between 2007 and 2011, annual nest counts increased by nearly as much as they decreased between 2003 and 2007, but the difference in change in the lagged AMO between these periods was ten-fold. In summary, despite the strong linear trend between the lagged AMO and annual nest counts for loggerhead sea turtles on 15 Florida index beaches, we suggest that this observation represents a spurious relationship due to the multi-decadal cycle of the AMO. This chance co-occurrence brought about a strong fit between extreme nest counts and AMO metrics, but otherwise resulted in inconsistent relationships.
Prior to 2001, observed nest counts were generally greater than nest counts predicted based on contemporary NAO, historical AMO, or both of these parameters; however, the opposite relationship persisted between 2001 and 2009, particularly for the NAO only model.
Reduced performance of all environmental indices models during the period of nest count decline suggests a lagged response to population perturbations which occurred decades prior. Although survival of all life history stages is important, our survival matrix demonstrated that survival at sea over 30 years has a greater cumulative influence on cohort abundance at age 31 than survival exclusively in the first year of life. This conclusion concurs with population modeling by Conant et al.  who also cautioned on the perils of accumulation of even small declines in survival across decades, as well as the need for high annual survival rates at sea to maintain positive population growth, as previously suggested by Leslie-Matrix models , .
When observed nest count data were partitioned into multiple data series at selected remigration intervals, all series exhibited evidence of stabilization (if not recovery) following a decline. Prior to the decline in annual nest counts during 1998–2007, one series (with different data) for both the two-year and four-year remigration intervals exhibited considerably less variability than the others. Although it is highly unlikely that all nesting sea turtles in a given year are on the exact remigration schedule , , this finding is intriguing given that prior to the decline, the NAO was a marginally non-significant term that accounted for one-third of annual nest count variation. We attribute the pre-decline nest count associations to have driven the significance of the NAO in the overall model, but also note that the NAO, the AMO, and the ENSO are all teleconnected climatically. Therefore, these climate teleconnections also explain the associations of all three climate indices with the annual nest counts reported in Figure 1.
Loggerhead sea turtles were added to the Endangered Species Act in 1978 (FWS and NMFS 1978, 43 FR 32800) and it is reasonable to presume that this listing was preceded by practices that negatively affected loggerhead survival for some time prior to species listing. Conservation efforts on Florida beaches began prior to the listing , but characterization of in-water threats and subsequent implementation of mitigation measures were not initiated until several decades later , , . Therefore, given that the lowest annual nest count data for loggerhead sea turtles on Florida index beaches occurred 30 years after their receipt of federal protection, we cautiously suggest that the decline during 1998–2007 represents a lagged perturbation effect. This scenario has been demonstrated for leatherbacks nesting in Pacific Costa Rica where illegal, historical egg poaching, that was estimated to affect nearly 90% of all nests, had a lagged response on nesting numbers . We further suggest that recent increases in Florida loggerhead annual nest counts since 2008 may reflect lagged protection benefits, especially given steady increases in catch rates for loggerhead sea turtles approaching maturity since 2000  and that exhibit a strong genetic association with regional nesting assemblages .
Nesting projections for 2020 to 2043 under a range of nesting population scenarios also reinforce the suggestion that 1998–2007 decline in nesting resulted from differential survival and/or female production across cohorts several decades prior. Because these projections were based on the assumption of consistent survival rates and reproductive traits across cohorts, neophyte recruitment during 2020–2043 was projected to mirror nesting trends during 1989–2012; however, because neophytes were projected to generally account for a small portion of nesting during 2020–2043, the precipitous decline in nesting during 1998–2007 was not projected to recur 31 years later. Furthermore, our projections suggest that unless nesting declines appreciably between 2013 and 2019 relative to 2012 and if the population was growing in accordance with a 5% trajectory, annual nesting at the 15 index beaches we analyzed would attain the 106,100 annual nest count demographic recovery criteria for the entire Peninsular Recovery Unit of the Northwest Atlantic Distinct Population Segment  by 2027. Lastly, because we assumed a conservative 1:1 female to male sex ratio versus the highly female-biased ratios reported for loggerhead sea turtles in the North Atlantic , , , there is an opportunity for our nest count projections to be underestimates.
On multiple foraging grounds throughout the southeast U.S., catch rates and size distributions have increased for a range of loggerhead sea turtle size classes during the past two decades , , , . These changes were attributed to recruitment of strong year classes that successfully passed through the survival gauntlet; however, without an age structure for these sampled foraging grounds, support for increased recruitment remains weak. Age assessments are routinely conducted for dead stranded sea turtles because skeletochronology, the most promising technique demonstrated thus far, is an invasive procedure that requires a full cross section of the humerus . Although this technique could be used to track the temporal prevalence of age classes, interpretation of trends presents a challenge given that a temporal decline in the occurrence of stranded age × turtles could at least in part be influenced by increased annual survival rates at sea. Size classes are often used as a proxy for age, but this practice is also not ideal because growth rates fluctuate spatially and temporally  as well as decline with age . Because environmental conditions vary across decades, as substantiated by the indices examined in the present study, the development of reliable and minimally-invasive methods for conducting large-scale age assessments should be a high priority for future research and funding.
Satellite telemetry has revealed that loggerhead sea turtles captured at mating aggregations  and nesting assemblages ,  in the Northwest Atlantic Ocean consist of both resident and migratory individuals. We hypothesize that individuals located further from breeding areas have greater migration energy requirements, and likewise suggest that resident individuals have shorter remigration intervals than their migrant counterparts. Hamann et al.  suggested that variable reproductive remigration intervals among individuals may reflect differences in the uptake of energy needed to reach a critical threshold body condition. Using stable isotopes, which offer a reliable and nominally-invasive means to distinguish between oceanic and neritic foraging grounds , , Hatase et al.  demonstrated longer remigration intervals for oceanic female loggerhead sea turtles than their neritic counterparts. Stable isotopes, in conjunction with satellite telemetry, have also been used to differentiate neritic foraging grounds across latitudinal gradients , . On a limited basis, Pajuelo et al.  also reported interannual variability in the contribution of nesting females from various foraging grounds. We suggest that annual isotope characterizations, coupled with diet studies and somatic growth assessments, could also greatly improve capabilities for future nest count projections. As such, we encourage continued efforts to use stable isotopes to monitor interannual variability in the contributions of adult female sea turtles from various foraging grounds to annual nest counts. Bjorndal et al.  recently reported a temporal decline in growth rates for juvenile loggerheads corresponding to the period of reduced nesting activity in Florida , further reinforcing the potential link between energy uptake and subsequent annual nesting activity.
Visualization of the disproportionate response of squaring (blue bars) negative vs. positive values for the normalized AMO index (red bars), and the subsequent temporal disparity for comparison with annual nest counts (black line) for loggerhead (Caretta caretta) sea turtles on 15 Florida index beaches between 1989 and 2012.
Descriptive statistics (mean, SD, coefficient of variation (CV), minimum, and maximum) for the percent sums of square values associated with each model parameter examined in the four models considered by Van Houtan and Halley . Model parameters (AMO, NAO, ENSO) were evaluated at lagged intervals to quantify contemporary (<4 years before nest count) and cohort (>10 years after nest count) influences. Model coefficient of determination (adjusted r2) and significance (P-value) are also provided.
Estimated total and remigrant female loggerhead (Caretta caretta) sea turtle population sizes during 1989–2012 and projected (2020–2043) neophyte, total, and remigrant adult female population sizes under selected scenarios. In scenario A, the remigrant population size was unchanged from 2012. In scenario B, the remigrant population size was 258% greater than in 2012 following 20% annual increases in nest counts during 2013–2019. In scenario C, the remigrant population size was 79% less than in 2012 following 20% annual decreases in nest counts during 2013–2019. For all three scenarios, the total adult female population in each year between 2020 and 2043 was computed as the compounded sum of a 5% annual decline in an initial (2019) remigrant pool plus a subsequent 5% annual decline in each neophyte cohort size between 2020 and 2043 after initial nesting. Given a three-year remigration interval, annual remigrants were computed as one-third of each annual adult female population size estimate between 2020 and 2043.
We offer sincere thanks to the individuals who collected and managed these long-term data sets, as well as express our gratitude to the FWC and NOAA for making their data sets available. Discussions with D. Bagley and S. Ceriani (UCF) and W. Jenkins (SCDNR) regarding cohort effects and long-term nesting cycles sustained critical thought for this manuscript. We thank H. Haas (NMFS) and journal reviewers for their considerable improvements to this manuscript. This is SCDNR MRD Contribution No. 710.
Conceived and designed the experiments: MDA JAS VSS BEW ABM. Performed the experiments: MDA JAS. Analyzed the data: MDA JAS. Contributed reagents/materials/analysis tools: MDA JAS VSS. Wrote the paper: MDA JAS VSS. Provided nest count data: BEW ABM. Approved of the analytical format in advance: BEW ABM. Contributed editorially to manuscript completion: BEW ABM.
- 1. Judd K, Small M (2000) Towards long-term prediction. Phys D 136: 31–44. doi: 10.1016/s0167-2789(99)00152-9
- 2. Wallace BP, Dimatteo AD, Hurley BJ, Finkbeiner EM, Bolten AB, et al. (2010) Regional management units for marine turtles: A novel framework for prioritizing conservation and research across multiple scales. PLoS ONE 5: e15465 doi:10.1371/journal.pone.0015465.
- 3. Conant TA, Dutton PH, Tomoharu E, Epperly SP, Fahy CF, et al.. (2009) Loggerhead sea turtle (Caretta caretta) 2009 status review under the U.S. Endangered Species Act. Silver Spring: Loggerhead Biological Review Team, National Marine Fisheries Service. 222 p.
- 4. Crouse DT, Crowder LB, Caswell H (1987) A stage-based population model for loggerhead sea turtles and implications for conservation. Ecology 68: 1412–1423. doi: 10.2307/1939225
- 5. Crowder LB, Crouse DT, Heppell SS, Martin TH (1994) Predicting the impact of Turtle Excluder Devices on loggerhead sea turtle populations. Ecol Appl 4: 437–445. doi: 10.2307/1941948
- 6. Wallace BP, Lewison RL, McDonald SL, McDonald RK, Kot CY, et al. (2010) Global patterns of marine turtle bycatch. Conserv Letters 3: 131–142. doi: 10.1111/j.1755-263x.2010.00105.x
- 7. Van Houtan KS, Halley JM (2011) Long-term climate forcing in loggerhead sea turtle nesting. PLoS ONE 6: e19043 DOI:10.371/journal.pone.0019043.
- 8. Witherington B, Kubilis P, Brost B, Meylan A (2009) Decreasing annual nest counts in a globally important loggerhead sea turtle population. Ecol Appl 19: 30–54. doi: 10.1890/08-0434.1
- 9. Platt T, Fuentes-Yaco C, Frank KT (2003) Spring algal bloom and larval fish survival. Nature 423: 398–399. doi: 10.1038/423398b
- 10. Musick JA (1999) Ecology and conservation of long-lived marine animals. In: Life in the slow lane: Ecology and conservation of long-lived marine animals: Bethesda: American Fisheries Society Symposium Musick JA, editor. 23: 1–7. doi: 10.1086/393692
- 11. Santidrián Tomillo P, Saba VS, Blanco GS, Stock CA, Paladino FV, et al. (2012) Climate driven egg and hatchling mortality threatens survival of Eastern Pacific leatherback turtles. PLoSONE 7: e37602 doi:10.1371/journal.pone.0037602.
- 12. Saba VS, Stock CA, Spotila JR, Paladino FV, Santidrián-Tomillo P (2012) Predicted response of an endangered marine turtle population to climate change. Nature Clim Change 2: 814–820. doi: 10.1038/nclimate1582
- 13. Witherington BE (2002) Ecology of neonate loggerhead turtles inhabiting lines of downwelling near a Gulf Stream front. Mar Biol 140: 843–853. doi: 10.1007/s00227-001-0737-x
- 14. Limpus CJ, Nicholls N (2000) ENSO regulation of Indo-Pacific green turtle populations. In: Hammer GL, Nicholls N, Mitchell C, editors. The Australian Experience: Dordrecht: Kluwer Academic Publishers. 399–408.
- 15. Solow AR, Bjorndal KA, Bolten AB (2002) Annual variation in nesting numbers of marine turtles: the effect of sea surface temperature on re-migration intervals. Ecol Letters 5: 742–746. doi: 10.1046/j.1461-0248.2002.00374.x
- 16. Saba VS, Santidrián-Tomillo P, Reina RD, Spotila JR, Musick JA, et al. (2007) The effect of the El Niño Southern Oscillation on the reproductive frequency of eastern Pacific leatherback turtles. J Appl Ecol 44: 395–404. doi: 10.1111/j.1365-2664.2007.01276.x
- 17. Chaloupka M, Kamesaki N, Limpus C (2008) Is climate change affecting the population dynamics of the endangered Pacific loggerhead sea turtle? J Exp Mar Biol Ecol 356: 136–143. doi: 10.1016/j.jembe.2007.12.009
- 18. Reina RD, Spotila JR, Paladino FV, Dunham AE (2009) Changed reproductive schedule of eastern Pacific leatherback turtles Dermochelys coriacea following the 1997–1998 El Niño to La Niña transition. Endang Species Res 7: 155–161. doi: 10.3354/esr00098
- 19. Monte-Luna P, Guzman-Hernandez V, Cuevas EA, Arrenguin-Sanchez F, Lluch-Belda D (2012) Effect of North Atlantic climate variability on hawksbill turtles in the Southern Gulf of Mexico. J Exp Mar Bio Ecol 412: 103–109. doi: 10.1016/j.jembe.2011.11.005
- 20. Hays GC (2000) The implications of variable remigration intervals for the assessment of population size in marine turtles. J Theor Bio 206: 221–227. doi: 10.1006/jtbi.2000.2116
- 21. Huang J, Higuchi K, Shabbar A (1998) The relationship between the North Atlantic Oscillation and the El Niño–Southern Oscillation. Geophys Res Lett 25: 2707–2710. doi: 10.1029/98gl01936
- 22. Avens L, Snover ML (2013) Age and age estimation in sea turtles. In Wyneken J, Lohmann KJ, Musick JA, editors. The Biology of Sea Turtles, Volume 3: Boca Raton: CRC Press. 97–134.
- 23. Dial BE (1987) Energetics and performance during nest emergence and the hatchling frenzy in loggerhead sea turtles (Caretta caretta). Herpetologica 43: 307–315.
- 24. Salmon M, Witherington BE (1995) Artificial lighting and seafinding by loggerhead hatchlings: Evidence for lunar modulation. Copeia 1985: 931–938. doi: 10.2307/1447042
- 25. Godfrey MH, Mrosovsky N, Barreto R (1996) Estimating past and present sex ratios of sea turtles in Suriname. Can J Zool 74: 267–277. doi: 10.1139/z96-033
- 26. Dahlen MK, Bell R, Richardson JI, Richardson TH (2000) Beyond D-0004: Thirty-four years of loggerhead (Caretta caretta) research on Little Cumberland Island, Georgia, 1964–1997. In Abreu-Grobois FA, Briseno-Duenas R, Marquez R, Sarti L, editors. Proceedings of the Eighteenth International Sea Turtle Symposium: Silver Spring: NOAA Technical Memorandum NMFS-SEFSC-436: 60–62.
- 27. Standora EA, Spotila JR (1985) Temperature dependent sex determination in sea turtles. Copeia 1985: 711–722. doi: 10.2307/1444765
- 28. Hays GC, Broderick AC, Glen F, Godley BJ (2003) Climate change and sea turtles: a 150-year reconstruction of incubation temperatures at a major marine turtle rookery. Global Change Biol 9: 642–646. doi: 10.1046/j.1365-2486.2003.00606.x
- 29. Hawkes LA, Broderick AC, Godfrey MH, Godley BJ (2007) Investigation the potential impacts of climate change on a marine turtle population. Global Change Biol 13: 923–932. doi: 10.1111/j.1365-2486.2007.01320.x
- 30. Witt MJ, Hawkes LA, Godfrey MH, Godley BJ, Broderick AC (2009) Predicting the impacts of climate change on a globally distributed species: the case of the loggerhead turtle. J Exp Biol 213: 901–911. doi: 10.1242/jeb.038133
- 31. Fuentes MMPB, Limpus CJ, Hamann M (2010) Vulnerability of sea turtle nesting grounds to climate change. Global Change Biol 17: 140–153. doi: 10.1111/j.1365-2486.2010.02192.x
- 32. Santidrián Tomillo P, Saba VS, Piedra R, Paladino FV, Spotila JR (2008) Effects of illegal harvest of eggs on the population decline of leatherback turtles in Las Baulas Marine National Park, Costa Rica. Conserv Biol 22: 1216–1224. doi: 10.1111/j.1523-1739.2008.00987.x
- 33. Witzell WN (1999) Distribution and relative abundance of sea turtles caught incidentally by the U.S. pelagic longline fleet in the western North Atlantic Ocean, 1992–1995. Fish Bull 97: 200–211.
- 34. Watson JW, Epperly SP, Shah AK, Foster DG (2005) Fishing methods to reduce mortality associated with pelagic longlines. Can J Fish Aq Sci 62: 965–981. doi: 10.1139/f05-004
- 35. Arendt MD, Schwenter JA, Boyton J, Segars AL, Byrd JI, et al. (2012) Temporal trends (2000–2011) and influences on fishery- independent catch rates for loggerhead sea turtles (Caretta caretta) at an important coastal foraging region of the Southeast U.S. Fish Bull. 110: 470–483.
- 36. Arendt MD, Boynton J, Schwenter JA, Segars AL, Byrd JI, et al. (2012) Spatial clustering of loggerhead sea turtles in the Northwest Atlantic: implications for management surveys. Endang Species Res 18: 219–231. doi: 10.3354/esr00450
- 37. National Marine Fisheries Service and U.S. Fish and Wildlife Service (2008) Recovery plan for the Northwest Atlantic population of the loggerhead sea turtle (Caretta caretta), 2nd rev. Silver Spring: National Marine Fisheries Service. 325 p.
- 38. Wibbels TA, Martin RE, Owens DW, Amoss MSJ Jr (1991) Female-biased sex ratio of immature loggerhead sea turtles inhabiting the Atlantic coastal waters of Florida. Can J Zool 69: 2973–2977. doi: 10.1139/z91-419
- 39. Delgado C, Canário, Dellinger T (2010) Sex ratios of loggerhead sea turtles Caretta caretta during the pelagic stage. Mar Biol 157: 979–990. doi: 10.1007/s00227-009-1378-8
- 40. Arendt MD, Schwenter JA, Segars AL, Byrd JI, Maier PP, et al. (2012) Catch rates and demographics of loggerhead sea turtles (Caretta caretta) captured from the Charleston, South Carolina, shipping channel during the period of mandatory use of turtle excluder devices (TEDs). Fish Bull 110: 98–109.
- 41. Ehrhart LM, Redfoot WE, Bagley DA (2007) Marine turtles of the central region of the Indian River Lagoon System, Florida. Florida Sci 70: 415–434.
- 42. Epperly SP, J Braun-McNeill, P.M Richards (2007) Trends in catch rates of sea turtles in North Carolina, USA. Endang Species Res 3: 283–293. doi: 10.3354/esr00054
- 43. Bjorndal KA, Schroeder BA, Foley AM, Witherington BE, Bresette M, et al. (2013) Temporal, spatial, and body size effects on growth rates of loggerhead sea turtles (Caretta careta) in the Northwest Atlantic. Mar Biol 160: 2711–2721. doi: 10.1007/s00227-013-2264-y
- 44. Heppell SS, Snover ML, Crowder LB (2003) Sea turtle population ecology. In Wyneken J, Lohmann KJ, Musick JA, editors. The Biology of Sea Turtles, Volume 2: Boca Raton: CRC Press. 275–306.
- 45. Arendt MD, Segars AL, Byrd JI, Boynton J, Whitaker JD, et al. (2012) Distributional patterns of adult male loggerhead (Caretta caretta) sea turtles in the vicinity of Cape Canaveral, Florida, USA during and after a major annual breeding aggregation. Mar Biol 159: 101–112. doi: 10.1007/s00227-011-1793-5
- 46. Hawkes LA, Witt MJ, Broderick AC, Coker JW, Coyne MS, et al. (2011) Home on the range: spatial ecology of loggerhead turtles in Atlantic waters of the USA. Divers and Distrib 17: 624–640. doi: 10.1111/j.1472-4642.2011.00768.x
- 47. Ceriani SA, Roth JD, Evans DR, Weishampel JF, Ehrhart LM (2012) Inferring foraging areas of nesting loggerhead turtles using satellite telemetry and stable isotopes. PLoS ONE 7: e45335 doi:10.1371/journal.pone.0045335.
- 48. Hamann M, Limpus CJ, Owens DW (2003) Reproductive cycles of males and females. In Wyneken J, Lohmann KJ, Musick JA, editors. The Biology of Sea Turtles, Volume 2: Boca Raton: CRC Press. 135–162.
- 49. Caut S, Guirlet E, Angulo E, Das K, Girondot M (2008) Isotope analysis reveals foraging area dichotomy for Atlantic leatherback turtles. PLoS ONE 3: e1845 doi:10.1371/journal.pone.001845.
- 50. Hatase H, Omuta K, Tsukamoto K (2010) Oceanic residents, neritic migrants: a possible mechanism underlying foraging dichotomy in adult female loggerhead turtles (Caretta caretta). Mar Bio 157: 1337–1342. doi: 10.1007/s00227-010-1413-9
- 51. Hatase H, Tuskamoto K (2008) Smaller longer, larger shorter: energy budget calculations explain intrapopulation variation in remigration intervals for loggerhead sea turtles (Caretta caretta). Can J Zool 86: 595–600. doi: 10.1139/z08-035
- 52. Pajuelo M, Bjorndal KA, Reich KJ, Vander Zanden HB, Hawkes LA, et al. (2012) Assignment of nesting loggerhead turtles to their foraging areas in the Northwest Atlantic using stable isotopes. Ecosphere 3: 1–18. doi: 10.1890/es12-00220.1