B. cenocepacia is an opportunistic human pathogen that is particularly problematic for patients suffering from cystic fibrosis (CF). In the CF lung bacteria grow to high densities within the viscous mucus that is limited in oxygen. Pseudomonas aeruginosa, the dominant pathogen in CF patients, is known to grow and survive under oxygen-limited to anaerobic conditions by using micro-oxic respiration, denitrification and fermentative pathways. In contrast, inspection of the genome sequences of available B. cenocepacia strains suggested that B. cenocepacia is an obligate aerobic and non-fermenting bacterium. In accordance with the bioinformatics analysis we observed that B. cenocepacia H111 is able to grow with as little as 0.1% O2 but not under strictly anoxic conditions. Phenotypic analyses revealed that H111 produced larger amounts of biofilm, pellicle and proteases under micro-oxic conditions (0.5%–5% O2, i.e. conditions that mimic those encountered in CF lung infection), and was more resistant to several antibiotics. RNA-Seq and shotgun proteomics analyses of cultures of B. cenocepacia H111 grown under micro-oxic and aerobic conditions showed up-regulation of genes involved in the synthesis of the exopolysaccharide (EPS) cepacian as well as several proteases, two isocitrate lyases and other genes potentially important for life in micro-oxia.
Data deposition: RNA-Seq raw data files are accessible through the GEO Series accession number GSE48585. MS data have been deposited in the ProteomeXchange database (PXD000270).
Citation: Pessi G, Braunwalder R, Grunau A, Omasits U, Ahrens CH, et al. (2013) Response of Burkholderia cenocepacia H111 to Micro-Oxia. PLoS ONE 8(9): e72939. doi:10.1371/journal.pone.0072939
Editor: Tom Coenye, Ghent University, Belgium
Received: May 30, 2013; Accepted: July 15, 2013; Published: September 2, 2013
Copyright: © 2013 Pessi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was financially supported by the Swiss National Science Foundation (Project 31003A-143773) to LE and the Swiss SystemsX.ch initiative (grant IPP 2011/121) to CHA and LE. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Burkholderia cenocepacia is one of the 17 members of the Burkholderia cepacia complex (Bcc) whose extraordinary metabolic versatility allows it to adapt to a variety of environmental conditions, including infection sites in humans , . Of particular concern are lung infections of patients suffering from cystic fibrosis (CF). One of the major problems associated with Bcc infections is their capacity to form highly organized surfaced-associated communities (biofilms) with an intrinsic resistance to most common antibiotics in clinical use , . Several strains of the Bcc species B. multivorans, B. cenocepacia, B. cepacia, and B. dolosa have been shown to be highly transmissible between patients , with B. cenocepacia and B. multivorans accounting for the majority of CF infections . During chronic colonization of the CF lung, bacteria are under strong selective pressures that result from challenges of the immune defense, antimicrobial therapy, nutrient and oxygen availability . B. cenocepacia produces biofilms and uses the highly viscous mucus of the CF lung as a rich nutrient source. Due to bacterial respiration a steep oxygen gradient within the mucus is generated and the deeper layers become anaerobic –. This observation is supported by the fact that anaerobes have been found to occur in CF sputum at high cell densities . Recently, Alvarez-Ortega and colleagues provided evidence that the major CF pathogen P. aeruginosa is growing in the CF lung preferentially by micro-oxic respiration . Moreover, chemostat experiments with aerobic and micro-oxic cultures of P. aeruginosa suggested that this facultative anaerobe is growing optimally in a micro-oxic environment where it is producing more virulence factors such as the exopolysaccharide (EPS) alginate and pyocyanine . Further studies have also shown that an anaerobic environment stimulates the production of alginate , . In anaerobiosis, P. aeruginosa can utilize nitrate or nitrite rather than oxygen as a terminal electron acceptor –. In the absence of nitrate or nitrite, it can convert arginine to ornithine, thereby generating energy for anoxic growth –. Finally P. aeruginosa can use pyruvate fermentation for long-term survival of up to 18 days under anoxic conditions and this conversion of pyruvate into lactate, acetate, and succinate is in turn inhibited by nitrate respiration .
In a retrospective study of a Burkholderia dolosa outbreak among CF patients, the genomes of 112 isolates collected from 14 individuals over 16 years were sequenced and intriguingly revealed that 3 out of the 17 genes found to be under strong selection during pathogenesis had mutations in genes involved in oxygen-dependent regulation . This suggests that sensing of a low oxygen environment is critical for pathogenesis in lung infections.
These findings posed the question of how B. cenocepacia, which is considered an obligate aerobe, can grow or survive in the micro-oxic/anoxic CF lung environment. Very recently, Sass and colleagues reported a low-oxygen activated locus (lxa) that has been shown to play an important role in regulation of the low oxygen response in B. cenocepacia strains J2315 and K56-2 . After exposure to an anoxic environment, the lxa mutant showed less viable cells compared to the wild type. However, the B. cenocepacia H111 strain, which was originally isolated from a CF patient as well as other B. cepacia complex (Bcc) strains, do not possess the lxa locus , .
Here we show that B. cenocepacia H111 as well as other B. cenocepacia strains did not display any obvious functions that would allow anaerobic growth: no genes were found that are involved in denitrification and arginine fermentation. However, H111 is able to grow at an oxygen concentration of 0.1%, yet cannot grow anoxically in culture. When grown micro-oxically, B. cenocepacia produces more substratum-associated biofilm mass as well as a more robust pellicle compared to aerobic conditions. Finally, RNA-Seq and shotgun proteomics analyses from matched aerobic and micro-oxic samples were carried out to obtain a more detailed view of the repertoire of genes and proteins potentially important for growth in a low oxygen environment.
Growth of B. cenocepacia H111 at different oxygen concentrations
The ability of B. cenocepacia to grow at different oxygen concentrations in complex media was tested. When cells were grown under normal aerobic conditions (21% O2), the cells grew to an optical density (OD600) of around 3 with a generation time of approximately 65 minutes. When only 5% or 0.5% oxygen was supplied (see Methods), the cells grew slower (generation times of 113 and 180 minutes, respectively), probably because the dissolved oxygen concentration dropped quickly to growth limiting levels (Figure 1). However, B. cenocepacia was still able to grow with 0.1% oxygen with a doubling time of 268 minutes, reaching an OD600 of 0.7. To test for growth in the absence of oxygen, several alternative electron acceptors, including nitrate, fumarate, and DMSO as well as the C-sources pyruvate, oxalate and arginine and a medium mimicking synthetic mucus , were tested. However, in none of the conditions tested did we observe growth in the absence of oxygen.
Figure 1. Growth of B. cenocepacia at different oxygen concentrations (21%, 5%, 0.5% and 0.1%).
Aerobic cultures (21%, black line) were grown with shaking in 1L Erlenmeyer flasks containing 100 ml LB medium while micro-oxic cultures were grown in 500-ml rubber-stoppered serum bottles containing 25 ml LB medium in presence of a nitrogen gas atmosphere that contained 5% (grey line), 0.5% (grey dashed line) or 0.1% (grey dotted line) oxygen (Pangas). Whiskers indicate SD, n = 3.doi:10.1371/journal.pone.0072939.g001
Identification of genes in the B. cenocepacia H111 genome that may be required for growth under “low-oxygen” conditions
To identify genes related to denitrification, arginine fermentation or pyruvate fermentation, we searched for the corresponding P. aeruginosa orthologs in B. cenocepacia H111 and other sequenced Burkholderia species. The genes required for denitrification which encode all enzymes for nitrate/nitrite, nitric-oxide and nitrous-oxide reduction (PA3872-75, PA0509-PA0519, PA0520-24, PA3391-96), could only be identified in the “pseudomallei” group members B. thailandensis, B. pseudomallei and B. mallei. Indeed B. pseudomallei was reported to be able to survive without oxygen using nitrate respiration , . In contrast, B. cenocepacia strains, including strain H111 were found to only possess the nitrite reductase encoding gene cluster (BCAM1683-86). P. aeruginosa is also able to generate ATP by the degradation of arginine to ornithine, which requires expression of the arcABC operon (PA5170-73) . While arcB (ornithine carbamoyltransferase) is present in all sequenced B. cenocepacia strains, the entire operon is only present in strains of B. thailandensis, B. pseudomallei, B. mallei, B. ambifaria, B. xenovorans, B. phymatum and B. phytofirmans. The fermentation of pyruvate can also be used by P. aeruginosa to generate energy and survive during anoxic growth (PA0835-36 and PA0927) . The genes necessary for the conversion of pyruvate to lactate, acetate, and succinate, i.e. the acetate kinase ackA, the phosphate acetyltransferase pta and the lactate dehydrogenase ldhA, were found in the genome of all Burkholderia species. Many bacteria adapt to micro-oxic conditions by synthetizing a particular cytochrome c oxidase (cbb3) complex with a high affinity for oxygen –. No classical cbb3 cytochrome oxidase was found in any of the sequenced Burkholderia strains. In contrast, a homolog of the bd-type oxidase (cyanide insensitive) was identified in the genome of several Burkholderia strains including strain H111 (BCAM2674-75). Homologs of the P. aeruginosa central regulator of anaerobic metabolism FNR/ANR (PA1544)  were identified in all sequenced Burkholderia strains. The strain H111 has two FNR/ANR orthologs, BCAM0049 and BCAM1483.
Micro-oxic conditions favor the sessile lifestyle
The capacity of our model strain H111 to form a biofilm in a polystyrene microtiter dish-based assay was tested under aerobic and micro-oxic conditions. The biofilm index (BI), i.e. biofilm mass normalized against planktonic growth, was used to compensate for the different growth rates. The amount of adhered biomass in cells grown to the begin of stationary phase was found to be significantly higher with 0.5% oxygen (Biofilm Index 80%) compared to 21% oxygen (Biofilm index 55%) (p-value<0.01, Figure 2). We also tested for pellicle formation, i.e. the biofilm formed at the liquid-air interface of static cultures and B. cenocepacia was found to produce more pellicle under micro-oxic conditions (data not shown). Other phenotypes such as swarming and swimming motility were not affected by oxygen availability after 48 hours of incubation. In contrast, the production of siderophores as measured on CAS plates was reduced in micro-oxically grown cells (Figure S1).
Figure 2. Influence of oxygen on biofilm formation in B. cenocepacia H111.
Biofilm formation in ABC minimal medium. B. cenocepacia H111 was grown in 96-well plates under aerobic (black) or in micro-oxic (grey) conditions created in a CampyGen compact system (oxoid). Whiskers indicate SD, n = 3.doi:10.1371/journal.pone.0072939.g002
The production of extracellular factors such as cellulases, proteases, lipases, was also investigated. These assays revealed that proteolytic activity was significantly higher under micro-oxic conditions (p-value<0.01, Figure 3) while lipolytic and cellulolytic activities remained constant and were independent of the oxygen level. To examine whether cells that were grown with low oxygen were also more resistant to antibiotics, we exposed cells grown micro-oxically and aerobically on plates to the aminoglycosides kanamycin, gentamycin and to tetracycline. Cells grown micro-oxically showed an increased resistance to all tested aminoglycosides as well as to tetracycline (Figure 4).
Figure 3. Protease activity is increased in micro-oxia.
The exoenzymes cellulase, protease and lipase were measured in supernatants of aerobic (black) and micro-oxic (grey) growing cells as described in material and methods. The activity in the supernatant of aerobic cells was set to 100%. Whiskers indicate SD, n = 6.doi:10.1371/journal.pone.0072939.g003
Figure 4. Oxygen-dependent antibiotic resistance profile of B. cenocepacia H111.
Discs containing 30 µg Kanamycin, 30 µg Tetracycline or 10 µg Gentamycin, respectively, were placed on a plate containing B. cenocepacia H111 strain. Plates were incubated aerobically (black) or micro-oxically (grey) and mean halo diameters were determined. Whiskers indicate SD, n = 3.doi:10.1371/journal.pone.0072939.g004
Oxygen availability affects metabolic pathways
The metabolism of B. cenocepacia H111 grown under aerobic and micro-oxic conditions was compared using Biolog plates for carbon (C) and nitrogen (N) utilization. In these assays the strain's ability to oxidize 190 carbon and 95 nitrogen substrates was tested. An overview of all significant differences in C and N-source utilization under aerobic versus micro-oxic conditions is presented in Table 1. Interestingly, H111 was able to metabolize approximately 70% of the tested C- sources and around 90% of the investigated N-sources. We observed that micro-oxic cells grew to a 4-fold higher optical density (OD600) on inosine and to a 2-fold greater OD on adenosine, tricarballylic acid, malonic acid and succinamic acid compared to aerobically growing cells. For the utilization of N-sources we found a 2-fold increased respiration of ethylendiamine and D, L-α- amino-caprylic acid under micro-oxia.
Table 1. List of carbon- and nitrogen compounds that were differentially used in micro-oxic versus aerobic conditions.doi:10.1371/journal.pone.0072939.t001
Global transcript and protein expression changes in response to low oxygen
To investigate the underlying molecular mechanisms of the observed phenotypic alterations under micro-oxic conditions we performed a transcriptomic as well as a proteomic analysis. For a global profiling of transcript and protein levels, aerobic and micro-oxic cells were grown to the late exponential phase (OD600 of 0.8 and 0.4, respectively, Figure 1). Total protein extracts and RNA were obtained from matched samples and further processed (see Methods). To enable detection of low abundance proteins, samples were subfractionated and analyzed using an exclusion list approach . The analysis of cytoplasmic, extracellular and membrane fractions identified a total of 2128 proteins (1726 in oxia, 1911 in micro-oxia). We used DESeq  to generate a list of differentially expressed proteins (or genes, see below), ranked according to statistical significance (see Methods). Of the top 58 differentially expressed proteins (Figure 5) the majority (41) were up-regulated in micro-oxia. A global transcript profile analysis of the same samples identified 3806 and 4133 genes expressed aerobically and micro-oxically, respectively. Of the 123 top differentially expressed genes identified by DESeq, 102 were up-regulated in micro-oxia. Importantly, of the 58 differentially expressed proteins, 51 were also found to be similarly regulated at the transcript level. Altogether, we obtained a list of 176 genes and/or proteins that were differentially regulated by low-oxygen (Table 2). Among them, 139 genes/proteins (78%) were up-regulated in micro-oxia, including several transporters (BCAL0447, BCAS0081, BCAS0451, BCAS0602) and outer membrane proteins, genes involved in synthesis of the EPS cepacian (BCAM1004-1005 and BCAM1010), several proteases (Table 2) and an isocitrate lyase (ICL, BCAL2118). Several genes/proteins involved in reactive oxygen species (ROS) scavenging such as catalases, the alkyl hydroperoxide reductase AhpC and several thioredoxins showed increased expression in low-oxygen conditions. Among the highly up-regulated transcriptional regulators was the FNR-type regulator BCAM0049 as well as the rpoS homolog BCAM1259. A functional classification based on proNOG categories of the EggNOG resource  (see Methods) revealed that genes/proteins involved in post-translational modification, protein turnover and chaperones (category O) are over-represented in the list of genes/proteins that are up-regulated by low oxygen. In contrast, the functional categories “cell motility (category N)” and “inorganic ion transport and metabolism (category P) are enriched in the dataset of genes/proteins down-regulated in micro-oxia.
Figure 5. Differential protein expression under micro-oxic and aerobic conditions.
MA plot showing the log2 fold change in protein expression of B. cenocepacia H111 grown under micro-oxic versus aerobic conditions. The top regulated proteins are shown in color: proteins with increased expression under micro-oxic conditions are indicated in red, down-regulated proteins in green.doi:10.1371/journal.pone.0072939.g005
Table 2. List of 176 B. cenocepacia H111 genes/proteins that showed differential expression in micro-oxic (M) conditions compared to aerobic (A) conditions (DESeq analysis, p-value<0.15 for proteomics and p-value<0.2 for RNA-Seq).doi:10.1371/journal.pone.0072939.t002
To further validate the global analysis data, the up-regulation of several genes was confirmed by qPCR (Table S3). These included up-regulation of BCAM0049 and BCAM1259 expression as well as increased expression of the protease gene BCAL1919 (clpB), the cytochrome d ubiquinol kinase gene BCAL0785, the sugar transferase gene involved in cepacian synthesis (BCAM1010) and the ICL encoding gene (BCAL2118). In addition, transcriptional lacZ fusions to promoter regions of selected genes up-regulated in micro-oxic conditions were constructed and measured (Figure S2). The promoter of a gene involved in cepacian biosynthesis (sugar transferase wcaJ), the thioredoxin BCAL2780, the rpoS homolog (BCAM1259) and the lectin encoding gene BCAM0185 showed increased activity when cells were grown with low oxygen (Figure S2). As a control we used PcepI-lacZ transcriptional fusion and confirmed that the expression of the AHL encoding gene cepI was not affected by oxygen availability (confirming our RNA-Seq data).
At present very little is known of how B. cenocepacia strains can adapt to the micro-oxic/anoxic environment within biofilms in the CF lung –, . While the CF pathogen P. aeruginosa uses denitrification and fermentation of arginine to generate energy for growth and survival in an environment depleted of oxygen , , we could only detect very few orthologs of the respective genes involved in these processes in the genomes of B. cenocepacia strains. Denitrification genes were exclusively found in the genomes of members of the “pseudomallei” group, namely B. thailandensis, B. pseudomallei and B. mallei. Only strains of B. thailandensis, B. pseudomallei, B. mallei, B. ambifaria, B. xenovorans, B. phymatum and B. phytofirmans, harbor genes that potentially allow these species to ferment arginine to gain energy (1 mol of ATP per mol of arginine). In accordance with these findings it has been reported that the diversity of Burkholderia strains growing under anoxic conditions in soils is very low .
The facultative intracellular pathogen Mycobacterium tuberculosis has recently been shown to adapt to and recover from hypoxia using isocitrate lyase (ICL)-mediated production of succinate . ICL is a glyoxylate shunt enzyme, which generates succinate whose secretion was proposed to help maintain membrane potential and ATP synthesis. The produced succinate is also a substrate of the succinate dehydrogenase (SDH) in the TCA cycle which is important for the electron transport chain by coupling carbon flow to ATP synthesis . The gene encoding ICL has been shown to be up-regulated in persister cells in B. cenocepacia biofilms . The authors of this study suggested that surviving persister cells downregulate the TCA cycle to avoid production of ROS and at the same time activate an alternative pathway, the glyoxylate shunt. Employing a combined RNA-Seq and proteomics approach we found that the two ICL genes present in the H111 genome as well as genes/proteins involved in ROS scavenging such as catalases, AhpC and several thioredoxins were up-regulated by low oxygen. Given that the same genes were also up-regulated in micro-oxia in strain J2315 , it is tempting to speculate that this pathway is used by B. cenocepacia to sustain production of ATP under micro-oxic conditions.
A phenotypical characterization revealed that micro-oxic cells grew better with purines as C-source. The up-regulation of two adenosine deaminases (Table 2 and Table S1) which are key enzymes of purine metabolism and convert adenosine to inosine suggest a role of purine metabolism in micro-oxia. Interestingly, a recent report on hepatocarcinoma-derived cells showed that purines such as inosine and adenosine have a cytoprotective effect and can serve as an alternative source of energy to produce ATP during hypoxic conditions . The ribose moiety of adenosine and purine could be used as a precursor for the phosphorylated glycolytic intermediates in reactions catalyzed by the pentose phosphate (PP) pathway. Among the genes up-regulated in micro-oxia (Table S1) we also found several nucleoside phosphorylases which catalyse the reversible phosphorolysis of purine (2′-deoxy)ribonucleosides to free bases and (2′-deoxy)ribose 1-phosphates. This could represent another possibility for B. cenocepacia to generate energy under micro-oxic conditions.
In this study we showed that micro-oxic conditions promoted biofilm formation of B. cenocepacia. Similar observations have been made for P. aeruginosa, which produces more alginate when oxygen is limiting –. Our global expression analyses revealed the up-regulation of three regions potentially responsible for increased biofilm formation under micro-oxic conditions: i) the EPS cepacian encoding gene cluster BCAM1004-10 ,  ii) the lectin gene BCAM0185 , and iii) the gene encoding the large surface protein BapA, which was previously shown to be important for biofilm formation .
The observation that cells growing micro-oxically were more resistant to several antibiotics is probably due to their slower growth rate compared to aerobically growing cells. Muir et al. showed that the higher the growth rate of cells at the time of antibiotic addition, the greater the growth-inhibitory effect .
The effect of low-oxygen tension on gene expression was one of the nine conditions tested by Sass and colleagues in B. cenocepacia strain J2315 . Although the experimental settings used in their study were very different from ours (i) shift versus run out experiment, ii) CampyGen Compact gas generating system versus controlled gas atmosphere, iii) 6% versus 0.5% oxygen, iv) strain J2315 versus H111) and different analysis technologies were used (microarray versus RNA-Seq), there was a good overlap between the two data sets. In fact, 55 of the 176 H111 genes/proteins reported here were also differentially expressed in response to low oxygen in strain J2315 (Table S2). Among them are universal stress proteins, the protease ClpB, the isocitrate lyase BCAL2118, arginine/ornithine decarboxylases, the cytochrome d ubiquinol oxidase and several membrane proteins. In line with the observation that strain H111 produces reduced amounts of siderophores in micro-oxia, several TonB dependent receptors were down-regulated in micro-oxic conditions. The lxa locus as well as the cable pilus cluster (cbl), which are both induced in strain J2315, are absent in strain H111. Other gene clusters for flagellar and chemotaxis proteins were up-regulated only in strain J2315. Among the genes specifically induced in strain H111 we found the fimbriae encoding gene fimA (BCAL1677), an adenosine deaminase (BCAM2458), several porins (BCAM2723, BCAL3007, BCAM2462) and several ABC transporters. Among the transcriptional regulators highly up-regulated in micro-oxia in both studies was the FNR-type regulator BCAM0049 (Table 2). Orthologous proteins have been shown to sense the oxygen tension and control gene expression under low oxygen conditions in several organisms , . The P. aeruginosa FNR-type regulator ANR is known to positively control expression of denitrification and arginine fermentation genes. This regulator could also play an important role in the regulation of genes in micro-oxic conditions.
In conclusion, we have shown that B. cenocepacia H111 can grow with as little as 0.1% oxygen but is not able to grow anaerobically. Since P. aeruginosa grows anaerobically and has been shown to occupy deeper sites within wounds  it appears likely that B. cenocepacia may occupy a different niche where oxygen is limited but not totally absent. Our study provides a list of the most significant differentially expressed genes/proteins in micro-oxically versus aerobically grown cells and opens new avenues in the understanding of the molecular mechanism underlying the physiology and regulation of the in vivo relevant micro-oxic lifestyle of B. cenocepacia.
Materials and Methods
Bacterial strains, plasmids and growth conditions
B. cenocepacia wild type H111 , ,  was grown under aerobic (21% oxygen) and micro-oxic conditions (0.1% to 5% oxygen) at 37°C in LB Lennox broth (Difco) or ABC Minimal Medium containing citrate as carbon source . Aerobic cultures were grown with rigorous shaking (220 rpm) in 500-mL Erlenmeyer flasks containing 25 ml medium or, for RNA-Seq and proteomics experiments, in 1-L Erlenmeyer flasks containing 100 ml medium. Micro-oxic liquid cultures were grown under a nitrogen gas atmosphere that contained 5% or 0.5% or 0.1% oxygen with moderate shaking (80 rpm) in 500-ml rubber-stoppered serum bottles containing 50 ml medium. The gas phase (e. g 0.5% O2, 99.5% N2) was exchanged every 8–14 hours. For the cultivation of bacteria on plates, micro-oxic conditions were created using the CampyGen Compact gas generating system (oxoid) by quickly changing the paper sachet every 24 hours and keeping the exposure to atmospheric oxygen at a minimum.
Biofilm formation was quantified in a microtiter dish assay as described by Huber et al. . Since micro-oxic and aerobic cells reached different optical densities (OD), we used the biofilm index (BI) to compare the amounts of biofilm formed. The BI was calculated as the mean percentage ratio between OD570 after crystal violet staining and OD550 measured before incubating the cells with crystal violet which reflects the total cell number . The formation of pellicles was assessed in NYG medium (0.5% peptone, 0.3% yeast extract, 2% glycerol) according to Fazli et al., 2011 . Proteolytic activity was quantified based on the method described by Schmid et al  growing cells in NYG medium at 37°C to late exponential growth phase and using azocasein (5 mg/ml, in 50 mM Tris-Cl pH 8) for 60 min at 37°C as substrate. For quantification of lipases and cellulases, the sterile culture supernatant was incubated with buffer 1 (1 volume 0.3% p-nitrophenyl palmitate in isopropanol and 9 volumes of 0.2% sodiumdesoxycholate and 0.1% gum arabicum in 50 mM sodiumphosphate buffer pH 8) and 1% carboxymethylcellulose, respectively. After incubation, the absorbance was measured at 410 nm and 575 nm, respectively . A Bradford assay (Coomassie Plus™, Thermo Scientific/Pierce) with BSA as standard was used to determine the total protein concentration in extracts derived from both aerobic and micro-oxic cultures. Antibiotic susceptibility testing was performed on agar plates where bacteria were homogeneously spread over the surface of the agar plate. Antibiotic discs (kanamycin 30 µg, tetracycline 30 µg, gentamycin 10 µg; Alere GmbH) were placed in the center of the plate. Swarming and swimming were tested by inoculating cells onto plates containing ABC medium supplemented with 0.1% casamino acids that were solidified with 0.4% and 0.3% agar, respectively. Plates were incubated for 2 days. Siderophores production was measured on CAS plates as described previously . All phenotypic assays were performed at least in triplicate.
B. cenocepacia was streaked on R2A agar plates and grown overnight at 37°C. From this plate, colonies were picked up and suspended in the GN/GP-IF at the required optical density. The suspensions were then inoculated on Biolog plates PM1 and PM2a for the carbon sources and PM3b for the nitrogen sources (Biolog, Hayward, CA). Plates were incubated at 37°C for 24 h fully aerated or for 36 h under micro-oxic conditions using CampyGen jars (Oxoid, Basingstoke, UK). The optical density was measured using a plate reader; instances where a >50% OD600 difference was observed between micro-oxic and aerobic cells were deemed significant . Each condition was tested in triplicate.
RNA-Seq and data analysis
Total RNA from B. cenocepacia strain H111 grown with 21% or 0.5% oxygen in complex LB medium to the end of the exponential phase (OD600 of 0.8 and 0.4, respectively, Figure 1) was extracted using a modified hot acid phenol protocol . The removal of genomic DNA using DNAseI (Promega) was verified by a PCR reaction with 40 cycles. The samples were then further purified using the RNeasy kit (Qiagen) and the RNA quality was checked using RNA Nano Chips (Agilent 2100 Bioanalyzer; RIN >8). The RNA samples were poly(A)-tailed using poly(A) polymerase. Then, the 5′PPP were removed using tobacco acid pyrophosphatase (TAP). Afterwards, an RNA adapter was ligated to the 5′-monophosphate of the RNA. First-strand cDNA synthesis was performed using an oligo(dT)-adapter primer and the M-MLV reverse transcriptase (Promega). The resulting cDNA was PCR-amplified to about 20–30 ng/µl using a high fidelity DNA polymerase. The cDNA was purified using the Agencourt AMPure XP kit (Beckman Coulter Genomics) and was analyzed by capillary electrophoresis. The primers used for PCR amplification were designed for TruSeq sequencing according to the instructions of Illumina. Illumina single-end sequencing was performed on a HiSeq2000 instrument. The sequence reads were processed and then mapped to the B. cenocepacia H111 genome using CLC Genomics Workbench v4.9 (CLC bio) allowing up to 2 mismatches per read. The mapped reads (or spectral counts, see below) were analyzed using the DESeq software . DESeq models gene/protein expression with a negative binomial distribution and outputs a list of differentially expressed genes/proteins ranked according to statistical significance. We report the top 123 differentially expressed genes (p-value cut-off <0.2), i.e. approx. 2,5% of the genes found actively expressed. This model is more robust against over-identifying candidate regulated genes based on fold-change alone, which can in particular be problematic for genes that are identified with few sequencing reads (common for Burkholderia with their high GC content, ) or spectra. We only considered genes with five or more reads for differential analysis. For functional annotation of H111 genes, we relied on the eggNOG resource  and transferred the functional annotations from the respective J2315 orthologs as described . The RNA-Seq raw data files are accessible through the GEO Series accession number GSE48585.
Preparation of protein samples
Extracellular proteins and subcellular fractions were prepared as described previously . Cells were lysed by two consecutive passes through a French Press homogenizer (Hypramag/Aminco), and cell debris was removed by 15 min centrifugation at 4000 g. Total cell membranes were subsequently harvested by ultracentrifugation for 1 h at 80000 g, 4°C. The pellet containing total membrane proteins was dissolved in 100 mM Tris-HCl, pH 7.5, 2% SDS by incubation at 50°C for 1 h. The cell lysate supernatant containing soluble cytoplasmic proteins was extracted with 6 volumes of ice-cold acetone at −20°C overnight. The precipitated proteins were harvested by centrifugation at 20000 g and dissolved in 100 mM Tris-HCl, pH 7.5, 0.1% SDS. Total protein concentration was determined according to Bradford (Coomassie Plus™ protein assay, Pierce). Approximately 15 mg total protein for each extracellular (EC), cytoplasmic (Cyt) and total membrane (TM) fractions were separated by 1D SDS-PAGE on 12.5% polyacrylamide gels. Gels were stained with colloidal Coomassie Blue (Serva). Individual protein lanes were cut into ten slices and immediately subjected to in-gel tryptic digestion .
Mass spectrometry, protein identification and differential expression analysis
Peptides were separated by RP-HPLC and analyzed by a hybrid LTQ-Orbitrap XL mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) interfaced with a nanoelectrospray source. Mass spectrometric detection was performed in data-dependent mode. Precursor mass spectra were acquired at the Orbitrap mass analyzer with a scan range from m/z 300 to 1,600; resolution was set to 60,000 at m/z 400. Mass spectra were processed with Xcalibur 2.0.7 (Thermo Fisher Scientific) and peak lists were generated with msConvert (version 3.0.4388) . Fragment ion mass spectra were searched with MS-GF+ (MS-GFDB v7747) against a sequence database consisting of 7,258 B. cenocepacia strain H111 proteins (accession CAFQ00000000.1) and 259 common contaminants (e.g. human keratin, trypsin). Spectra were searched for a match to fully-tryptic and semi-tryptic peptides with a mass tolerance of 10ppm. Carbamidomethylation was set as fixed modification for all cysteines while oxidation of methionines, deamidation of asparagines and glutamines as well as cyclization of N-terminal glutamines were considered as optional modifications.
Based on the target-decoy search strategy , a stringent score cutoff was determined that resulted in an estimated FDR of less than 0.2% at the PSM level. PSMs above this cutoff were subjected to a PeptideClassifier analysis  and only peptides that unambiguously identify one protein (either class 1a or 3a) were considered. We furthermore required at least 3 independent spectra or two spectra from two distinct peptides for protein identification. Each subcellular fraction was measured once with a discovery run followed by a subsequent exclusion list run (precursor ions identified in the discovery run were excluded from fragmentation in the exclusion run) . Thereby, about 15% more proteins (272), all preferentially lower abundant, could be added by the exclusion list approach to those identified over all respective first runs (1854, Figure S3). This resulted in a total of 2128 identified proteins at an estimated FDR of less than 1% (0.98%). Total spectral counts for each protein were used for a differential expression analysis with the R package DESeq (version 1.6.1, ). Due to the lower number of spectral counts compared to sequenced reads, we chose a more lenient cut-off of p<0.15 to select the 58 top-ranked differentially expressed proteins for further analysis (roughly 2.7% of all proteins expressed). Protein abundance was estimated according to the method of Schrimpf et al.  (Figure S3). Proteomics data associated with this manuscript can be downloaded from the ProteomeXchange under accession number PXD000270. Signal peptide predictions from SignalP (version 4.0), and transmembrane domain predictions from TMHMM (version 2.0; both from the CBS, Denmark), were used for a combined topology prediction: Proteins without a predicted transmembrane domain after a predicted signal peptide cleavage site are considered secreted. Proteins with one or more predicted transmembrane domains after a predicted signal peptide cleavage site or without a predicted signal peptide cleavage site are assumed to be transmembrane proteins.
Construction and assessment of transcriptional lacZ fusions
For construction of transcriptional lacZ fusions, vector pSU11p  was used. The promoter regions of BCAL2780, BCAM1259, wcaJ genes were first amplified using the primers listed in Table S4 and cloned into vector pCR 2.1 TOPO (Invitrogen, Carlsbad, CA). After sequence verification, the promoter probes were cut and cloned into pSU11p using HindIII and XhoI. The resulting plasmids pPBCAL2780-lacZ, pPBCAM1259-lacZ and pPwcaJ-lacZ were transferred by triparental mating into B. cenocepacia strain H111 and ß-galactosidase activity was determined both under micro-oxic and aerobic conditions by the Miller method . Briefly, the strains were grown overnight in LB broth, then subcultured in LB medium and incubated for 2 days (aerobic cultures) or 4 days (micro-oxic cultures). The experiment was run in triplicate. ß-galactosidase activity was also visually inspected on LB plates containing 5-bromo-4-chloro-3-indolyl-β-D-galactoside(X-Gal) (Sigma). Bacterial strains, plasmid and primers used in this study are listed in Table S4.
The expression of H111 orthologs of J2315 genes BCAM1259, BCAL0785, BCAL1919, BCAM1010, BCAM0049 and BCAL2118 was analyzed with a Mx3000P instrument using Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent, Switzerland) and cDNA prepared from biological replicates as template. Each reaction contained 12.5 µl 2× Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix, 0.7 µM of individual primers and 15 or 7.5 or 3.5 ng of cDNA in a total volume of 25 µl. Reactions were run in triplicates. The relative expression ratio was calculated according to Pfaffl  using the primary sigma factor rpoD (BCAM0918) as housekeeping gene. The primers used are listed in Table S4.
Continuous normally distributed data were analyzed by using an independent sample t-test. P-values were determined using SPSS software, version 21.0. The over-representation analysis of EggNOG functional categories was carried out using Fisher's Exact tests.
Decreased siderophore production in micro-oxic conditions. Siderophore production of B. cenocepacia H111 grown under aerobic (black bar) and micro-oxic (grey bar) conditions was measured on CAS plates. The measured halo diameter corresponds to siderophore activity. Whiskers indicate SD, n = 3.
Validation of four micro-oxic induced genes by lacZ fusions. The activity of BCAL2780 (thioredoxin domain containing protein), BCAM1259 (sigma factor), wcaJ (CCE50896, sugar transferase in cepacian cluster II), bclA (lectin) and cepI promoter fusion was determined in the wild type grown in aerobic (black bar) and micro-oxic (grey bar) conditions. Whiskers indicate SD, n = 3.
Proteins identified by the exclusion list approach add 272 preferentially low abundant proteins. The exclusion list approach was successful in adding preferentially lower abundant proteins (red curve) on top of those identified over all discovery runs (blue curve) and allowed us to dig deeper into the proteome. For calculation of the relative protein abundance, see Methods.
Shotgun proteomics and RNA-Seq data for all B. cenocepacia H111 genes/proteins grown in micro-oxic (M) conditions and aerobic (A) conditions.
List of 55 B. cenocepacia H111 and J2315 genes that are commonly induced by low oxygen (M) (DESeq analysis, p-value<0.2 for H111, fold change >2 for J2315). The expression in aerobic cells was taken as baseline (A).
Q-PCR results for selected genes.
Bacterial strains, plasmids and oligonucleotides used in this study.
We gratefully acknowledge Cynthia Sharma and Konrad Förstner (University of Würzburg) for processing our RNA-Seq samples. We thank Martina Lardi for help in statistical analysis and Kirsty Agnoli for assistance in processing Biolog plates. Hans-Martin Fischer is acknowledged for providing access to the gas station at the Microbiology Institute of the ETH Zurich.
Conceived and designed the experiments: GP CHA LE. Performed the experiments: GP RB AG. Analyzed the data: GP RB AG UO CHA LE. Wrote the paper: GP LE.
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