Progressive retinal degenerations are the most common causes of complete blindness both in human and in dogs. Canine progressive retinal atrophy (PRA) or degeneration resembles human retinitis pigmentosa (RP) and is characterized by a progressive loss of rod photoreceptor cells followed by a loss of cone function. The primary clinical signs are detected as vision impairment in a dim light. Although several genes have been associated with PRAs, there are still PRAs of unknown genetic cause in many breeds, including Papillons and Phalènes. We have performed a genome wide association and linkage studies in cohort of 6 affected Papillons and Phalènes and 14 healthy control dogs to map a novel PRA locus on canine chromosome 2, with a 1.9 Mb shared homozygous region in the affected dogs. Parallel exome sequencing of a trio identified an indel mutation, including a 1-bp deletion, followed by a 6-bp insertion in the CNGB1 gene. This mutation causes a frameshift and premature stop codon leading to probable nonsense mediated decay (NMD) of the CNGB1 mRNA. The mutation segregated with the disease and was confirmed in a larger cohort of 145 Papillons and Phalènes (PFisher = 1.4×10−8) with a carrier frequency of 17.2 %. This breed specific mutation was not present in 334 healthy dogs from 10 other breeds or 121 PRA affected dogs from 44 other breeds. CNGB1 is important for the photoreceptor cell function its defects have been previously associated with retinal degeneration in both human and mouse. Our study indicates that a frameshift mutation in CNGB1 is a cause of PRA in Papillons and Phalènes and establishes the breed as a large functional animal model for further characterization of retinal CNGB1 biology and possible retinal gene therapy trials. This study enables also the development of a genetic test for breeding purposes.
Citation: Ahonen SJ, Arumilli M, Lohi H (2013) A CNGB1 Frameshift Mutation in Papillon and Phalène Dogs with Progressive Retinal Atrophy. PLoS ONE 8(8): e72122. doi:10.1371/journal.pone.0072122
Editor: Ralf Krahe, University of Texas MD Anderson Cancer Center, United States of America
Received: May 16, 2013; Accepted: July 10, 2013; Published: August 28, 2013
Copyright: © 2013 Ahonen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This study was supported partly by the Academy of Finland, the Sigrid Juselius Foundation, Biocentrum Helsinki, the Jane and Aatos Erkko Foundation. SA is a student in Helsinki Graduate Program in Biotechnology and Molecular Biology. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Progressive retinal degeneration or atrophy is one of the most common causes of blindness both in human and in dog affecting the retinal photoreceptor cells. The most typical type of canine progressive retinal atrophy (PRA) is primary rod degeneration, where the light sensitive rod photoreceptor cells degenerate, resulting visual impairment in the dark light. When the disease progresses to the cone cells, which function in the day light, the vision is completely lost . Ophthalmoscopically the initial clinical signs include tapetal hyperreflectivity, retinal vascular attenuation and pigment changes and atrophy of the optic nerve. 
Various forms of PRA have been diagnosed in different breeds of dog, some breeds have been found to manifest more than one type of the disease. Most canine PRAs are recessively inherited and 12 causative genes are known including ADAM metallopeptidase domain 9 (ADAM9) , bestrophin (BEST1) , chromosome 2 open reading frame 71 (C2orf71) , cyclic nucleotide gated channel beta 3 (CNGB3) , nephronophthisis (NPHP4) , phosphodiesterase 6A, cGMP-specific, rod, alpha (PDE6A) , rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta (PDE6B) , rhodopsin (RHO) , retinitis pigmentosa GTPase regulator-interacting protein (RPGRIP1) , retinal pigment epithelium-specific protein (RPE65) , solute carrier family 4, anion exchanger, member 3 (SLC4A3) , progressive rod-cone degeneration (PRCD) gene . Furthermore, three X-linked canine PRAs are known , . However, there are still other PRA affected breeds of unknown genetic cause.
Papillon breed is affected with an autosomal recessive late onset PRA with a mean onset at 5.6 years of age . Based on the electroretinogram (ERG) recordings, affected dogs have a primary loss of the rod photoreceptor cells, followed by loss of cone cell function , . The first clinical signs are seen as difficulties in the dim light. The disease progress very slowly and the affected dogs seem to be visually normal throughout their life, as the cone function is fairly well preserved , . The ophthalmoscopical signs include increased tapetal reflectivity and retinal vascular attenuation followed by pigment migration in the non-tapetal fundus .
According to Fèdèration Cynologique Internationale (FCI), Papillon breed is separated to two different breeds, Papillon and Phalène in Europe. Separation is based on the ear morphology, Papillons having bricked ears and Phalènes more low set ears. Genetic background is expected to be similar as both types of dogs are born in the same litter and puppies are registered in Europe based on their ear morphology. The American Kennel Club registers both types as Papillons. Due to shared breed origin, it is reasonable to hypothesize that the genetic cause of PRA is shared.
We have established a pedigree and a study cohort of PRA affected and unaffected Papillons and Phalènes to identify the cause of the disease. We performed a genome wide association, linkage and exome sequencing studies to map the disease to a region in CFA2 and to identify a frameshift-causing indel mutation in the cyclic nucleotide gated channel beta 1 (CNGB1) gene in the affected dogs.
Materials and Methods
Blood and buccal swab samples were collected from pet dogs to the Dog DNA bank at the University of Helsinki, Finland. All samples were submitted by the owners’ consent and were collected under the permission of animal ethical committee of County Administrative Board of Southern Finland (ESAVI/6054/04.10.03/2012). Genomic DNA was extracted from EDTA blood samples, using Chemagic Magnetic Separation Module I (MSM I) (Chemagen Biopolymer-Technologie AG, Baeswieler, Germany) according to the manufacturer’s instructions. DNA from buccal swabs (Eurotubo Cytobrush, sterile, 200mm, Danlab, Helsinki, Finland) was extracted using QIAamp DNA Mini Kit (Qiagen). A retinal sample from an Australian Cattle Dog, euthanized for unrelated causes and free of PRA, was taken post mortem with owner’s consent. RNA was extracted using RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions. DNA and RNA concentrations were measured using Nanodrop ND-1000 UV/Vis Spectrophotometer (Nanodrop technologies, Wilmington, Delaware, USA) and stored at -20°C (DNA) or −80°C (RNA).
We have collected altogether samples from 59 Papillons and from 116 Phalènes in our Dog DNA bank. We selected clinically confirmed cases for our genetic studies. Samples were collected from affected Papillons (n = 4) or Phalènes (n = 2) with clinical signs consistent with PRA, including tapetal hyper-reflectivity, retinal pigmentary changes and vascular attenuation. Control dogs (n = 14) in the mapping study were eye examined healthy over seven years of age.
A pedigree was constructed around the affected dogs using the genealogical data available in public canine registries such as the Finnish Kennel Club’s Koiranet, the Swedish Kennel Club’s Hunddata databases or as informed by the owners. A GenoPro genealogy software was utilized to draw the pedigree.
Genome wide association study
A genome-wide association study (GWAS) was performed using Illumina’s CanineHD BeadChip arrays (San Diego, CA, USA) for 6 cases (4 Papillons, 2 Phalènes) and 14 controls (3 Papillons, 11 Phalènes). Genotyping was performed in our core facility at the FIMM Technology Center. Quality control procedures were included when analyzing the data. Only SNPs which met Hardy-Weinberg expectations P≤0.0001, had ≥95% genotyping rate and minor allele frequency (MAF) of 5% were included in the analysis, resulting in the exclusion of 64640 SNPs out of 173662. The GWAS data is available from the researchers upon request.
To compare the allele frequencies, a case-control association test was performed using PLINK 1.07 . Significance values from these analyses were used to generate a whole-genome association plot using the statistical package R . Identity-by-state (IBS) clustering and CMH meta-analysis (PLINK) were used to adjust for population stratification. Genome-wide corrected empirical p-values were determined by applying 100.000 permutations to the data. The GWAS data was also re-analyzed (PLINK) by adjusting for the strongest SNP on CFA2 (BICF2P309315) to detect the presence of any modifier loci.
Besides association, the genotyping data was analyzed using a joint family-based linkage and association analysis program Pseudomarker . The family-based analyses were performed under a recessive inheritance model, and included parametric single-point linkage test, association analysis (LD|Linkage) and joint analysis (LD+Linkage).
Exome sequencing was performed for a trio of Phalènes, including an affected proband and healthy parent dogs (Fig. 1). The coding sequences were captured using Agilent’s Canine Exome Capture Kit (Agilent, Santa Clara, CA, USA) that was designed based on the build 2.1 of the canine genome reference sequence. The capture included a 54 Mb design that covered the Ensemble and RefSeq Genes from the UCSC track, human protein alignments and spliced ESTs that lie outside of Ensemble. The capture was performed according the manufacturer’s instruction and the sequencing was performed using the Illumina HiSeq2000. Both the capture and sequencing were performed at the FIMM technology center. The exome sequencing data is available upon request.
Figure 1. Pedigree of PRA affected Papillons and Phalènes.
Pedigree indicates the affected dogs that were used in the study. Samples from six affected dogs were available for genotyping. Disease segregation is consistent with autosomal recessive mode of inheritance as all affected dogs are born from healthy parents and both sexes are affected. Obligate carrier parents of affected dogs are marked in the pedigree. Obligate carriers genotyped as heterozygous for CNGB1 mutation are marked with a yellow background.doi:10.1371/journal.pone.0072122.g001
The data analysis included quality control, alignment, variant calling and annotation of the variants. Quality control was performed using FASTX toolkit (http://hannonlab.cshl.edu/fastx_toolkit/index.html) to remove the low quality raw-data and to reduce the false positives, followed by alignment of the reads to the build 2.1 of the canine genome reference sequence with Burrows-Wheeler (BWA) aligner tool . The aligned reads were then directed to the variant calling programs GATK  and Samtools  to identify the SNPs and short indels present within the exome samples. Pindel  program was used to identify the structural variants. Finally, the identified variants were annotated against NCBI and Ensembl databases to find out the variants present in the coding and non-coding regions and to the dbSNP131 to identify known polymorphic variants. The annotation was performed using ANNOVAR  and in-house custom R-scripts. The data was then filtered assuming a recessive mode of inheritance using in-house R-scripts.
Mutation screening and validation
An indel in exon 25, including 1-bp deletion followed by 6-bp insertion was found in the cyclic nucleotide gated channel beta 1 (CNGB1) and a non-synonymous variant in 2-oxoglutarate and iron-dependent oxygenase domain containing 1 (OGFOD1) genes. These variants were confirmed by PCR in additional cases and controls. PCR primer pairs were designed using Primer3  software to amplify the variants from genomic DNA. The mutation on exon 25 of the CNGB1 gene was amplified from 6 PRA affected and 14 control Papillons and Phalènes and one Rough Collie using forward primer 5'-AACAATCTCCTGGAGCCTCA-3' and reverse primer 5'-TGGAAGCAACGGTAGAGAAGA-3'. The non-synonymous mutation on exon 9 of the OGFOD1 gene was amplified from 6 PRA affected and 10 controls dogs and one Rough Collie with forward primer 5'-CTTGTGCTTGAGTTGGATGTG-3' and reverse primer 5'-TGGGAGAGCGAAGACTTTGT-3'.
The best associated SNP on CFA1 (BICF2P839236) was amplified in a larger sample cohort of 6 affected and 168 controls using forward primer 5'-TCCAATCCTTAATGTTCTAAAGTGAA-3' and reverse primer 5'-CCCAGATTAAGATGATTCACCA-3'.
To further investigate the disease associated variant in a larger sample cohort the CNGB1 variant was sequenced from 43 Papillons and 93 Phalènes. In addition, the mutation was studied in 121 dogs from other breeds affected with PRA or retinal degeneration, representing 44 breeds (Table S3) and 334 healthy control dogs from 10 breeds, including Australian Shepherd (n = 20), Border Collie (n = 92), Chihuahua (n = 19), Finnish Lapphund (n = 93), Japanese Chin (n = 14), Shetland Sheepdog (n = 20), Silk Terrier (n = 20), Toy Poodle (n = 20), West Highland White Terrier (n = 20) and Wire Haired Dachshund (n = 16).
The PCR reactions were carried in 12 μl reactions consisting of 0.6 U Biotools polymerase (Biotools, Madrid, Spain), 200 μM dNTPs (Thermo Scientific), 1.5 mM MgCl2 (Biotools), 1 x PCR buffer (Biotools), 0.83μl of forward and reverse primers (Sigma Aldrich) and 10 ng template genomic DNA or mRNA. The reaction mixtures were subjected to a thermal cycling program of 95°C for 10 min, followed by 35 cycles of 95°C for 30 s, 30 s at the annealing temperature 60°C, and 72°C for 60 s and final elongation stage of 72°C for 10 min. The PCR products were purified using ExoSap (Thermo Scientific) according to manufacturer’s instructions and sequenced using the Sanger sequencing. Sequence analysis was performed using Sequencher software (Gene Codes, Ann Arnbor, MI, USA).
The CNGB1 mRNA sequence (XM_848817.1) was amplified using Biotools (Biotools) polymerase and PCR protocol described above with annealing temperature of 62°C. The primers used for amplification are listed in supplementary data (Table S1).
We established a cohort of PRA affected and unaffected Papillons and Phalènes to map the disease locus and to identify the genetic cause. Clinical signs reported from the affected dogs were consistent with PRA, including tapetal hyper-reflectivity and vascular attenuation. The average age of the PRA diagnosis was 4.3 years. The pedigree established around the affected dogs suggested a recessive mode of inheritance (Fig. 1).
We first studied the retinitis pigmentosa GTPase regulator interacting protein 1 (RPGRIP1), originally associated with PRA in the Miniature Longhaired Dachshunds as a candidate gene in our study cohort using the described PCR conditions for the mutation . We found a high carrier frequency in the breed 17.1 % (25/146), but only one affected dog was homozygous for RPGRIP1 mutation. This leaves the role of RPGRIP1 unclear and we, therefore, initiated a genome-wide association and linkage analyses to map the PRA locus in a cohort of 6 PRA affected and 14 unaffected dogs. Control dogs were ophthalmologically normal when examined at greater than seven years of age. Statistical analysis by PLINK  identified the most highly associated SNP, BICF2P309315 in CFA2 at 61428709 bp (praw = 4.7×10−6, pgenome = 0.1) (build 2.1 of the canine genome reference sequence) (Fig. 2A). The association was supported by six closely positioned markers. Another tentative region with two separate SNPs positioned from 70352612 bp to 77628107 bp was found in CFA1 with the best associated SNP, BICF2P839236 (praw = 7.0×10−6, pgenome = 0.2). Re-analysis of the data by adjusting for the strongest SNP (BICF2P309315) on CFA2 did not support the association on CFA1 (BICF2P839236, p = 0.9) confirming the association on CFA2. In addition, genotyping of the best associated SNP on CFA1 in a larger cohort of 6 cases and 168 controls (p = 6.97×10−5) did not improve the original tentative GWAS association (p = 10−6) and strongly suggest that CFA1 does not play a role in the disease etiology. No significant population structure was identified by genome wide IBS clustering (genomic inflation factor = 1).
Figure 2. Genome wide association and linkage analyses.
A) A Manhattan plot of genome-wide case-control association analysis performed using 6 cases and 14 controls indicate the most highly associated region in CFA2. B) The PRA associated region on chromosome 2 spans from 61.4 Mb to 63.3 Mb based on association, linkage and joint analyses. C) Genotypes at the PRA associated region on CFA2. All cases share a 1.9 Mb homozygous block, and within this block SNPs BICF2S23238410, BICF2P309315 and BICF2P75954 show complete recessive segregation with the disease. D) Chromatograms of the c.2685delA (arrow), c.2687_2688insTAGCTA (shadowed) mutations in CNGB1 gene in an affected (2) and normal (1) dog. The CNGB1 gene is located between the two segregating SNPs (BICF2P309315, BICF2P75954).doi:10.1371/journal.pone.0072122.g002
The association results were confirmed by a joint family-based linkage and association analysis using a Pseudomarker program. The joint analysis identified the most highly linked region covering nine SNPs at the same locus on CFA2 between 61198715 bp to 64929333 bp, (linkage p = 1.0×10−6, association p = 8.7×10−6 and joint analysis p = 1.9×10−7) (Fig. 2B). A weaker association was identified also in CFA1 (linkage p = 1.0×10−6, association p = 2.0×10−6 and joint analysis p = 2.0×10−6) (data not shown).
Assessment of genotypes at the associated CFA2 locus revealed a shared 1.9 Mb homozygous haplotype block in all of the affected dogs (Fig. 2C), with recessively segregating markers between 61420765-61645929 bp. The associated region contains 37 genes, including a known human RP gene, CNGB1.
Based on the exome sequencing data, on average 97 % of the generated raw reads were aligned to CanFam2.1 reference sequence per individual. Of these reads 76 % on average, were aligned to the target region. Each individual had a mean depth of 95 reads on average in the targeted region and 98.9 % of the targeted region was covered with at least one read and about 74 % of the target regions were covered with at least 20 reads (Table S2). The variant calling algorithms detected altogether 129590 single nucleotide variants (SNVs) and 25905 indels across the three individuals. After analyzing the trio according to a recessive mode of inheritance (an affected proband and healthy parent dogs), 204 SNVs and 9 indels were identified. Of the coding variants, two were located in the associated region on CFA2: a 1-bp deletion followed by a 6-bp insertion in the CNGB1 gene (c.2685delA2687_2688insTAGCTA) (Fig. 2D) and a non-synonymous c.1128G>A, p.376M>I mutation in the OGFOD1 gene. The indel variant in CNGB1 causes a frameshift and a premature stop-codon p.Tyr889Serfs*5 in an evolutionary conserved region (Fig. 3A and B), while the missense mutation in OGFOD1 was predicted to be benign based on the bioinformatics prediction using Polyphen2  and SIFT-programs . We further analyzed the exome data for the region on CFA1, but did not have any case-specific variants, supporting the CFA2 as the causative locus and CNGB1 mutation as causative for the PRA in the breeds.
Figure 3. CNGB1 protein alignments.
A) CNGB1 amino acid alignment of the normal and affected dogs. The p.Tyr889Serfs*5 mutation in the affected dog results in a loss of a significant part of the C-terminus of the protein and probable NMD of the CNGB1 mRNA B) CNGB1 sequence alignment between different vertebrates. The mutation is located in a highly conserved region across species. The arrows mark the first mutated amino acid caused by the frameshift and the premature stop codon.doi:10.1371/journal.pone.0072122.g003
To gather further evidence for the CNGB1 mutation, we genotyped all six PRA affected dogs and all were homozygous indicating a full segregation with the disease. We also genotyped four obligatory carriers from the pedigree (Fig. 1) and they were all mutation carriers. We then genotyped additional 145 randomly selected unaffected Papillons and Phalènes and found four additional dogs homozygous for the mutation and a 17.2% carrier frequency (25/145) was indicated. Of the four homozygous dogs found in the population screening, one was reported to have vision problems in the dim light, which may indicate that the dog is indeed affected with PRA. However, the dog has not had ophthalmologic examination. This dog’s full sibling was also homozygous but neither has been ophthalmologically examined. Two other homozygous dogs had been ophthalmologically examined at very young age (1-2 years), well before the average age of onset of the PRA in the breed and it is likely that the clinical signs are not present at this age. The clinical follow up of these dogs would be important in coming years. We found also 25 carriers of which 52 % have been ophthalmologically examined after 4 years of age and none of them have been diagnosed with PRA. These results indicate a highly significant association between the mutation and disease (PFisher = 1.4×10−8) when comparing genotyped homozygous affected dogs (n = 6) and dogs free of PRA using Fisher’s exact test. The healthy dogs included 4 dogs homozygous for the mutation, 25 heterozygous and 116 dog homozygous for the reference allele.
To gain further support for the causality of the CNGB1 mutation, we screened additional 334 healthy dogs from 10 other breeds but the mutation was not found. In addition, the mutation was studied in 121 dogs from 44 other breeds affected with PRA or retinal degeneration (Table S3), but none of these affected dogs had the CNGB1 mutation. Collectively, these results indicate that the CNGB1 mutation is causal and breed-specific in Papillons and Phalènes.
The entire CNGB1 mRNA was successfully amplified from a retinal sample from a PRA-free Australian Cattle Dog [Genbank Accession KF551910], indicating that the canine CNGB1 is also transcribed in the retina.
We have performed a series of genetic analyses, including GWAS, linkage and exome sequencing to identify the mutation for PRA in Papillon and Phalène breeds. Although we managed to establish only a small cohort and pedigree of cases and controls, we mapped a 1.9 Mb locus at CFA2, and confirmed it by both association and linkage approaches according to our pedigree analyses with the expected recessive mode of inheritance. The associated region at CFA2 contains 37 genes, including a known human retinitis pigmentosa gene, CNGB1. It is clinically and functionally highly relevant candidate gene for PRA in the Papillon and Phalène breeds. Importantly, our exome data, covering all the coding regions of the associated locus, revealed CNGB1 to be the only gene with a disease segregating pathogenic mutation: a complex indel variant was found in exon 25 resulting in a premature stop codon and predicting nonsense mediated decay (NMD) of the CNGB1 mRNA. NMD eliminates the production of mRNA that contains premature translation termination codon and code for nonfunctional polypeptide. In mammalian cells nonsense codons are recognized after splicing and mRNA is programmed to NMD, if there is at least one splicing generated exon-exon junction >50-55 nucleotides downstream of the premature termination codon , . As the identified mutation in the CNGB1 is on exon 25 and the c.2685delA2687_2688insTAGCTA variation is 127 nucleotides from the nearest exon-exon junction downstream, it is hypothesized that the mRNA is subjected to NMD and no protein is translated.
The CNGB1 gene encodes for a rod photoreceptor cyclic guanosine monophosphate-gated (cGMP) channel β-subunit and is a part of the rod photoreceptor cGMP-gated cation channel complex . This complex consists α and β-subunits and form tetrameric cyclic nucleotide gated (CNG) channels , . The visual transduction cascade is mediated by this non-selective cation channel, which is directly gated by cGMP . The channel controls for the flow of sodium and calcium ions through the plasma membrane in response to light induced changes in cGMP level . The β-subunit contains a channel-like structure, but does not form functional subunit without the co-expression of the α-subunit , . The α-subunit contains six membrane segments, a voltage sensor region, a pore region and a cGMP-binding motif .
The human CNGB1 comprises of 33 exons and several different transcripts have been found in different tissues . In the retina, at least seven different transcripts are expressed . Rod cells contain a long isoform of the CNGB1 (CNGB1a) . The shorter isoform (CNGB1b) is expressed in the olfactory CNG channels  as well as sperm cells and other tissues . The N-terminal part of CNGB1 between exons 1-16 is a glutamic acid-rich and it is also expressed as a separate protein (GARP) . GARP is highly enriched in rod photoreceptors . The C-terminal part is homologous to the α-subunit and forms the channel-like portion. Exons 21-26 are homologous to the transmembrane and pore domains present in the α-subunit, exons 29-31 encodes for the cyclic nucleotide binding domain (CNBD), and exons 19 and 32 codes for domain that are important for the calcium calmodulin/regulation of cGMP affinity .
The canine CNGB1 is similar to human and contains 32 predicted exons based on mRNA (XM_848817.1) sequence alignment. The frameshift mutation in exon 25 predicts that the CNGB1 mRNA is destroyed by NMD. Therefore, although we lack functional data due to limited access to retinal tissues from the affected dogs, it is likely that the mutation abrogates CNGB1 functions and causes retinal degeneration in Papillons and Phalènes. These breeds provide a model to study the genotype-phenotype correlations related to the possible alternative transcripts and their potential tissue-specific functions.
The defective CNGB1 has been implicated as a cause of retinal degeneration in human and mouse. Bareil et al. identified a p.G993V missense mutation is a consanguineous French family affected by a night blindness and progressive loss of peripheral vision and ERG recorded rod responses . In another study, a splice site point mutation (c.3444+1G>A) was found in a patient with night blindness, retinal pigment lesion and non-recordable dark adapted flash ERG . In addition, a missense mutation (c.2957A→T; p.N986I) was found in a patient with typical RP with attenuation of the retinal vessels, retinal pigment atrophy and optic disc pallor .
In the CNGB1−/− mice, the CNGB1 gene was found to be important for the formation and outer segment targeting of the rod CNG channels. The CNGB1-deficient mice developed retinal degeneration that resembles human RP, progressing from the rod degeneration to a complete loss of rod function and secondary degeneration of the cones . However, the disease in CNGB1−/− mice progresses more slowly when compared to other RP mouse models and the exact events that lead to rod cell death are unclear . In CNGB1−/− mice, 80−90% loss of the rod responses is not detected until at the age 1 year . In human patients, night blindness is usually reported at school age but RP is commonly diagnosed around 30 years of age ,  The PRA in Papillon and Phaléne breeds is diagnosed at the middle age dogs and progresses similar to human and mice.
In summary, we have discovered a putative genetic cause of PRA in the Phalène and Papillon breeds by identifying a frameshift mutation in CNGB1. As a further confirmation, the same mutation was also recently reported in an independent study by Petersen-Jones et al. (2013) at the Association for Research in Vision and Ophthalmology (ARVO) conference (unpublished data). Our study enables the development of a genetic test to eradicate the disease from the breeds. The breeds have a high carrier frequency (17.2 %) and careful breeding plans by mating carriers to normal should be considered to maintain genetic diversity. Importantly, our study establishes a large functional knockout animal model for potential therapeutic trials such as gene therapy, and provides a relevant model for further studies of the CNGB1 biology in the retina and other tissues.
Primers used for PCR amplification and sequencing of canine CNGB1 mRNA.
Summary of the exome sequencing data analysis of each sequenced individual dog.
Dogs affected with PRA (44 breeds, total 121) were sequenced for the CNGB1 mutation.
We would like to thank all owners that donated samples to our DNA-bank from their dogs. The Finnish Papillon and Phalène Club is thanked for their co-operation. Minna Virta, Ranja Eklund, Sini Karjalainen, Heta Haikonen and Elina Vuorenmaa are thanked for their technical support and help in sample recruiting and pedigree analyses.
Conceived and designed the experiments: SA. Performed the experiments: SA MA. Analyzed the data: SA MA. Contributed reagents/materials/analysis tools: SA MA. Wrote the paper: SA HL.
- 1. Parry H (1953) Degenerations of the dog retina: II. generalized progressive atrophy of hereditary origin. Br J Ophthalmol 37: 487. doi: 10.1136/bjo.37.8.487
- 2. Petersen-Jones SM (1998) Animal models of human retinal dystrophies. Eye 12: 566–570. doi: 10.1038/eye.1998.146
- 3. Goldstein O, Mezey JG, Boyko AR, Gao C, Wang W, et al. (2010) An ADAM9 mutation in canine cone-rod dystrophy 3 establishes homology with human cone-rod dystrophy 9. Molecular vision 16: 1549.
- 4. Guziewicz KE, Zangerl B, Lindauer SJ, Mullins RF, Sandmeyer LS, et al. (2007) Bestrophin gene mutations cause canine multifocal retinopathy: A novel animal model for best disease. Invest Ophthalmol Vis Sci 48: 1959–1967. doi: 10.1167/iovs.06-1374
- 5. Downs L, Bell J, Freeman J, Hartley C, Hayward L, et al. (2012) Late-onset progressive retinal atrophy in the Gordon and Irish Setter breeds is associated with a frameshift mutation in C2orf71. Anim Genet 44: 169–77. doi: 10.1111/j.1365-2052.2012.02379.x
- 6. Sidjanin DJ, Lowe JK, McElwee JL, Milne BS, Phippen TM, et al. (2002) Canine CNGB3 mutations establish cone degeneration as orthologous to the human achromatopsia locus ACHM3. Hum Mol Genet 11: 1823. doi: 10.1093/hmg/11.16.1823
- 7. Wiik AC, Wade C, Biagi T, Ropstad EO, Bjerkås E, et al. (2008) A deletion in nephronophthisis 4 (NPHP4) is associated with recessive cone-rod dystrophy in Standard Wire-Haired Dachshund. Genome Res 18: 1415. doi: 10.1101/gr.074302.107
- 8. Petersen–Jones SM, Entz DD, Sargan DR (1999) cGMP phosphodiesterase-α mutation causes progressive retinal atrophy in the Cardigan Welsh Corgi dog. Invest Ophthalmol Vis Sci 40: 1637.
- 9. Dekomien G, Runte M, Gödde R, Epplen J (2000) Generalized progressive retinal atrophy of Sloughi dogs is due to an 8-bp insertion in exon 21 of the PDE6B gene. Cytogenetic and Genome Research 90: 261–267.
- 10. Kijas JW, Cideciyan AV, Aleman TS, Pianta MJ, Pearce-Kelling SE, et al. (2002) Naturally occurring rhodopsin mutation in the dog causes retinal dysfunction and degeneration mimicking human dominant retinitis pigmentosa. Proceedings of the National Academy of Sciences 99: 6328.
- 11. Mellersh C, Boursnell M, Pettitt L, Ryder E, Holmes N, et al. (2006) Canine RPGRIP mutation establishes cone–rod dystrophy in Miniature Longhaired Dachshunds as a homologue of human Leber Congenital Amaurosis. Genomics 88: 293–301. doi: 10.1016/j.ygeno.2006.05.004
- 12. Aguirre GD, Baldwin V, Pearce-Kelling S, Ray K, Acland GM (1998) Congenital stationary night blindness in the dog: Common mutation in the RPE65 gene indicates founder effect. Mol Vis 4: 23.
- 13. Downs LM, Wallin-Håkansson B, Boursnell M, Marklund S, Hedhammar Å, et al. (2011) A frameshift mutation in Golden Retriever dogs with progressive retinal atrophy endorses SLC4A3 as a candidate gene for human retinal degenerations. PloS one 6: e21452. doi: 10.1371/journal.pone.0021452
- 14. Zangerl B, Goldstein O, Philp AR, Lindauer SJ, Pearce-Kelling SE, et al. (2006) Identical mutation in a novel retinal gene causes progressive rod-cone degeneration in dogs and retinitis pigmentosa in humans. Genomics 88: 551–563. doi: 10.1016/j.ygeno.2006.07.007
- 15. Zhang Q, Acland GM, Wu WX, Johnson JL, Pearce-Kelling S, et al. (2002) Different RPGR exon ORF15 mutations in canids provide insights into photoreceptor cell degeneration. Hum Mol Genet 11: 993–1003. doi: 10.1093/hmg/11.9.993
- 16. Vilboux T, Chaudieu G, Jeannin P, Delattre D, Hedan B, et al. (2008) Progressive retinal atrophy in the Border Collie: A new XLPRA. BMC veterinary research 4: 10. doi: 10.1186/1746-6148-4-10
- 17. Hakanson N, Narfstrom K (1995) Progressive retinal atrophy in Papillon dogs in Sweden: A clinical survey. Veterinary and comparative ophthalmology 5: 83–87.
- 18. Narfstrom K, Ekesten B (1998) Electroretinographic evaluation of Papillons with and without hereditary retinal degeneration. Am J Vet Res 59: 221–226.
- 19. Narfstrom K, Wrigstad A (1999) Clinical, electrophysiological and morphological changes in a case of hereditary retinal degeneration in the Papillon dog. Vet Ophthalmol 2: 67–74.
- 20. Purcell S, Neale B, Todd-Brown K, Thomas L, Ferreira MA, et al. (2007) PLINK: A tool set for whole-genome association and population-based linkage analyses. Am J Hum Genet 81: 559–575. doi: 10.1086/519795
- 21. R Development Core Team, R foundation for Statistical Computing (2009) R: A language and enviroment for statistical computing.
- 22. Hiekkalinna T, Schäffer AA, Lambert B, Norrgrann P, Göring HH, et al. (2011) PSEUDOMARKER: A powerful program for joint linkage and/or linkage disequilibrium analysis on mixtures of singletons and related individuals. Hum Hered 71: 256–266. doi: 10.1159/000329467
- 23. Li H, Durbin R (2010) Fast and accurate long-read alignment with Burrows–Wheeler transform. Bioinformatics 26: 589–595. doi: 10.1093/bioinformatics/btp698
- 24. McKenna A, Hanna M, Banks E, Sivachenko A, Cibulskis K, et al. (2010) The genome analysis toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data. Genome Res 20: 1297–1303. doi: 10.1101/gr.107524.110
- 25. Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, et al. (2009) The sequence alignment/map format and SAMtools. Bioinformatics 25: 2078–2079. doi: 10.1093/bioinformatics/btp352
- 26. Ye K, Schulz MH, Long Q, Apweiler R, Ning Z (2009) Pindel: A pattern growth approach to detect break points of large deletions and medium sized insertions from paired-end short reads. Bioinformatics 25: 2865–2871. doi: 10.1093/bioinformatics/btp394
- 27. Wang K, Li M, Hakonarson H (2010) ANNOVAR: Functional annotation of genetic variants from high-throughput sequencing data. Nucleic Acids Res 38: e164–e164. doi: 10.1093/nar/gkq603
- 28. Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132: 365–386.
- 29. Adzhubei IA, Schmidt S, Peshkin L, Ramensky VE, Gerasimova A, et al. (2010) A method and server for predicting damaging missense mutations. Nature methods 7: 248–249. doi: 10.1038/nmeth0410-248
- 30. Ng PC, Henikoff S (2001) Predicting deleterious amino acid substitutions. Genome Res 11: 863–874. doi: 10.1101/gr.176601
- 31. Nagy E, Maquat LE (1998) A rule for termination-codon position within intron-containing genes: when nonsense affects RNA abundance. Trends Biochem Sci 23: 198–199. doi: 10.1016/S0968-0004(98)01208-0
- 32. Lejeune F, Maquat LE (2005) Mechanistic links between nonsense-mediated mRNA decay and pre-mRNA splicing in mammalian cells. Current Opinion in Cell Biology 17: 309–315. doi: 10.1016/j.ceb.2005.03.002
- 33. Körschen HG, Illing M, Seifer R, Sesti F, Williams A, et al. (1995) A 240 kDa protein represents the complete β subunit of the cyclic nucleotide-gated channel from rod photoreceptor. Neuron 15: 627–636. doi: 10.1016/0896-6273(95)90151-5
- 34. Chen T, Peng Y, Dhallan R, Ahamed B, Reed R, et al. (1993) A new subunit of the cyclic nucleotide-gated cation channel in retinal rods. Nature 362: 764–767. doi: 10.1038/362764a0
- 35. Hofmann F, Biel M, Kaupp UB (2003) International union of pharmacology. XLII. compendium of voltage-gated ion channels: Cyclic nucleotide-modulated channels. Pharmacol Rev 55: 587–589. doi: 10.1124/pr.55.4.10
- 36. Yau K (1994) Cyclic nucleotide-gated channels: An expanding new family of ion channels. Proc Natl Acad Sci U S A 91: 3481. doi: 10.1073/pnas.91.9.3481
- 37. Heginbotham L, Abramson T, MacKinnon R (1992) A functional connection between the pores of distantly related ion channels as revealed by mutant K channels. Science 258: 1152–1155. doi: 10.1126/science.1279807
- 38. Ardell MD, Bedsole DL, Schoborg RV, Pittler SJ (2000) Genomic organization of the human rod photoreceptor cGMP-gated cation channel β-subunit gene. Gene 245: 311–318. doi: 10.1016/S0378-1119(00)00023-8
- 39. Ardell MD, Aragon I, Oliveira L, Porche GE, Burke E, et al. (1996) The β subunit of human rod photoreceptor cGMP-gated cation channel is generated from a complex transcription unit. FEBS Lett 389: 213–218. doi: 10.1016/0014-5793(96)00588-1
- 40. Sautter A, Zong X, Hofmann F, Biel M (1998) An isoform of the rod photoreceptor cyclic nucleotide-gated channel β subunit expressed in olfactory neurons. Proceedings of the National Academy of Sciences 95: 4696–4701. doi: 10.1073/pnas.95.8.4696
- 41. Wiesner B, Weiner J, Middendorff R, Hagen V, Kaupp UB, et al. (1998) Cyclic nucleotide-gated channels on the flagellum control Ca2 entry into sperm. J Cell Biol 142: 473–484. doi: 10.1083/jcb.142.2.473
- 42. Bareil C, Hamel C, Delague V, Arnaud B, Demaille J, et al. (2001) Segregation of a mutation in CNGB1 encoding the β-subunit of the rod cGMP-gated channel in a family with autosomal recessive retinitis pigmentosa. Hum Genet 108: 328–334. doi: 10.1007/s004390100496
- 43. Kondo H, Qin M, Mizota A, Kondo M, Hayashi H, et al. (2004) A homozygosity-based search for mutations in patients with autosomal recessive retinitis pigmentosa, using microsatellite markers. Invest Ophthalmol Vis Sci 45: 4433–4439. doi: 10.1167/iovs.04-0544
- 44. Simpson DA, Clark GR, Alexander S, Silvestri G, Willoughby CE (2011) Molecular diagnosis for heterogeneous genetic diseases with targeted high-throughput DNA sequencing applied to retinitis pigmentosa. J Med Genet 48: 145–151. doi: 10.1136/jmg.2010.083568
- 45. Hüttl S, Michalakis S, Seeliger M, Luo D, Acar N, et al. (2005) Impaired channel targeting and retinal degeneration in mice lacking the cyclic nucleotide-gated channel subunit CNGB1. The Journal of neuroscience 25: 130–138. doi: 10.1523/JNEUROSCI.3764-04.2005
- 46. Koch S, Sothilingam V, Garrido MG, Tanimoto N, Becirovic E, et al. (2012) Gene therapy restores vision and delays degeneration in the CNGB1−/− mouse model of retinitis pigmentosa. Hum Mol Genet 21: 4486–4496. doi: 10.1093/hmg/dds290