MicroRNAs (miRNAs) negatively regulate the 3′ untranslated region (3′-UTR) of coding genes by suppressing translation or degrading mRNAs, and they act as oncogenes or tumor suppressors. Recently, several studies investigated the association between pre-miR-27a rs895819 polymorphism and the risks of various cancers, but the results were inconsistent.
We conducted a meta-analysis of 13 studies that included 6501 cancer cases and 7571 controls to address this association. Overall, this meta-analysis showed that the pre-miR-27a rs895819 A/G polymorphism was not statistically associated with cancers risk in all genetic models. In the stratified analysis by cancer types, when compared with the ancestral A allele, individuals with the variant G allele was consistently associated with reduced risks of breast cancer (OR = 0.92, 95% CI = 0.85–0.99), renal cell cancer (OR = 0.81, 95% CI = 0.67–0.97) and nasopharyngeal cancer (OR = 0.84, 95% CI = 0.72–0.97). Inversely, individuals with the heterozygote AG was associated with an increased risk of digestive tract cancers compared with AA genotype (AG vs. AA: OR = 1.16, 95% CI = 1.01–1.32). In the stratified analysis by ethnicity, the pre-miR-27a rs895819 polymorphism showed statistically significant association with decreased risks of cancers in Caucasians (G vs. A allele: OR = 0.90, 95% CI = 0.83–0.97; AG vs. AA: OR = 0.84, 95% CI = 0.75–0.94; AG/GG vs. AA: OR = 0.85, 95% CI = 0.76–0.94) but not in Asians.
This meta-analysis suggests that the pre-miR-27a rs895819 polymorphism may contribute to the susceptibilities of some specific-type of cancers, including breast cancer, renal cell cancer, nasopharyngeal cancer and digestive tract cancers, as well as the susceptibilities of cancers in Caucasians to some extent.
Citation: Xu Q, He C-y, Liu J-w, Yuan Y (2013) Pre-miR-27a rs895819A/G Polymorphisms in Cancer: A Meta-Analysis. PLoS ONE 8(6): e65208. doi:10.1371/journal.pone.0065208
Editor: Amanda Ewart Toland, Ohio State University Medical Center, United States of America
Received: December 25, 2012; Accepted: April 23, 2013; Published: June 7, 2013
Copyright: © 2013 Xu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work is supported by grants from the National Key Basic Research Program of China (973 Program ref no. 2010CB529304, http://www.973.gov.cn/Default_3.aspx), the National Natural Science Foundation of China (Ref No. 31200968, http://www.nsfc.gov.cn/Portal0/default152.htm), and the Science Technology Project in Liaoning Province (Ref No. 2011225002, http://kjjh.lninfo.gov.cn/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
MicroRNAs (miRNAs) are RNAs that are 18–23 nucleotides long that participate in the transcriptional regulation of eukaryotic genes , which is associated with post-transcriptional modifications that lead to the mRNA’s degradation or the translational repression of the targeted genes . miRNA genes are transcribed to a hairpin-shaped RNA form named pri-miRNA , which is processed by an endonuclease, Drosha, to form a pre-miRNA that is 60–70 nucleotides long . The pre-miRNAs are cleaved by an endonuclease, Dicer, to form the mature miRNA, which functions as a gene regulator . The variation of the pri-, pre-, and mature miRNA through physiological processes may cause the incidence and development of a tumor.
miRNAs are transcribed from a gene intron and/or intergenic region. Polymorphisms exist in the pri-, pre-, and mature miRNA genes that can potentially influence the processing of maturing miRNAs, broadly affecting the miRNA function . The first research focused on pre-miRNA polymorphisms screened 323 single nucleotide polymorphisms (SNPs) in 227 miRNA genes according to bioinformation by Duan et al. . From then on, scholars all focused on 3 SNP sites in the field of the association between miRNA polymorphism and cancers, hsa-pre-miR-146a rs2910164, hsa-pre-miR-196a2 rs11614913, and hsa-miR-499 rs374644, and several meta analyses reported that these 3 sites were associated with cancer risks –. The miR-27a rs895819 A/G polymorphism is located in the loop of the pre-miRNA, and the variation from A to G could cause a change in the minimum free energy (MFE). Therefore, this SNP may affect the function of the miRNA to some extent. However, the results regarding its association with cancer risk are conflicting. The majority of studies reported that the variant allele could decrease the cancer risk –, but some studies reported different viewpoints. A few studies reported that the association between the variant G allele and cancer risk was insignificant; these studies denied the protective role of the G allele –. Moreover, some studies reported that the variant G allele was a risk allele. For example, 2 studies using samples from the same continent analyzed the AG and GG genotypes for the risk of gastric cancer and compared the risk with that for the AA wild-type genotype. One study found that the AG and GG genotypes increased the risk of gastric cancer , and the other study found the opposite result . Therefore, the comprehensive analysis that integrates all individual studies is still required. To enhance the efficiency of meta-analysis on the risk of cancers and investigate whether this rs895819 polymorphism was associated with the gastric cancer risk, an unpublished case-control study on gastric cancer that was performed by Qian Xu et al at the Tumor Etiology and Screening Department of Cancer Institute and General Surgery in the First Affiliated Hospital of China Medical University, Shenyang, China was collected. Overall, 13 datasets consisted of 6501 cases with cancers and 7571 controls were included in our study. These data should be combined to expand our understanding of the pre-miR-27a rs895819 SNP, which may provide some evidence for the future research. Thus, in this study we conducted a meta-analysis to combine all of the available studies and validate whether the miR-27a A/G polymorphism contributes to the risks of cancers.
A systematic literature search was performed for all articles on the association between pre-miR-27a rs895819 polymorphism and cancer risks up to March 15th, 2013. The PubMed databases, Chinese National Knowledge Infrastructure (CNKI) and Web of Science were used simultaneously. The following key words were used: “miR-27a”, “cancer/carcinoma/tumor/neoplasm”, “rs895819” and “polymorphism” by two independent investigators Qian Xu and Cai-yun He. All the studies met the following criteria: (1) case-control study; (2) the association between miR-27a polymorphism and cancer risks was explored; and (3) genotype frequency was available. The major exclusion criteria were: (1) duplicate data, (2) the study only for cancer samples or for precancerous disease compared with controls, and (3) the subjects in control group were high-risk individuals with some gene mutations.
Two of the authors (Qian Xu and Cai-yun He) extracted all data independently, complied with the selection criteria, and reached a consensus on all items. The following items were collected: first author’s name, year of publication, country of origin, ethnicity, cancer type, language, genotyping method, source of control groups (population- or hospital-based), and total number of cases and controls, and genotype distributions in cases and controls. Meanwhile, we categorized gastric cancer, colorectal cancer and liver cancer into ‘digestive tract cancer’ for the stratified analysis.
Firstly, the genotype frequencies of miR-27a polymorphism for Hardy-Weinberg equilibrium (HWE) were assessed by the Chi-square test in controls and a P<0.05 was considered as significant disequilibrium. The strength of the association between the miR-27a polymorphism and cancer risks was estimated by odds ratios (ORs) with 95% confidence intervals (CIs). Statistical heterogeneity among studies was tested by Chi square-based Q test and I2 . Heterogeneity was considered significantly when P<0.10 was observed in Q test, and I2 was used to qualify variation in OR attributable to heterogeneity. When heterogeneity exists, a random-effect model based on DerSimonian and Laird method was used to calculated the pooled OR of each study ; otherwise, a fixed-effect model based on the Mantel-Haenszel method was employed . Generally, we first evaluated the risks between G allele and cancer risks compared with that for A allele in the codominant model (AG versus AA, GG versus AA), dominant model (AG/GG versus AA) and recessive model (GG versus AG/AA), and allelic comparison (G versus A). We also performed stratification analyses on cancer type (divided into digestive tract cancer, breast cancer, nasopharyngeal cancer and renal cell cancer) and ethnicity (Asian and Caucasian). Meta-regression was further performed to detect the source of heterogeneity. The between-studies variance (τ2) was used to quantify the degree of heterogeneity between studies and the percentage of τ2 was used to describe the extent of heterogeneity explained . The Begg’s rank correlation method was used to statistically assess publication bias  (P<0.10 was considered as statistically significant). All analyses were done using STATA software, version 11.0 (STATA Corp., College Station, TX, USA), and all tests were two-sided.
Characteristics of the Studies
A total of 20 articles were found by literature search from the PubMed, CNKI and Web of Science –, –, using different combinations of key terms. As shown in Figure 1, we excluded nine studies (one was function study , one was reduplicate data and showed technique problem , two were not studied for the rs895819 polymorphism , , one is case-only study , two had no qualified cases or controls , , and two were meta-analyses about the rs895819 SNP , ). In addition, our research group provided this meta-analysis with a set of unpublished data regarding the association between rs895819 polymorphism and gastric cancer risk. Accordingly, a total of 13 case-control studies extracted from 11 articles and a set of our unpublished data that met our inclusion criteria were included in the final meta-analysis –, –, which consisted of 6501 cancer cases and 7571 cancer-free controls. The characteristic of each included study was presented in Table 1. Of the enrolled 13 studies, all were matched for age, sex except 4 studies for breast cancer that did not need to match sex; 10 studies investigated in Asians and the other 3 studies investigated in Caucasians; controls in 7 studies were hospital-based and controls of the other studies were population-based or mixed subjects; genotyping methods included PCR-RFLP (3 studies), TaqMan (3 studies), PCR-based single base extension (5 studies) and PCR-direct sequencing (2 studies). 11 out of 13 studies checked genotypes for quality control. Genotype distribution of controls in all studies was consistent with Hardy-Weinberg equilibrium.
Figure 1. Studies identified in this meta-analysis based on the criteria for inclusion and exclusion.doi:10.1371/journal.pone.0065208.g001
Table 1. Characteristics of literatures included in this meta-analysis for miR-27a rs895819A/G polymorphism.doi:10.1371/journal.pone.0065208.t001
First, all the eligible studies were pooled to estimate the association strength of mir-27a rs895819 polymorphism with the overall risk of all types of cancers. No statistically significant association was found in all genetic models (Table 2). We further performed stratification analysis based on different cancer types and different ethnicities (Table 2).
Table 2. Pooled ORs and 95% CIs of the overall and stratified meta-analyses.doi:10.1371/journal.pone.0065208.t002
In the stratification analysis of cancer type, the variant G allele was observed to be statistically associated with a decreased risk of breast cancer (OR = 0.92, 95% CI = 0.85–0.99). Since only one study was conducted in renal cell cancer or nasopharyngeal cancer ,, the pooled OR could not be appraised on these two cancer types. Based on single study, we observed that the AG heterozygote and AG/GG genotypes were consistently associated with decreased risks of renal cell cancer (AG vs. AA:OR = 0.70, 95% CI = 0.55–0.89; AG/GG vs. AA: OR = 0.72, 95% CI = 0.57–0.90) and nasopharyngeal cancer (AG vs. AA:OR = 0.79, 95% CI = 0.65–0.96; AG/GG vs. AA: OR = 0.79, 95% CI = 0.66–0.95) when compared with the wild-type AA genotype. In addition, the G allele was statistically associated with decreased risks of renal cell cancer (OR = 0.81, 95% CI = 0.67–0.97) and nasopharyngeal cancer (OR = 0.84, 95% CI = 0.72–0.97) compared with the A allele. These observations insinuated a reverse association of G allele with the risks of those tumor types. Nevertheless, the comparison of AG versus AA in digestive tract cancer subgroup showed an opposite result. The AG genotype was associated with an increased risk of digestive tract cancer (AG vs. AA genotype: OR = 1.16, 95% CI = 1.01–1.32).
In the stratification analysis of ethnicity, when compared with the ancestral AA genotype, the AG heterozygote and AG/GG genotypes were associated with a decreased risk of cancers in Caucasians subgroup (AG vs. AA: OR = 0.84, 95% CI = 0.75–0.94; AG/GG vs. AA: OR = 0.85, 95% CI = 0.76–0.94) but not in Asians subgroup. We observed the same association in the allelic comparison (G allele vs. A allele: OR = 0.90, 95% CI = 0.83–0.97).
The heterogeneity within each subgroup was shown in Table 2. A part of comparisons showed slight or moderate heterogeneities between studies. We subsequently conducted sensitivity analyses to explore individual study’s influence on the pooled results by the removing one study at a time from pooled analysis (Table S1). The results showed no individual study affected the pooled OR significantly. We therefore further explored the source of heterogeneity by cancer type, ethnicity, language, and genotyping method with meta-regression in allelic comparison (G vs A). Meta-regression results revealed that cancer type (P = 0.023) but not ethnicity (P = 0.452), language (P = 0.347), or genotyping method (P = 0.100) contributed to the source of heterogeneity. Additionally, cancer type could explained 85.29% of the between studies variance (τ2).
Begg’s rank correlation was conducted to evaluate publication bias. In the comparison of AG vs. AA and dominant model (AG/GG versus AA), a slight publication bias was observed (AG vs. AA: P = 0.020; AG/GG versus AA: P = 0.088, respectively, Table 3), insinuating potential publication bias due to a language bias, inflated estimates by a flawed methodological design in smaller studies, and/or a lack of publication of small trials with opposite results.
miR-27a, which is located in an intergenic region of chromosome 19 (chr19:13947254–13947331), had up-regulated expression in many tumors  and was considered to be an oncomir –. Using the single-stranded conformational polymorphism-polymerase chain reaction (PCR-SSCP) method Tomiyasu Arisawa discovered in 2007 that there was a polymorphism in the flanking region of miR-27a gene, and the variant G allele frequency reached 34.6% . In the same year, Ranhui Duan et al. employed a bioinformatic method to screen 323 SNPs, and emphasized the importance of this rs895819 SNP because of its location in the pre-miRNA sequence . Since then, several studies of this SNP emerged in succession. In 2010, Tair Kontorovich et al., using a Sequenom MassARRAY, screened 42 miRNA-related SNPs and found that AG heterozygote of miR-27a rs895819 had a lower risk for breast/ovarian cancer among Jewish women . At almost the same time, Rongxi Yang et al. used a sequencing method to observe that the variant G allele significantly decreased familial breast cancer risk in a German population . Following that, it was suggested that the AG and GG genotypes had a decreased renal cell cancer risk in a Chinese population compared with the AA wild-type genotype . However, some studies hold different viewpoints; a few studies considered the relationship between the variant G allele and the risk of tumor to be insignificant –. Moreover, some researchers thought that the variant G allele was a risk allele. Two inconsistent studies investigated the risk of gastric cancer using samples from the same country. Qingmin Sun et al. found that the AG and GG genotypes were associated with an increased risk of gastric cancer and an increased expression level of miR-27a , but Yuan Zhou observed the opposite result .
According to our meta-analysis, A→G variation at miR-27a rs895819 polymorphism site did not exert significant genetic effect on cancer risk in the overall analysis (Table 2). We further performed stratification analysis based on cancer type and ethnicity. In breast cancer subgroup analysis integrating 4 studies, subjects carrying G allele of rs895819 demonstrated reduced cancer risk. Additionally, one study about renal cell cancer  and one study on nasopharyngeal cancer  could not be incorporated into other cancer types and thus were analyzed separately as single cancer type in this meta-analysis. For renal cell cancer and nasopharyngeal cancer, association analyses under codominant model (AG versus AA), dominant model (AG/GG versus AA) and allelic comparison (G vs. A) consistently supported that this polymorphism was related to decreased risks of both cancers. The slight difference of ORs and 95% CI between our meta-analysis and their original studies was probably due to unavailable raw data for adjustment of other factors. All these findings indicated that this polymorphism may be associated with decreased risks of specific cancers including breast cancer, renal cell carcinoma and nasopharyngeal carcinoma.
In subgroup analysis of digestive tract cancer, however, we observed that AG genotype was associated with increased cancer risk. As for the single original study, Sun et al ’s  reported a statistically positive association of rs895819 polymorphism with gastric cancer risk; four studies , ,  (Qian Xu, 2013*) showed that this polymorphism tended to be positively associated with higher risks of digestive tract cancers, although they did not reach statistical significance. In this meta-analysis, we incorporated and evaluated the available data of this polymorphism with digestive tract cancer risk, which may demonstrate a relatively stable result. The difference of the association of this polymorphism with various cancers may result from distinct cell origin and pathogenetic mechanisms of different cancers.
To better elucidate the association of this polymorphism with digestive tract cancer, we added a set of unpublished data which was a case-control study including 222 gastric cancer cases and 305 superficial gastritis patients as controls in Chinese. The control group contained participants from a health check program for gastric cancer screening in Zhuanghe region of Liaoning Province, China and patients who underwent gastroscopic examination in the First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China. All the 527 subjects were diagnosed independently by two professorial pathologists. By integrating currently-published 6 studies and our newly-unpublished data, we found for the first time that this polymorphism may be associated with digestive tract cancer, which is also one of the differences between our meta-analysis and previously published meta-analyses. Furthermore, considering the relatively obvious heterogeneity observed in this meta-analysis even after employing sensitivity analyses and subgroup analyses, we carried out meta-regression to further explore the source of heterogeneity. Interestingly, the results of meta-regression indicated the heterogeneity mainly resulted from different cancer types and this variance could explain the majority of heterogeneity origin. Therefore, the different effects of this polymorphism on various cancers should not be overlooked and its specific effects on different cancer types are warranted to be clarified in future research.
Limitations of this study should also be noted. First, we pooled the data using unadjusted information, whereas a more precise analysis could to be conducted if detailed information of original data is available. Second, a lack of original data of the reviewed studies limited our further evaluation of potential interactions, including the interactions between different genes and between gene and environment factors. Third, only English and Chinese documents and our unpublished study were included in this meta-analysis, while reports that were written in other languages and other unpublished data or ongoing studies were not available, which may cause certain publication bias in our meta-analysis. The last but not the least, the pooled sample size was relatively limited in this meta-analysis. Therefore, this meta-analysis could only preliminarily appraise the association of rs895819 polymorphism with currently-reported cancers. More studies are still required to get a more reliable result.
In summary, this meta-analysis indicated that the G allele of miR-27a rs895819 polymorphism may be associated with decreased risks of breast cancer, renal cell cancer and nasopharyngeal cancer, as well as a decreased cancer risk in Caucasians and AG heterozygote might be associated with an increased risk of digestive tract cancer. To confirm our findings, additional well-designed studies in diverse ethnic populations and functional studies regarding this SNP are required.
ORs (95% CI) of sensitivity analysis.
Checklist of this meta-analysis.
Conceived and designed the experiments: YY. Performed the experiments: QX. Analyzed the data: QX CH. Contributed reagents/materials/analysis tools: QX CH. Wrote the paper: QX. Revised the manuscript: QX CH JL YY.
- 1. Zanetti KA, Haznadar M, Welsh JA, Robles AI, Ryan BM, et al. (2012) 3′-UTR and functional secretor haplotypes in mannose-binding lectin 2 are associated with increased colon cancer risk in African Americans. Cancer Res 72: 1467–1477. doi: 10.1158/0008-5472.can-11-3073
- 2. Khvorova A, Reynolds A, Jayasena SD (2003) Functional siRNAs and miRNAs exhibit strand bias. Cell 115: 209–216. doi: 10.1016/s0092-8674(03)00801-8
- 3. Higgins JP, Thompson SG, Deeks JJ, Altman DG (2003) Measuring inconsistency in meta-analyses. Bmj 327: 557–560. doi: 10.1136/bmj.327.7414.557
- 4. Lee Y, Ahn C, Han J, Choi H, Kim J, et al. (2003) The nuclear RNase III Drosha initiates microRNA processing. Nature 425: 415–419. doi: 10.1038/nature01957
- 5. Schwarz DS, Hutvagner G, Du T, Xu Z, Aronin N, et al. (2003) Asymmetry in the assembly of the RNAi enzyme complex. Cell 115: 199–208. doi: 10.1016/s0092-8674(03)00759-1
- 6. Duan R, Pak C, Jin P (2007) Single nucleotide polymorphism associated with mature miR-125a alters the processing of pri-miRNA. Hum Mol Genet 16: 1124–1131. doi: 10.1093/hmg/ddm062
- 7. Wang L, Qian S, Zhi H, Zhang Y, Wang B, et al. (2012) The association between hsa-miR-499 T>C polymorphism and cancer risk: a meta-analysis. Gene 508: 9–14. doi: 10.1016/j.gene.2012.08.005
- 8. Lian H, Wang L, Zhang J (2012) Increased risk of breast cancer associated with CC genotype of Has-miR-146a Rs2910164 polymorphism in Europeans. PLoS One 7: e31615. doi: 10.1371/journal.pone.0031615
- 9. Guo J, Jin M, Zhang M, Chen K (2012) A genetic variant in miR-196a2 increased digestive system cancer risks: a meta-analysis of 15 case-control studies. PLoS One 7: e30585. doi: 10.1371/journal.pone.0030585
- 10. Qiu LX, He J, Wang MY, Zhang RX, Shi TY, et al. (2012) The association between common genetic variant of microRNA-146a and cancer susceptibility. Cytokine 56: 695–698. doi: 10.1016/j.cyto.2011.09.001
- 11. Wang J, Bi J, Liu X, Li K, Di J, et al. (2012) Has-miR-146a polymorphism (rs2910164) and cancer risk: a meta-analysis of 19 case-control studies. Mol Biol Rep 39: 4571–4579. doi: 10.1007/s11033-011-1247-7
- 12. Qiu LX, Wang Y, Xia ZG, Xi B, Mao C, et al. (2011) miR-196a2 C allele is a low-penetrant risk factor for cancer development. Cytokine 56: 589–592. doi: 10.1016/j.cyto.2011.08.019
- 13. Xu W, Xu J, Liu S, Chen B, Wang X, et al. (2011) Effects of common polymorphisms rs11614913 in miR-196a2 and rs2910164 in miR-146a on cancer susceptibility: a meta-analysis. PLoS One 6: e20471. doi: 10.1371/journal.pone.0020471
- 14. Wang F, Ma YL, Zhang P, Yang JJ, Chen HQ, et al. (2012) A genetic variant in microRNA-196a2 is associated with increased cancer risk: a meta-analysis. Mol Biol Rep 39: 269–275. doi: 10.1007/s11033-011-0735-0
- 15. Chu H, Wang M, Shi D, Ma L, Zhang Z, et al. (2011) Hsa-miR-196a2 Rs11614913 polymorphism contributes to cancer susceptibility: evidence from 15 case-control studies. PLoS One 6: e18108. doi: 10.1371/journal.pone.0018108
- 16. Gao LB, Bai P, Pan XM, Jia J, Li LJ, et al. (2011) The association between two polymorphisms in pre-miRNAs and breast cancer risk: a meta-analysis. Breast Cancer Res Treat 125: 571–574. doi: 10.1007/s10549-010-0993-x
- 17. Kontorovich T, Levy A, Korostishevsky M, Nir U, Friedman E (2010) Single nucleotide polymorphisms in miRNA binding sites and miRNA genes as breast/ovarian cancer risk modifiers in Jewish high-risk women. Int J Cancer 127: 589–597. doi: 10.1002/ijc.25065
- 18. Yang R, Schlehe B, Hemminki K, Sutter C, Bugert P, et al. (2010) A genetic variant in the pre-miR-27a oncogene is associated with a reduced familial breast cancer risk. Breast Cancer Res Treat 121: 693–702. doi: 10.1007/s10549-009-0633-5
- 19. Shi D, Li P, Ma L, Zhong D, Chu H, et al. (2012) A Genetic Variant in pre-miR-27a Is Associated with a Reduced Renal Cell Cancer Risk in a Chinese Population. PLoS One 7: e46566. doi: 10.1371/journal.pone.0046566
- 20. Zhang M, Jin M, Yu Y, Zhang S, Wu Y, et al. (2012) Associations of miRNA polymorphisms and female physiological characteristics with breast cancer risk in Chinese population. Eur J Cancer Care (Engl) 21: 274–280. doi: 10.1111/j.1365-2354.2011.01308.x
- 21. Hezova R, Kovarikova A, Bienertova-Vasku J, Sachlova M, Redova M, et al. (2012) Evaluation of SNPs in miR-196-a2, miR-27a and miR-146a as risk factors of colorectal cancer. World J Gastroenterol 18: 2827–2831. doi: 10.3748/wjg.v18.i22.2827
- 22. Catucci I, Verderio P, Pizzamiglio S, Bernard L, Dall'olio V, et al. (2012) The SNP rs895819 in miR-27a is not associated with familial breast cancer risk in Italians. Breast Cancer Res Treat 133: 805–807. doi: 10.1007/s10549-012-2011-y
- 23. Sun Q, Gu H, Zeng Y, Xia Y, Wang Y, et al. (2010) Hsa-mir-27a genetic variant contributes to gastric cancer susceptibility through affecting miR-27a and target gene expression. Cancer Sci 101: 2241–2247. doi: 10.1111/j.1349-7006.2010.01667.x
- 24. Zhou Y, Du WD, Chen G, Ruan J, Xu S, et al. (2012) Association analysis of genetic variants in microRNA networks and gastric cancer risk in a Chinese Han population. J Cancer Res Clin Oncol 138: 939–945. doi: 10.1007/s00432-012-1164-8
- 25. Lau J, Ioannidis JP, Schmid CH (1997) Quantitative synthesis in systematic reviews. Ann Intern Med 127: 820–826. doi: 10.7326/0003-4819-127-9-199711010-00008
- 26. DerSimonian R, Laird N (1986) Meta-analysis in clinical trials. Control Clin Trials 7: 177–188. doi: 10.1016/0197-2456(86)90046-2
- 27. Mantel N, Haenszel W (1959) Statistical aspects of the analysis of data from retrospective studies of disease. J Natl Cancer Inst 22: 719–748.
- 28. Whitehead A, Whitehead J (1991) A general parametric approach to the meta-analysis of randomized clinical trials. Stat Med 10: 1665–1677. doi: 10.1002/sim.4780101105
- 29. Begg CB, Mazumdar M (1994) Operating characteristics of a rank correlation test for publication bias. Biometrics 50: 1088–1101. doi: 10.2307/2533446
- 30. Zhong S, Chen Z, Xu J, Li W, Zhao J (2013) Pre-mir-27a rs895819 polymorphism and cancer risk: a meta-analysis. Mol Biol Rep 40: 3181–3186. doi: 10.1007/s11033-012-2392-3
- 31. Yang Q, Jie Z, Ye S, Li Z, Han Z, et al.. (2012) Genetic variations in miR-27a gene decrease mature miR-27a level and reduce gastric cancer susceptibility. Oncogene [Epub ahead of print].
- 32. Yoon KA, Yoon H, Park S, Jang HJ, Zo JI, et al. (2012) The prognostic impact of microRNA sequence polymorphisms on the recurrence of patients with completely resected non-small cell lung cancer. J Thorac Cardiovasc Surg 144: 794–807. doi: 10.1016/j.jtcvs.2012.06.030
- 33. Yang R, Burwinkel B (2012) A bias in genotyping the miR-27a rs895819 and rs11671784 variants. Breast Cancer Res Treat 134: 899–901. doi: 10.1007/s10549-012-2140-3
- 34. Hirota T, Date Y, Nishibatake Y, Takane H, Fukuoka Y, et al. (2012) Dihydropyrimidine dehydrogenase (DPD) expression is negatively regulated by certain microRNAs in human lung tissues. Lung Cancer 77: 16–23. doi: 10.1016/j.lungcan.2011.12.018
- 35. Jahid S, Sun J, Edwards RA, Dizon D, Panarelli NC, et al. (2012) miR-23a promotes the transition from indolent to invasive colorectal cancer. Cancer Discov 2: 540–553. doi: 10.1158/2159-8290.cd-11-0267
- 36. Arisawa T, Tahara T, Shibata T, Nagasaka M, Nakamura M, et al. (2007) A polymorphism of microRNA 27a genome region is associated with the development of gastric mucosal atrophy in Japanese male subjects. Dig Dis Sci 52: 1691–1697. doi: 10.1007/s10620-006-9648-5
- 37. Zhang M-w (2012) Study on the Associations of Lifestyle-related Factors,Gentic Variants in miRNA Encoding Regions and miRNA Binding Sites with Colorectal Cancer Risk. Zhejiang University: [D] Zhejiang.
- 38. Li P-r (2011) Genetic Associations of miRNA-SNPs with the Common Diseases and Integrative Analysis of "OMICS" Data of Hepatocellular Carcinoma. Academy of Military Medical Sciences in the People’s Liberarion Army: [D] Beijing.
- 39. Zhang P (2011) Polymorphisms of MicroRNA and ESR1 Genes and Their Association with Triple Negative Breast Cancer Risk and Prognosis. Chinese Academy of Medical Sciences (Beijing Union Medical College): [D] Beijing.
- 40. Wang W-z (2011) The Association of miR-146a Single Nucleotide Polymorphisms and Genetic Susceptibility of Liver Cancer and Study of Tumor-associaed microRNA Microarray analysis. Fudan University: [D] Shanghai.
- 41. Wang B, Ma N, Wang Y (2012) Association Between the hsa-mir-27a Variant and Breast Cancer Risk: a Meta-analysis. Asian Pac J Cancer Prev 13: 6207–6210. doi: 10.7314/apjcp.2012.13.12.6207
- 42. Ma Y, Yu S, Zhao W, Lu Z, Chen J (2010) miR-27a regulates the growth, colony formation and migration of pancreatic cancer cells by targeting Sprouty2. Cancer Lett 298: 150–158. doi: 10.1016/j.canlet.2010.06.012
- 43. Liu T, Tang H, Lang Y, Liu M, Li X (2009) MicroRNA-27a functions as an oncogene in gastric adenocarcinoma by targeting prohibitin. Cancer Lett 273: 233–242. doi: 10.1016/j.canlet.2008.08.003
- 44. Zhang Z, Liu S, Shi R, Zhao G (2009) miR-27 promotes human gastric cancer cell metastasis by inducing epithelial-to-mesenchymal transition. Cancer Genet 204: 486–491. doi: 10.1016/j.cancergen.2011.07.004
- 45. Wang Q, Li DC, Li ZF, Liu CX, Xiao YM, et al. (2011) Upregulation of miR-27a contributes to the malignant transformation of human bronchial epithelial cells induced by SV40 small T antigen. Oncogene 30: 3875–3886. doi: 10.1038/onc.2011.103