Figures
Abstract
Worldwide, ticks are important vectors of human and animal pathogens. Besides Lyme Borreliosis, a variety of other bacterial and protozoal tick-borne infections are of medical interest in Europe. In this study, 553 questing and feeding Ixodes ricinus (n = 327) and Dermacentor reticulatus ticks (n = 226) were analysed by PCR for Borrelia, Rickettsia, Anaplasma, Coxiella, Francisella and Babesia species. Overall, the pathogen prevalence in ticks was 30.6% for I. ricinus and 45.6% for D. reticulatus. The majority of infections were caused by members of the spotted-fever group rickettsiae (24.4%), 9.4% of ticks were positive for Borrelia burgdorferi sensu lato, with Borrelia afzelii being the most frequently detected species (40.4%). Pathogens with low prevalence rates in ticks were Anaplasma phagocytophilum (2.2%), Coxiella burnetii (0.9%), Francisella tularensis subspecies (0.7%), Bartonella henselae (0.7%), Babesia microti (0.5%) and Babesia venatorum (0.4%). On a regional level, hotspots of pathogens were identified for A. phagocytophilum (12.5–17.2%), F. tularensis ssp. (5.5%) and C. burnetii (9.1%), suggesting established zoonotic cycles of these pathogens at least at these sites. Our survey revealed a high burden of tick-borne pathogens in questing and feeding I. ricinus and D. reticulatus ticks collected in different regions in Belarus, indicating a potential risk for humans and animals. Identified hotspots of infected ticks should be included in future surveillance studies, especially when F. tularensis ssp. and C. burnetii are involved.
Citation: Reye AL, Stegniy V, Mishaeva NP, Velhin S, Hübschen JM, Ignatyev G, et al. (2013) Prevalence of Tick-Borne Pathogens in Ixodes ricinus and Dermacentor reticulatus Ticks from Different Geographical Locations in Belarus. PLoS ONE 8(1): e54476. https://doi.org/10.1371/journal.pone.0054476
Editor: Roman Ganta, Kansas State University, United States of Ameica
Received: March 12, 2012; Accepted: December 12, 2012; Published: January 22, 2013
Copyright: © 2013 Reye et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This study was funded by the Ministry of Foreign Affairs of the Grand-Duchy of Luxembourg, the Centre de Recherche Public de la Santé and the Republic Fond of Fundamental Research of the Academy of Science of Belarus. A.L. Reye was supported by a fellowship from the Bourse Formation Recherche and the Aides à la Formation Recherche of the Fonds National de la Recherche Luxembourg (BFR 07/038; EXT-BFR07-038 TR). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Introduction
Throughout the world, ticks are important vectors of human and animal pathogens. In Eastern Europe, Lyme Borreliosis (LB) is a major public health threat with annual incidence rates ranging from 4.8 (Poland) to 35 (Lithuania) cases per 100.000 population [1]. In Belarus, 1.0 to 9.1 cases per 100.000 have been reported over a ten year period [2]. Although early manifestations of the disease usually can be diagnosed and treated successfully, late manifestations such as Lyme arthritis, Acrodermatitis chronica atrophicans and neuroborreliosis can be severe. The causative agents are members of the Borrelia burgdorferi sensu lato (s.l.) complex, of which at least Borrelia burgdorferi sensu stricto (s.s.), B. afzelii and B. garinii are known to be human pathogens, whereas pathogenicity has not been confirmed for B. spielmanii, B. lusitaniae and B. valaisiana. In the neighbouring countries of Belarus, the prevalence of Borrelia species in questing Ixodes ticks ranges from 6.2% in Poland, 11% in Lithuania to 40.7% in Russia and 18–51% in Latvia [3]–[6]. No such prevalence data are available for Belarus, but the three human pathogenic Borrelia species have been isolated from ticks before [7].
Six other tick-borne bacterial and protozoal pathogen genera are of medical interest in Europe. Rickettsia species of the spotted fever group (SFG) can cause rickettsioses in humans, which are mainly characterized by feverish infections. Little is known about the incidence of spotted fever rickettsioses in Eastern Europe, but surveillance studies revealed that Rickettsia prevalence in ticks ranges from about 3% in Poland and 6% in the Ukraine and Slovakia to 15% in Russia [8]–[11].
Anaplasma phagocytophilum is another rickettsial agent pathogenic for humans, causing human granulocytic anaplasmosis. Although the incidence of anaplasmosis in Europe is not well documented, clinical cases seem to be rare. In ticks, a prevalence of 2.9% in Lithuania, 2.9–8.7% in Poland, 3.6% in the Ukraine and 5.0% in Russia have been observed [3], [4], [10], [12], [13].
Coxiella burnetii is the causative agent of Q fever in humans, but it also affects domestic and wild ruminants, leading to increased rates of abortion. Although ticks are competent vectors of this pathogen, so far Q fever outbreaks have been linked to exposure to infected animals and their products. Despite only limited studies the prevalence of C. burnetii in European ticks, does not seem to exceed 2.6% [9], [14]–[16].
Another tick-borne pathogen that can also be transmitted by aerosol is Francisella tularensis, causing tularaemia in humans. Prevalence rates in ticks are at least in France and Germany below 1.6% [17], [18] and like Q fever, tularaemia outbreaks are usually linked to the exposure to contaminated biological matter (faeces, blood, milk, etc.) rather than tick bites.
Bartonella species can be transmitted to humans by various arthropods including ticks and cause diseases like trench fever and cat scratch disease. The prevalence of these bacteria in ticks can vary as much as from 3.7% in Poland to 40% in Russia and France [19]–[21].
Besides these bacterial agents, also human pathogenic protozoans can be transmitted by tick bites. At least two Babesia species, namely B. microti and B. divergens, are known to cause babesiosis in humans, whereas for B. venatorum (previously Babesia sp. EU1) human pathogenicity is suspected. In Poland, 3.5% to 5.4% of ticks were found to be infected with B. microti [22], [23], whereas 1.6% of ticks from Russia were infected with B. venatorum [8].
The wide distribution of bacterial and protozoal agents of human pathogenicity and the extreme range of their prevalence in ticks indicate the need for comprehensive studies on tick-borne pathogens in particular in Eastern Europe. So far, comparatively few studies on the prevalence of tick-borne pathogens in ticks from Belarus have been published in peer-reviewed journals [24], [25]. However, official data on LB incidence and limited information on Borrelia burgdorferi s.l. prevalence in ticks exist [2]. In this study, we investigated the prevalence of seven tick-borne pathogen genera of medical interest in questing and feeding Ixodes ricinus and Dermacentor reticulatus ticks in Belarus.
Materials and Methods
Belarus is a landlocked country in North-Eastern Europe with a population of 9.4 million, 70% of which reside in urban areas. Its terrain is generally flat, with an average elevation of 160m above sea level and about 40% of the country is covered by forest. The most common tick species in Belarus are Ixodes ricinus and Dermacentor reticulatus [2].
In April and May 2009, ticks were collected from the vegetation and from cows at 32 collection sites in the administrative regions of Brest (n = 8), Gomel (n = 7), Grodno (n = 2), Minsk (n = 3), Mogilev (n = 7) and Vitebsk (n = 5) (Figure 1). One tick was removed from a dog in Minsk region. Verbal authorisation for collection of feeding ticks was obtained from farmers and animal owners. Collection of questing ticks was performed with the cloth-dragging method; feeding ticks were removed with forceps. Ticks were identified to species level using standard morphological identification keys [26] and stored in 70% ethanol at 4°C. Ticks were washed three times in 1x phosphate buffered saline, rinsed with distilled water and dried on sterile filter paper prior to DNA extraction. Disruption and homogenization was performed in lysis buffer of the InviMag Tissue DNA Mini kit (Invitek, Berlin, Germany) using the TissueLyser II (Qiagen, Venlo, Netherlands) and 5 mm stainless steel beads. The KingFisher 96 Magnetic Particle Processor (Thermo Scientific, Waltham, Massachusetts, USA) was used for the extraction of DNA according to the manufacturers’ instructions. Detection PCRs for A. phagocytophilum, Bartonella sp., Babesia sp., Borrelia sp., C. burnetii, Francisella sp. and Rickettsia sp. were carried out using 5µl of raw DNA extract and 1µl of first round products. In addition to the 17kDa PCR used for detection of Rickettsia species, a second PCR assay based on the ompA gene was performed for better species discrimination. Primer sequences and PCR conditions are specified in Table 1. In each PCR run, cloned fragments of target genes of the respective pathogens were included as specific positive controls while distilled water was used as negative control. Spike experiments with culture extract from Borrelia garinii showed a similar PCR sensitivity in DNA extracts derived from feeding and questing ticks.
Sequencing and phylogenetic analyses were carried out as described before [14] and sequences are available at NCBI under accession numbers JQ711209-JQ711262 and JX648098-JX648109. Statistical analyses were performed with Fisher’s exact test (whenever a cell value of the contingency table was equal to or below 5) or Pearson’s goodness of fit chi-square (GFX) test; P values < 0.05 are given in the text.
Results
Tick collection
In total, 553 ticks belonging either to the species Ixodes ricinus (59.1%; 327/553) or Dermacentor reticulatus (40.9%; 226/553), were collected from the vegetation (81.9%; 453/553), cattle (17.9%; 99/553) and a dog (0.2%; 1/553). The majority of ticks collected from the vegetation were I. ricinus (63.8%; 289/453), whereas from cattle mostly D. reticulatus was removed (61.6%; 61/99) (Table 2). Most ticks were collected in Gomel region (53.7%; 297/553), followed by Brest (14.8%; 82/553) and Minsk region (14.3%; 79/553). Only few ticks were collected in Grodno (8.1%; 45/553), Mogilev (5.6%; 31/553) and Vitebsk region (3.4%; 19/553) (Table 2). Adults were the predominant instars (99.6%; 551/553) comprising of 59.5% females (329/553) and 40.1% males (222/553). Only two nymphal I. ricinus (0.4%) were collected from the vegetation (Table 2). In the regions Brest, Gomel and Minsk, both tick species were equally prevalent (50 ±5%), whereas I. ricinus was predominant in Mogilev, Grodno and Vitebsk (67.7–97.8%) (Table 2). The overall prevalence of infected questing ticks from Belarus (counting mixed infected ticks only once) was 37.7% (171/453). Questing I. ricinus displayed an infection prevalence of 32.9% (95/289), which was significantly lower than for D. reticulatus (46.3%; 76/164; p<0.01) (Table 3). The overall prevalence of infections in feeding ticks was 32% (32/100); significantly fewer feeding I. ricinus ticks were positive in the pathogen detection PCRs (13.2%; 5/38) than feeding D. reticulatus ticks (43.5%; 27/62; p<0.001). On a regional level, there were considerable differences in the total prevalence of infected ticks, ranging from 10.5% in Vitebsk to 46.3% in Brest (see Figure 1).
Rickettsiaceae
Spotted Fever Group (SFG) Rickettsia were detected in 24.4% (135/553) of ticks using the 17kDa PCR assay, of which 22.2% (30/135) were identified as Rickettsia helvetica and 3.7% (5/135) clustered with Rickettsia monacensis/Rickettsia tamurae (Figure 2A; Table 3). The remaining sequences (74.1%; 100/135) clustered with the Rickettsia species R. amblyommii, R. conorii, R. heilongjiangii, R. honei, R. japonica, R. marmionii, R. montana, R. montanensis, R. parkeri, R. peacockii, R. rhipicephali, R. rickettsia, R. sibirica, and R. slovaca, hereafter referred to as the Rickettsia rickettsii group (RRG) (Figure 2A; Table 3). From the latter 105 samples, the discriminatory ompA PCR generated 65 amplicons (61.9%; 65/105), corresponding to R. monacensis (5/5) and Rickettsia raoultii (60/100) (Table 3). Forty RRG positive samples were negative in the ompA PCR assay.
Neighbour-Joining trees based on (A) a 344 nt fragment of the 17-kDa gene of Rickettsia species (nt 1194706 –1195039 of CP000766.2), (B) a 395 nt fragment of the ompA gene of Rickettsia species (nt 71 –465 of JN400406.1), (C) a 348 nt fragment of the FlaB gene of Borrelia burgdorferi s.l. (nt 97–444 of HM345909.1), (D) a 352 nt fragment of the groEL gene of Anaplasma species (nt 732–1083 of HQ629903.1), (E) a 319 nt fragment of the htpB gene of Coxiella burnetii (nt 320–638 of EU888863.1), (F) a 894 nt fragment of the 16S rRNA gene of Francisella species (nt 8–898 of HM371361.1), (G) a 515 nt fragment of the 18S rRNA gene of Babesia species (nt 2–516 of GQ856653.1) and (H) a 320 nt fragment of the groEL gene of Bartonella species (nt 14–333 of GU827129.1). Sequences from Belarus are indicated by solid circles (Ixodes ricinus) or triangles (Dermacentor reticulatus) and named with their unique identifier, tick species, geographic location, biological source and WHO country code. Only sequences of the denoted lengths were included in the displayed phylogenies. The number of sequences from Belarus within a compressed cluster and the tick species are given in brackets. BLRS, sequences from Belarus obtained in this study; I.r. = Ixodes ricinus; D.r. = Dermacentor reticulatus; RRG, Rickettsia rickettsii group; Veg = Vegetation, BLR, Belarus. Only bootstrap values above 60 are shown.
The Rickettsia infection rate was significantly higher (p<0.01) in D. reticulatus (43.8%; 99/226) than I. ricinus (11.0%; 36/327). In questing D. reticulatus ticks, RRG was the most prevalent species (22.0%; 36/164), followed by R. raoultii (19.5%; 39/99) (Table 3). Feeding D. reticulatus ticks were predominantly infected by R. raoultii (32.3%; 20/62), followed by RRG (4.8%; 3/62). The only D. reticulatus tick harbouring R. helvetica (1.0%; 1/99) was feeding on a dog. In questing I. ricinus ticks, the prevalence was 10.0% (29/289) for R. helvetica and 1.7% (5/289) for R. monacensis; R. raoultii and RRG were only detected in feeding I. ricinus (5.3%; 2/38) (Table 3). On a regional level, R. raoultii displayed highest prevalence rates in ticks collected in the regions of Brest (20.7%; 17/82) and Mogilev (16.1%; 5/31), whereas ticks positive for RRG and R. helvetica were predominantly found in Gomel (9.8%; 29/297) and Grodno region (15.6%; 7/45), respectively. All R. monacensis infected ticks were collected at the same site in Minsk region.
Borrelia species
Borrelia burgdorferi sensu lato was the second most prevalent pathogen and detected in 9.4% (52/553) of all ticks. The predominant species was B. afzelii (40.4%; 21/52) followed by B. garinii (21.2%; 11/52), B. burgdorferi sensu stricto (s.s.) (19.2%; 10/52), B.valaisiana (17.3%; 9/52) and B. lusitaniae (1.9%; 1/52) (Table 3, Figure 2B). The Borrelia prevalence was significantly higher (p<0.05) in I. ricinus (14.1%; 46/327) than in D. reticulatus (2.7%; 6/226). I. ricinus ticks were infected with all Borrelia species detected, whereas in D. reticulatus ticks only B. burgdorferi s.s., B. afzelii and B. valaisiana were detected (Table 3, Figure 2B). In ticks from the vegetation, the Borrelia prevalence (10.4%; 47/453) was twice as high as in those from host animals (5.0%; 5/100) (Table 3). Interestingly, the prevalence of Borrelia infected ticks was considerably higher in Brest (15.9%; 13/82) and Grodno (15.6%; 7/45) than in Gomel (7.7%; 23/297; p<0.05) and the remaining regions (5.3%–9.7%; not significant). Borrelia species diversity was highest in ticks from Gomel (all 5 species detected) and lowest in Vitebsk region (only B. garinii).
Low prevalent pathogens
The other pathogens were exclusively detected in questing ticks. Anaplasma phagocytophilum (2.6%; 12/453), Coxiella burnetii (1.1%; 5/453), Francisella tularensis ssp. (0.9%; 4/453), Babesia microti (0.7%; 3/453) and Babesia venatorum (0.4%; 2/453) were only detected in I. ricinus ticks, whereas both tick species harboured Bartonella henselae (0.9%; 4/453) (Table 3, Figures 2C-G). A. phagocytophilum was detected in three regions and its prevalence in ticks was considerably higher in Minsk (6.3%; 8/79) than in Gomel (2.0%; 6/297; p<0.05) and Grodno (2.2%; 1/45; not significant). Ticks from Brest and Gomel region were infected with B. henselae and both Babesia species, whereas only ticks from Gomel harboured F. tularensis ssp. and C. burnetii.
Pathogens in questing and feeding ticks
Overall, the pathogen species composition was significantly more diverse in questing than in feeding ticks (14 vs. 4 species; p<0.05). Questing I. ricinus ticks were infected with more pathogen species than questing D. reticulatus (13 vs. 5 species; not significant) (Table 4). Interestingly, the pathogen prevalence in I. ricinus was significantly lower in feeding than in questing ticks (13.2% vs. 32.9%; p<0.01), whereas it was similar in D. reticulatus ticks (42.6% vs. 46.3%; not significant). Mixed infections were detected in 2.5% (14/553) of ticks, the majority of which were formed between members of the two most prevalent pathogen genera Rickettsia and Borrelia (57.1%; 8/14) (Table 5). Also, mixed infections occurred more often in I. ricinus than in D. reticulatus ticks (3.4% vs. 1.3%; not significant). Gender specific differences in the pathogen prevalence in ticks could not be evaluated due to the low number of feeding male ticks (n = 5).
Discussion
This is the first comprehensive study on tick-borne bacterial and protozoan pathogens of human and veterinary interest in Eastern Europe. In this study, the ticks collected both from the vegetation and from hosts were mostly adults. This finding is not surprising for ticks removed from cows, as larger mammals serve as the main blood meal host for adult ticks, whereas immature ticks preferably feed on smaller vertebrates. In contrast, on vegetation immature tick stages are normally more abundant than adults due to high interstadial mortality rates [27]. Although cloth dragging has been described as a suitable collection method also for nymphs [28], our results nevertheless suggest that our collection was biased towards adult ticks.
In questing I. ricinus ticks, we observed a prevalence of Rickettsia species of 11.7%, which was similar to that reported from questing adult I. ricinus ticks from Poland (7.8–11.1%) and Slovakia (6.1%) [9], [11], [29]. Also, the prevalence of Rickettsia in questing D. reticulatus (44.5%) was comparable to reports from Poland (40.7%) [30]. Although not all 100 RRG samples were positive in the discriminatory ompA PCR, all amplified sequences allowed the identification of R. raoultii in at least one tick from each RRG positive collection site. R. raoultii and RRG were almost exclusively detected in D. reticulatus, only two feeding I. ricinus ticks were found to be infected by these rickettsiae. This suggests a high adaptation of these bacteria to the former tick species and indeed, high rates of transovarial transmission of 90% to 100% have been reported for R. raoultii in D. reticulatus and D. marginatus ticks [31].
The observed prevalence of R. raoultii of 22.6% in questing D. reticulatus was comparable to the 22.3% reported from Slovakia [32]. Although R. raoultii is endemic in Poland and Russia [31], [33], the prevalence in ticks is not known. R. raoultii is a member of the spotted fever group rickettsiae and is suspected to cause tick-borne lymphadenopathy (TIBOLA) in humans [34]. Despite the high prevalence of infected D. reticulatus and their implication in human TIBOLA infections, there are no such reports from Belarus available.
The overall prevalence of Borrelia infected I. ricinus collected from the vegetation was 12.5%, which is in the lower range of the prevalences of 10.1–32.7% reported for questing adult I. ricinus from Eastern Europe [35]. For questing D. reticulatus, the prevalence of B. burgdorferi s.l. (1.8%) was also lower than that reported for questing adult D. reticulatus from Russia (3.6%) [21]. On a regional level, the highest Borrelia prevalence in ticks of 15.9% was observed in Brest region, which seems to be in line with reports of the highest LB incidence in this region [2]. B. afzelii and B. garinii, the most prevalent Borrelia species in this study, have been previously reported to be predominant in Belarus [25]. However, this is the first report on B. burgdorferi s.s. and B. lusitaniae prevalence in ticks from Belarus.
Interestingly, the Borrelia prevalence was significantly higher in I. ricinus ticks collected from the vegetation than in those from cattle. This is surprising, as feeding adult ticks were removed during their third blood meal, whereas questing adults only fed twice. Here, however, the prevalence of feeding and questing adult ticks should be similar as cattle are not considered a competent reservoir host for B. burgdorferi s.l. due to the bactericidal activity of bovine serum which results in the complement-mediated lysis of Borrelia [36]. Sensitivity testing of our PCR on DNA extracts from feeding and questing ticks spiked with B. garinii excluded a difference in detection sensitivity. This suggests that the reduced Borrelia prevalence in feeding ticks was not an artifact due to PCR inhibition. Cattle on pastures may serve as an easier blood meal host than wild animals and thus act as a dilution factor for Borrelia prevalence in ticks. This inhibitory influence of less competent reservoir hosts on an enzootic cycle has been modeled before [37], [38].
Pathogens of the genera Anaplasma, Babesia, Bartonella, Coxiella and Francisella were exclusively found in questing ticks from Belarus with low prevalences comparable to reports from questing adult ticks from other Eastern European countries [3], [8], [9], [12], [21], [22], [39], although occasionally also higher prevalences have been reported for these pathogens [3], [21], [40]. The only study from Belarus reports a prevalence of A. phagocytophilum in questing I. ricinus from Minsk region of 4.2% [24], which is significantly lower than our findings (13.5%; 5/37; p<0.05).
These observed low prevalences in Belarusian ticks are in line with low incidence reports of these diseases in Eastern Europe. However, so far no such incidence data is available for Belarus and there are limited reports from the neighbouring countries, e.g. a single case report of arthropod-borne tularaemia in Poland has been published [41]. Serological studies from Poland and Russia reveal antibody positivities in humans of 5.1 to 11.8% for Anaplasma phagocytophilum and 2.6 to 9.0% for Babesia microti [42], [43]. Interestingly, a high seroprevalence was reported for Bartonella henselae from Poland (23.1 to 37.5%); however, this may be due to other routes of transmission like contact to infected cats rather than to tick bites.
Interestingly, hotspots of infection were discovered at sites in Minsk and Gomel region for A. phagocytophilum (12.5–17.2%), F. tularensis ssp. (5.5%) and C. burnetii (9.1%). The focality of prevalence rates suggests that zoonotic cycles of these pathogens are well established at least at these sites. Since the inhalation of contaminated aerosols (e.g. dried faeces) is an important route of transmission of F. tularensis ssp. and C. burnetii [44], [45], these sites may represent a significant threat to human health independent of tick exposure. Therefore, studies on the seroprevalence and incidence of these diseases in humans as well as the surveillance of these pathogens not only in ticks but also in reservoir hosts (e.g. members of the Leporidae for F. tularensis ssp. and of the Bovidae for C. burnetii) at the identified hotspots and neighbouring regions are warranted in order to predict and avoid outbreaks of tularaemia and Q fever.
Gomel region displayed the highest pathogen diversity in the country and is also the region with the largest numbers of ticks, suggesting that it may best reflect the infection status of ticks in Belarus at least for the low prevalent pathogens Anaplasma, Bartonella, Babesia, Coxiella and Francisella. Nevertheless, the prevalence of Borrelia infected ticks in this region seemed to be lower than in the western regions Grodno and Brest, even when only I. ricinus, a competent vector of Borrelia species, is considered. These differences may be due to habitat differences rather than the geographic location.
We found that pathogen diversity was higher in questing I. ricinus than in questing D. reticulatus ticks, suggesting that the latter species serves as reservoir for fewer pathogens than I. ricinus, at least in Belarus. Our survey revealed a high burden of tick-borne pathogens in questing and feeding I. ricinus and D. reticulatus ticks collected in different regions in Belarus, indicating a potential risk for humans and animals. The pathogenic potential of RRG and the role of D. reticulatus as its arthropod vector require further attention. Identified hotspots of infected ticks, especially when F. tularensis ssp. and C. burnetii are involved, should be included in future surveillance studies. In addition, the impact of the highly prevalent R. raoultii and other human pathogens on human health should be assessed in clinical and serological studies.
Acknowledgments
The authors would like to thank Stephanie Wolter for skilful experimental assistance.
Author Contributions
Conceived and designed the experiments: ALR SV JMH NPM GI CPM. Performed the experiments: ALR VS NPM. Analyzed the data: ALR. Contributed reagents/materials/analysis tools: SV GI CPM. Wrote the paper: ALR VS SV JMH NPM GI CPM.
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