Logger technology has revolutionised our knowledge of the behaviour and physiology of free-living animals but handling and logger attachments may have negative effects on the behaviour of the animals and their welfare. We studied southern rockhopper penguin (Eudyptes chrysocome) females during the guard stage in three consecutive breeding seasons (2008/09−2010/11) to evaluate the effects of handling and logger attachment on foraging trip duration, dive behaviour and physiological parameters. Smaller dive loggers (TDRs) were used in 2010/11 for comparison to larger GPS data loggers used in all three seasons and we included two categories of control birds: handled controls and PIT control birds that were previously marked with passive integrative transponders (PITs), but which had not been handled during this study. Increased foraging trip duration was only observed in GPS birds during 2010/11, the breeding season in which we also found GPS birds foraging further away from the colony and travelling longer distances. Compared to previous breeding seasons, 2010/11 may have been a period with less favourable environmental conditions, which would enhance the impact of logger attachments. A comparison between GPS and TDR birds showed a significant difference in dive depth frequencies with birds carrying larger GPS data loggers diving shallower. Mean and maximum dive depths were similar between GPS and TDR birds. We measured little impact of logger attachments on physiological parameters (corticosterone, protein, triglyceride levels and leucocyte counts). Overall, handling and short-term logger attachments (1–3 days) showed limited impact on the behaviour and physiology of the birds but care must be taken with the size of data loggers on diving seabirds. Increased drag may alter their diving behaviour substantially, thus constraining them in their ability to catch prey. Results obtained in this study indicate that data recorded may also not represent their normal dive behaviour.
Citation: Ludynia K, Dehnhard N, Poisbleau M, Demongin L, Masello JF, et al. (2012) Evaluating the Impact of Handling and Logger Attachment on Foraging Parameters and Physiology in Southern Rockhopper Penguins. PLoS ONE 7(11): e50429. doi:10.1371/journal.pone.0050429
Editor: Lewis George Halsey, University of Roehampton, United Kingdom
Received: June 8, 2012; Accepted: October 24, 2012; Published: November 21, 2012
Copyright: © 2012 Ludynia et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The study received financial support from the Deutsche Forschungsgemeinschaft (DFG QU148/1ff.), the Overseas Territories Environmental Programme (FAL 603) and the Falkland Islands Government Environmental Studies Budget. KL benefited from a Claude Leon Fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Logger technology has revolutionised our knowledge on the behaviour and physiology of free-living animals (see ,  for review), especially on seabirds that are usually difficult to study away from their colonies (see ). However, possible negative effects on the behaviour, physiology or survival of the birds have to be considered in any study where animals are disturbed, handled or equipped with tracking devices , –. Besides the ethical considerations regarding the animals’ welfare and their survival, changes in the animals’ behaviour induced by research activities will cause the research results to be biased and not represent the normal behaviour which the study focussed on , , . A meta-analysis by Barron et al.  showed that many studies on birds find negative device effects. For seabirds, the number of publications dealing with potential impacts of these devices on the behaviour and physiology of study animals are rather limited albeit their extensive usage . One reason for that is often the lack of comparable data from unequipped control animals , . A way of assessing the impact of logger deployment is the comparison between different device sizes and weights ,  as well as the comparison between internally and externally attached devices , .
Possible impacts of handling and logger attachments may include changes in foraging and diving behaviour, changes in time budgets as well as in physiological conditions, for example hormone levels or energy expenditure. Attachments can also alter breeding success and reduce survival rates (see ). These factors can also be linked and one might be the proximate cause of another. For example, a higher work load can lead to longer foraging trips, thus higher energy expenditure, which can result in reduced mass gain and muscular damage . However, birds can also compensate for the higher energy expenditure, for example by changing their diving behaviour .
Most of the early studies using logger technologies were conducted on penguins and more than half of the publications defined by Vandenabeele et al.  as directly assessing logger impacts deal with this group of birds. These studies have mostly focused on changes in foraging and diving behaviour. More recently, technologies allow measuring physiological parameters during foraging (see  for review), and several studies have tried to assess physiological changes that occur due to logger attachments (e.g. , , ).
Physiological changes may be caused by handling stress and the attachment of a device which can affect baseline corticosterone levels and leucocyte counts such as the ratio between heterophils (or equivalently used granulocytes) and lymphocytes and the number of leucocytes per 10,000 red blood cells ( = RBC) (e.g. , ). Additionally, higher foraging costs due to the attachment of a device could affect plasma triglycerides that are indicative of the nutritional state (e.g. , ), and plasma protein levels, which increase with physical exercise ( and literature therein). Thus, handling and carrying a device could add stress to the bird, potentially increase foraging costs and decrease foraging success. We here define stress as any disturbance of homeostasis, which is accompanied by physiological changes that aim to re-establish homeostasis . Long-term stress can lead to immune suppression and can even be fatal , . Here, we tested whether handling and the attachment of different size data loggers have a negative impact on breeding southern rockhopper penguins (Eudyptes chrysocome). We considered potential effects on the foraging behaviour and diving behaviour as well as on physiological conditions (corticosterone, protein, triglyceride levels and leucocyte counts). Using data from three consecutive breeding seasons, we further tested for differences between breeding seasons as environmental conditions may influence the level of logger effects , .
If bird handling and logger attachment had an effect in our study, we predicted to see 1) longer foraging trip duration in logger-equipped birds compared to control birds and 2) different diving behaviour in relation to logger size with shallower dive depth but more frequent dives in birds with larger devices. We further predict that 3) handled controls re-establish homeostasis and recover to pre-capture stress levels over one foraging trip, while GPS birds retain higher stress levels. We therefore expected logger-equipped birds to show an increase in baseline corticosterone levels after foraging, indicating higher foraging costs , , and an increase in granulocyte/lymphocyte ratios (G/L ratio) as well as a decrease in leucocytes per 10,000 RBC over a foraging trip , . Furthermore, we predicted 4), lower triglyceride and higher protein levels in logger-equipped birds compared to control birds after foraging, indicating decreased foraging success and increased physical exercise.
The study was approved by the Environmental Planning Department of the Falkland Islands Government (Research Licenses No: R17/2007, R12/2008, R05/2009). Potential impact of logger attachment is evaluated in the following analyses. Handling time was kept to a minimum, birds were taken out of sight of other breeding birds to keep disturbance in the colony low and the bird’s eyes were covered (see ).
The study took place at the New Island Nature Reserve (51°43′S, 61°17′W), one of the most westerly located islands of the Falkland Islands/Islas Malvinas in the Southwest Atlantic. More than 5,000 breeding pairs of southern rockhopper penguins breed at New Island . The study was carried out in three consecutive breeding seasons (2008/09 to 2010/11) during the guard stage of southern rockhopper penguins in December (thus referred to as 2008, 2009 and 2010). During this stage, female birds leave the colony for foraging and chick provisioning –, while males guard the chicks.
In all three breeding seasons, GPS-temperature-depth data loggers (GPS-TD, earth&Ocean technologies, Kiel, Germany) were used, recording the GPS positions, dive depth (using a pressure sensor) and temperature (see ,  for further details). Dimensions of the GPS data loggers were 96 mm length, 39 mm width and 26.5 mm height, thus having a cross-sectional area of 10.3 cm2 which represents about 6–10% of the birds’ cross-section (breast circumference of 105 females during Dec and Jan 2006−2009: mean 40.1 cm, range 36.0−47.0 cm). Devices weighed 75 g, thus being less than 3% of the body mass of females during guard (mean 2642 g, range 2120−3170 g; this study). The GPS data loggers were enclosed in a waterproof, hydrodynamically shaped housing and were attached to the lower back of the penguins using Tesa® tape, following Wilson et al. . Loctite® was applied to cover the tape and prevent loosening of the tape ends.
In 2010, miniature dive loggers (DST micro TD, Star Oddi, Reykjavik, Iceland) were used on additional females during guard to study their diving behaviour. Dive loggers (hereafter referred to as TDRs) were 25.4 mm long and had a diameter of 8.3 mm, thus having a cross-sectional area of 0.54 cm2, between 0.3 and 0.5% of the birds’ cross-section. TDRs weighed 3.3 g, 0.1% of the females’ body mass. TDRs were attached to the lower back using Tesa® tape and Patex® rubber glue that was peeled off after recovering the device.
Female rockhopper penguins were caught using a long pole with a metal hook to grab the birds around their legs and by hand while birds were standing next to their partners at the nest site. Sexes were distinguished by the size of the bill . After catching the bird, its head was covered and the animal was held in a tight position by one person while the other person took a blood sample and morphometric measurements (bill sizes, flipper length and mass). A passive integrative transponder (PIT; 23 mm length, Texas Instruments, USA) was inserted subcutaneously in the back of the bird between the scapulae. GPS data loggers or TDRs were attached and birds were returned to their nest sites. The deployment process, including blood sampling, tag insertion and data logger attachment, lasted between 10 and 20 minutes. Nests were checked daily to determine departure and return of the birds. An automated gateway system was installed during all breeding seasons. All birds from the study colony passed this system and for birds tagged with PITs, departure and arrival times and dates as well as body mass were recorded. Loggers were taken off when the female birds returned to their nest sites after one day at sea (in 2009 and 2010) and after three days (including 2−3 foraging trips) in 2008. Blood samples were taken in 2009 and 2010 after the return of the birds.
In 2008, ten birds were equipped with GPS data loggers of which one device was lost (logger fell off) and one device did not yield GPS information (see ). In 2009, eleven birds were equipped with GPS data loggers of which ten data loggers yielded GPS data and in 2010, 15 GPS data loggers were deployed and 13 contained useful data. Sample sizes used for individual analyses are given in the tables, as not all data sets were complete for all trips. In 2010, an additional 15 birds were deployed with TDRs.
In 2009 and 2010, ten control birds (hereafter referred to as handled control birds) were caught and handled the same way as logger-equipped birds except for the deployment of data loggers. Handled control birds allowed us to separate the impact of handling versus logger attachment. To control for handling effects, gateway readings on departure and return times were used to calculate trip durations of twenty randomly chosen birds in each breeding season. These random birds (referred to as PIT control birds) had been tagged with PITs in previous seasons and were not handled during the time of this study. Nevertheless, they cannot, strictly speaking, be considered non-handled as previous handling and PIT deployment could have long-term effects influencing their current behaviour , . PITs have been used in many bird species and have not been found to have negative effects on survival or breeding success , –, not even in small passerines . We are therefore confident that these birds constitute a reliable control category in our study.
Departure and arrival times of all birds were determined by passage through the gateway system which read the PITs of all birds included in this study. This method allowed for a better comparison of logger-equipped birds with handled and PIT control birds, as for these last two categories, trip duration could only be determined by readings from the gateway system. Only in case of missing gateway readings, GPS positions and depth/temperature data were used to determine time at sea for GPS and TDR birds. Trip durations were compared between breeding seasons and between bird categories (GPS, TDRs, handled control and PIT control birds) using linear models (LM) and linear-mixed effect models fit by REML (LME) run in R 2.13.1 (package „nlme”, , ). Individual birds were used as random factors where several trips of the same bird were considered. Given are modelled means ± standard error (SE) and minimum and maximum trip durations.
Foraging Parameters - GPS Birds
GPS data loggers were set to record GPS positions every two minutes and upon each surfacing after dives. Maximum distance to the colony was calculated for the furthest position away from the colony and distance travelled was calculated as the sum of all distances between positions. We calculated the underwater time (sum of all dives) as percentage of the trip duration (see , ).
Dive Analysis GPS Data Loggers vs. TDRs
Depth recordings were taken every second in GPS data loggers and every three seconds in TDRs. All pressure data were analysed using a custom-written Matlab script and dive parameters were calculated following Mattern et al. . For comparison between data logger types (GPS data loggers vs. TDRs in 2010), pressure data from GPS data loggers in 2010 were sub-sampled to three seconds. Dive parameters were compared between the two data logger types using LME in R, with bird identity and trip number as random factors. As dive duration was positively correlated with dive depth, depth was additionally used as a random factor when testing for differences in dive duration. Individual dives might not have been identified as such using a 3 s interval setting and longer dive durations (see results) might therefore represent a combination of several dives (see ). Post-dive periods were only considered when not greater than 180 seconds (see ) and used to calculate the dive efficiency (bottom time/(dive duration+post-dive period); following ). Dive depth distributions were compared using Chi-square test with dives in 10 m intervals (first interval 3–10 m).
Corticosterone, Protein, Triglyceride Levels and Leucocyte Counts
Blood samples in 2009 and 2010 were taken from the brachial vein within 3 minutes after capture of the bird, using a 23-gauge needle and a heparinised syringe. Blood samples were stored on ice while still in the colony, and were centrifuged thereafter. Plasma was kept frozen until the analyses took place. Blood smears were prepared in the field with blood that was taken directly from the skin puncture with a capillary. Following Ruiz et al. , one drop of blood was smeared on a glass slide and air-dried. Samples were later fixed with methanol (100%) and stained with Giemsa prior to counting.
Corticosterone concentrations (in ng/ml) were determined from blood plasma at the IZW Berlin. Plasma volumes ranged from 15–50 µl and were extracted twice with 2 ml of tert-butyl methyl ether:petrolether (30:70, v/v) for 30 min. After freezing at –80°C for 20 min, the organic phase was decanted, dried and resolved with 0.1 ml of 40% methanol. Corticosterone was quantified by using a micro-titre plate enzyme immunoassay. The extraction of different plasma volumes gave displacement curves that were parallel to the corresponding corticosterone standard curve. The calibration curves for the assay ranged from 0.2 to 100 pg/aliquot. The sensitivity of the assay was defined as two standard deviations from the signal given by the zero blank and was 0.5 pg/aliquot. The intra-assay coefficients of variation of four biological samples were 5.3, 5.6, 7.3 and 12.7% (n = 4 each). The inter-assay coefficients of variation of two biological samples were 14.9 and 20.2% (n = 8 and n = 7, respectively).
Plasma protein and triglyceride levels were determined using standard spectrophotometric test combinations (see ). We used 6 µl of plasma per determination, using the total protein reagent (n° 981826, Thermo Fisher Scientific) and protein standard sCal (n° 981831, Thermo Fisher Scientific) for calibration. For triglycerides, the procedure was repeated with the Triglycerides reagent (n° 981786, Thermo Fisher Scientific) and sCal. Absorptions were measured with a spectrometer at 540 nm wavelength each.
Blood smears (only data from 2010) were scanned with a light microscope (1000×, oil immersion) in a monolayer of blood cells. Differential leucocyte counts were accomplished along the short-axis of the slide to control for differences in the thickness of blood cells (see e.g. , ). A minimum of 100 leucocytes were counted per slide and distinguished as granulocytes (heterophils, eosinophils and basophils pooled together, see ), lymphocytes and monocytes, following Hawkey and Dennet . We calculated the G/L ratio as the ratio between granulocytes and lymphocytes, which can be used alternatively to the H/L ratio as a measurement of stress (e.g. , ). Leucocyte numbers per 10,000 RBC were calculated by counting the number of all RBC in three microscopic visual fields and multiplying the average value (i.e. average number of RBC per microscopic field) with the number of the microscopic visual fields that were scanned until reaching 100 leucocytes.
Wilcoxon and Kruskal-Wallis Signed-Rank tests (run in R) were used to test for differences in physiological parameters before and after foraging between different bird categories (GPS, TDR and handled control birds). Possible changes within bird categories before and after foraging were tested using paired Wilcoxon Signed-Rank tests.
Tip duration: Breeding Season and Device Differences
No differences in trip durations were found in PIT control birds among the three breeding seasons (F2,57 = 0.961, p = 0.389; Table 1) nor in handled controls between 2009 and 2010 (F1,18 = 0.283, p = 0.601; Table 1). GPS birds in 2010 presented longer foraging trips than in the other two seasons, including four birds with overnight trips (Table 1). Trip durations in GPS birds were not significantly different between breeding seasons (t31 = 1.906, p = 0.066). However, testing the interaction of bird category and breeding season showed a significant difference in trip duration for GPS birds in 2010 when comparing GPS and PIT control birds in all breeding seasons (t86 = 2.343, p = 0.021) as well as when comparing GPS, handled controls and PIT control birds in 2009 and 2010 (t80 = 2.153, p = 0.034). In 2010, foraging trip durations of TDR birds were comparable to PIT and handled control birds (TDR; t53 = −0.186, p = 0.853; PIT: t53 = −0.837, p = 0.407 compared to handled controls; Table 1), only GPS birds had significantly longer foraging trips than all other bird categories (t53 = 2.289, p = 0.026; Table 1, Fig. 1).
Figure 1. Trip duration of female southern rockhopper penguins from New Island.
Comparison of trip duration (in hours) in three consecutive breeding seasons between different handling and logger attachment procedures. Given are median values (line), first and third quartiles (box) and the whiskers show 1.5 times the interquartile range. Outliers are plotted as dots.doi:10.1371/journal.pone.0050429.g001
Table 1. Trip duration (in hours) for female southern rockhopper penguins during guard stage from New Island, in three consecutive breeding seasons and averaged over the three breeding seasons (modelled means ± SE, range, n = number of birds (trips)).doi:10.1371/journal.pone.0050429.t001
Foraging and Dive Parameters: Breeding Season Differences in GPS Birds
Maximum distance from the colony and distance travelled were significantly greater for GPS birds in 2010 whereas underwater time was significantly lower in 2010 than in the two previous breeding seasons (Table 2 and Fig. 2). Dive parameters of GPS birds also showed some differences between breeding seasons with significantly greater maximum dive depth in 2010 (mean max. dive depth: 2008:51.4±1.9; 2009:58.0±3.6; 2010:62.5±3.2 m; t28 = 3.439, p = 0.002) and longer mean dive durations in 2009 (2008:66.4±2.3 s; 2009:74.2±3.6 s; 2010:68.4±3.4 s; t28 = 2.190, p = 0.04).
Figure 2. Foraging parameters of southern rockhopper penguins equipped with GPS data loggers.
Maximum distance to the colony, distance travelled and underwater time (as % of trip duration) of GPS data logger-equipped females from New Island, in three consecutive breeding seasons. Stars mark significant differences for all three parameters in 2010 compared to previous breeding seasons (see Table 2).doi:10.1371/journal.pone.0050429.g002
Table 2. Foraging parameters of female rockhopper penguins equipped at New Island with GPS data loggers (modelled means ± SE, range, n = number of birds (trips), significant results in bold).doi:10.1371/journal.pone.0050429.t002
Dive Parameters - Comparison between GPS Data Loggers and TDRs
The dive depth distributions were significantly different between GPS and TDR birds (Chi-square 423.3, df = 8, p<0.001). The density distribution showed that TDR birds dived more often to deeper depths whereas more than 50% of all dives by GPS birds were within the top 20 m (Fig. 3). Nevertheless, dive depth and maximum dive depth (per bird) were not significantly different between logger types (Table 3). The deepest dives recorded were conducted by GPS birds. TDR birds dived significantly longer (Table 3) but dive durations have to be handled with care. Sub-sampling the original 1 s dive data of GPS birds to 3 s intervals led to an increase in maximum dive durations (1 s intervals: 119.0±2.9 s vs. 3 s intervals: 126.3±9.8 s) and to a decrease in numbers of dives (1 s: 5246 dives vs. 3 s: 5151 dives). GPS birds presented more dives per trip but spent less time under water in relation to the trip duration (Table 3), due to some overnight trips with reduced diving activity during the night. Dive efficiency was significantly lower in GPS birds even though post-dive intervals were significantly longer in TDR birds (Table 3).
Figure 3. Dive depth distribution of southern rockhopper penguins.
Density plot of dive depth distribution for females from New Island, carrying GPS data loggers and miniature TDR dive loggers in 2010.doi:10.1371/journal.pone.0050429.g003
Table 3. Dive parameters of female rockhopper penguins equipped at New Island in 2010 with GPS data loggers and miniature TDR dive loggers (modelled means ± SE, significant results in bold).doi:10.1371/journal.pone.0050429.t003
Corticosterone, Protein, Triglyceride Levels and Leucocyte Counts
Baseline corticosterone levels measured in GPS, TDR and handled control birds in 2010 were consistently low and were not significantly different between bird categories before (chi-square = 4.7, df = 7, p = 0.693) or after foraging (chi-square = 8, df = 8, p = 0.434; Fig. 4). Nevertheless, GPS birds showed the highest corticosterone levels of all three bird categories after foraging (GPS: 1.218±0.224; TDR: 1.051±0.128; Control: 1.106±0.250 ng/ml; Fig. 4) and corticosterone levels increased significantly in GPS birds from before to after foraging (before: 0.684±0.112 ng/ml; V = 12, p = 0.034). No significant differences were found for handled controls (before: 0.870±0.208 ng/ml; V = 8, p = 0.098) or for TDR birds (before: 0.911±0.245; V = 17, p = 0.322) between corticosterone levels measured before and after foraging.
Figure 4. Figure 4. Physiological parameters in southern rockhopper penguins.
Corticosterone, protein and triglyceride levels before and after foraging trips in females from New Island, equipped with GPS data loggers, miniature TDR dive loggers and from handled (but unequipped) control birds. The left panels represent 2009, the right panels represent 2010. The stars indicate significant differences between samples taken before and after foraging or between bird categories.doi:10.1371/journal.pone.0050429.g004
Protein levels in both breeding seasons did not change between measurements taken before and after the foraging trips within bird categories (GPS: 2009: V = 20, p = 0.492; 2010: V = 64, p = 0.502; Control: 2009: V = 35, p = 0.492; 2010: V = 46, p = 0.622; TDR: 2010: V = 60, p = 1.000; Fig. 4). In 2009, GPS birds had elevated protein levels after their foraging trips (45.88±2.54;) compared to handled control birds (Control: 38.98±1.03 g/l; W = 18.5, p = 0.019) but this was not the case in 2010 when compared to TDR and handled control birds (chi-square = 11, df = 11, p = 0.443; Fig. 4). Protein levels before their foraging trips were not significantly different between bird categories (2009: W = 40.5, p = 0.495; 2010: chi-square = 12, df = 12, p = 0.495). Protein levels in 2010 were overall higher than in 2009 (Fig. 4).
Triglyceride levels in both breeding seasons before and after foraging were not significant different between bird categories (2009: before: W = 30, p = 0.398; after: W = 61, p = 0.068; 2010: before: chi-square = 11, df = 11, p = 0.443; after: chi-square = 11, df = 11, p = 0.443) nor did we observe a change within bird categories between the two measurements (2009: handled controls: V = 26, p = 0.313; GPS: V = 16, p = 0.275; 2010: handled controls: V = 43, p = 0.791; GPS: V = 47, p = 0.761; TDR: V = 44, p = 0.389; Fig. 4). GPS birds had slightly lower triglyceride levels compared to handled control birds after foraging in 2009 (GPS: 105.57±17.18; Control: 166.73±32.89 mg/dl) but this was not found to be significant (see above).
G/L ratio and leucocytes per 10,000 RBC did not change between measurements taken before and after foraging within bird categories (G/L: GPS: V = 43, p = 0.572; TDR: V = 61, p = 0.294; handled controls: V = 27, p = 0.380; leucocytes: GPS: V = 47, p = 0.489, TDR: V = 65, p = 0.463; handled controls: V = 63, p = 0.064). Differences between bird categories were also not found to be significant (G/L: before: chi-square = 11, df = 11, p = 0.443; after: chi-square = 10.2, df = 10, p = 0.422; leucocytes: before: chi-square = 11, df = 11, p = 0.443, after: chi-square = 11, df = 11, p = 0.443; Fig. 5).
Figure 5. Blood parameters in southern rockhopper penguins.
G/L ratio and leucocytes (per 10,000 RBC) before and after foraging trips by females from New Island, equipped with GPS data loggers, miniature TDR dive loggers and from handled control birds in 2010.doi:10.1371/journal.pone.0050429.g005
In our study, most parameters showed no or little differences between the different bird categories. However, some parameters were significantly different for GPS birds, thus indicating negative impacts of GPS logger attachments. Foraging trip durations were similar between PIT control birds, handled control birds and logger-equipped birds in most breeding seasons. However, foraging trip duration was significantly longer for GPS birds in 2010 compared to other birds in the same breeding season and to GPS birds in previous breeding seasons. Rockhopper penguins during guard in 2010 also foraged further away from the colony, travelled larger distances and spent less time underwater compared to birds in the previous two breeding seasons. Logger and flipper band effects can become more obvious in years of unfavourable environmental conditions , , as birds are less capable to find food and thus to compensate for higher energy expenditure that they might experience while carrying the device . However, Pietz et al.  states that transmitter effects may only be detectable during intermediate levels of environmental stress. During favourable years, even handicapped birds will be able to find enough food whereas during unfavourable years, all birds will show signs of stress . Unfortunately, we have no direct measure of environmental conditions during the three breeding seasons of this study and it is therefore difficult to determine whether birds in 2010 were already compromised by unfavourable environmental conditions and thus more susceptible to logger effects than during the other breeding seasons. Breeding parameters are commonly used as indicators for food availability (see ,  for overview) but breeding success, chick mass, average mass at arrival and average mass of females during the guard stage showed no differences between breeding seasons in our study (authors unpubl. data). However, a higher mortality between the seasons 2009/10 and 2010/11 was observed (authors unpubl. data), thus conditions might have been unfavourable before the onset of breeding.
As logger size might play an important role in the effects caused by the devices , , , we tested the effect of logger size using two different types of devices in 2010. Logger size did influence the diving behaviour of birds in our study as birds deployed with larger GPS data loggers presented more shallow dives compared to birds carrying miniature TDRs. Changes in dive behaviour are the most commonly observed effects of logger attachments in several penguin species (see Table 4 for overview). Little (Eudyptula minor) and Adélie penguins (Pygoscelis adeliae) dived shallower when carrying larger devices compared to birds with smaller loggers attached , . Changes in dive depth frequencies, as observed in southern rockhopper penguins in our study, were also observed by Ropert-Coudert et al.  in king penguins (Aptenodytes patagonicus) carrying externally attached devices. According to Wilson , the maximum dive depths of Adélie and gentoo penguins (Pygoscelis papua) were correlated to the cross-sectional area of the devices, following the equation: max depth (m) = 166.5 - (0.08 × cross-sectional area in mm2). Applying this equation to our data, GPS birds should have reached only half the maximum depth of TDR birds. However, birds carrying smaller TDRs had only slightly greater maximum and mean dive depths and the deepest dives recorded were by GPS birds.
Table 4. Overview of logger impacts on different penguin species.doi:10.1371/journal.pone.0050429.t004
Besides alterations of foraging and diving behaviour, handling and logger attachments can have effects on physiological parameters , –, . Even birds that seem to be calm can be stressed while being handled or disturbed , ,  and the pure presence of a human close to a bird can cause alterations in stress hormones . In our study, little impact was measured in physiological parameters due to logger attachments. GPS birds did show elevated protein levels in 2009 though when compared to handled control birds after foraging. We also found a significant increase in corticosterone levels in 2010 from before to after foraging in GPS birds, although GPS birds had the lowest median corticosterone level in 2010 after foraging. Significant results, however, could be influenced by multiple testing as we conducted several tests on the same data set . The large variation in baseline corticosterone values observed in our study might be due to individual differences in behaviour, as, for example, more aggressive birds show higher baseline corticosterone values , . In king penguins, corticosterone levels were not found to be related to food acquisition  but several studies have found baseline corticosterone levels to be related to food-related stress in other seabirds (: Wilson’s storm-petrels (Oceanites oceanicus), : common murres (Uria aalge)). Changes in protein and triglyceride levels can also be attributed to food intake and the nutritional state of the animal , , . Additionally, protein levels are known to increase under physical exercise . However, Navarro et al.  found no significant differences in plasma protein and triglycerides of handicapped compared to non-handicapped Cory’s shearwaters (Calonectris diomedea) and no significant differences in plasma triglyceride and uric acid (an indicator for protein breakdown) were found in Adélie penguins even though logger-equipped birds did present longer foraging trips . Similarly, longer foraging trips and less foraging success did not lead to changes in physiological parameters in our study.
While the number of leucocytes per 10,000 RBC so far has been mainly shown to decline during handling stress (e.g. ), the G/L ratio in southern rockhopper penguins is related to body condition, and higher values indicated stress during fasting periods . In fact, other studies showed that the equivalently used H/L ratio could be more sensitive to certain environmental and chronic stressors than changes in baseline corticosterone , , . Nevertheless, our results show no increase in G/L ratios and no decrease in leucocyte numbers in control or logger equipped birds. Thus, potentially stressful handling and logger attachment, as detected by elevated cortiscosterone and protein levels, seem not to have affected leucocyte counts. Similarly, Jakubas et al.  did not find differences in H/L ratios in little auks (Alle alle) breeding under different oceanographic conditions. Birds compensated for lower food abundance and changes in H/L ratio might only become detectable once food sources are below a certain threshold level .
Overall, effects of handling and logger attachment seem to be rather small when considering foraging behaviour and physiological parameters although GPS devices did influence the diving behaviour and foraging success in one of the breeding seasons, likely as a consequence of the GPS devices’ larger cross-sectional area, and might have caused birds to forage longer during one of the breeding seasons. Many studies have found no effect of logger attachments and flipper bands during single seasons or during short-term deployments (, , , but see , ) but long-term studies have shown increased corticosterone levels after year-long deployments with geolocators  and reduced survival in birds equipped with flipper bands . In our study, birds were only deployed for one foraging trip in 2009 and 2010, usually not lasting longer than one day, and for three days in 2008 (up to three foraging trips; ), thus impact of logger attachment was to be expected lower than in studies where birds carried devices for several weeks , . Short deployments might not be felt as chronic stress by the birds, thus explaining the little effects on physiological parameters . Short term deployments of PTTs on wandering albatrosses (Diomedea exulans) have not been found to negatively influence demographic parameters (, but see ) and resighting rates of previously logger-equipped rockhopper penguins in our study were similar to the overall resighting rate of PIT tagged birds (authors unpubl. data).
Studies on flying birds indicate that devices should not exceed 3% of the body mass (, but see , ) for further considerations) but for swimming and diving birds, the cross-sectional dimensions seem to be more important than mass , , . Even though GPS data loggers weighed less than 3% of the rockhopper body masses, they had relatively large cross-sectional areas compared to miniature TDRs. Significant differences in the dive depth frequencies between GPS and TDR birds can be most probably related to the increased drag due to the larger size of our GPS devices . Besides potentially causing higher energy expenditure for the birds , it also reveals that dive parameters measured by externally (and possibly also internally; see ) attached devices might not represent the birds’ normal diving behaviour, thus not representing “real” dive data, although this could be derived through extrapolation (see ). Longer foraging trip durations, foraging further away from the colony and increased baseline corticosterone levels were only observed in 2010. These factors might be linked as birds foraging further away from the island would spend more time at sea and possibly have higher foraging costs, which could explain the high corticosterone levels. Differences observed in 2010 could be related to unfavourable environmental conditions, which might have also further enhanced the differences found in the dive parameters between GPS and TDR birds.
One-day (or very short) logger attachments have little effects on the overall breeding activity of the birds even though they may cause lower energetic gain during the trip . We are therefore confident that the use of externally attached devices on southern rockhopper penguins in our study has not jeopardised the survival of the birds but device sizes should be reduced in future studies to eliminate the effects we did find on certain foraging, dive and physiological parameters.
We are grateful to the New Island Conservation Trust for permission to work on the island and for providing logistic support. We would like to thank Ian, Maria and Georgina Strange, Charles Swift and George Guille for their contributions to the logistics at New Island, Thomas Mattern for providing a script to calculate dive parameters, Miguel Lecoq for reading tags, Hendrika (Riek) van Noordwijk and Gabriele Schafheitle for help in the field and lab, respectively, and Martin Dehnhard (IWZ Berlin) for corticosterone measurements. We would like to thank Martin Wikelski for supplying the data loggers and three anonymous reviewers and Lewis George Halsey for constructive comments on an earlier version of the manuscript. This study was approved by the Falkland Islands Government (Environmental Planning Office).
Conceived and designed the experiments: KL ND MP JFM PQ. Performed the experiments: KL ND MP LD JFM PQ. Analyzed the data: KL ND. Contributed reagents/materials/analysis tools: KL ND MP PQ. Wrote the paper: KL ND MP LD JFM PQ.
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