Prior to the evolution of DNA-based organisms on earth over 3.5 billion years ago it is hypothesized that RNA was the primary genetic molecule. Before RNA-based organisms arose, peptide nucleic acids may have been used to transmit genetic information by the earliest forms of life on earth. We discovered that cyanobacteria produce N-(2-aminoethyl)glycine (AEG), a backbone for peptide nucleic acids. We detected AEG in axenic strains of cyanobacteria with an average concentration of 1 µg/g. We also detected AEG in environmental samples of cyanobacteria as both a free or weakly bound molecule and a tightly bound form released by acid hydrolysis, at concentrations ranging from not detected to 34 µg/g. The production of AEG by diverse taxa of cyanobacteria suggests that AEG may be a primitive feature which arose early in the evolution of life on earth.
Citation: Banack SA, Metcalf JS, Jiang L, Craighead D, Ilag LL, et al. (2012) Cyanobacteria Produce N-(2-Aminoethyl)Glycine, a Backbone for Peptide Nucleic Acids Which May Have Been the First Genetic Molecules for Life on Earth. PLoS ONE 7(11): e49043. doi:10.1371/journal.pone.0049043
Editor: Ahmed Moustafa, American University in Cairo, Egypt
Received: July 13, 2012; Accepted: October 5, 2012; Published: November 7, 2012
Copyright: © 2012 Banack et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The authors have no support or funding to report.
Competing interests: The authors have declared that no competing interests exist.
It has been hypothesized that RNA was the primary molecule for conveyance of genetic information by life on earth prior to the evolution of DNA over 3.5 billion years ago , . However, the nature of primitive genetic systems before the evolution of RNA-based organisms is unclear. Polymers of N-(2-aminoethyl)glycine (AEG, Fig. 1), have been hypothesized as possible backbones of peptide nucleic acids (PNAs) that facilitated transmission of genetic information in the pre-RNA world , . PNAs based on AEG have been synthesized and studied –.
Figure 1. AEG [N-(2-aminoethyl)glycine] is a small molecule which when polymerized can form a peptide nucleic acid backbone.
A, The AEG monomer. B, its proposed role as a peptide nucleic acid showing three AEG molecules each connected to a base by an acetyl linkage. C, Predicted fragmentation pattern of AQC derivatized AEG (m/z 459) following collision-induced dissociation to produce daughter ions of m/z 171, 214, 289 and 119. Predicted structures were produced using High Chem Mass Frontier 5.1 software (High Chem Ltd., Slovak Republic).doi:10.1371/journal.pone.0049043.g001
Cyanobacteria, photosynthetic Gram-negative bacteria, arose early in the earth’s history based on fossils from the Early Archean Apex Chert of Western Australia dating to 3.5 billion years ago . Cyanobacteria were major contributors to the oxygenation of the earth’s atmosphere, and picoplankton such as Prochlorococcus and Synechococcus continue to play an important role in the global carbon balance . Cyanobacteria are cosmopolitan, with some taxa occupying extreme habitats such as geothermal pools, hypersaline waters, or polar environments.
To determine if cyanobacteria produce AEG, we analyzed axenic cultures of cyanobacteria from the Pasteur Culture Collection (PCC) as well as environmental collections of cyanobacteria using triple quadrupole mass spectrometry (LC-MS/MS). Analyses were independently conducted at the Institute for Ethnomedicine in Jackson Hole, USA and at the Department of Analytical Chemistry of Stockholm University, Sweden.
We detected AEG as a free or weakly bound molecule in eight axenic PCC strains (Table 1; Fig. 2), which included both nitrogen-fixing and non-nitrogen fixing cyanobacteria from all five morphological cyanobacterial groups . The total concentrations of free AEG and AEG liberated as a result of TCA extraction in the PCC strains ranged between 281 and 1717 ng/g. We also detected AEG in 15 different environmental samples of cyanobacteria we collected from diverse habitats around the world ranging from freshwater ponds in the deserts of Mongolia to marine samples from Qatar and river samples from Japan (Table 1; Fig. 2). The concentrations of AEG detected in environmental samples were generally higher than for the axenic samples, with concentrations ranging between not detected and 34 µg/g. After the removal of free and weakly bound AEG with a TCA extraction step, we hydrolyzed the precipitate, and detected AEG as a bound form in five environmental samples (Table 1). We could not detect AEG in blank BG11 media, before or after hydrolysis.
Figure 2. Cyanobacteria produce AEG [N-(2-aminoethyl)glycine], both in axenic PCC strains and environmental samples.
Using triple quadrupole LC-MS/MS analysis, AEG was identified using a precursor ion m/z 459 and selective reaction monitoring of four transitions m/z 459 to m/z 289 (top pane), m/z 214 (second pane), m/z 171 (third pane), and m/z 119 (bottom pane). a, AEG was detected as a free or weakly bound compound in axenic Nostoc PCC 7120. b, A synthetic AEG standard. c, AEG in an extract of bound compounds from an environmental cyanobacterial sample collected at Benson Marina, Logan, Utah, USA.doi:10.1371/journal.pone.0049043.g002
Included in our analysis were two strains (Nostoc PCC 7120 and Synechocystis PCC 6803) that have had their complete genomes sequenced , . The production of AEG by both strains, which have only a 37% sequence similarity , suggests that the cyanobacterial ability to produce AEG is highly conserved. This, coupled with our discovery of AEG in all five morphological sections of cyanobacteria  suggests that the production of AEG may be a primitive feature within cyanobacteria. We are confident of our detection of AEG in cyanobacteria since LC-MS/MS is well-suited to the detection of small molecules in complex matrices  and has been shown to distinguish synthetic AEG from its structural isomers .
It is possible that AEG may occur in higher trophic levels in food chains based on cyanobacteria, since cyanobacteria are primary producers in many aquatic and some terrestrial ecosystems. The presence of AEG as a bound form in environmental samples suggests that biomagnification is possible as occurs for some of its isomers , .
The metabolic function of AEG in extant species of cyanobacteria and its potential evolutionary significance is unknown, although we do note that PNAs have been investigated by the pharmaceutical industry as possible gene silencers , . Recent research shows synthetic xeno-nucleic acid to be susceptible to evolutionary change , . It is tantalizing to hypothesize that the presence of AEG in cyanobacteria may be an echo of the pre-RNA world.
Materials and Methods
Axenic cyanobacterial strains were obtained from The Pasteur Culture Collection of Cyanobacteria, Paris (PCC) and grown for two months according to PCC recommendations. When sufficient biomass was attained, strains were lyophilized and free and weakly bound amino acids were extracted with TCA . The remaining pellet was then hydrolyzed in 6 M HCl . Extracts were derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) and analyzed by LC-MS/MS . Blank BG11 media containing nitrate was tested as a control. Environmental samples were similarly extracted and analyzed ,  with the exception that several samples were hydrolyzed directly without TCA extraction, one sample was hydrolyzed in 6 M NaOH (Table 1), and the samples analyzed by Stockholm University were extracted using 10% TCA. The TCA extract was also hydrolyzed for all the Utah samples, one of which was positive for AEG (Table 1). Identification of AEG was based upon (a) the presence of the parent ion m/z 459; (b) retention time; (c) presence of product ions from collision-induced dissociation (m/z 171 quantifier ion; m/z 289, m/z 214, m/z 119 qualifier ions [cf. Ref 16 Fig. 1A]); and (d) ratios of qualifier ions relative to the quantifier ion. All samples were compared with an authenticated AEG standard (A1153 TCI America). Separation of AEG from its isomers β-N-methylamino-L-alanine (BMAA) and 2,4-diaminobutyric acid (2,4-DAB) was assessed using authenticated standards and culture extracts spiked with AEG and these standards, which showed a minimum separation of 0.15 min per isomer. The standard curve was prepared using six AEG concentrations (n ≥3) covering three orders of magnitude (7.4–740 nM AEG, r2 = 99.9%). The limits of detection (LOD) and the limits of quantification (LOQ) for AEG were 7.4 nM and 37 nM, respectively.
Conceived and designed the experiments: SAB JSM PAC. Performed the experiments: SAB JSM LJ LLI. Analyzed the data: SAB JSM LJ LLI. Contributed reagents/materials/analysis tools: SAB JSM DC PAC. Wrote the paper: SAB JSM LLI PAC.
- 1. Cech T (1986) A model for the RNA-catalyzed replication of RNA. Proc Natl Acad Sci USA 83: 4360–4363.
- 2. Joyce GF (2002) The antiquity of RNA based evolution. Nature 418: 214–221.
- 3. Nelson KE, Levy M, Miller SL (2000) Peptide nucleic acids rather than RNA may have been the first genetic molecule. Proc Natl Acad Sci USA 97: 3868–3871.
- 4. Nielsen PE (1993) Peptide nucleic acid (PNA): A model structure for the primordial genetic material? Origins Life Evol Biosphere 23: 323–327.
- 5. Nielsen PE, Egholm M, Berg RH, Buchardt O (1991) Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide. Science 254: 1497–1500.
- 6. Egholm M, Buchardt O, Nielsen PE, Berg RH (1992) Peptide nucleic acids (PNA). Oligonucleotide analogues with an achiral peptide backbone. J Am Chem Soc 114: 1895–1897.
- 7. Egholm M, Nielsen PE, Buchardt O, Berg RH (1992) Recognition of guanine and adenine in DNA by cytosine and thymine containing peptide nucleic acids (PNA). J Am Chem Soc 114: 9677–9678.
- 8. Dueholm KL, Egholm M, Behrens C, Christensen L, Hansen HF, et al. (1994) Synthesis of peptide nucleic acid monomers containing the four natural nucleobases: thymine, cytosine, adenine, and guanine and their oligomerization. J Org Chem 59: 5767–5773.
- 9. Christensen L, Fitzpatrick R, Gildea B, Petersen KH, Hansen HF, et al. (1995) Solid-phase synthesis of peptide nucleic acids. J Peptide Science 3: 175–183.
- 10. Schopf JW (1993) Microfossils of the early archean apex chert: New evidence of the antiquity of life. Science 260: 640–646.
- 11. Hugler M, Sievert SM (2011) Beyond the Calvin cycle: Autotrophic carbon fixation in the ocean. Annu Rev Mar Sci 3: 261–289.
- 12. Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Micro 111: 1–61.
- 13. Kaneko T, Tabata S (1997) Complete genome structure of the unicellular cyanobacterium Synechocystis sp. PCC 6803. Plant Cell Physiol 38: 1171–1176.
- 14. Kaneko T, Nakamura Y, Wolk CP, Kuritz T, Sasamoto S, et al. (2001) Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120. DNA Res 8: 205–213.
- 15. Domon B, Aebersold R (2006) Mass spectrometry and protein analysis. Science 312: 212–217.
- 16. Banack SA, Metcalf JS, Spáčil Z, Downing TG, Downing S, et al. (2011) Distinguishing the cyanobacterial neurotoxin β-N-methylamino-L-alanine (BMAA) in the presence of other diamino acids. Toxicon 57: 730–738.
- 17. Cox PA, Banack SA, Murch SJ (2003) Biomagnification of cyanobacterial neurotoxins and neurodegenerative disease among the Chamorro people of Guam. Proc Natl Acad Sci USA 100: 13380–13383.
- 18. Murch SJ, Cox PA, Banack SA (2004) A mechanism for slow release of biomagnified cyanobacterial neurotoxins and neurodegenerative disease in Guam. Proc Natl Acad Sci USA 101: 12228–12231.
- 19. Wagner E (2008) The silent (R)evolution of polymeric nucleic acid therapeutics. Pharm Res 25: 2920–2923.
- 20. Ray A, Norden B (2000) Peptide nucleic acid (PNA): Its medical and biotechnical applications and promise for the future. FASEB J 14: 1041–1060.
- 21. Pinheiro VB, Taylor AI, Cozens C, Abramov M, Renders M, et al. (2012) Synthetic genetic polymers capable of heredity and evolution. Science 336: 341–344.
- 22. Joyce GF (2012) Toward an alternative biology. Science 336: 307–308.
- 23. Banack SA, Johnson HE, Cheng R, Cox PA (2007) Production of the neurotoxin BMAA by a marine cyanobacterium. Mar Drugs 5: 180–196.
- 24. Jiang L, Aigret B, De Borggraeve WM, Spacil Z, Ilag LL (2012) Selective LC-MS/MS method for the identification of BMAA from its isomers in biological samples. Anal Bioanal Chem 6: 1719–1730.