Expansions of the polyglutamine (polyQ) domain (≥34) in Ataxin-2 (ATXN2) are the primary cause of spinocerebellar ataxia type 2 (SCA2). Recent studies reported that intermediate-length (27–33) expansions increase the risk of Amyotrophic Lateral Sclerosis (ALS) in 1–4% of cases in diverse populations. This study investigates the Turkish population with respect to ALS risk, genotyping 158 sporadic, 78 familial patients and 420 neurologically healthy controls. We re-assessed the effect of ATXN2 expansions and extended the analysis for the first time to cover the ATXN2 locus with 18 Single Nucleotide Polymorphisms (SNPs) and their haplotypes. In accordance with other studies, our results confirmed that 31–32 polyQ repeats in the ATXN2 gene are associated with risk of developing ALS in 1.7% of the Turkish ALS cohort (p = 0.0172). Additionally, a significant association of a 136 kb haplotype block across the ATXN2 and SH2B3 genes was found in 19.4% of a subset of our ALS cohort and in 10.1% of the controls (p = 0.0057, OR: 2.23). ATXN2 and SH2B3 encode proteins that both interact with growth receptor tyrosine kinases. Our novel observations suggest that genotyping of SNPs at this locus may be useful for the study of ALS risk in a high percentage of individuals and that ATXN2 and SH2B3 variants may interact in modulating the disease pathway.
Citation: Lahut S, Ömür Ö, Uyan Ö, Ağım ZS, Özoğuz A, et al. (2012) ATXN2 and Its Neighbouring Gene SH2B3 Are Associated with Increased ALS Risk in the Turkish Population. PLoS ONE 7(8): e42956. doi:10.1371/journal.pone.0042956
Editor: Reiner Albert Veitia, Institut Jacques Monod, France
Received: April 6, 2012; Accepted: July 16, 2012; Published: August 20, 2012
Copyright: © Lahut et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: Funding provided by Suna and Inan Kirac Foundation (http://en.kiraca.com.tr/Corp-Responsibility/detail_kiracfoundation.aspx?SectionID=C2%2bWE3CIWXkxHtpw8lqgFA%3d%3d&ContentId=faQeVeZudHFv1A4ACnP5uA%3d%3d), Bogazici University Research Funds (http://www.arastirma.boun.edu.tr/arastirma.php?a_l=tr&p=130) and Deutsche Forschungsgemeinschaft(AU96/13-1) (http://www.dfg.de/index.jsp). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
ALS is a late-onset, rapidly progressive and devastating neurodegenerative disorder, which is generally associated with selective degeneration of both upper and lower motor neurons (MNs) in the brain, brainstem and spinal cord. Ten per cent of all ALS cases are inherited and referred to as familial ALS (fALS); the remaining 90% are sporadic (sALS) . Although the mechanisms causing ALS are not well understood yet, several genes have been linked to the disease. Among these, SOD1 involved in oxidative stress, TARDBP and FUS implied in RNA-processing , , , , , , and the two recently identified UBQLN2 and C9ORF72 genes are the most prominent causes of fALS , , . Recently, genome wide association studies (GWAS) have identified SNPs that are associated with sALS, underscoring the importance of investigating common genetic variations , , .
In addition to rare mutations and common SNPs, a recent publication reported that ATXN2 dysfunction influences the TDP-43-dependent toxicity seen in ALS and that the intermediate-length expansions to 27–33 triplets in the ATXN2 polyglutamine (polyQ) region act as ALS risk factors in 4.7% of North American patients . All follow-up studies confirmed the association of the ATXN2 polyQ expansions with ALS risk in various ethnic populations, including North Americans, Europeans, French-Canadians and Chinese , , , , , , . Further, North-American studies investigated the specificity of the association between ATXN2 and ALS. Ross et al. demonstrated that intermediate-length polyQ expansions are associated with neither Alzheimer’s nor idiopathic Parkinson’s diseases, but with ALS and the Parkinson-plus entity progressive supranuclear palsy . In addition, Lee et al. as well as Gispert et al. reported that among several other polyQ neurodegenerative disease proteins, only ATXN2 is associated with ALS risk , , suggesting that the physiological functions of ataxin-2 in RNA processing and/or receptor tyrosine kinase endocytosis are relevant , , , , . The polyQ expansion is thought to convey a gain-of-function (GOF) effect on the ataxin-2 protein and to provoke insolubility and aggregation of ataxin-2 with its interacting proteins , , . However, a loss-of-function of ataxin-2, in addition to its GOF, cannot be excluded, since both mechanisms seem to be important modulators of disease manifestation in several neurodegenerative diseases .
ATXN2 usually contains a repeat structure with 22 or 23 triplets coding for glutamine and the (CAG)8CAA(CAG)4CAA(CAG)8 sequence; expansion of this domain to a size ≥34 triplets with a pure CAG sequence primarily causes autosomal dominant SCA2 , while ATXN2 expansions with CAA interruptions were observed as the cause of Levo-dopa responsive Parkinson’s disease . ATXN2 expansions associated with ALS were reported by Corrado et al. to be interrupted by at least one CAA triplet , Yu et al. identified ATXN2 expansions in 40 ALS patients to be always interrupted by CAA triplets, and defined a haplotype of two ATXN2 SNPs (rs695871 and rs695872) in common between most cases with 3 CAA interruptions and another haplotype in common between most cases with 1–3 CAA interruptions , .
This study now aims to investigate the association of the ATXN2 chromosomal region with ALS risk in the Turkish population, considering not only the polyQ repeats, but also common SNPs and haplotype patterns.
Materials and Methods
The Ethics Committee of Boğaziçi University approved the use of patient samples for this study. Written informed consent forms were obtained from all patients. Control samples were collected anonymously.
PolyQ Expansion Analyses
A total of 236 Turkish ALS patients (158 sALS and 78 fALS) matching El Escorial Criteria  were referred to our center from several hospitals throughout Turkey. Fifteen of these patients had already a defined mutation in one of the genes responsible for ALS and these were also included in the study, in addition to 420 Turkish healthy controls without any known history of neurological disorders. These control samples were collected from the Microbiology Department of Haydarpaşa State Hospital in Istanbul. The mean ages of onset of sALS and fALS patients were 48.9 (range: 24–79) and 34.3 (range: 8–80) years, respectively. The average age of the control group was 63.8 (range: 38–97). Male to female ratios were 3:2 for sALS, 1:1 for fALS and 1:1 for controls. DNA was extracted from peripheral blood cells of patients and controls, using the MagNAPure Compact (Roche) DNA isolation systems.
The ATXN2 triplet repeat was amplified from DNA samples of patients and healthy controls, using polymerase chain reaction (PCR) with the forward primer 5′- GGG CCC CTC ACC ATG TCG -3′ and the FAM labeled reverse primer 5′−/56-FAM/CGG GCT TGC GGA CAT TGG -3′. PCR cycles included 5 min. at 95°C, 30 cycles (1 min. at 95°C, 1 min. at 55.7°C, 1 min. and 30 sec. at 72°C) and 5 min. at 72°C. Repeat sizes were determined by GeneScan Analysis (Macrogen Inc., Seoul, Korea) and evaluated independently by two authors (SL and ÖÖ). The reproducibility of the GeneScan analyses was validated via repeating 25 samples among 236 ALS cases and 45 samples out of 420 controls, corresponding to ~10% of both cohorts. A SCA2 positive individual with 41 ATXN2 repeat expansion size was used as an internal control in both PCR amplifications and GeneScan analyses. ALS patients with an ATXN2 expansion were further subjected to DNA sequencing, to assess the presence of CAA interruptions (RefGen Inc, Ankara, Turkey).
Fisher’s exact test was applied to evaluate the genetic association of ATXN2 expansion sizes with ALS risk, under both the allelic and genotypic models.
SNP and Haplotype Association Analyses
In our independent GWA study (unpublished data) performed earlier, we investigated 733,202 SNPs in 116 out of the above 158 Turkish sALS patients and 109 age- and sex- matched neurologically healthy individuals, using the Illumina HumanOmniExpress SNP array. To examine the association of ATXN2 locus variants with ALS risk in the Turkish cohort under study, we extracted 250 kb genotype data, comprising the ATXN2 locus and the surrounding 50 kb (25 kb from 5′ and 3′ ends) using PLINK software (http://pngu.mgh.harvard.edu/purcell/plink/) . The SNPs with a Hardy-Weinberg equilibrium (HWE) of >0.05 and a minor allele frequency (MAF) of >0.01 were included. Haploview 4.2 software was also used to visualize the haplotype blocks in the ATXN2 and the neighbouring SH2B3 genes (http://www.broad.mit.edu/haploview/haploview) . The haplotype blocks, including SNPs in linkage disequilibrium (LD), were determined via the Gabriel method (CI: 0.98–0.7) . We performed 1000 permutation tests to generate empirical p-values.
ATXN2 Expansion Analyses
We investigated the ATXN2 expansion sizes of 236 ALS patients and 420 healthy controls from Turkey, using GeneScan analysis. The lengths of the ATXN2 repeat alleles varied from 13 to 32 units in ALS patients and from 15 to 29 units in controls (Figure 1). The commonly found 22 repeat allele accounted for approximately 92% of both ALS and control populations. None of the 15 ALS patients, carrying various mutations in different ALS disease genes (SOD1, FUS, UBQLN2, OPTN, SPG11 and PLEKHG5) showed expansions in ATXN2 (p = 0.78) (Table S1). In the allelic model, the MAF of the 31 allele was 0.21% (1/472 alleles) in cases, whereas this allele was absent in controls. The MAF of the 32 allele was 0.63% (3/472 alleles) in cases and again this allele was absent in controls. Fisher’s exact test detected a significant difference for alleles coding a polyQ size >30 between cases and controls (p = 0.016). Also in the genotypic model, the heterozygous presence of a polyQ size >30 showed statistically significant association with ALS risk according to Fisher’s exact test (p = 0.0172) (Table 1). Sequencing of the ATXN2 expansions of the four ALS patients with sizes 31 and 32 showed that three patients had a single CAA interruption within the CAG repeat, while the remaining patient has a pure CAG tract. Clinical information and the sequence data of these four ALS patients are shown in Table 2.
Figure 1. Distribution of ATXN2 repeat sizes, represented as polyQ triplet numbers.
Turkish ALS patients are represented in red and healthy controls in blue bars; numbers of the individuals having the relevant alleles are shown on the top of each bar.doi:10.1371/journal.pone.0042956.g001
Table 1. The frequency of ATXN2 expansion sizes in ALS and controls (in the genotypic model).doi:10.1371/journal.pone.0042956.t001
Table 2. Clinical characteristics of four Turkish ALS patients with ATXN2 expansions.doi:10.1371/journal.pone.0042956.t002
ATXN2 Locus SNP and Haplotype Association Analyses
We investigated a 250 kb region on chromosome 12q in 116 out of the above 158 Turkish sALS patients, including two of the four ALS patients with ATXN2 expansions. Ten of 28 SNPs were excluded due to low HWE and MAF scores. In single marker analysis, none of the SNPs within the ATXN2 gene by itself showed any significant association with ALS risk, but a trend towards association was observed for the SNP rs2239194 within the SH2B3 gene (p = 0.063) (Table S2). On the other hand, haplotype analysis of the region, using the Haploview program, demonstrated a strong association for a 136 kb 15-SNP haplotype block (including rs2239194), which contains both the ATXN2 and SH2B3 loci. One haplotype (GGGGAAGAGAAGGAC, MAF = 0.149, Fcases = 0.194, Fcontrols = 0.101) had a significant p-value of 0.0057 and correlated with an increased ALS risk (OR: 2.23). This risk haplotype was observed in heterozygous state in both Turkish ALS patients with ATXN2 expansions, which were part of the GWA genotyping study. Using permutation analysis, which eliminates false positive data after multiple testing more effectively than Bonferroni corrections , this haplotype-risk association retained its statistical significance (p = 0.02) after 1000 permutations (Figure 2).
Figure 2. Haplotype block analysis of ATXN2 locus on chromosome 12q (110,300,000–110,550,000).
Genotyped SNPs located in 250 kb region were analyzed by Haploview 4.2 in order to identify haplotype blocks with ALS risk. A 136 kb large haplotype block, highlighted in red, was observed in the analysis. The frequencies of haplotypes defined in this haplotype block, the p-values and permutation p-values are shown.doi:10.1371/journal.pone.0042956.g002
The first analysis of Turkish ALS patients regarding ATXN2 confirms its role as a risk factor. More importantly, this study identifies a common risk haplotype for ALS, containing the ATXN2 and its neighbouring SH2B3 gene.
The initial observation that ataxin-2 acts as a modifier protein of TDP-43 overexpression toxicity as a model of ALS risk was consistent from Saccharomyces cerevisiae via Drosophila melanogaster to Homo sapiens , and the role of ATXN2 intermediate expansions as ALS risk factor was reproduced in every follow-up study , , , , , , , , , , . This effect is now also validated in the Turkish population. We did not observe a specific geographic distribution within Turkey or a particular phenotype effect. The effect was limited to the expansion sizes 31 and 32 in this study, while the association was not significant for intermediate expansion alleles of size <30, and the very rare occurrence of large expansion alleles in ALS was not detected, as would be expected from the limited number of patients available. CAA interruptions were detected in the expanded alleles of three out of four ALS patients, but a pure CAG expansion was detected in one ALS patient, suggesting that CAA interruptions are not a prerequisite for ALS manifestation.
In our study, novel evidence indicates that ALS risk is impacted by a 15-SNP haplotype block in linkage disequilibrium across the genes ATXN2 and its downstream neighbour SH2B3. Haplotype association became more significant when SNPs from both the ATXN2 and SH2B3 genes were included; this suggests a role of the SH2B3 gene in ALS risk. The SH2B3 protein, also known as LNK, is a member of the SH2B (1–3) adaptor protein family. They all contain Src Homology 2 (SH2) domains, pleckstrin motifs and proline-rich regions. Thus, they can bind to phosphatidylinositol-lipid containing membranes and to the phosphorylated tyrosine residues, e.g. of receptor tyrosine kinases, modulating the signal transduction that controls proliferation and growth. They exert strong effects in hematopoiesis , , , . Preliminary studies in the nervous system indicate that SH2B3, expressed in cortical neurons from embryonic stages, competes with the other family members (SH2B1 and SH2B2) and inhibits the NGF-induced differentiation of PC12 cells reducing the neurite outgrowth of cortical neurons, through binding of its SH2 domain to the NGF receptor and repressing the PI3K pathway . Previously, a SNP (rs3184504) in the SH2B3 gene was found associated with multiple sclerosis . ATXN2 has similar features with SH2B3 since it contains proline-rich regions that interact with Src Homology 3 domains, it associates with receptor tyrosine kinases and modulates the signaling control of growth , .
This first analysis of Turkish ALS patients on ATXN2, not only confirmed its role as a risk factor in rare cases with intermediate polyQ expansions, but also revealed novel evidence that SNPs across the ATXN2/SH2B3 genomic locus may modulate risk in a substantial fraction of ALS patients. These data need to be validated in large and independent populations. In the light of these findings, our results implicate a genetic (and a possible biological) interaction between ATXN2 and SH2B3 genes, therefore we propose that it will be useful to investigate genetic variations in this genomic region of ALS patients.
Data on Turkish ALS patients with previously identified mutations.
Association Analysis of 18 SNPs across 250 kb at the ATXN2 Locus.
We would like to thank our patients and their families for their cooperation. We thank Aslı Gündoğdu, Irmak Şahbaz and Özge Demet Özçete for their skillful technical assistance.
Conceived and designed the experiments: SL OO HO GA ANB. Performed the experiments: SL OO OU ZSA. Analyzed the data: SL OO OU ZSA HO. Contributed reagents/materials/analysis tools: AO YP FD PO FK. Wrote the paper: SL OO AO HO GA ANB.
- 1. Kiernan MC, Vucic S, Cheah BC, Turner MR, Eisen A, et al. (2011) Amyotrophic lateral sclerosis. Lancet 377: 942–955. doi: 10.1016/s0140-6736(10)61156-7
- 2. Rosen DR (1993) Mutations in Cu/Zn superoxide dismutase gene are associated with familial amyotrophic lateral sclerosis. Nature 364: 362. doi: 10.1038/364362c0
- 3. Lagier-Tourenne C, Polymenidou M, Cleveland DW (2010) TDP-43 and FUS/TLS: emerging roles in RNA processing and neurodegeneration. Hum Mol Genet 19: R46–64. doi: 10.1093/hmg/ddq137
- 4. Kwiatkowski TJ Jr, Bosco DA, Leclerc AL, Tamrazian E, Vanderburg CR, et al. (2009) Mutations in the FUS/TLS gene on chromosome 16 cause familial amyotrophic lateral sclerosis. Science 323: 1205–1208. doi: 10.1126/science.1166066
- 5. Vance C, Rogelj B, Hortobagyi T, De Vos KJ, Nishimura AL, et al. (2009) Mutations in FUS, an RNA processing protein, cause familial amyotrophic lateral sclerosis type 6. Science 323: 1208–1211. doi: 10.1126/science.1165942
- 6. Strong MJ (2010) The evidence for altered RNA metabolism in amyotrophic lateral sclerosis (ALS). J Neurol Sci 288: 1–12. doi: 10.1016/j.jns.2009.09.029
- 7. Colombrita C, Onesto E, Tiloca C, Ticozzi N, Silani V, et al. (2011) RNA-binding proteins and RNA metabolism: a new scenario in the pathogenesis of Amyotrophic lateral sclerosis. Arch Ital Biol 149: 83–99.
- 8. Deng HX, Chen W, Hong ST, Boycott KM, Gorrie GH, et al. (2011) Mutations in UBQLN2 cause dominant X-linked juvenile and adult-onset ALS and ALS/dementia. Nature 477: 211–215. doi: 10.1038/nature10353
- 9. DeJesus-Hernandez M, Mackenzie IR, Boeve BF, Boxer AL, Baker M, et al. (2011) Expanded GGGGCC hexanucleotide repeat in noncoding region of C9ORF72 causes chromosome 9p-linked FTD and ALS. Neuron 72: 245–256. doi: 10.1016/j.neuron.2011.09.011
- 10. Renton AE, Majounie E, Waite A, Simon-Sanchez J, Rollinson S, et al. (2011) A hexanucleotide repeat expansion in C9ORF72 is the cause of chromosome 9p21-linked ALS-FTD. Neuron 72: 257–268. doi: 10.1016/j.neuron.2011.09.010
- 11. Cronin S, Berger S, Ding J, Schymick JC, Washecka N, et al. (2008) A genome-wide association study of sporadic ALS in a homogenous Irish population. Hum Mol Genet 17: 768–774. doi: 10.1093/hmg/ddm361
- 12. Schymick JC, Scholz SW, Fung HC, Britton A, Arepalli S, et al. (2007) Genome-wide genotyping in amyotrophic lateral sclerosis and neurologically normal controls: first stage analysis and public release of data. Lancet Neurol 6: 322–328. doi: 10.1016/s1474-4422(07)70037-6
- 13. van Es MA, Veldink JH, Saris CG, Blauw HM, van Vught PW, et al. (2009) Genome-wide association study identifies 19p13.3 (UNC13A) and 9p21.2 as susceptibility loci for sporadic amyotrophic lateral sclerosis. Nat Genet 41: 1083–1087. doi: 10.1038/ng.442
- 14. Elden AC, Kim HJ, Hart MP, Chen-Plotkin AS, Johnson BS, et al. (2010) Ataxin-2 intermediate-length polyglutamine expansions are associated with increased risk for ALS. Nature 466: 1069–1075. doi: 10.1038/nature09320
- 15. Chen Y, Huang R, Yang Y, Chen K, Song W, et al.. (2011) Ataxin-2 intermediate-length polyglutamine: a possible risk factor for Chinese patients with amyotrophic lateral sclerosis. Neurobiol Aging 32: 1925 e1921–1925.
- 16. Corrado L, Mazzini L, Oggioni GD, Luciano B, Godi M, et al. (2011) ATXN-2 CAG repeat expansions are interrupted in ALS patients. Hum Genet 130: 575–580. doi: 10.1007/s00439-011-1000-2
- 17. Daoud H, Belzil V, Martins S, Sabbagh M, Provencher P, et al. (2011) Association of long ATXN2 CAG repeat sizes with increased risk of amyotrophic lateral sclerosis. Arch Neurol 68: 739–742. doi: 10.1001/archneurol.2011.111
- 18. Lee T, Li YR, Ingre C, Weber M, Grehl T, et al. (2011) Ataxin-2 intermediate-length polyglutamine expansions in European ALS patients. Hum Mol Genet 20: 1697–1700. doi: 10.1093/hmg/ddr045
- 19. Ross OA, Rutherford NJ, Baker M, Soto-Ortolaza AI, Carrasquillo MM, et al. (2011) Ataxin-2 repeat-length variation and neurodegeneration. Hum Mol Genet 20: 3207–3212. doi: 10.1093/hmg/ddr227
- 20. Van Damme P, Veldink JH, van Blitterswijk M, Corveleyn A, van Vught PW, et al. (2011) Expanded ATXN2 CAG repeat size in ALS identifies genetic overlap between ALS and SCA2. Neurology 76: 2066–2072. doi: 10.1212/wnl.0b013e31821f445b
- 21. Gispert S, Kurz A, Waibel S, Bauer P, Liepelt I, et al. (2012) The modulation of Amyotrophic Lateral Sclerosis risk by Ataxin-2 intermediate polyglutamine expansions is a specific effect. Neurobiol Dis 45: 356–361. doi: 10.1016/j.nbd.2011.08.021
- 22. Lee T, Li YR, Chesi A, Hart MP, Ramos D, et al. (2011) Evaluating the prevalence of polyglutamine repeat expansions in amyotrophic lateral sclerosis. Neurology 76: 2062–2065. doi: 10.1212/wnl.0b013e31821f4447
- 23. Ralser M, Nonhoff U, Albrecht M, Lengauer T, Wanker EE, et al. (2005) Ataxin-2 and huntingtin interact with endophilin-A complexes to function in plastin-associated pathways. Hum Mol Genet 14: 2893–2909. doi: 10.1093/hmg/ddi321
- 24. Satterfield TF, Pallanck LJ (2006) Ataxin-2 and its Drosophila homolog, ATX2, physically assemble with polyribosomes. Hum Mol Genet 15: 2523–2532. doi: 10.1093/hmg/ddl173
- 25. Nonhoff U, Ralser M, Welzel F, Piccini I, Balzereit D, et al. (2007) Ataxin-2 interacts with the DEAD/H-box RNA helicase DDX6 and interferes with P-bodies and stress granules. Mol Biol Cell 18: 1385–1396. doi: 10.1091/mbc.e06-12-1120
- 26. van de Loo S, Eich F, Nonis D, Auburger G, Nowock J (2009) Ataxin-2 associates with rough endoplasmic reticulum. Exp Neurol 215: 110–118. doi: 10.1016/j.expneurol.2008.09.020
- 27. Nonis D, Schmidt MH, van de Loo S, Eich F, Dikic I, et al. (2008) Ataxin-2 associates with the endocytosis complex and affects EGF receptor trafficking. Cell Signal 20: 1725–1739. doi: 10.1016/j.cellsig.2008.05.018
- 28. Huynh DP, Figueroa K, Hoang N, Pulst SM (2000) Nuclear localization or inclusion body formation of ataxin-2 are not necessary for SCA2 pathogenesis in mouse or human. Nat Genet 26: 44–50.
- 29. Pang JT, Giunti P, Chamberlain S, An SF, Vitaliani R, et al. (2002) Neuronal intranuclear inclusions in SCA2: a genetic, morphological and immunohistochemical study of two cases. Brain 125: 656–663. doi: 10.1093/brain/awf060
- 30. Huynh DP, Nguyen DT, Pulst-Korenberg JB, Brice A, Pulst SM (2007) Parkin is an E3 ubiquitin-ligase for normal and mutant ataxin-2 and prevents ataxin-2-induced cell death. Exp Neurol 203: 531–541. doi: 10.1016/j.expneurol.2006.09.009
- 31. Auburger GW (2012) Spinocerebellar ataxia type 2. Handb Clin Neurol 103: 423–436. doi: 10.1016/b978-0-444-51892-7.00026-7
- 32. Lastres-Becker I, Rub U, Auburger G (2008) Spinocerebellar ataxia 2 (SCA2). Cerebellum 7: 115–124. doi: 10.1007/s12311-008-0019-y
- 33. Gwinn-Hardy KA, Crook R, Lincoln S, Adler CH, Caviness JN, et al. (2000) A kindred with Parkinson’s disease not showing genetic linkage to established loci. Neurology 54: 504–507. doi: 10.1212/wnl.54.2.504
- 34. Yu Z, Zhu Y, Chen-Plotkin AS, Clay-Falcone D, McCluskey L, et al. (2011) PolyQ repeat expansions in ATXN2 associated with ALS are CAA interrupted repeats. PLoS One 6: e17951. doi: 10.1371/journal.pone.0017951
- 35. Choudhry S, Mukerji M, Srivastava AK, Jain S, Brahmachari SK (2001) CAG repeat instability at SCA2 locus: anchoring CAA interruptions and linked single nucleotide polymorphisms. Hum Mol Genet 10: 2437–2446. doi: 10.1093/hmg/10.21.2437
- 36. Brooks BR, Miller RG, Swash M, Munsat TL (2000) El Escorial revisited: revised criteria for the diagnosis of amyotrophic lateral sclerosis. Amyotroph Lateral Scler Other Motor Neuron Disord 1: 293–299. doi: 10.1080/146608200300079536
- 37. Purcell S, Neale B, Todd-Brown K, Thomas L, Ferreira MA, et al. (2007) PLINK: a tool set for whole-genome association and population-based linkage analyses. Am J Hum Genet 81: 559–575. doi: 10.1086/519795
- 38. Barrett JC, Fry B, Maller J, Daly MJ (2005) Haploview: analysis and visualization of LD and haplotype maps. Bioinformatics 21: 263–265. doi: 10.1093/bioinformatics/bth457
- 39. Gabriel SB, Schaffner SF, Nguyen H, Moore JM, Roy J, et al. (2002) The structure of haplotype blocks in the human genome. Science 296: 2225–2229. doi: 10.1126/science.1069424
- 40. Lee J K (2010) Statistical Bioinformatics: A Guide for Life and Biomedical Science Researchers. New Jersey: Wiley-Blackwell. 293–94.
- 41. Soraru G, Clementi M, Forzan M, Orsetti V, D’Ascenzo C, et al. (2011) ALS risk but not phenotype is affected by ataxin-2 intermediate length polyglutamine expansion. Neurology 76: 2030–2031. doi: 10.1212/wnl.0b013e31821e557a
- 42. Van Langenhove T, van der Zee J, Engelborghs S, Vandenberghe R, Santens P, et al.. (2011) Ataxin-2 polyQ expansions in FTLD-ALS spectrum disorders in Flanders-Belgian cohorts. Neurobiol Aging.
- 43. Takaki S, Sauer K, Iritani BM, Chien S, Ebihara Y, et al. (2000) Control of B cell production by the adaptor protein lnk. Definition Of a conserved family of signal-modulating proteins. Immunity 13: 599–609. doi: 10.1016/s1074-7613(00)00060-1
- 44. Ahmed Z, Pillay TS (2001) Functional effects of APS and SH2-B on insulin receptor signalling. Biochem Soc Trans 29: 529–534. doi: 10.1042/bst0290529
- 45. O’Brien KB, O’Shea JJ, Carter-Su C (2002) SH2-B family members differentially regulate JAK family tyrosine kinases. J Biol Chem 277: 8673–8681. doi: 10.1074/jbc.m109165200
- 46. Devalliere J, Charreau B (2011) The adaptor Lnk (SH2B3): an emerging regulator in vascular cells and a link between immune and inflammatory signaling. Biochem Pharmacol 82: 1391–1402. doi: 10.1016/j.bcp.2011.06.023
- 47. Wang TC, Chiu H, Chang YJ, Hsu TY, Chiu IM, et al. (2011) The adaptor protein SH2B3 (Lnk) negatively regulates neurite outgrowth of PC12 cells and cortical neurons. PLoS One 6: e26433. doi: 10.1371/journal.pone.0026433
- 48. Alcina A, Vandenbroeck K, Otaegui D, Saiz A, Gonzalez JR, et al. (2010) The autoimmune disease-associated KIF5A, CD226 and SH2B3 gene variants confer susceptibility for multiple sclerosis. Genes Immun 11: 439–445. doi: 10.1038/gene.2010.30