Methicillin-resistant Staphylococcus aureus (MRSA) infection is an important public health issue. The study aimed to determine the prevalence of ocular infections caused by MRSA and to identify the clinical characteristics and antibiotic susceptibility of ocular MRSA infections by comparing those of ocular methicillin-sensitive S. aureus (MSSA) infections.
The medical records of the patients (n = 519) with culture-proven S. aureus ocular infections seen between January 1, 1999 and December 31, 2008 in Chang Gung Memorial Hospital were retrospectively reviewed. Two hundred and seventy-four patients with MRSA and 245 with MSSA ocular infections were identified. The average rate of MRSA in S. aureus infections was 52.8% and the trend was stable over the ten years (P value for trend = 0.228). MRSA ocular infections were significantly more common among the patients with healthcare exposure (P = 0.024), but 66.1% (181/274) patients with MRSA ocular infections had no healthcare exposure. The most common clinical presentation for both MRSA and MSSA ocular infections was keratitis; MRSA and MSSA caused a similar disease spectrum except for lid infections. MRSA was significantly more resistant than MSSA to clindamycin, erythromycin and sulfamethoxazole/trimethoprim (all P<0.001).
We demonstrated a paralleled trend of ocular MRSA infection in a highly prevalent MRSA country by hospital-based survey. Except for lid disorder, MRSA shared similar spectrum of ocular pathology with MSSA. Since S. aureus is a common ocular pathogen, our results raise clinician’s attention to the existence of highly prevalent MRSA.
Citation: Chuang C-C, Hsiao C-H, Tan H-Y, Ma DH-K, Lin K-K, et al. (2012) Staphylococcus aureus Ocular Infection: Methicillin-Resistance, Clinical Features, and Antibiotic Susceptibilities. PLoS ONE 7(8): e42437. doi:10.1371/journal.pone.0042437
Editor: Mark Alexander Webber, University of Birmingham, United Kingdom
Received: March 8, 2012; Accepted: July 9, 2012; Published: August 7, 2012
Copyright: © Chuang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported in part by Chang Gung Memorial Hospital, Linkou, Taiwan, grant number CMRPG381511. No additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Staphylococcus aureus is among the most important and commonly isolated human bacterial pathogens. S. aureus isolates resistant to methicillin, usually also resistant to other β-lactam antimicrobial drugs, are termed methicillin-resistant S. aureus (MRSA). MRSA, first identified in the 1960s, was traditionally associated with healthcare facilities, but is now a dominant pathogen in community-associated infections.  MRSA is of particular concern as a serious cause of morbidity and mortality worldwide, because of its multiple drug resistance, leaving limited treatment options and its believed increasing prevalence.
Previous data on ocular MRSA infections were generally limited to case reports and small case series, – but a variety of more recent publications have presented substantially larger analyses of ocular MRSA in the United States. – Case series of catastrophic eye infections caused by MRSA has been reported recently in patients after refractive and cataract surgery. , ,  According to the report from American Society of Cataract and Refractive Surgery, MRSA has replaced nontuberculous mycobacteria to be the most common pathogen causing infections after laser-assisted in situ keratomileusis.  MRSA has been reported to account for 18.2% (6/33) culture-proven endophthalmitis in a referral vitreoretinal practice.  The proportion of MRSA in ocular S. aureus infections from a single institution varies from 3% to 30%, with some reports showing increasing incidence of MRSA. – The Surveillance Network, which monitors antimicrobial susceptibility patterns of bacterial pathogens in the United States, reported an increase in the proportion of MRSA among S. aureus ocular infections, from 29.5% in 2000 to 41.6% in 2005, which showed MRSA is a rising menace in ocular field.  In Taiwan, the rate of MRSA among S. aureus clinical isolates, was about 60% during 1997–2000, ,  a rate higher than that reported in other regions of the world,  but the rate of ocular infections caused by MRSA remains unknown.
Here, we conducted a 10-year retrospective study to determine the rate of ocular MRSA infections and to identify the clinical characteristics and antibiotic susceptibility of ocular MRSA infection by comparing those of ocular methicillin sensitive S. aureus (MSSA) infections seen in Chang Gung Memorial Hospital, a 3000-bed tertiary referral hospital in Taiwan.
The study was approved by the institute review boards from Chang Gung Memorial Hospital, which allowed retrieve of the patients list from the electronic microbiology database, review of the medical information. A waiver of consent was granted given the retrospective nature of the project and anonymous analysis of the data.
Participants and Procedures
From the microbiologic laboratory database, we identified all the patients with an ocular specimen, collected by ophthalmologists, sent for bacterial culture and positive for S. aureus between January 1, 1999 and December 31, 2008. We included no more than one isolate per patient. We determined susceptibility of the isolates to seven antibiotics (oxacillin, penicillin, erythromycin, clindamycin, trimethoprim/sulfamethoxazole, vancomycin and teicoplanin) using the disc diffusion method according to the Clinical and Laboratory Standard Institute (CLSI) standards for antimicrobial susceptibility testing. We used oxacillin, which was replaced by cefoxitin since March 2006, to test for β-lactam antibiotic resistance. We reviewed patient charts to collect demographic and clinical information. Based on the structures involved, we classed ocular infections into one of seven diagnoses: conjunctivitis, keratitis, lid disorder, lacrimal system disorder, wound infection, endophthalmitis and other (e.g., blebitis, buckle or implant infection and sclera ulcer). If the chart showed more than one diagnosis, we chose the primary pathology or the more severe diagnosis. If the patients had either: 1) a MRSA infection identified after 48 hours of admission to a hospital; 2) a history of hospitalization, surgery, dialysis, or residence in a long-term care facility within one year of the MRSA culture date; 3) a permanent indwelling catheter or percutaneous medical device present at the time of culture; or 4) a known positive culture for MRSA prior to the study period, they were thought to have healthcare exposure .
Patients with MSSA ocular infections constituted the control group and patients with MRSA ocular infections were the study group. Nominal variables were analyzed with the chi square test. Continuous variables were analyzed with Student’s t test. Trend analysis was performed by the chi square test for trends. For comparison, we grouped the data into two five-year study periods, from January 1999 to December 2003 and from January 2004 to December 2008. All analyses were two-tailed, and P<0.05 was considered statistically significant. We performed all statistical analyses using R (The R Foundation for Statistical Computing, Vienna, Austria; available at: http://www.R-project.org).
During the 10-year study period, S. aureus was isolated from 519 patients. Of these, 274 were MRSA and 245 were MSSA.
The Rate of MRSA in S. aureus Ocular Infections
As illustrated in Figure 1, the average annual rate of MRSA among ocular S. aureus infections was 52.8%, ranging from 41.9% in 2000 to 76.5% in 2006, and the trend was stable for the 10-year interval (P value for trend = 0.228).
Figure 1. Percentage of patients with ocular methicillin-resistant Staphylococcus aureus (MRSA) by year.doi:10.1371/journal.pone.0042437.g001
Characteristics of Ocular MRSA and MSSA Infections (Table 1)
The groups had similar median ages, 41.3 years (range, 1 month to 89 years) for patients with ocular MRSA infections and 37.3 years (range, 2 months to 84 years) for those with ocular MSSA infections. Of the 281 males and 238 females infected, significantly more males than females had MSSA (P = 0.036). The groups did not differ in eye involvement (laterality). Ninety three patients (93/274, 33.9%) with MRSA ocular infections and 60 patients (60/245, 24.5%) with MSSA ocular infections had healthcare exposure; MRSA infections were significant more common among the patients with healthcare exposure (P = 0.024). Patients with MRSA did not differ significantly from MSSA patients in the presence of underlying comorbidities or use of immunosuppressants. In MRSA group, the rate of the patients with healthcare exposure significantly decreased from 44.8% to 25.9% (P = 0.002) in the most recent five years. There was no change in other demographics of both MRSA and MSSA groups between the two study periods.
Table 1. Comparison of demographics and characteristics of ocular 274 methicillin-resistant Staphylococcus aureus and 245 methicillin-sensitive Staphylococcus aureus infections.doi:10.1371/journal.pone.0042437.t001
Clinical Diagnoses Associated with Ocular MRSA and MSSA Infections (Table 2)
Keratitis was the most common ocular diagnosis in all patients and accounted for 36.1% and 40.0% of cases of MRSA and MSSA, respectively. About 20% of patients in both groups had conjunctivitis. In MRSA group, the rate of lid disorder significantly increased from 23.9% to 76.1% (P<0.001), while the rate of keratitis significantly decreased from 55.6% to 44.4% during the last five years (P = 0.001). The rate of various diagnoses caused by MSSA did not change between the two study periods. By comparison, patients with MRSA infections presented with lid disorder significantly more often than patients with MSSA infections (24.5% vs. 16.7%, P = 0.040), but the rate of patients presenting with other diagnoses did not differ significantly between both groups. MRSA and MSSA did not differ in their association with a diagnosis of vision-threatening disorders (i.e., keratitis, orbital cellulitis or endophthalmitis).
Table 2. Comparison of clinical diagnoses associated with ocular 274 methicillin-resistant Staphylococcus aureus and 245 methicillin-sensitive Staphylococcus aureus infections.doi:10.1371/journal.pone.0042437.t002
Antibiotics Susceptibility of MRSA and MSSA for Ocular Infections (Table 3)
As expected, MRSA was significantly more resistant than MSSA to several antibiotics including clindamycin, erythromycin and sulfamethoxazole/trimethoprim. Seventy-eight percent of MRSA isolates were susceptible to sulfamethoxazole/trimethoprim. All MRSA and MSSA isolates were susceptible to vancomycin and teicoplanin. Susceptibility of both MRSA and MSSA groups to all seven antibiotics did not change significantly in the most recent five years.
To our knowledge, the current study represents the largest reported case series of ocular S. aureus infections. Our findings show that 52.8% of ocular S. aureus infections were MRSA and the trend was stable over a 10-year interval at our hospital. MRSA ocular infections were significant more common among the patients with healthcare exposure. Except for lid disorder, MRSA and MSSA caused a similar disease spectrum and severity of ocular disorders. MRSA was significantly more resistant than MSSA to clindamycin, erythromycin and sulfamethoxazole/trimethoprim.
Our data showed a relatively high and stable rate of ocular isolates of MRSA in our hospital, although most other studies reported increasing MRSA prevalence. Blomquist showed an increase in the incidence of ophthalmic MRSA from 12% to 33% over a 5-year period (2000 to 2004) in an urban health care system in the United States.  Freidlin et al. reported a similar increase in the proportion of ocular MRSA infections from 4.1% in 1998 to 1999 to 16.7% in 2005–2006.  The Surveillance Network demonstrated an increase in the proportion of culture-positive ocular MRSA from 29.5% in 2000 to 41.6% in 2005 in serious S. aureus ocular infections, predicting that MRSA could be more common than MSSA within two to three years, based on the rate of increase.  Instead, our results were in line with the prevalence reported for our hospital and Taiwan. MRSA was first documented in the early 1980s in Taiwan and its prevalence has increased remarkably since.  Based on data from 12 major hospitals in Taiwan, MRSA accounted for 53% to 83% of all S. aureus clinical isolates in 2000.  In our hospital, an average of 63.9% of all S. aureus infections were MRSA (range, 59.0% to 70.0%), with no trend change over a 10-year interval, similar to ocular infections due to S. aureus. These data indicate that MRSA is prevalent and with a stable rate of S. aureus infections in our hospital, perhaps in the plateau stage, in the past decade.
As expected, a statistically significantly greater number of the patients with MRSA ocular infections had healthcare exposure than those with MSSA ocular infections in this study. However, it is noteworthy that two thirds of the patients (181/274, 66.1%) with MRSA ocular infections had no healthcare exposure, which meant the isolates were potentially community associated. MRSA was once associated with healthcare facilities, but more recent reports showed an increasing frequency of isolates from community-associated MRSA infections in Taiwan as elsewhere. – Since most ophthalmologic patients are seen and treated as outpatients instead of inpatients, community-associated MRSA may play an important role in MRSA ocular infections. Further analysis of community associated ocular MRSA infection has been conducted and published separately .
In our study, the most common presentation of ocular MRSA infections was keratitis (36.1%), followed by lid disorder (24.5%) and conjunctivitis (20.1%); nearly half (47.8%) of ocular MRSA infections were vision-threatening. However, previous large case series studies showed that the most common manifestation of ophthalmic MRSA infection was conjunctivitis ,  or lid disorder ; vision-threatening infections were relatively uncommon. – Our results may have differed due to selection bias, because there were more severe cases in our hospital, a tertiary referred center. Also, physicians may differ in which cases they sent for diagnostic testing; some may tend to culture only the most serious cases. Third, we may exclude some patients with ocular infections, while the cultures were not done by ophthalmologists.
MRSA is believed to cause a more severe disease than MSSA, but this observation has not reached consensus. ,  Our results did not show that MRSA caused more severe ocular diseases than MSSA; this agrees with Freidlin’s study, which reported MRSA and MSSA caused similar eye disease.  We did find that patients with MRSA were more likely to have lid infections. In addition, the rate of lid disorder caused by MRSA significantly increased, but the rate of keratitis caused by MRSA significantly decreased during the last five years. Community-associated MRSA has a reported predilection for causing skin and soft tissue infections, ,  and we also found that lid and lacrimal system disorders were more common, but keratitis, endophthalmitis and wound infection were less common among community associated MRSA cases than healthcare associated MRSA cases.  Thus, 66.1% of patients with MRSA ocular infections were community-associated and the paralleled significant increase in the rate of the MRSA patients without healthcare exposure (i.e. community-associated MRSA) in the most recent five years may explain these results.
According to antibiotic susceptibility profiles, vancomycin was the most active agent against ocular MRSA isolates, whereas sulfamethoxazole-trimethoprim retained some degree of activity against MRSA, but was less effective than in previous studies. , ,  Although vancomycin retains extremely high efficacy against MRSA, S. aureus with reduced susceptibility to vancomycin was identified.  Since prior vancomycin use is a risk factor for MRSA with reduced vancomycin susceptibility,  and no convincing evidence shows that routine vancomycin prophylaxis is effective in elective cataract surgery,  we recommend that ophthalmologists follow guideline of the Centers for Disease Control and Prevention  and the American Academy of Ophthalmology  against the routine use of vancomycin for prophylaxis to halt the spread of resistance. Several recent studies have reported that MRSA has a high rate of in vitro resistance to fluoroquinolones, including new generation ones, the most popular empiric therapy in ocular infections. , , , , , ,  We did not test fluoroquinolones in our study because they were not included in the recommended list of antibiotics published by the CLSI. In Taiwan, National data from 2000 (TSAR program) has demonstrated 40% S. aureus (including MSSA and MRSA) in vitro resistance to ciprofloxacin.  We may extend the antibiotic susceptibility profiles to include commonly used topical antibiotics in future studies.
Eight ocular MRSA isolates from pediatric patients were stored and available for genotyping analysis, including pulsed-filed gel electrophoresis (PFGE) typing, SCCmec elements and the detection of PVL genes in this study. The only one healthcare associated MRSA isolate was characterized as PFGE type A/SCCmec IIIA/PVL-negative, which was compatible with those of healthcare associated MRSA isolates (sequence type (ST) 239, Hungary clone) in our previous studies. ,  Four of seven isolates classified as community-associated MRSA were characterized by PFGE type D/SCCmec VT/PVL positive, and the other three were characterized by PFGE type C/SCCmec IV/PVL-negative. Both clones shared the common genetic characteristics of community-associated MRSA strains in Taiwan (ST 59, Taiwan clone). ,  These molecular results, though limited, further confirmed the classification of community-associated and healthcare-associated MRSA based on epidemiologic data in the present study was confident.
The currently recommended disc diffusion method to determine resistance against methicillin for S. aureus uses cefoxitin rather than oxacillin, because cefoxitin results are easier to interpret and are more sensitive for the detection of mecA-mediated resistance than oxacillin results, especially for identifying community associated MRSA, which may have low MIC to oxacillin. ,  Our Clinical Microbiology Laboratories started using cefoxitin instead of oxacillin to test for β-lactam antibiotic resistance since March 2006 as the performance standard from the CLSI was revised. When we made a comparison between MRSA and MSSA ocular infections from March 2006 to the end of 2008, the results were the same as those in the 10-year study interval except there was no significant difference in the proportion of the patients with healthcare exposure in MRSA and MSSA. It was probably due to the increase in community-associated MRSA over time in our hospital .
Our study has the inherent flaws of a retrospective design. The patient selection criteria may influence data interpretation. Since our study population attended a referral-based, tertiary-care hospital, results may not be applicable to other populations. In addition, we used oxacillin/cefoxitin testing as a surrogate for detecting the mecA gene in the identification of resistant species of Staphylococcus; and we distinguished community-associated MRSA from healthcare-associated MRSA based on epidemiological differences, not genetic characterization. Our Clinical Microbiology Laboratories retain only isolates from blood for long-term storage, so we did not have the ocular isolates for further analysis. Thus, misclassification bias may limit applicability of the results. Furthermore, resistance found in vitro based on serum systemic standards does not always correlate with clinical resistance, because there are no susceptibility standards for topical therapy. Finally, the scope of our study primarily focused on the epidemiology and included a broad spectrum of diseases, so the treatment and visual outcomes were not intended to be discussed.
Infectious diseases may differ by regions in epidemiologic patterns, spectrum and severity of disease, and profiles of antibiotic susceptibility. In this 10-year retrospective study, we found that MRSA was common in ocular S. aureus infections in our hospital, which paralleled trends of systemic MRSA infections, in this highly prevalent MRSA country. While we failed to demonstrate a difference in virulence between MRSA and MSSA, vision-threatening disorders were common in both. All MRSA isolates were susceptible to vancomycin. Establishing the baseline characteristics of MRSA ocular infections helps us track future progress and choose the most appropriate treatment.
The authors thank Mr. Lin Yu-Jr in the Biostatistical Center for Clinical Research, Chang Gung Memorial Hospital, Taiwan for the assistance in statistical analyses.
Conceived and designed the experiments: CCC CHH HYT. Performed the experiments: CCC CHH. Analyzed the data: CCC CHH YCH. Contributed reagents/materials/analysis tools: DHKM KKL CJC YCH. Wrote the paper: CHH YCH.
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